Restriction map for
pFN21AB3307
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AgeI AhdI Aor51HI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII BamHI BbeI BbrPI
BciVI BclI BfrI BglII BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsiEI BsiWI BsmBI BsmI BspDI
BspHI BspMI BsrDI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoT22I FbaI FspI HaeII Hin1I
HpaI KasI KpnI KspI MamI
MfeI MluI MunI MvnI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SbfI ScaI SexAI SfiI SfoI
SfuI SmaI SnaBI SphI Sse8387I
SspI SwaI Tth111I XbaI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 75 GTCGAC GT^[AC][GT]AC
AccIII 584 TCCGGA T^CCGGA
AflIII 1121 ACATGT A^C[GA][CT]GT
Alw44I 1624 GTGCAC G^TGCAC
AocI 2089 CCTGAGG CC^T[ACGT]AGG
ApaLI 1624 GTGCAC G^TGCAC
AvaI 2054 CTCGAG C^[CT]CG[GA]G
AvrII 438 CCTAGG C^CTAGG
BlnI 438 CCTAGG C^CTAGG
BsaWI 584 TCCGGA [AT]^CCGG[AT]
BseAI 584 TCCGGA T^CCGGA
BsoBI 2054 CTCGAG C^[CT]CG[GA]G
Bsp1407I 1387 TGTACA T^GTACA
BspEI 584 TCCGGA T^CCGGA
BspLU11I 1121 ACATGT A^CATGT
BsrGI 1387 TGTACA T^GTACA
Bsu36I 2089 CCTGAGG CC^T[ACGT]AGG
CfoI 909 GCGC GCG^C
EarI 931 CTCTTC CTCTTC(1/4)
Eco81I 2089 CCTGAGG CC^T[ACGT]AGG
EcoRV 186 GATATC GAT^ATC
FseI 1645 GGCCGGCC GGCCGG^CC
HhaI 909 GCGC GCG^C
HinP1I 909 GCGC G^CGC
HindIII 643 AAGCTT A^AGCTT
Ksp632I 931 CTCTTC CTCTTC(1/4)
MaeII 1622 ACGT A^CGT
MroI 584 TCCGGA T^CCGGA
NaeI 1646 GCCGGC GCC^GGC
NgoMIV 1646 GCCGGC G^CCGGC
NspI 1121 ACATGT [GA]CATG^[CT]
PaeR7I 2054 CTCGAG C^TCGAG
SacI 928 GAGCTC GAGCT^C
SalI 75 GTCGAC G^TCGAC
SapI 930 GCTCTTC GCTCTTC(1/4)
SgrAI 904 CACCGGCG C[GA]^CCGG[CT]G
SpeI 1002 ACTAGT A^CTAGT
SspBI 1387 TGTACA T^GTACA
StuI 1153 AGGCCT AGG^CCT
XcmI 903 CCACCGGCGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2054 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 1561 GAATTT [GA]^AATT[CT]
2147 AAATTT
AfaI 467 GTAC GT^AC
1388 GTAC
ApoI 1561 GAATTT [GA]^AATT[CT]
2147 AAATTT
BalI 1819 TGGCCA TGG^CCA
2013 TGGCCA
BanI 1597 GGTGCC G^G[CT][GA]CC
1726 GGTGCC
BbsI 1117 GAAGAC GAAGAC(2/6)
795 GTCTTC
BbvCI 1956 CCTCAGC CCTCAGC(-5/-2)
858 GCTGAGG
BcgI 1348 CGACTGCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
67 GCAGTTGTGTCG
BglI 1821 GCCACTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1885 GCCAAAGAGGC
BpuAI 1117 GAAGAC GAAGAC(2/6)
795 GTCTTC
BseRI 118 GAGGAG GAGGAG(10/8)
658 GAGGAG
BssSI 977 CACGAG CACGAG(-5/-1)
2124 CACGAG
BstYI 1261 AGATCC [GA]^GATC[CT]
1412 AGATCC
HgaI 1372 GACGC GACGC(5/10)
1737 GCGTC
HincII 75 GTCGAC GT[CT]^[GA]AC
709 GTTGAC
HindII 75 GTCGAC GT[CT]^[GA]AC
709 GTTGAC
HphI 1050 GGTGA GGTGA(8/7)
1752 TCACC
MflI 1261 AGATCC [GA]^GATC[CT]
1412 AGATCC
MluNI 1819 TGGCCA TGG^CCA
2013 TGGCCA
MscI 1819 TGGCCA TGG^CCA
2013 TGGCCA
PleI 1942 GACTC GAGTC(4/5)
2052 GACTC
RsaI 467 GTAC GT^AC
1388 GTAC
XhoII 1261 AGATCC [GA]^GATC[CT]
1412 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AluI AlwI AlwNI ApaI
AspHI AvaII BanII BbvI BcnI
BfaI BmyI BpmI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI Cac8I Cfr10I
Cfr13I DdeI DpnII DraII EaeI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII ItaI MaeI MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI PpuMI PstI
PvuII Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I