(Circular) (Six-base) MAPSORT of: pFN21AB3307.seq Check: 2094 from: 1 to: 6859 pFN21AB3307 6859 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4669 278 Size: 53 83 186 936 3133 2468 Fragments arranged by size: 3133 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2081 4202 1937 Size: 144 2121 4594 Fragments arranged by size: 4594 2121 144 Acc65I G'GTAC_C Cuts at: 4180 4180 Size: 6859 AclI AA'CG_TT Cuts at: 1563 4986 5359 1563 Size: 3423 373 3063 Fragments arranged by size: 3423 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2786 2787 3581 4922 6306 6574 1632 Size: 1154 1 794 1341 1384 268 1917 Fragments arranged by size: 1917 1384 1341 1154 794 268 1 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6662 AflIII A'CryG_T Cuts at: 1203 3126 5758 6735 1203 Size: 1923 2632 977 1327 Fragments arranged by size: 2632 1923 1327 977 AgeI A'CCGG_T Cuts at: 5690 5690 Size: 6859 AhdI GACnn_n'nnGTC Cuts at: 5588 5588 Size: 6859 AleI CACnn'nnGTG Cuts at: 1166 3661 1166 Size: 2495 4364 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3747 3826 1184 Size: 2563 79 4217 Fragments arranged by size: 4217 2563 79 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2771 2803 2771 Size: 32 6827 AlwNI CAG_nnn'CTG Cuts at: 2285 2294 2348 2432 2576 3152 3270 3559 3910 Size: 9 54 84 144 576 118 289 351 Cuts at: 3910 3950 4111 4602 6174 2285 Size: 40 161 491 1572 2970 Fragments arranged by size: 2970 1572 576 491 351 289 161 144 118 84 54 40 9 ApaI G_GGCC'C Cuts at: 2732 2812 3985 2732 Size: 80 1173 5606 Fragments arranged by size: 5606 1173 80 ApaLI G'TGCA_C Cuts at: 3629 4916 6072 3629 Size: 1287 1156 4416 Fragments arranged by size: 4416 1287 1156 ApoI r'AATT_y Cuts at: 1493 3566 4152 4168 4394 4546 6655 1493 Size: 2073 586 16 226 152 2109 1697 Fragments arranged by size: 2109 2073 1697 586 226 152 16 AseI AT'TA_AT Cuts at: 160 1032 5413 160 Size: 872 4381 1606 Fragments arranged by size: 4381 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6859 AvaI C'yCGr_G Cuts at: 1870 1943 4059 4184 1870 Size: 73 2116 125 4545 Fragments arranged by size: 4545 2116 125 73 AvrII C'CTAG_G Cuts at: 2443 2443 Size: 6859 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2274 2307 2274 Size: 33 6826 BamHI G'GATC_C Cuts at: 4189 4556 4189 Size: 367 6492 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3602 3731 4180 5635 618 Size: 325 217 627 1815 129 449 1455 1842 Fragments arranged by size: 1842 1815 1455 627 449 325 217 129 BanII G_rGCy'C Cuts at: 1391 2531 2732 2812 2937 2960 3310 3985 4178 Size: 1140 201 80 125 23 350 675 193 Cuts at: 4178 1391 Size: 4072 Fragments arranged by size: 4072 1140 675 350 201 193 125 80 23 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2793 3129 928 Size: 971 894 336 4658 Fragments arranged by size: 4658 971 894 336 BbvCI CC'TCA_GC Cuts at: 2864 3962 2864 Size: 1098 5761 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2061 2095 3342 3376 5050 5084 1903 Size: 34 124 34 1247 34 1674 34 3678 Fragments arranged by size: 3678 1674 1247 124 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4751 5961 4751 Size: 1210 5649 BclI T'GATC_A Cuts at: 1548 1548 Size: 6859 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3832 3896 5470 Size: 107 122 71 631 605 2160 64 1574 Cuts at: 5470 136 Size: 1525 Fragments arranged by size: 2160 1574 1525 631 605 122 107 71 64 BglII A'GATC_T Cuts at: 6854 6854 Size: 6859 BlpI GC'TnA_GC Cuts at: 4276 4276 Size: 6859 Bme1580I G_kGCm'C Cuts at: 1790 2732 2812 3105 3633 3736 3985 4920 6076 Size: 942 80 293 528 103 249 935 1156 Cuts at: 6076 1790 Size: 2573 Fragments arranged by size: 2573 1156 942 935 528 293 249 103 80 BmgBI CAC'GTC Cuts at: 1318 3628 1318 Size: 2310 4549 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3115 4590 5548 Size: 416 53 83 399 24 1698 1475 958 Cuts at: 5548 442 Size: 1753 Fragments arranged by size: 1753 1698 1475 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6859 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3785 3785 Size: 6859 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2223 2345 2389 3615 3695 3758 5519 1239 Size: 984 122 44 1226 80 63 1761 2579 Fragments arranged by size: 2579 1761 1226 984 122 80 63 44 Bpu10I CC'TnA_GC Cuts at: 2139 2864 3962 2139 Size: 725 1098 5036 Fragments arranged by size: 5036 1098 725 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2283 3200 4984 5849 6147 2283 Size: 917 1784 865 298 2995 Fragments arranged by size: 2995 1784 917 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5522 882 Size: 617 4023 2219 Fragments arranged by size: 4023 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4650 493 Size: 711 3446 2702 Fragments arranged by size: 3446 2702 711 BsaBI GATnn'nnATC Cuts at: 6853 6853 Size: 6859 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4666 5048 5683 275 Size: 53 83 186 936 3133 382 635 1451 Fragments arranged by size: 3133 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2589 5292 5690 5964 6111 6522 2589 Size: 2703 398 274 147 411 2926 Fragments arranged by size: 2926 2703 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6634 Fragments arranged by size: 6634 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2138 2678 1186 Size: 952 540 5367 Fragments arranged by size: 5367 952 540 BseYI C'CCAG_C Cuts at: 3164 3984 6062 6558 3164 Size: 820 2078 496 3465 Fragments arranged by size: 3465 2078 820 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3158 3219 4124 1163 Size: 573 1422 61 905 3898 Fragments arranged by size: 3898 1422 905 573 61 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4309 5070 6098 1540 Size: 275 186 2308 761 1028 2301 Fragments arranged by size: 2308 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2937 3633 3771 4920 5005 6076 2937 Size: 696 138 1149 85 1071 3720 Fragments arranged by size: 3720 1149 1071 696 138 85 BsmI GAATG_Cn' Cuts at: 4370 4463 4370 Size: 93 6766 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2531 2732 2812 2937 2960 3105 3310 Size: 399 741 201 80 125 23 145 205 Cuts at: 3310 3633 3736 3771 3985 4178 4920 5005 6076 Size: 323 103 35 214 193 742 85 1071 Cuts at: 6076 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 741 399 323 214 205 201 193 145 125 103 85 80 35 23 BspEI T'CCGG_A Cuts at: 2589 6522 2589 Size: 3933 2926 BspHI T'CATG_A Cuts at: 4748 6478 4748 Size: 1730 5129 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4214 844 Size: 3370 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2107 2329 2551 4746 1394 Size: 429 284 222 222 2195 3507 Fragments arranged by size: 3507 2195 429 284 222 222 BsrDI GCAATG_nn' Cuts at: 65 1248 5354 5528 65 Size: 1183 4106 174 1396 Fragments arranged by size: 4106 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2910 3258 3651 5503 5690 1700 Size: 110 1100 348 393 1852 187 2869 Fragments arranged by size: 2869 1852 1100 393 348 187 110 BsrGI T'GTAC_A Cuts at: 96 3392 96 Size: 3296 3563 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6859 BssSI C'ACGA_G Cuts at: 2982 4129 4919 5931 2982 Size: 1147 790 1012 3910 Fragments arranged by size: 3910 1147 1012 790 BstAPI GCAn_nnn'nTGC Cuts at: 794 2285 2294 3910 794 Size: 1491 9 1616 3743 Fragments arranged by size: 3743 1616 1491 9 BstBI TT'CG_AA Cuts at: 5663 5663 Size: 6859 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6859 BstYI r'GATC_y Cuts at: 1972 3266 3417 4189 4556 4941 4958 6410 6496 Size: 1294 151 772 367 385 17 1452 86 Cuts at: 6496 6508 6854 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1294 772 385 367 346 151 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4095 4095 Size: 6859 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5368 Fragments arranged by size: 5368 