Restriction map for
pFN21AB3476
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# NO CUT ENZYMES ( 127 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI AspEI AspI
AviII AvrII BalI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BfrI BlnI BlpI Bpu1102I BpuAI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsmBI BsmI BspDI BspEI
BspMI BsrFI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstUI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoT22I
FauI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI KasI KspI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NdeI NgoMIV NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SpeI
SphI Sse8387I SspI SwaI Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 35 enzymes) #
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Acc65I 1661 GGTACC G^GTACC
Asp700I 1182 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1661 GGTACC G^GTACC
BamHI 1956 GGATCC G^GATCC
BanI 1661 GGTACC G^G[CT][GA]CC
BclI 1260 TGATCA T^GATCA
BglII 774 AGATCT A^GATCT
BsiWI 1450 CGTACG C^GTACG
Bsp1407I 1966 TGTACA T^GTACA
BspHI 1005 TCATGA T^CATGA
BsrBI 2116 GAGCGG CCGCTC(-3/-3)
BsrGI 1966 TGTACA T^GTACA
BstBI 1985 TTCGAA TT^CGAA
CpoI 525 CGGTCCG CG^G[AT]CCG
DrdI 2074 GACAATAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1072 GAATTC G^AATTC
EcoRV 1522 GATATC GAT^ATC
FbaI 1260 TGATCA T^GATCA
HindIII 641 AAGCTT A^AGCTT
KpnI 1661 GGTACC GGTAC^C
MspA1I 1684 CAGCTG C[AC]G^C[GT]G
NheI 1299 GCTAGC G^CTAGC
NspV 1985 TTCGAA TT^CGAA
PvuII 1684 CAGCTG CAG^CTG
RcaI 1005 TCATGA T^CATGA
RsrII 525 CGGTCCG CG^G[AT]CCG
SfuI 1985 TTCGAA TT^CGAA
SmaI 1358 CCCGGG CCC^GGG
SnaBI 1448 TACGTA TAC^GTA
SspBI 1966 TGTACA T^GTACA
StuI 333 AGGCCT AGG^CCT
XcmI 882 CCAGAGAATTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1358 CCCGGG C^CCGGG
XmaI 1358 CCCGGG C^CCGGG
XmnI 1182 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
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AspHI 470 GTGCTC G[AT]GC[AT]^C
1340 GAGCAC
AvaI 1155 CTCGGG C^[CT]CG[GA]G
1358 CCCGGG
BglI 1290 GCCTCTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1836 GCCCTTTTGGC
BmyI 470 GTGCTC G[AGT]GC[ACT]^C
1340 GAGCAC
BsaAI 846 CACGTA [CT]AC^GT[GA]
1448 TACGTA
BsaBI 1800 GATTGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1951 GATCTGGATC
BseRI 7 GAGGAG GAGGAG(10/8)
46 GAGGAG
BsiHKAI 470 GTGCTC G[AT]GC[AT]^C
1340 GAGCAC
BsmAI 874 GAGAC GTCTC(1/5)
2072 GAGAC
BsoBI 1155 CTCGGG C^[CT]CG[GA]G
1358 CCCGGG
Bsp1286I 470 GTGCTC G[AGT]GC[ACT]^C
1340 GAGCAC
BspLU11I 557 ACATGT A^CATGT
1184 ACATGT
BsrDI 587 GCAATG GCAATG(2/0)
682 GCAATG
DraI 822 TTTAAA TTT^AAA
1480 TTTAAA
EaeI 338 TGGCCG [CT]^GGCC[GA]
1267 CGGCCA
EarI 597 CTCTTC CTCTTC(1/4)
70 GAAGAG
Ksp632I 597 CTCTTC CTCTTC(1/4)
70 GAAGAG
MaeIII 253 GTCAC ^GT[ACGT]AC
1117 GTCAC
MamI 1800 GATTGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1951 GATCTGGATC
NcoI 491 CCATGG C^CATGG
1403 CCATGG
NspI 557 ACATGT [GA]CATG^[CT]
1184 ACATGT
PflMI 845 CCACGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1857 CCAGGTAATGG
SmlI 172 CTTGAG C^T[CT][GA]AG
424 CTCAAG
Tsp45I 253 GTCAC ^GT[GC]AC
1117 GTCAC
Van91I 845 CCACGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1857 CCAGGTAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AvaII BbvI BcnI
BfaI BpmI BsaJI BsiYI BslI
BsmFI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII DraII DsaI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeI
MaeII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
XhoII