(Circular) (Six-base) MAPSORT of: pFN21AB3435.seq Check: 1370 from: 1 to: 5923 pFN21AB3435 5923 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3733 278 Size: 53 83 186 936 2197 2468 Fragments arranged by size: 2468 2197 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3266 1937 Size: 1329 4594 Acc65I G'GTAC_C Cuts at: 2474 3244 2474 Size: 770 5153 AclI AA'CG_TT Cuts at: 1563 4050 4423 1563 Size: 2487 373 3063 Fragments arranged by size: 3063 2487 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3986 5370 5638 1632 Size: 2354 1384 268 1917 Fragments arranged by size: 2354 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5726 AflIII A'CryG_T Cuts at: 1203 4822 5799 1203 Size: 3619 977 1327 Fragments arranged by size: 3619 1327 977 AgeI A'CCGG_T Cuts at: 4754 4754 Size: 5923 AhdI GACnn_n'nnGTC Cuts at: 4652 4652 Size: 5923 AleI CACnn'nnGTG Cuts at: 1166 2420 1166 Size: 1254 4669 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2438 3032 1184 Size: 1254 594 4075 Fragments arranged by size: 4075 1254 594 AlwNI CAG_nnn'CTG Cuts at: 2443 3666 5238 2443 Size: 1223 1572 3128 Fragments arranged by size: 3128 1572 1223 ApaLI G'TGCA_C Cuts at: 3980 5136 3980 Size: 1156 4767 ApoI r'AATT_y Cuts at: 1493 3232 3458 3610 5719 1493 Size: 1739 226 152 2109 1697 Fragments arranged by size: 2109 1739 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4477 160 Size: 872 3445 1606 Fragments arranged by size: 3445 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 AvaI C'yCGr_G Cuts at: 1870 1943 2331 2886 3248 1870 Size: 73 388 555 362 4545 Fragments arranged by size: 4545 555 388 362 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2389 2422 2389 Size: 33 5890 BamHI G'GATC_C Cuts at: 3253 3620 3253 Size: 367 5556 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2387 2414 2474 3244 4699 Size: 325 217 627 600 27 60 770 1455 Cuts at: 4699 618 Size: 1842 Fragments arranged by size: 1842 1455 770 627 600 325 217 60 27 BanII G_rGCy'C Cuts at: 1391 2061 3242 1391 Size: 670 1181 4072 Fragments arranged by size: 4072 1181 670 BbeI G_GCGC'C Cuts at: 2391 2391 Size: 5923 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3147 928 Size: 971 1248 3704 Fragments arranged by size: 3704 1248 971 BbvCI CC'TCA_GC Cuts at: 2430 2430 Size: 5923 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4114 4148 1903 Size: 34 2177 34 3678 Fragments arranged by size: 3678 2177 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2750 2911 3815 5025 2750 Size: 161 904 1210 3648 Fragments arranged by size: 3648 1210 904 161 BclI T'GATC_A Cuts at: 1548 1548 Size: 5923 BfrBI ATG'CAT Cuts at: 2881 2911 2881 Size: 30 5893 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2309 4534 136 Size: 107 122 71 631 605 637 2225 1525 Fragments arranged by size: 2225 1525 637 631 605 122 107 71 BglII A'GATC_T Cuts at: 2293 2589 5918 2293 Size: 296 3329 2298 Fragments arranged by size: 3329 2298 296 BlpI GC'TnA_GC Cuts at: 2343 3340 2343 Size: 997 4926 Bme1580I G_kGCm'C Cuts at: 1790 2417 3984 5140 1790 Size: 627 1567 1156 2573 Fragments arranged by size: 2573 1567 1156 627 BmgBI CAC'GTC Cuts at: 1318 2941 1318 Size: 1623 4300 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2253 3654 4612 Size: 416 53 83 399 24 836 1401 958 Cuts at: 4612 442 Size: 1753 Fragments arranged by size: 1753 1401 958 836 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5923 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2185 2185 Size: 5923 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2753 4583 1239 Size: 1514 1830 2579 Fragments arranged by size: 2579 1830 1514 Bpu10I CC'TnA_GC Cuts at: 2430 2430 Size: 5923 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2211 2687 4048 4913 5211 2211 Size: 476 1361 865 298 2923 Fragments arranged by size: 2923 1361 865 476 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3161 4586 882 Size: 617 1662 1425 2219 Fragments arranged by size: 2219 1662 1425 617 BsaAI yAC'GTr Cuts at: 493 1204 3714 493 Size: 711 2510 2702 Fragments arranged by size: 2702 2510 711 BsaBI GATnn'nnATC Cuts at: 5917 5917 Size: 5923 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2067 2388 3730 4112 Size: 53 83 186 936 534 321 1342 382 Cuts at: 4112 4747 275 Size: 635 1451 Fragments arranged by size: 1451 1342 936 635 534 382 321 186 83 53 BsaWI w'CCGG_w Cuts at: 2505 4356 4754 5028 5175 5586 2505 Size: 1851 398 274 147 411 2842 Fragments arranged by size: 2842 1851 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3167 3197 1178 Size: 30 165 30 1764 30 3904 Fragments arranged by size: 3904 1764 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2786 1186 Size: 1600 4323 BseYI C'CCAG_C Cuts at: 5126 5622 5126 Size: 496 5427 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2456 3038 1163 Size: 573 720 582 4048 Fragments arranged by size: 4048 720 582 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3088 3373 4134 5162 1540 Size: 275 186 1087 285 761 1028 2301 Fragments arranged by size: 2301 1087 1028 761 285 275 186 BsiHKAI G_wGCw'C Cuts at: 3984 4069 5140 3984 Size: 85 1071 4767 Fragments arranged by size: 4767 1071 85 BsmI GAATG_Cn' Cuts at: 3434 3527 3434 Size: 93 5830 BsmBI CGTCTCn'nnnn_ Cuts at: 2931 2931 Size: 5923 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2061 2417 3242 3984 4069 5140 1391 Size: 399 271 356 825 742 85 1071 2174 Fragments arranged by size: 2174 1071 825 742 399 356 271 85 BspEI T'CCGG_A Cuts at: 5586 5586 Size: 5923 BspHI T'CATG_A Cuts at: 2685 3812 5542 2685 Size: 1127 1730 3066 Fragments arranged by size: 3066 1730 1127 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3003 3013 3278 844 Size: 2159 10 265 3489 Fragments arranged by size: 3489 2159 265 10 BsrBI CCG'CTC Cuts at: 1394 1823 3810 1394 Size: 429 1987 3507 Fragments arranged by size: 3507 1987 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4418 4592 65 Size: 1183 3170 174 1396 Fragments arranged by size: 3170 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4567 4754 1700 Size: 110 2757 187 2869 Fragments arranged by size: 2869 2757 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5923 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5923 BssSI C'ACGA_G Cuts at: 3983 4995 3983 Size: 1012 4911 BstAPI GCAn_nnn'nTGC Cuts at: 794 2443 794 Size: 1649 4274 BstBI TT'CG_AA Cuts at: 4727 4727 Size: 5923 BstEII G'GTnAC_C Cuts at: 1184 2671 1184 Size: 1487 4436 BstYI r'GATC_y Cuts at: 1972 2293 2589 3253 3620 4005 4022 5474 5560 Size: 321 296 664 367 385 17 1452 86 Cuts at: 5560 5572 5918 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 664 385 367 346 321 296 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2137 2572 513 Size: 552 1072 435 3864 Fragments arranged by size: 3864 1072 552 435 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5184 Fragments arranged by size: 5184 683 56 BtsI GCAGTG_nn' Cuts at: 3439 4233 4253 3439 Size: 794 20 5109 Fragments arranged by size: 5109 794 20 ClaI AT'CG_AT Cuts at: 1554 2270 1554 Size: 716 5207 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5888 DraI TTT'AAA Cuts at: 3227 3227 Size: 5923 DraIII CAC_nnn'GTG Cuts at: 2795 2795 Size: 5923 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4930 809 Size: 507 3614 1802 Fragments arranged by size: 3614 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2253 2482 3000 Size: 54 1425 50 275 18 423 229 518 Cuts at: 3000 3103 3370 4259 5888 8 Size: 103 267 889 1629 43 Fragments arranged by size: 1629 1425 889 518 423 275 267 229 103 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3370 1537 Size: 275 1558 4090 Fragments arranged by size: 4090 1558 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2715 3159 3853 1353 Size: 1362 444 694 3423 Fragments arranged by size: 3423 1362 694 444 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2732 2893 4510 4884 5030 387 Size: 1064 138 1143 161 1617 374 146 1280 Fragments arranged by size: 1617 1280 1143 1064 374 161 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2753 3986 4583 5370 5638 1239 Size: 393 1121 1233 597 787 268 1524 Fragments arranged by size: 1524 1233 1121 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2692 2692 Size: 5923 EcoRI G'AATT_C Cuts at: 3232 3610 3232 Size: 378 5545 FspI TGC'GCA Cuts at: 4429 4429 Size: 5923 HaeII r_GCGC'y Cuts at: 1678 2391 5070 1678 Size: 713 2679 2531 Fragments arranged by size: 2679 2531 713 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2012 2044 2440 2472 2869 Size: 32 135 32 440 32 396 32 397 Cuts at: 2869 2901 4564 4596 4638 4670 1373 Size: 32 1663 32 42 32 2626 Fragments arranged by size: 2626 1663 440 397 396 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2098 2727 3267 3513 669 Size: 988 281 160 629 540 246 3079 Fragments arranged by size: 3079 988 629 540 281 246 160 HindIII A'AGCT_T Cuts at: 748 748 Size: 5923 HpaI GTT'AAC Cuts at: 3513 3513 Size: 5923 KasI G'GCGC_C Cuts at: 2387 2387 Size: 5923 KpnI G_GTAC'C Cuts at: 2478 3248 2478 Size: 770 5153 MfeI C'AATT_G Cuts at: 3522 3522 Size: 5923 MluI A'CGCG_T Cuts at: 5799 5799 Size: 5923 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5037 5221 1305 Size: 3732 184 2007 Fragments arranged by size: 3732 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3002 10 Size: 54 1425 343 1170 2931 Fragments arranged by size: 2931 1425 1170 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2420 2468 2534 2759 3014 3881 4240 Size: 648 1254 48 66 225 255 867 359 Cuts at: 4240 4399 518 Size: 159 2042 Fragments arranged by size: 2042 1254 867 648 359 255 225 159 66 48 MspA1I CmG'CkG Cuts at: 1606 3339 4016 5164 5409 1606 Size: 1733 677 1148 245 2120 Fragments arranged by size: 2120 1733 1148 677 245 NarI GG'CG_CC Cuts at: 2388 2388 Size: 5923 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5371 NdeI CA'TA_TG Cuts at: 387 387 Size: 5923 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5923 NotI GC'GGCC_GC Cuts at: 3370 3370 Size: 5923 NsiI A_TGCA'T Cuts at: 2883 2913 2883 Size: 30 5893 NspI r_CATG'y Cuts at: 1132 1229 2885 3281 4826 1132 Size: 97 1656 396 1545 2229 Fragments arranged by size: 2229 1656 1545 396 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5923 PciI A'CATG_T Cuts at: 4822 4822 Size: 5923 PflMI CCAn_nnn'nTGG Cuts at: 1108 2314 1108 Size: 1206 4717 PmeI GTTT'AAAC Cuts at: 3227 3227 Size: 5923 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3992 4024 5505 5537 3992 Size: 32 1481 32 4378 Fragments arranged by size: 4378 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2692 2692 Size: 5923 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5923 PsiI TTA'TAA Cuts at: 3493 3493 Size: 5923 PstI C_TGCA'G Cuts at: 830 2280 2448 3275 830 Size: 1450 168 827 3478 Fragments arranged by size: 3478 1450 827 168 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5923 SalI G'TCGA_C Cuts at: 1936 3265 1936 Size: 1329 4594 SanDI GG'GwC_CC Cuts at: 2692 2692 Size: 5923 SbfI CC_TGCA'GG Cuts at: 3275 3275 Size: 5923 ScaI AGT'ACT Cuts at: 4171 5883 4171 Size: 1712 4211 SfcI C'TryA_G Cuts at: 826 1045 2276 2444 3271 4406 5087 5278 826 Size: 219 1231 168 827 1135 681 191 1471 Fragments arranged by size: 1471 1231 1135 827 681 219 191 168 SfoI GGC'GCC Cuts at: 2389 2389 Size: 5923 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5923 SmaI CCC'GGG Cuts at: 1872 3250 1872 Size: 1378 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2226 2331 2666 2886 4027 4928 Size: 197 926 283 105 335 220 1141 901 Cuts at: 4928 5190 820 Size: 262 1553 Fragments arranged by size: 1553 1141 926 901 335 283 262 220 197 105 SnaBI TAC'GTA Cuts at: 493 3714 493 Size: 3221 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5923 SphI G_CATG'C Cuts at: 1132 2885 3281 1132 Size: 1753 396 3774 Fragments arranged by size: 3774 1753 396 SspI AAT'ATT Cuts at: 5 52 3847 5 Size: 47 3795 2081 Fragments arranged by size: 3795 2081 47 StuI AGG'CCT Cuts at: 1517 2312 1517 Size: 795 5128 StyI C'CwwG_G Cuts at: 513 1065 2313 3362 513 Size: 552 1248 1049 3074 Fragments arranged by size: 3074 1248 1049 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2321 2341 2639 2837 3074 3950 3967 Size: 1255 741 20 298 198 237 876 17 Cuts at: 3967 4120 4305 4750 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 876 741 445 298 237 198 185 153 20 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2542 4169 5881 Size: 274 80 33 51 1443 565 1627 1712 Cuts at: 5881 96 Size: 138 Fragments arranged by size: 1712 1627 1443 565 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5883 5915 5883 Size: 32 5891 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5923 XbaI T'CTAG_A Cuts at: 3259 3259 Size: 5923 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2240 2311 2335 2995 2240 Size: 71 24 660 5168 Fragments arranged by size: 5168 660 71 24 XhoI C'TCGA_G Cuts at: 1943 2331 2886 1943 Size: 388 555 4980 Fragments arranged by size: 4980 555 388 XmaI C'CCGG_G Cuts at: 1870 3248 1870 Size: 1378 4545 XmnI GAAnn'nnTTC Cuts at: 4052 4052 Size: 5923 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3731 276 Size: 53 83 186 936 2197 2468 Fragments arranged by size: 2468 2197 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SrfI SwaI