Restriction map for
pFN21AB3540
(Linear) (Six-base) MAPSORT of: KIBB3540.seq Check: 558 from: 1 to: 3525
KIBB3540.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AccI GT'mk_AC
Cuts at: 0 716 2917 3525
Size: 716 2201 608
Fragments arranged by size:
2201 716 608
Acc65I G'GTAC_C
Cuts at: 0 2015 3525
Size: 2015 1510
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 390 1421 3525
Size: 390 1031 2104
Fragments arranged by size:
2104 1031 390
AflIII A'CryG_T
Cuts at: 0 1772 3525
Size: 1772 1753
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1357 2121 3241 3525
Size: 1357 764 1120 284
Fragments arranged by size:
1357 1120 764 284
AlwNI CAG_nnn'CTG
Cuts at: 0 3012 3192 3525
Size: 3012 180 333
Fragments arranged by size:
3012 333 180
ApoI r'AATT_y
Cuts at: 0 16 488 770 1537 1543 1828 1990 2209
Size: 16 472 282 767 6 285 162 219
Cuts at: 2209 2518 2817 3002 3433 3525
Size: 309 299 185 431 92
Fragments arranged by size:
767 472 431 309 299 285 282 219
185 162 92 16 6
BamHI G'GATC_C
Cuts at: 0 225 2354 3525
Size: 225 2129 1171
Fragments arranged by size:
2129 1171 225
BanI G'GyrC_C
Cuts at: 0 2015 2155 3525
Size: 2015 140 1370
Fragments arranged by size:
2015 1370 140
BanII G_rGCy'C
Cuts at: 0 602 2678 3525
Size: 602 2076 847
Fragments arranged by size:
2076 847 602
BbsI GAAGACnn'nnnn_
Cuts at: 0 562 903 1013 1106 1769 1819 2765 3525
Size: 562 341 110 93 663 50 946 760
Fragments arranged by size:
946 760 663 562 341 110 93 50
BbvCI CC'TCA_GC
Cuts at: 0 3098 3127 3525
Size: 3098 29 398
Fragments arranged by size:
3098 398 29
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 990 1024 1840 1874 3450 3484 3525
Size: 990 34 816 34 1576 34 41
Fragments arranged by size:
1576 990 816 41 34 34 34
BclI T'GATC_A
Cuts at: 0 1703 3080 3525
Size: 1703 1377 445
Fragments arranged by size:
1703 1377 445
BglI GCCn_nnn'nGGC
Cuts at: 0 799 3525
Size: 799 2726
BglII A'GATC_T
Cuts at: 0 312 669 1275 3525
Size: 312 357 606 2250
Fragments arranged by size:
2250 606 357 312
Bme1580I G_kGCm'C
Cuts at: 0 2597 3525
Size: 2597 928
BmgBI CAC'GTC
Cuts at: 0 2592 3525
Size: 2592 933
BmrI ACTGGGnnnn_n'
Cuts at: 0 569 901 2591 3525
Size: 569 332 1690 934
Fragments arranged by size:
1690 934 569 332
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1469 3525
Size: 1469 2056
Bpu10I CC'TnA_GC
Cuts at: 0 3098 3127 3525
Size: 3098 29 398
Fragments arranged by size:
3098 398 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 541 2328 2729 3525
Size: 424 117 1787 401 796
Fragments arranged by size:
1787 796 424 401 117
BsaI GGTCTCn'nnnn_
Cuts at: 0 3110 3525
Size: 3110 415
BsaAI yAC'GTr
Cuts at: 0 2163 2203 3525
Size: 2163 40 1322
Fragments arranged by size:
2163 1322 40
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1031 1061 1868 1898 2477 2507 3525
Size: 1031 30 807 30 579 30 1018
Fragments arranged by size:
1031 1018 807 579 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 94 1510 1534 3115 3525
Size: 94 1416 24 1581 410
Fragments arranged by size:
1581 1416 410 94 24
BseYI C'CCAG_C
Cuts at: 0 1082 1464 1715 3185 3525
Size: 1082 382 251 1470 340
Fragments arranged by size:
1470 1082 382 340 251
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2134 2856 3106 3525
Size: 2134 722 250 419
Fragments arranged by size:
2134 722 419 250
BsiEI CG_ry'CG
Cuts at: 0 208 3525
Size: 208 3317
BsiHKAI G_wGCw'C
Cuts at: 0 2678 3234 3525
Size: 2678 556 291
Fragments arranged by size:
2678 556 291
BsmI GAATG_Cn'
Cuts at: 0 187 3525
Size: 187 3338
Bsp1286I G_dGCh'C
Cuts at: 0 602 2597 2678 3234 3525
Size: 602 1995 81 556 291
Fragments arranged by size:
1995 602 556 291 81
BspHI T'CATG_A
Cuts at: 0 2036 2922 3525
Size: 2036 886 603
Fragments arranged by size:
2036 886 603
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2106 3128 3525
Size: 2106 1022 397
Fragments arranged by size:
2106 1022 397
BsrDI GCAATG_nn'
Cuts at: 0 376 809 1139 2951 3525
Size: 376 433 330 1812 574
Fragments arranged by size:
1812 574 433 376 330
BsrGI T'GTAC_A
Cuts at: 0 1034 1138 3525
Size: 1034 104 2387
Fragments arranged by size:
2387 1034 104
BssSI C'ACGA_G
Cuts at: 0 3172 3525
Size: 3172 353
BstBI TT'CG_AA
Cuts at: 0 2403 3525
Size: 2403 1122
BstXI CCAn_nnnn'nTGG
Cuts at: 0 237 3396 3525
Size: 237 3159 129
Fragments arranged by size:
3159 237 129
BstYI r'GATC_y
Cuts at: 0 225 312 669 753 1275 1287 2354 2508
Size: 225 87 357 84 522 12 1067 154
Cuts at: 2508 3525
Size: 1017
Fragments arranged by size:
1067 1017 522 357 225 154 87 84
12
BtgI C'CryG_G
Cuts at: 0 2690 3525
Size: 2690 835
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1984 3525
Size: 1984 1541
BtsI GCAGTG_nn'
Cuts at: 0 309 1230 2868 3142 3525
Size: 309 921 1638 274 383
Fragments arranged by size:
1638 921 383 309 274
DraI TTT'AAA
Cuts at: 0 2795 2825 3525
Size: 2795 30 700
Fragments arranged by size:
2795 700 30
DrdI GACnn_nn'nnGTC
Cuts at: 0 2302 3525
Size: 2302 1223
EaeI y'GGCC_r
Cuts at: 0 2 2346 3525
Size: 2 2344 1179
Fragments arranged by size:
2344 1179 2
EarI CTCTTCn'nnn_
Cuts at: 0 118 1487 2255 3525
Size: 118 1369 768 1270
Fragments arranged by size:
1369 1270 768 118
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 300 3525
Size: 300 3225
EcoICRI GAG'CTC
Cuts at: 0 2676 3525
Size: 2676 849
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 390 1421 1469 3525
Size: 390 1031 48 2056
Fragments arranged by size:
2056 1031 390 48
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1153 2938 3525
Size: 1153 1785 587
Fragments arranged by size:
1785 1153 587
EcoO109I rG'GnC_Cy
Cuts at: 0 1611 3525
Size: 1611 1914
EcoRI G'AATT_C
Cuts at: 0 1537 2209 3433 3525
Size: 1537 672 1224 92
Fragments arranged by size:
1537 1224 672 92
EcoRV GAT'ATC
Cuts at: 0 883 1146 2386 2490 3525
Size: 883 263 1240 104 1035
Fragments arranged by size:
1240 1035 883 263 104
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 561 2799 3436 3525
Size: 561 2238 637 89
Fragments arranged by size:
2238 637 561 89
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 70 102 636 668 1031 1063 1868 1900
Size: 70 32 534 32 363 32 805 32
Cuts at: 1900 2053 2085 2488 2520 3006 3013 3038 3045
Size: 153 32 403 32 486 7 25 7
Cuts at: 3045 3525
Size: 480
Fragments arranged by size:
805 534 486 480 403 363 153 70
32 32 32 32 32 32 25 7
7
HincII GTy'rAC
Cuts at: 0 2883 2918 3525
Size: 2883 35 607
Fragments arranged by size:
2883 607 35
HindIII A'AGCT_T
Cuts at: 0 1424 3525
Size: 1424 2101
KpnI G_GTAC'C
Cuts at: 0 2019 3525
Size: 2019 1506
MfeI C'AATT_G
Cuts at: 0 1823 1975 3525
Size: 1823 152 1550
Fragments arranged by size:
1823 1550 152
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 237 3525
Size: 237 3288
MscI TGG'CCA
Cuts at: 0 4 2348 3525
Size: 4 2344 1177
Fragments arranged by size:
2344 1177 4
MslI CAynn'nnrTG
Cuts at: 0 817 2227 3525
Size: 817 1410 1298
Fragments arranged by size:
1410 1298 817
MspA1I CmG'CkG
Cuts at: 0 1419 3192 3525
Size: 1419 1773 333
Fragments arranged by size:
1773 1419 333
NdeI CA'TA_TG
Cuts at: 0 2466 3525
Size: 2466 1059
NspI r_CATG'y
Cuts at: 0 437 1776 1822 1905 2839 3525
Size: 437 1339 46 83 934 686
Fragments arranged by size:
1339 934 686 437 83 46
PciI A'CATG_T
Cuts at: 0 1772 3525
Size: 1772 1753
PflMI CCAn_nnn'nTGG
Cuts at: 0 942 954 3525
Size: 942 12 2571
Fragments arranged by size:
2571 942 12
PshAI GACnn'nnGTC
Cuts at: 0 1574 3525
Size: 1574 1951
PsiI TTA'TAA
Cuts at: 0 1655 1769 1841 3525
Size: 1655 114 72 1684
Fragments arranged by size:
1684 1655 114 72
PstI C_TGCA'G
Cuts at: 0 304 1237 3525
Size: 304 933 2288
Fragments arranged by size:
2288 933 304
PvuII CAG'CTG
Cuts at: 0 1419 3192 3525
Size: 1419 1773 333
Fragments arranged by size:
1773 1419 333
SacI G_AGCT'C
Cuts at: 0 2678 3525
Size: 2678 847
SalI G'TCGA_C
Cuts at: 0 2916 3525
Size: 2916 609
SfcI C'TryA_G
Cuts at: 0 300 1233 1622 3525
Size: 300 933 389 1903
Fragments arranged by size:
1903 933 389 300
SmlI C'TyrA_G
Cuts at: 0 439 556 2307 2744 3525
Size: 439 117 1751 437 781
Fragments arranged by size:
1751 781 439 437 117
SnaBI TAC'GTA
Cuts at: 0 2163 2203 3525
Size: 2163 40 1322
Fragments arranged by size:
2163 1322 40
SpeI A'CTAG_T
Cuts at: 0 2575 3525
Size: 2575 950
SspI AAT'ATT
Cuts at: 0 1267 1761 2451 3525
Size: 1267 494 690 1074
Fragments arranged by size:
1267 1074 690 494
StuI AGG'CCT
Cuts at: 0 383 854 3525
Size: 383 471 2671
Fragments arranged by size:
2671 471 383
StyI C'CwwG_G
Cuts at: 0 1871 3525
Size: 1871 1654
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 194 1786 3525
Size: 194 1592 1739
Fragments arranged by size:
1739 1592 194
TatI w'GTAC_w
Cuts at: 0 1034 1138 1250 3525
Size: 1034 104 112 2275
Fragments arranged by size:
2275 1034 112 104
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 279 311 2477 2509 3525
Size: 279 32 2166 32 1016
Fragments arranged by size:
2166 1016 279 32 32
XbaI T'CTAG_A
Cuts at: 0 3429 3525
Size: 3429 96
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2697 3525
Size: 2697 828
Enzymes that do cut:
AccI Acc65I AcuI AflIII AlfI AlwNI ApoI BamHI
BanI BanII BbsI BbvCI BcgI BclI BglI BglII
Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrDI BsrGI BssSI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI Hin4I
HincII HindIII KpnI MfeI MmeI MscI MslI MspA1I
NdeI NspI PciI PflMI PshAI PsiI PstI PvuII
SacI SalI SfcI SmlI SnaBI SpeI SspI StuI
StyI TaqII TatI TstI XbaI XcmI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AleI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BbeI BciVI BfrBI BlpI BmtI BplI BsaBI
BsaHI BsaWI BsiWI BsmBI BspEI BsrBI BsrFI BssHII
BstAPI BstEII BstZ17I Bsu36I ClaI CspCI DraIII EagI
FseI FspI FspAI HaeII HpaI KasI MluI NaeI
NarI NcoI NgoMIV NheI NotI NruI NsiI PacI
PasI PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI
PsrI PvuI RsrII SacII SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SphI SrfI
SwaI Tth111I XhoI XmaI XmnI ZraI