(Circular) (Six-base) MAPSORT of: pFN21AB3347.seq Check: 895 from: 1 to: 6916 pFN21AB3347 6916 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3249 4726 278 Size: 53 83 186 936 1713 1477 2468 Fragments arranged by size: 2468 1713 1477 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4259 1937 Size: 2322 4594 Acc65I G'GTAC_C Cuts at: 4237 4237 Size: 6916 AclI AA'CG_TT Cuts at: 1563 2123 5043 5416 1563 Size: 560 2920 373 3063 Fragments arranged by size: 3063 2920 560 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2246 2850 3833 3975 4979 6363 6631 1632 Size: 614 604 983 142 1004 1384 268 1917 Fragments arranged by size: 1917 1384 1004 983 614 604 268 142 AflII C'TTAA_G Cuts at: 820 1017 2032 820 Size: 197 1015 5704 Fragments arranged by size: 5704 1015 197 AflIII A'CryG_T Cuts at: 1203 5815 6792 1203 Size: 4612 977 1327 Fragments arranged by size: 4612 1327 977 AgeI A'CCGG_T Cuts at: 5747 5747 Size: 6916 AhdI GACnn_n'nnGTC Cuts at: 5645 5645 Size: 6916 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6916 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6916 AlwNI CAG_nnn'CTG Cuts at: 2096 2674 2731 3070 3229 3394 3978 4659 6231 Size: 578 57 339 159 165 584 681 1572 Cuts at: 6231 2096 Size: 2781 Fragments arranged by size: 2781 1572 681 584 578 339 165 159 57 ApaI G_GGCC'C Cuts at: 2869 2869 Size: 6916 ApaLI G'TGCA_C Cuts at: 4973 6129 4973 Size: 1156 5760 ApoI r'AATT_y Cuts at: 1493 4225 4451 4603 6712 1493 Size: 2732 226 152 2109 1697 Fragments arranged by size: 2732 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5470 160 Size: 872 4438 1606 Fragments arranged by size: 4438 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6916 AvaI C'yCGr_G Cuts at: 1870 1943 2505 2604 2937 4241 1870 Size: 73 562 99 333 1304 4545 Fragments arranged by size: 4545 1304 562 333 99 73 BamHI G'GATC_C Cuts at: 4246 4613 4246 Size: 367 6549 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4237 5692 618 Size: 325 217 627 2450 1455 1842 Fragments arranged by size: 2450 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2331 2869 2938 2944 3491 3654 3997 4235 Size: 940 538 69 6 547 163 343 238 Cuts at: 4235 1391 Size: 4072 Fragments arranged by size: 4072 940 547 538 343 238 163 69 6 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2838 3142 3821 928 Size: 971 939 304 679 4023 Fragments arranged by size: 4023 971 939 679 304 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5107 5141 1903 Size: 34 50 34 3086 34 3678 Fragments arranged by size: 3678 3086 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3556 4808 6018 3556 Size: 1252 1210 4454 Fragments arranged by size: 4454 1252 1210 BclI T'GATC_A Cuts at: 1548 2946 1548 Size: 1398 5518 BfrBI ATG'CAT Cuts at: 3849 3849 Size: 6916 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5527 136 Size: 107 122 71 631 605 3855 1525 Fragments arranged by size: 3855 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6911 6911 Size: 6916 BlpI GC'TnA_GC Cuts at: 4333 4333 Size: 6916 Bme1580I G_kGCm'C Cuts at: 1790 2869 4977 6133 1790 Size: 1079 2108 1156 2573 Fragments arranged by size: 2573 2108 1156 1079 BmgBI CAC'GTC Cuts at: 1318 3435 3923 1318 Size: 2117 488 4311 Fragments arranged by size: 4311 2117 488 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2325 2538 4647 Size: 416 53 83 399 24 908 213 2109 Cuts at: 4647 5605 442 Size: 958 1753 Fragments arranged by size: 2109 1753 958 908 416 399 213 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6916 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2753 3146 5576 1239 Size: 1514 393 2430 2579 Fragments arranged by size: 2579 2430 1514 393 Bpu10I CC'TnA_GC Cuts at: 2109 3602 2109 Size: 1493 5423 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5041 5906 6204 5041 Size: 865 298 5753 Fragments arranged by size: 5753 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2354 3000 3047 5579 882 Size: 617 855 646 47 2532 2219 Fragments arranged by size: 2532 2219 855 646 617 47 BsaAI yAC'GTr Cuts at: 493 1204 4707 493 Size: 711 3503 2702 Fragments arranged by size: 3503 2702 711 BsaBI GATnn'nnATC Cuts at: 2466 4110 6910 2466 Size: 1644 2800 2472 Fragments arranged by size: 2800 2472 1644 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3246 4723 5105 5740 Size: 53 83 186 936 1713 1477 382 635 Cuts at: 5740 275 Size: 1451 Fragments arranged by size: 1713 1477 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3031 5349 5747 6021 6168 6579 3031 Size: 2318 398 274 147 411 3368 Fragments arranged by size: 3368 2318 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6691 Fragments arranged by size: 6691 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2065 2720 3334 1186 Size: 879 655 614 4768 Fragments arranged by size: 4768 879 655 614 BseYI C'CCAG_C Cuts at: 2286 2646 3635 3797 6119 6615 2286 Size: 360 989 162 2322 496 2587 Fragments arranged by size: 2587 2322 989 496 360 162 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2201 3305 3380 1163 Size: 573 465 1104 75 4699 Fragments arranged by size: 4699 1104 573 465 75 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4366 5127 6155 1540 Size: 275 186 2365 761 1028 2301 Fragments arranged by size: 2365 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2487 2938 3502 4977 5062 6133 2487 Size: 451 564 1475 85 1071 3270 Fragments arranged by size: 3270 1475 1071 564 451 85 BsmI GAATG_Cn' Cuts at: 4427 4520 4427 Size: 93 6823 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2331 2487 2869 2938 2944 3491 3502 Size: 399 541 156 382 69 6 547 11 Cuts at: 3502 3654 3997 4235 4977 5062 6133 1391 Size: 152 343 238 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 547 541 399 382 343 238 156 152 85 69 11 6 BspEI T'CCGG_A Cuts at: 6579 6579 Size: 6916 BspHI T'CATG_A Cuts at: 4210 4805 6535 4210 Size: 595 1730 4591 Fragments arranged by size: 4591 1730 595 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4271 844 Size: 3427 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4803 1394 Size: 429 2980 3507 Fragments arranged by size: 3507 2980 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2569 3286 3735 5411 5585 65 Size: 1183 1321 717 449 1676 174 1396 Fragments arranged by size: 1676 1396 1321 1183 717 449 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2037 5560 5747 1700 Size: 110 227 3523 187 2869 Fragments arranged by size: 3523 2869 227 187 110 BsrGI T'GTAC_A Cuts at: 96 3987 96 Size: 3891 3025 BssHII G'CGCG_C Cuts at: 1925 2500 1925 Size: 575 6341 BssSI C'ACGA_G Cuts at: 3568 4976 5988 3568 Size: 1408 1012 4496 Fragments arranged by size: 4496 1408 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2105 794 Size: 1311 5605 BstBI TT'CG_AA Cuts at: 5720 5720 Size: 6916 BstEII G'GTnAC_C Cuts at: 1184 2819 3943 1184 Size: 1635 1124 4157 Fragments arranged by size: 4157 1635 1124 BstYI r'GATC_y Cuts at: 1972 2850 3165 4246 4613 4998 5015 6467 6553 Size: 878 315 1081 367 385 17 1452 86 Cuts at: 6553 6565 6911 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1081 878 385 367 346 315 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3144 3144 Size: 6916 BtgI C'CryG_G Cuts at: 513 1065 3010 3420 513 Size: 552 1945 410 4009 Fragments arranged by size: 4009 1945 552 410 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6177 Fragments arranged by size: 6177 683 56 BtsI GCAGTG_nn' Cuts at: 3075 3239 3317 4432 5226 5246 3075 Size: 164 78 1115 794 20 4745 Fragments arranged by size: 4745 1115 794 164 78 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6916 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2299 2334 3596 3631 529 Size: 35 1735 35 1262 35 3814 Fragments arranged by size: 3814 1735 1262 35 35 35 DraI TTT'AAA Cuts at: 4220 4220 Size: 6916 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3104 3586 5923 809 Size: 507 1788 482 2337 1802 Fragments arranged by size: 2337 1802 1788 507 482 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3120 3291 3379 Size: 54 1425 50 275 18 1290 171 88 Cuts at: 3379 4363 5252 6881 8 Size: 984 889 1629 43 Fragments arranged by size: 1629 1425 1290 984 889 275 171 88 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4363 1537 Size: 275 2551 4090 Fragments arranged by size: 4090 2551 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2050 2222 2404 3951 4846 1353 Size: 697 172 182 1547 895 3423 Fragments arranged by size: 3423 1547 895 697 182 172 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3434 5503 5877 6023 387 Size: 1064 138 1845 2069 374 146 1280 Fragments arranged by size: 2069 1845 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2936 2936 Size: 6916 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2246 2753 2850 3146 3833 3975 4979 Size: 393 614 507 97 296 687 142 1004 Cuts at: 4979 5576 6363 6631 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1004 787 687 614 597 507 393 296 268 142 97 EcoNI CCTnn'n_nnAGG Cuts at: 2265 2265 Size: 6916 EcoO109I rG'GnC_Cy Cuts at: 2865 2865 Size: 6916 EcoRI G'AATT_C Cuts at: 4225 4603 4225 Size: 378 6538 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3838 4223 3838 Size: 385 6531 FspI TGC'GCA Cuts at: 5422 5422 Size: 6916 HaeII r_GCGC'y Cuts at: 1678 2713 6063 1678 Size: 1035 3350 2531 Fragments arranged by size: 3350 2531 1035 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2931 2963 3171 3203 3460 Size: 32 135 32 1359 32 208 32 257 Cuts at: 3460 3492 3518 3550 3577 3609 5557 5589 5631 Size: 32 26 32 27 32 1948 32 42 Cuts at: 5631 5663 1373 Size: 32 2626 Fragments arranged by size: 2626 1948 1359 257 208 135 42 32 32 32 32 32 32 32 32 32 27 26 HincII GTy'rAC Cuts at: 669 1657 1938 2062 4260 4506 669 Size: 988 281 124 2198 246 3079 Fragments arranged by size: 3079 2198 988 281 246 124 HindIII A'AGCT_T Cuts at: 748 2639 748 Size: 1891 5025 HpaI GTT'AAC Cuts at: 4506 4506 Size: 6916 KpnI G_GTAC'C Cuts at: 4241 4241 Size: 6916 MfeI C'AATT_G Cuts at: 4515 4515 Size: 6916 MluI A'CGCG_T Cuts at: 6792 6792 Size: 6916 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2860 3852 6030 6214 1305 Size: 1555 992 2178 184 2007 Fragments arranged by size: 2178 2007 1555 992 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3293 10 Size: 54 1425 343 1461 3633 Fragments arranged by size: 3633 1461 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2069 2190 3080 3905 4874 5233 5392 Size: 648 903 121 890 825 969 359 159 Cuts at: 5392 518 Size: 2042 Fragments arranged by size: 2042 969 903 890 825 648 359 159 121 MspA1I CmG'CkG Cuts at: 1606 2290 2346 2674 2719 2731 2752 2761 3374 Size: 684 56 328 45 12 21 9 613 Cuts at: 3374 4332 5009 6157 6402 1606 Size: 958 677 1148 245 2120 Fragments arranged by size: 2120 1148 958 684 677 613 328 245 56 45 21 12 9 NaeI GCC'GGC Cuts at: 2039 2039 Size: 6916 NcoI C'CATG_G Cuts at: 513 1065 3010 513 Size: 552 1945 4419 Fragments arranged by size: 4419 1945 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6916 NgoMIV G'CCGG_C Cuts at: 2037 2037 Size: 6916 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6916 NotI GC'GGCC_GC Cuts at: 4363 4363 Size: 6916 NsiI A_TGCA'T Cuts at: 3851 3851 Size: 6916 NspI r_CATG'y Cuts at: 1132 1229 2189 2743 2842 4006 4274 5819 1132 Size: 97 960 554 99 1164 268 1545 2229 Fragments arranged by size: 2229 1545 1164 960 554 268 99 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6916 PciI A'CATG_T Cuts at: 5815 5815 Size: 6916 PflMI CCAn_nnn'nTGG Cuts at: 1108 2674 1108 Size: 1566 5350 PfoI T'CCnGG_A Cuts at: 2266 2853 4047 4118 2266 Size: 587 1194 71 5064 Fragments arranged by size: 5064 1194 587 71 PmeI GTTT'AAAC Cuts at: 4220 4220 Size: 6916 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4985 5017 6498 6530 4985 Size: 32 1481 32 5371 Fragments arranged by size: 5371 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6916 PsiI TTA'TAA Cuts at: 4486 4486 Size: 6916 PspOMI G'GGCC_C Cuts at: 2865 2865 Size: 6916 PspXI vC'TCGA_Gb Cuts at: 2937 2937 Size: 6916 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3992 4024 3992 Size: 32 6884 PstI C_TGCA'G Cuts at: 830 2766 3234 4268 830 Size: 1936 468 1034 3478 Fragments arranged by size: 3478 1936 1034 468 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6916 PvuII CAG'CTG Cuts at: 2290 2674 2719 2731 2752 2761 2290 Size: 384 45 12 21 9 6445 Fragments arranged by size: 6445 384 45 21 12 9 SacI G_AGCT'C Cuts at: 2938 2938 Size: 6916 SalI G'TCGA_C Cuts at: 1936 4258 1936 Size: 2322 4594 SapI GCTCTTCn'nnn_ Cuts at: 3951 3951 Size: 6916 SbfI CC_TGCA'GG Cuts at: 4268 4268 Size: 6916 ScaI AGT'ACT Cuts at: 3952 5164 6876 3952 Size: 1212 1712 3992 Fragments arranged by size: 3992 1712 1212 SfcI C'TryA_G Cuts at: 826 1045 2762 3230 4264 5399 6080 6271 826 Size: 219 1717 468 1034 1135 681 191 1471 Fragments arranged by size: 1717 1471 1135 1034 681 468 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6916 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6916 SmaI CCC'GGG Cuts at: 1872 2507 4243 1872 Size: 635 1736 4545 Fragments arranged by size: 4545 1736 635 SmlI C'TyrA_G Cuts at: 820 1017 1943 2032 2937 5020 5921 6183 820 Size: 197 926 89 905 2083 901 262 1553 Fragments arranged by size: 2083 1553 926 905 901 262 197 89 SnaBI TAC'GTA Cuts at: 493 4707 493 Size: 4214 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6916 SphI G_CATG'C Cuts at: 1132 2189 2842 4274 1132 Size: 1057 653 1432 3774 Fragments arranged by size: 3774 1432 1057 653 SspI AAT'ATT Cuts at: 5 52 4840 5 Size: 47 4788 2081 Fragments arranged by size: 4788 2081 47 StuI AGG'CCT Cuts at: 1517 2443 1517 Size: 926 5990 StyI C'CwwG_G Cuts at: 513 1065 2146 3010 4355 513 Size: 552 1081 864 1345 3074 Fragments arranged by size: 3074 1345 1081 864 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2946 3763 4943 4960 5113 5298 5743 Size: 1255 1366 817 1180 17 153 185 445 Cuts at: 5743 325 Size: 1498 Fragments arranged by size: 1498 1366 1255 1180 817 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2209 2357 2371 Size: 274 80 33 51 1443 232 148 14 Cuts at: 2371 3950 3987 5162 6874 96 Size: 1579 37 1175 1712 138 Fragments arranged by size: 1712 1579 1443 1175 274 232 148 138 80 51 37 33 14 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6876 6908 6876 Size: 32 6884 Tth111I GACn'n_nGTC Cuts at: 1316 2876 1316 Size: 1560 5356 XbaI T'CTAG_A Cuts at: 4252 4252 Size: 6916 XhoI C'TCGA_G Cuts at: 1943 2937 1943 Size: 994 5922 XmaI C'CCGG_G Cuts at: 1870 2505 4241 1870 Size: 635 1736 4545 Fragments arranged by size: 4545 1736 635 XmnI GAAnn'nnTTC Cuts at: 5045 5045 Size: 6916 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3247 4724 276 Size: 53 83 186 936 1713 1477 2468 Fragments arranged by size: 2468 1713 1477 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FseI FspAI KasI NarI NruI PacI PmlI PpuMI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI