(Circular) (Six-base) MAPSORT of: pFN21AB3540.seq Check: 638 from: 1 to: 8230 pFN21AB3540 8230 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6040 278 Size: 53 83 186 936 4504 2468 Fragments arranged by size: 4504 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2721 4922 5573 1937 Size: 784 2201 651 4594 Fragments arranged by size: 4594 2201 784 651 Acc65I G'GTAC_C Cuts at: 4020 5551 4020 Size: 1531 6699 AclI AA'CG_TT Cuts at: 1563 6357 6730 1563 Size: 4794 373 3063 Fragments arranged by size: 4794 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2395 3426 6293 7677 7945 1632 Size: 763 1031 2867 1384 268 1917 Fragments arranged by size: 2867 1917 1384 1031 763 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8033 AflIII A'CryG_T Cuts at: 1203 3777 7129 8106 1203 Size: 2574 3352 977 1327 Fragments arranged by size: 3352 2574 1327 977 AgeI A'CCGG_T Cuts at: 7061 7061 Size: 8230 AhdI GACnn_n'nnGTC Cuts at: 6959 6959 Size: 8230 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8230 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3362 4126 5246 1184 Size: 2178 764 1120 4168 Fragments arranged by size: 4168 2178 1120 764 AlwNI CAG_nnn'CTG Cuts at: 5017 5197 5973 7545 5017 Size: 180 776 1572 5702 Fragments arranged by size: 5702 1572 776 180 ApaLI G'TGCA_C Cuts at: 6287 7443 6287 Size: 1156 7074 ApoI r'AATT_y Cuts at: 1493 2021 2493 2775 3542 3548 3833 3995 4214 Size: 528 472 282 767 6 285 162 219 Cuts at: 4214 4523 4822 5007 5438 5539 5765 5917 8026 Size: 309 299 185 431 101 226 152 2109 Cuts at: 8026 1493 Size: 1697 Fragments arranged by size: 2109 1697 767 528 472 431 309 299 285 282 226 219 185 162 152 101 6 AseI AT'TA_AT Cuts at: 160 1032 6784 160 Size: 872 5752 1606 Fragments arranged by size: 5752 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8230 AvaI C'yCGr_G Cuts at: 1870 1943 5555 1870 Size: 73 3612 4545 Fragments arranged by size: 4545 3612 73 BamHI G'GATC_C Cuts at: 2230 4359 5560 5927 2230 Size: 2129 1201 367 4533 Fragments arranged by size: 4533 2129 1201 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4020 4160 5551 7006 618 Size: 325 217 627 2233 140 1391 1455 1842 Fragments arranged by size: 2233 1842 1455 1391 627 325 217 140 BanII G_rGCy'C Cuts at: 1391 2607 4683 5549 1391 Size: 1216 2076 866 4072 Fragments arranged by size: 4072 2076 1216 866 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2567 2908 3018 3111 3774 3824 4770 Size: 971 668 341 110 93 663 50 946 Cuts at: 4770 928 Size: 4388 Fragments arranged by size: 4388 971 946 668 663 341 110 93 50 BbvCI CC'TCA_GC Cuts at: 5103 5132 5103 Size: 29 8201 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2995 3029 3845 3879 5455 5489 6421 Size: 34 1058 34 816 34 1576 34 932 Cuts at: 6421 6455 1903 Size: 34 3678 Fragments arranged by size: 3678 1576 1058 932 816 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6122 7332 6122 Size: 1210 7020 BclI T'GATC_A Cuts at: 1548 3708 5085 1548 Size: 2160 1377 4693 Fragments arranged by size: 4693 2160 1377 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2804 6841 136 Size: 107 122 71 631 605 1132 4037 1525 Fragments arranged by size: 4037 1525 1132 631 605 122 107 71 BglII A'GATC_T Cuts at: 2317 2674 3280 8225 2317 Size: 357 606 4945 2322 Fragments arranged by size: 4945 2322 606 357 BlpI GC'TnA_GC Cuts at: 5647 5647 Size: 8230 Bme1580I G_kGCm'C Cuts at: 1790 4602 6291 7447 1790 Size: 2812 1689 1156 2573 Fragments arranged by size: 2812 2573 1689 1156 BmgBI CAC'GTC Cuts at: 1318 4597 1318 Size: 3279 4951 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2574 2906 4596 Size: 416 53 83 399 24 1157 332 1690 Cuts at: 4596 5961 6919 442 Size: 1365 958 1753 Fragments arranged by size: 1753 1690 1365 1157 958 416 399 332 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8230 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3474 6890 1239 Size: 2235 3416 2579 Fragments arranged by size: 3416 2579 2235 Bpu10I CC'TnA_GC Cuts at: 5103 5132 5103 Size: 29 8201 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2429 2546 4333 4734 6355 7220 7518 2429 Size: 117 1787 401 1621 865 298 3141 Fragments arranged by size: 3141 1787 1621 865 401 298 117 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5115 6893 882 Size: 617 3616 1778 2219 Fragments arranged by size: 3616 2219 1778 617 BsaAI yAC'GTr Cuts at: 493 1204 4168 4208 6021 493 Size: 711 2964 40 1813 2702 Fragments arranged by size: 2964 2702 1813 711 40 BsaBI GATnn'nnATC Cuts at: 8224 8224 Size: 8230 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6037 6419 7054 275 Size: 53 83 186 936 4504 382 635 1451 Fragments arranged by size: 4504 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6663 7061 7335 7482 7893 6663 Size: 398 274 147 411 7000 Fragments arranged by size: 7000 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3036 3066 3873 3903 4482 Size: 30 165 30 1633 30 807 30 579 Cuts at: 4482 4512 1178 Size: 30 4896 Fragments arranged by size: 4896 1633 807 579 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2099 3515 3539 5120 1186 Size: 913 1416 24 1581 4296 Fragments arranged by size: 4296 1581 1416 913 24 BseYI C'CCAG_C Cuts at: 3087 3469 3720 5190 7433 7929 3087 Size: 382 251 1470 2243 496 3388 Fragments arranged by size: 3388 2243 1470 496 382 251 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4139 4861 5111 1163 Size: 573 2403 722 250 4282 Fragments arranged by size: 4282 2403 722 573 250 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2213 5680 6441 7469 1540 Size: 275 186 212 3467 761 1028 2301 Fragments arranged by size: 3467 2301 1028 761 275 212 186 BsiHKAI G_wGCw'C Cuts at: 4683 5239 6291 6376 7447 4683 Size: 556 1052 85 1071 5466 Fragments arranged by size: 5466 1071 1052 556 85 BsmI GAATG_Cn' Cuts at: 2192 5741 5834 2192 Size: 3549 93 4588 Fragments arranged by size: 4588 3549 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2607 4602 4683 5239 5549 6291 6376 Size: 399 817 1995 81 556 310 742 85 Cuts at: 6376 7447 1391 Size: 1071 2174 Fragments arranged by size: 2174 1995 1071 817 742 556 399 310 85 81 BspEI T'CCGG_A Cuts at: 7893 7893 Size: 8230 BspHI T'CATG_A Cuts at: 4041 4927 6119 7849 4041 Size: 886 1192 1730 4422 Fragments arranged by size: 4422 1730 1192 886 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4111 5133 5585 844 Size: 3267 1022 452 3489 Fragments arranged by size: 3489 3267 1022 452 BsrBI CCG'CTC Cuts at: 1394 1823 6117 1394 Size: 429 4294 3507 Fragments arranged by size: 4294 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2381 2814 3144 4956 6725 6899 65 Size: 1183 1133 433 330 1812 1769 174 1396 Fragments arranged by size: 1812 1769 1396 1183 1133 433 330 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6874 7061 1700 Size: 110 5064 187 2869 Fragments arranged by size: 5064 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3039 3143 96 Size: 2943 104 5183 Fragments arranged by size: 5183 2943 104 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8230 BssSI C'ACGA_G Cuts at: 5177 6290 7302 5177 Size: 1113 1012 6105 Fragments arranged by size: 6105 1113 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8230 BstBI TT'CG_AA Cuts at: 4408 7034 4408 Size: 2626 5604 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8230 BstXI CCAn_nnnn'nTGG Cuts at: 2242 5401 2242 Size: 3159 5071 BstYI r'GATC_y Cuts at: 1972 2230 2317 2674 2758 3280 3292 4359 4513 Size: 258 87 357 84 522 12 1067 154 Cuts at: 4513 5560 5927 6312 6329 7781 7867 7879 8225 Size: 1047 367 385 17 1452 86 12 346 Cuts at: 8225 1972 Size: 1977 Fragments arranged by size: 1977 1452 1067 1047 522 385 367 357 346 258 154 87 86 84 17 12 12 BtgI C'CryG_G Cuts at: 513 1065 2004 4695 513 Size: 552 939 2691 4048 Fragments arranged by size: 4048 2691 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3989 487 Size: 683 56 2763 4728 Fragments arranged by size: 4728 2763 683 56 BtsI GCAGTG_nn' Cuts at: 2314 3235 4873 5147 5746 6540 6560 2314 Size: 921 1638 274 599 794 20 3984 Fragments arranged by size: 3984 1638 921 794 599 274 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8230 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8195 DraI TTT'AAA Cuts at: 4800 4830 5534 4800 Size: 30 704 7496 Fragments arranged by size: 7496 704 30 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4307 7237 809 Size: 507 2991 2930 1802 Fragments arranged by size: 2991 2930 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 4351 5677 Size: 54 1425 50 275 18 177 2344 1326 Cuts at: 5677 6566 8195 8 Size: 889 1629 43 Fragments arranged by size: 2344 1629 1425 1326 889 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5677 1537 Size: 275 3865 4090 Fragments arranged by size: 4090 3865 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2123 3492 4260 6160 1353 Size: 770 1369 768 1900 3423 Fragments arranged by size: 3423 1900 1369 770 768 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2305 6817 7191 7337 387 Size: 1064 138 716 4512 374 146 1280 Fragments arranged by size: 4512 1280 1064 716 374 146 138 EcoICRI GAG'CTC Cuts at: 4681 4681 Size: 8230 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2395 3426 3474 6293 6890 7677 7945 Size: 393 763 1031 48 2819 597 787 268 Cuts at: 7945 1239 Size: 1524 Fragments arranged by size: 2819 1524 1031 787 763 597 393 268 48 EcoNI CCTnn'n_nnAGG Cuts at: 3158 4943 3158 Size: 1785 6445 EcoO109I rG'GnC_Cy Cuts at: 3616 3616 Size: 8230 EcoRI G'AATT_C Cuts at: 3542 4214 5438 5539 5917 3542 Size: 672 1224 101 378 5855 Fragments arranged by size: 5855 1224 672 378 101 EcoRV GAT'ATC Cuts at: 2888 3151 4391 4495 2888 Size: 263 1240 104 6623 Fragments arranged by size: 6623 1240 263 104 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2566 4804 5441 2566 Size: 2238 637 5355 Fragments arranged by size: 5355 2238 637 FspI TGC'GCA Cuts at: 6736 6736 Size: 8230 HaeII r_GCGC'y Cuts at: 1678 7377 1678 Size: 5699 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2075 2107 2641 2673 3036 Size: 32 135 32 503 32 534 32 363 Cuts at: 3036 3068 3873 3905 4058 4090 4493 4525 5011 Size: 32 805 32 153 32 403 32 486 Cuts at: 5011 5018 5043 5050 6871 6903 6945 6977 1373 Size: 7 25 7 1821 32 42 32 2626 Fragments arranged by size: 2626 1821 805 534 503 486 403 363 153 135 42 32 32 32 32 32 32 32 32 32 32 25 7 7 HincII GTy'rAC Cuts at: 669 1657 1938 4888 4923 5574 5820 669 Size: 988 281 2950 35 651 246 3079 Fragments arranged by size: 3079 2950 988 651 281 246 35 HindIII A'AGCT_T Cuts at: 748 3429 748 Size: 2681 5549 HpaI GTT'AAC Cuts at: 5820 5820 Size: 8230 KpnI G_GTAC'C Cuts at: 4024 5555 4024 Size: 1531 6699 MfeI C'AATT_G Cuts at: 3828 3980 5829 3828 Size: 152 1849 6229 Fragments arranged by size: 6229 1849 152 MluI A'CGCG_T Cuts at: 8106 8106 Size: 8230 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2242 7344 7528 1305 Size: 937 5102 184 2007 Fragments arranged by size: 5102 2007 937 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 4353 10 Size: 54 1425 343 177 2344 3887 Fragments arranged by size: 3887 2344 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2822 4232 6188 6547 6706 518 Size: 648 1656 1410 1956 359 159 2042 Fragments arranged by size: 2042 1956 1656 1410 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3424 5197 5646 6323 7471 7716 1606 Size: 1818 1773 449 677 1148 245 2120 Fragments arranged by size: 2120 1818 1773 1148 677 449 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6739 Fragments arranged by size: 6739 939 552 NdeI CA'TA_TG Cuts at: 387 4471 387 Size: 4084 4146 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8230 NotI GC'GGCC_GC Cuts at: 5677 5677 Size: 8230 NspI r_CATG'y Cuts at: 1132 1229 2442 3781 3827 3910 4844 5588 7133 Size: 97 1213 1339 46 83 934 744 1545 Cuts at: 7133 1132 Size: 2229 Fragments arranged by size: 2229 1545 1339 1213 934 744 97 83 46 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8230 PciI A'CATG_T Cuts at: 3777 7129 3777 Size: 3352 4878 PflMI CCAn_nnn'nTGG Cuts at: 1108 2947 2959 1108 Size: 1839 12 6379 Fragments arranged by size: 6379 1839 12 PmeI GTTT'AAAC Cuts at: 5534 5534 Size: 8230 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6299 6331 7812 7844 6299 Size: 32 1481 32 6685 Fragments arranged by size: 6685 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3579 1613 Size: 1966 6264 PsiI TTA'TAA Cuts at: 3660 3774 3846 5800 3660 Size: 114 72 1954 6090 Fragments arranged by size: 6090 1954 114 72 PstI C_TGCA'G Cuts at: 830 2309 3242 5582 830 Size: 1479 933 2340 3478 Fragments arranged by size: 3478 2340 1479 933 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8230 PvuII CAG'CTG Cuts at: 3424 5197 3424 Size: 1773 6457 SacI G_AGCT'C Cuts at: 4683 4683 Size: 8230 SalI G'TCGA_C Cuts at: 1936 4921 5572 1936 Size: 2985 651 4594 Fragments arranged by size: 4594 2985 651 SbfI CC_TGCA'GG Cuts at: 5582 5582 Size: 8230 ScaI AGT'ACT Cuts at: 6478 8190 6478 Size: 1712 6518 SfcI C'TryA_G Cuts at: 826 1045 2305 3238 3627 5578 6713 7394 7585 Size: 219 1260 933 389 1951 1135 681 191 Cuts at: 7585 826 Size: 1471 Fragments arranged by size: 1951 1471 1260 1135 933 681 389 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8230 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8230 SmaI CCC'GGG Cuts at: 1872 5557 1872 Size: 3685 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2444 2561 4312 4749 6334 7235 Size: 197 926 501 117 1751 437 1585 901 Cuts at: 7235 7497 820 Size: 262 1553 Fragments arranged by size: 1751 1585 1553 926 901 501 437 262 197 117 SnaBI TAC'GTA Cuts at: 493 4168 4208 6021 493 Size: 3675 40 1813 2702 Fragments arranged by size: 3675 2702 1813 40 SpeI A'CTAG_T Cuts at: 152 4580 152 Size: 4428 3802 SphI G_CATG'C Cuts at: 1132 5588 1132 Size: 4456 3774 SspI AAT'ATT Cuts at: 5 52 3272 3766 4456 6154 5 Size: 47 3220 494 690 1698 2081 Fragments arranged by size: 3220 2081 1698 690 494 47 StuI AGG'CCT Cuts at: 1517 2388 2859 1517 Size: 871 471 6888 Fragments arranged by size: 6888 871 471 StyI C'CwwG_G Cuts at: 513 1065 2004 3876 5669 513 Size: 552 939 1872 1793 3074 Fragments arranged by size: 3074 1872 1793 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2199 3791 6257 6274 6427 6612 7057 Size: 1255 619 1592 2466 17 153 185 445 Cuts at: 7057 325 Size: 1498 Fragments arranged by size: 2466 1592 1498 1255 619 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3039 3143 3255 Size: 274 80 33 51 1443 1062 104 112 Cuts at: 3255 6476 8188 96 Size: 3221 1712 138 Fragments arranged by size: 3221 1712 1443 1062 274 138 112 104 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2284 2316 4482 4514 8190 8222 2284 Size: 32 2166 32 3676 32 2292 Fragments arranged by size: 3676 2292 2166 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8230 XbaI T'CTAG_A Cuts at: 5434 5566 5434 Size: 132 8098 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4702 4702 Size: 8230 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8230 XmaI C'CCGG_G Cuts at: 1870 5555 1870 Size: 3685 4545 XmnI GAAnn'nnTTC Cuts at: 6359 6359 Size: 8230 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6038 276 Size: 53 83 186 936 4504 2468 Fragments arranged by size: 4504 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI