Restriction map for
pFN21AB3435
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspHI AspI AviII AvrII
BamHI BbrPI BcgI BclI BfrI
BlnI BsaAI BsaBI BseAI BsiHKAI
BsiWI BsmI Bsp1407I BspEI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstUI BstXI
BstZ17I Bsu36I Cfr10I CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI EcoRV
FauI FbaI FseI FspI HindIII
HpaI KspI MamI MfeI MluI
MroI MspA1I MunI MvnI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tth111I XbaI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 469 GGTACC G^GTACC
AlwI 23 GATCC GGATC(4/5)
AlwNI 433 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 469 GGTACC G^GTACC
BalI 995 TGGCCA TGG^CCA
BanII 52 GAGCCC G[GA]GC[CT]^C
BbeI 382 GGCGCC GGCGC^C
BbsI 1135 GAAGAC GAAGAC(2/6)
BbvCI 424 GCTGAGG CCTCAGC(-5/-2)
BcnI 842 CCCGG CC^[GC]GG
BglI 298 GCCACCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 337 GCTCAGC GC^T[ACGT]AGC
BpmI 727 CTGGAG CTGGAG(16/14)
Bpu1102I 337 GCTCAGC GC^T[ACGT]AGC
BpuAI 1135 GAAGAC GAAGAC(2/6)
BsaI 1162 GAGACC GGTCTC(1/5)
BsaWI 500 ACCGGA [AT]^CCGG[AT]
BseRI 766 GAGGAG GAGGAG(10/8)
BsiEI 1080 CGGTCG CG[GA][CT]^CG
BsmBI 932 GAGACG CGTCTC(1/5)
BspDI 264 ATCGAT AT^CGAT
BspHI 680 TCATGA T^CATGA
BstAPI 432 GCAGAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 666 GGTGACC G^GT[ACGT]ACC
BstPI 666 GGTGACC G^GT[ACGT]ACC
CelII 337 GCTCAGC GC^T[ACGT]AGC
CfoI 383 GCGC GCG^C
ClaI 264 ATCGAT AT^CGAT
DraII 686 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 785 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 686 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 666 GGTGACC G^GT[ACGT]ACC
EcoT14I 308 CCTTGG C^C[AT][AT]GG
HaeII 382 GGCGCC [GA]GCGC^[CT]
HgaI 62 GCGTC GACGC(5/10)
HhaI 383 GCGC GCG^C
HinP1I 383 GCGC G^CGC
KasI 382 GGCGCC G^GCGCC
KpnI 469 GGTACC GGTAC^C
MluNI 995 TGGCCA TGG^CCA
MscI 995 TGGCCA TGG^CCA
NarI 382 GGCGCC GG^CGCC
NciI 842 CCCGG CC^[GC]GG
NspI 876 GCATGC [GA]CATG^[CT]
PflMI 303 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 686 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 680 TCATGA T^CATGA
SfoI 382 GGCGCC GGC^GCC
SphI 876 GCATGC GCATG^C
StuI 305 AGGCCT AGG^CCT
StyI 308 CCTTGG C^C[AT][AT]GG
Van91I 303 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AciI 738 CCGC CCGC(-3/-1)
899 CCGC
AcyI 61 GGCGTC G[GA]^CG[CT]C
382 GGCGCC
AfaI 470 GTAC GT^AC
538 GTAC
AvaI 326 CTCGAG C^[CT]CG[GA]G
881 CTCGAG
BciVI 734 GTATCC GTATCC(6/5)
895 GTATCC
BglII 288 AGATCT A^GATCT
584 AGATCT
BmyI 52 GAGCCC G[AGT]GC[ACT]^C
408 GGGCAC
BsaHI 61 GGCGTC G[GA]^CG[CT]C
382 GGCGCC
BsgI 430 GTGCAG GTGCAG(16/14)
1012 GTGCAG
BsoBI 326 CTCGAG C^[CT]CG[GA]G
881 CTCGAG
Bsp1286I 52 GAGCCC G[AGT]GC[ACT]^C
408 GGGCAC
BspMI 989 ACCTGC ACCTGC(4/8)
1017 GCAGGT
BstYI 288 AGATCT [GA]^GATC[CT]
584 AGATCT
DdeI 338 CTCAG C^T[ACGT]AG
425 CTGAG
DsaI 132 CCGTGG C^C[GA][CT]GG
567 CCGTGG
EarI 1148 CTCTTC CTCTTC(1/4)
715 GAAGAG
EcoT22I 874 ATGCAT ATGCA^T
904 ATGCAT
HapII 501 CCGG C^CGG
843 CCGG
Hin1I 61 GGCGTC G[GA]^CG[CT]C
382 GGCGCC
HincII 91 GTTGAC GT[CT]^[GA]AC
720 GTTGAC
HindII 91 GTTGAC GT[CT]^[GA]AC
720 GTTGAC
HpaII 501 CCGG C^CGG
843 CCGG
Ksp632I 1148 CTCTTC CTCTTC(1/4)
715 GAAGAG
MflI 288 AGATCT [GA]^GATC[CT]
584 AGATCT
MspI 501 CCGG C^CGG
843 CCGG
NsiI 874 ATGCAT ATGCA^T
904 ATGCAT
PaeR7I 326 CTCGAG C^TCGAG
881 CTCGAG
PleI 1146 GACTC GAGTC(4/5)
121 GAGTC
PstI 271 CTGCAG CTGCA^G
439 CTGCAG
RsaI 470 GTAC GT^AC
538 GTAC
SfaNI 873 GATGC GCATC(5/9)
137 GCATC
SfcI 271 CTGCAG C^T[GA][CT]AG
439 CTGCAG
Tsp509I 284 AATT ^AATT
332 AATT
TspRI 891 CACTG CA[GC]TG[ACGT][ACGT]^
984 CACTG
XhoI 326 CTCGAG C^TCGAG
881 CTCGAG
XhoII 288 AGATCT [GA]^GATC[CT]
584 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AluI AvaII BanI BbvI BfaI
BsaJI BsiYI BslI BsmAI BsmFI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DpnII EaeI EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SmlI TaqI TfiI Tru9I
TseI Tsp45I TthHB8I XcmI