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6120 Fragments arranged by size: 6120 683 56 BtsI GCAGTG_nn' Cuts at: 2295 2397 2819 3238 3771 4375 5169 5189 2295 Size: 102 422 419 533 604 794 20 3965 Fragments arranged by size: 3965 794 604 533 422 419 102 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6859 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2079 2114 529 Size: 35 1515 35 5274 Fragments arranged by size: 5274 1515 35 35 DraI TTT'AAA Cuts at: 4163 4163 Size: 6859 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5866 809 Size: 507 4550 1802 Fragments arranged by size: 4550 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3494 3653 3824 Size: 54 1425 50 275 18 1664 159 171 Cuts at: 3824 4018 4306 5195 6824 8 Size: 194 288 889 1629 43 Fragments arranged by size: 1664 1629 1425 889 288 275 194 171 159 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4306 1537 Size: 275 2494 4090 Fragments arranged by size: 4090 2494 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2942 4789 1353 Size: 1589 1847 3423 Fragments arranged by size: 3423 1847 1589 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3464 3965 5446 5820 5966 387 Size: 1064 138 1875 501 1481 374 146 1280 Fragments arranged by size: 1875 1481 1280 1064 501 374 146 138 EcoICRI GAG'CTC Cuts at: 2935 2935 Size: 6859 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2223 2345 2389 2786 2787 3581 3615 Size: 393 591 122 44 397 1 794 34 Cuts at: 3615 3695 3758 4922 5519 6306 6574 1239 Size: 80 63 1164 597 787 268 1524 Fragments arranged by size: 1524 1164 794 787 597 591 397 393 268 122 80 63 44 34 1 EcoNI CCTnn'n_nnAGG Cuts at: 2120 2507 2590 2723 2794 3551 2120 Size: 387 83 133 71 757 5428 Fragments arranged by size: 5428 757 387 133 83 71 EcoO109I rG'GnC_Cy Cuts at: 2728 2729 2808 2809 3514 3544 3556 3583 4135 Size: 1 79 1 705 30 12 27 552 Cuts at: 4135 2728 Size: 5452 Fragments arranged by size: 5452 705 552 79 30 27 12 1 1 EcoRI G'AATT_C Cuts at: 4168 4546 4168 Size: 378 6481 EcoRV GAT'ATC Cuts at: 2193 2193 Size: 6859 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2671 2671 Size: 6859 FseI GG_CCGG'CC Cuts at: 3655 3655 Size: 6859 FspI TGC'GCA Cuts at: 5365 5365 Size: 6859 HaeII r_GCGC'y Cuts at: 1678 6006 1678 Size: 4328 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3575 3607 3693 3725 3932 Size: 32 135 32 2003 32 86 32 207 Cuts at: 3932 3945 3964 3977 5500 5532 5574 5606 1373 Size: 13 19 13 1523 32 42 32 2626 Fragments arranged by size: 2626 2003 1523 207 135 86 42 32 32 32 32 32 32 19 13 13 HincII GTy'rAC Cuts at: 669 1657 1938 2082 2716 4203 4449 669 Size: 988 281 144 634 1487 246 3079 Fragments arranged by size: 3079 1487 988 634 281 246 144 HindIII A'AGCT_T Cuts at: 748 2648 748 Size: 1900 4959 HpaI GTT'AAC Cuts at: 4449 4449 Size: 6859 KpnI G_GTAC'C Cuts at: 4184 4184 Size: 6859 MfeI C'AATT_G Cuts at: 4458 4458 Size: 6859 MluI A'CGCG_T Cuts at: 6735 6735 Size: 6859 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2705 3712 5973 6157 1305 Size: 1400 1007 2261 184 2007 Fragments arranged by size: 2261 2007 1400 1007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3826 4020 10 Size: 54 1425 343 1994 194 2849 Fragments arranged by size: 2849 1994 1425 343 194 54 MslI CAynn'nnrTG Cuts at: 518 1166 2791 3661 3663 3863 4817 5176 5335 Size: 648 1625 870 2 200 954 359 159 Cuts at: 5335 518 Size: 2042 Fragments arranged by size: 2042 1625 954 870 648 359 200 159 2 MspA1I CmG'CkG Cuts at: 1606 2432 2573 3406 4275 4952 6100 6345 1606 Size: 826 141 833 869 677 1148 245 2120 Fragments arranged by size: 2120 1148 869 833 826 677 245 141 NaeI GCC'GGC Cuts at: 3653 3653 Size: 6859 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5368 Fragments arranged by size: 5368 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6859 NgoMIV G'CCGG_C Cuts at: 3651 3651 Size: 6859 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6859 NotI GC'GGCC_GC Cuts at: 4306 4306 Size: 6859 NspI r_CATG'y Cuts at: 1132 1229 3130 4217 5762 1132 Size: 97 1901 1087 1545 2229 Fragments arranged by size: 2229 1901 1545 1087 97 PasI CC'CwG_GG Cuts at: 1348 2960 3579 3646 1348 Size: 1612 619 67 4561 Fragments arranged by size: 4561 1612 619 67 PciI A'CATG_T Cuts at: 3126 5758 3126 Size: 2632 4227 PflMI CCAn_nnn'nTGG Cuts at: 1108 2419 3270 3853 1108 Size: 1311 851 583 4114 Fragments arranged by size: 4114 1311 851 583 PfoI T'CCnGG_A Cuts at: 2200 2200 Size: 6859 PmeI GTTT'AAAC Cuts at: 4163 4163 Size: 6859 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2919 2951 4928 4960 6441 6473 2919 Size: 32 1977 32 1481 32 3305 Fragments arranged by size: 3305 1977 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3514 3544 3583 4135 3514 Size: 30 39 552 6238 Fragments arranged by size: 6238 552 39 30 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6859 PsiI TTA'TAA Cuts at: 4429 4429 Size: 6859 PspOMI G'GGCC_C Cuts at: 2728 2808 3981 2728 Size: 80 1173 5606 Fragments arranged by size: 5606 1173 80 PstI C_TGCA'G Cuts at: 830 2290 2462 2826 3922 4211 830 Size: 1460 172 364 1096 289 3478 Fragments arranged by size: 3478 1460 1096 364 289 172 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6859 PvuII CAG'CTG Cuts at: 2432 2573 3406 2432 Size: 141 833 5885 Fragments arranged by size: 5885 833 141 SacI G_AGCT'C Cuts at: 2937 2937 Size: 6859 SalI G'TCGA_C Cuts at: 1936 2080 4201 1936 Size: 144 2121 4594 Fragments arranged by size: 4594 2121 144 SanDI GG'GwC_CC Cuts at: 3514 4135 3514 Size: 621 6238 SapI GCTCTTCn'nnn_ Cuts at: 2942 2942 Size: 6859 SbfI CC_TGCA'GG Cuts at: 4211 4211 Size: 6859 ScaI AGT'ACT Cuts at: 5107 6819 5107 Size: 1712 5147 SfcI C'TryA_G Cuts at: 826 1045 2286 2458 2822 3918 4121 4207 5342 Size: 219 1241 172 364 1096 203 86 1135 Cuts at: 5342 6023 6214 826 Size: 681 191 1471 Fragments arranged by size: 1471 1241 1135 1096 681 364 219 203 191 172 86 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6859 SgrAI Cr'CCGG_yG Cuts at: 1700 2910 1700 Size: 1210 5649 SmaI CCC'GGG Cuts at: 1872 4186 1872 Size: 2314 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2298 3215 4059 4963 5864 6126 Size: 197 926 355 917 844 904 901 262 Cuts at: 6126 820 Size: 1553 Fragments arranged by size: 1553 926 917 904 901 844 355 262 197 SnaBI TAC'GTA Cuts at: 493 4650 493 Size: 4157 2702 SpeI A'CTAG_T Cuts at: 152 3007 152 Size: 2855 4004 SphI G_CATG'C Cuts at: 1132 4217 1132 Size: 3085 3774 SspI AAT'ATT Cuts at: 5 52 4783 5 Size: 47 4731 2081 Fragments arranged by size: 4731 2081 47 StuI AGG'CCT Cuts at: 1517 3160 1517 Size: 1643 5216 StyI C'CwwG_G Cuts at: 513 1065 2004 2041 2334 2443 4075 4298 513 Size: 552 939 37 293 109 1632 223 3074 Fragments arranged by size: 3074 1632 939 552 293 223 109 37 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2426 2688 4886 4903 5056 5241 5686 Size: 1255 846 262 2198 17 153 185 445 Cuts at: 5686 325 Size: 1498 Fragments arranged by size: 2198 1498 1255 846 445 262 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2471 3392 5105 Size: 274 80 33 51 1443 494 921 1713 Cuts at: 5105 6817 96 Size: 1712 138 Fragments arranged by size: 1713 1712 1443 921 494 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6819 6851 6819 Size: 32 6827 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6859 XbaI T'CTAG_A Cuts at: 4195 4195 Size: 6859 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2915 2915 Size: 6859 XhoI C'TCGA_G Cuts at: 1943 4059 1943 Size: 2116 4743 XmaI C'CCGG_G Cuts at: 1870 4184 1870 Size: 2314 4545 XmnI GAAnn'nnTTC Cuts at: 4988 4988 Size: 6859 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4667 276 Size: 53 83 186 936 3133 2468 Fragments arranged by size: 3133 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI