Restriction map for
pFN21AB3307
(Linear) (Six-base) MAPSORT of: KIBB3307.seq Check: 9957 from: 1 to: 2154
KIBB3307.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AccI GT'mk_AC
Cuts at: 0 76 2154
Size: 76 2078
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 781 782 1576 2154
Size: 781 1 794 578
Fragments arranged by size:
794 781 578 1
AflIII A'CryG_T
Cuts at: 0 1121 2154
Size: 1121 1033
AleI CACnn'nnGTG
Cuts at: 0 1656 2154
Size: 1656 498
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1742 1821 2154
Size: 1742 79 333
Fragments arranged by size:
1742 333 79
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 766 798 2154
Size: 766 32 1356
Fragments arranged by size:
1356 766 32
AlwNI CAG_nnn'CTG
Cuts at: 0 280 289 343 427 571 1147 1265 1554
Size: 280 9 54 84 144 576 118 289
Cuts at: 1554 1905 1945 2106 2154
Size: 351 40 161 48
Fragments arranged by size:
576 351 289 280 161 144 118 84
54 48 40 9
ApaI G_GGCC'C
Cuts at: 0 727 807 1980 2154
Size: 727 80 1173 174
Fragments arranged by size:
1173 727 174 80
ApaLI G'TGCA_C
Cuts at: 0 1624 2154
Size: 1624 530
ApoI r'AATT_y
Cuts at: 0 1561 2147 2154
Size: 1561 586 7
Fragments arranged by size:
1561 586 7
AvaI C'yCGr_G
Cuts at: 0 2054 2154
Size: 2054 100
AvrII C'CTAG_G
Cuts at: 0 438 2154
Size: 438 1716
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 269 302 2154
Size: 269 33 1852
Fragments arranged by size:
1852 269 33
BanI G'GyrC_C
Cuts at: 0 1597 1726 2154
Size: 1597 129 428
Fragments arranged by size:
1597 428 129
BanII G_rGCy'C
Cuts at: 0 526 727 807 932 955 1305 1980 2154
Size: 526 201 80 125 23 350 675 174
Fragments arranged by size:
675 526 350 201 174 125 80 23
BbsI GAAGACnn'nnnn_
Cuts at: 0 788 1124 2154
Size: 788 336 1030
Fragments arranged by size:
1030 788 336
BbvCI CC'TCA_GC
Cuts at: 0 859 1957 2154
Size: 859 1098 197
Fragments arranged by size:
1098 859 197
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 56 90 1337 1371 2154
Size: 56 34 1247 34 783
Fragments arranged by size:
1247 783 56 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1827 1891 2154
Size: 1827 64 263
Fragments arranged by size:
1827 263 64
Bme1580I G_kGCm'C
Cuts at: 0 727 807 1100 1628 1731 1980 2154
Size: 727 80 293 528 103 249 174
Fragments arranged by size:
727 528 293 249 174 103 80
BmgBI CAC'GTC
Cuts at: 0 1623 2154
Size: 1623 531
BmrI ACTGGGnnnn_n'
Cuts at: 0 1110 2154
Size: 1110 1044
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1780 2154
Size: 1780 374
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 340 384 1610 1690 1753 2154
Size: 218 122 44 1226 80 63 401
Fragments arranged by size:
1226 401 218 122 80 63 44
Bpu10I CC'TnA_GC
Cuts at: 0 134 859 1957 2154
Size: 134 725 1098 197
Fragments arranged by size:
1098 725 197 134
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 278 1195 2154
Size: 278 917 959
Fragments arranged by size:
959 917 278
BsaWI w'CCGG_w
Cuts at: 0 584 2154
Size: 584 1570
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 133 673 2154
Size: 133 540 1481
Fragments arranged by size:
1481 540 133
BseYI C'CCAG_C
Cuts at: 0 1159 1979 2154
Size: 1159 820 175
Fragments arranged by size:
1159 820 175
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1153 1214 2119 2154
Size: 1153 61 905 35
Fragments arranged by size:
1153 905 61 35
BsiHKAI G_wGCw'C
Cuts at: 0 932 1628 1766 2154
Size: 932 696 138 388
Fragments arranged by size:
932 696 388 138
Bsp1286I G_dGCh'C
Cuts at: 0 526 727 807 932 955 1100 1305 1628
Size: 526 201 80 125 23 145 205 323
Cuts at: 1628 1731 1766 1980 2154
Size: 103 35 214 174
Fragments arranged by size:
526 323 214 205 201 174 145 125
103 80 35 23
BspEI T'CCGG_A
Cuts at: 0 584 2154
Size: 584 1570
BsrBI CCG'CTC
Cuts at: 0 102 324 546 2154
Size: 102 222 222 1608
Fragments arranged by size:
1608 222 222 102
BsrFI r'CCGG_y
Cuts at: 0 905 1253 1646 2154
Size: 905 348 393 508
Fragments arranged by size:
905 508 393 348
BsrGI T'GTAC_A
Cuts at: 0 1387 2154
Size: 1387 767
BssSI C'ACGA_G
Cuts at: 0 977 2124 2154
Size: 977 1147 30
Fragments arranged by size:
1147 977 30
BstAPI GCAn_nnn'nTGC
Cuts at: 0 280 289 1905 2154
Size: 280 9 1616 249
Fragments arranged by size:
1616 280 249 9
BstYI r'GATC_y
Cuts at: 0 1261 1412 2154
Size: 1261 151 742
Fragments arranged by size:
1261 742 151
Bsu36I CC'TnA_GG
Cuts at: 0 2090 2154
Size: 2090 64
BtsI GCAGTG_nn'
Cuts at: 0 290 392 814 1233 1766 2154
Size: 290 102 422 419 533 388
Fragments arranged by size:
533 422 419 388 290 102
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 74 109 2154
Size: 74 35 2045
Fragments arranged by size:
2045 74 35
EaeI y'GGCC_r
Cuts at: 0 1489 1648 1819 2013 2154
Size: 1489 159 171 194 141
Fragments arranged by size:
1489 194 171 159 141
EarI CTCTTCn'nnn_
Cuts at: 0 937 2154
Size: 937 1217
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1459 1960 2154
Size: 1459 501 194
Fragments arranged by size:
1459 501 194
EcoICRI GAG'CTC
Cuts at: 0 930 2154
Size: 930 1224
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 340 384 781 782 1576 1610 1690
Size: 218 122 44 397 1 794 34 80
Cuts at: 1690 1753 2154
Size: 63 401
Fragments arranged by size:
794 401 397 218 122 80 63 44
34 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 115 502 585 718 789 1546 2154
Size: 115 387 83 133 71 757 608
Fragments arranged by size:
757 608 387 133 115 83 71
EcoO109I rG'GnC_Cy
Cuts at: 0 723 724 803 804 1509 1539 1551 1578
Size: 723 1 79 1 705 30 12 27
Cuts at: 1578 2130 2154
Size: 552 24
Fragments arranged by size:
723 705 552 79 30 27 24 12
1 1
EcoRV GAT'ATC
Cuts at: 0 188 2154
Size: 188 1966
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 666 2154
Size: 666 1488
FseI GG_CCGG'CC
Cuts at: 0 1650 2154
Size: 1650 504
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1570 1602 1688 1720 1927 1940 1959 1972
Size: 1570 32 86 32 207 13 19 13
Cuts at: 1972 2154
Size: 182
Fragments arranged by size:
1570 207 182 86 32 32 19 13
13
HincII GTy'rAC
Cuts at: 0 77 711 2154
Size: 77 634 1443
Fragments arranged by size:
1443 634 77
HindIII A'AGCT_T
Cuts at: 0 643 2154
Size: 643 1511
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 700 1707 2154
Size: 700 1007 447
Fragments arranged by size:
1007 700 447
MscI TGG'CCA
Cuts at: 0 1821 2015 2154
Size: 1821 194 139
Fragments arranged by size:
1821 194 139
MslI CAynn'nnrTG
Cuts at: 0 786 1656 1658 1858 2154
Size: 786 870 2 200 296
Fragments arranged by size:
870 786 296 200 2
MspA1I CmG'CkG
Cuts at: 0 427 568 1401 2154
Size: 427 141 833 753
Fragments arranged by size:
833 753 427 141
NaeI GCC'GGC
Cuts at: 0 1648 2154
Size: 1648 506
NgoMIV G'CCGG_C
Cuts at: 0 1646 2154
Size: 1646 508
NspI r_CATG'y
Cuts at: 0 1125 2154
Size: 1125 1029
PasI CC'CwG_GG
Cuts at: 0 955 1574 1641 2154
Size: 955 619 67 513
Fragments arranged by size:
955 619 513 67
PciI A'CATG_T
Cuts at: 0 1121 2154
Size: 1121 1033
PflMI CCAn_nnn'nTGG
Cuts at: 0 414 1265 1848 2154
Size: 414 851 583 306
Fragments arranged by size:
851 583 414 306
PfoI T'CCnGG_A
Cuts at: 0 195 2154
Size: 195 1959
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 914 946 2154
Size: 914 32 1208
Fragments arranged by size:
1208 914 32
PpuMI rG'GwC_Cy
Cuts at: 0 1509 1539 1578 2130 2154
Size: 1509 30 39 552 24
Fragments arranged by size:
1509 552 39 30 24
PspOMI G'GGCC_C
Cuts at: 0 723 803 1976 2154
Size: 723 80 1173 178
Fragments arranged by size:
1173 723 178 80
PstI C_TGCA'G
Cuts at: 0 285 457 821 1917 2154
Size: 285 172 364 1096 237
Fragments arranged by size:
1096 364 285 237 172
PvuII CAG'CTG
Cuts at: 0 427 568 1401 2154
Size: 427 141 833 753
Fragments arranged by size:
833 753 427 141
SacI G_AGCT'C
Cuts at: 0 932 2154
Size: 932 1222
SalI G'TCGA_C
Cuts at: 0 75 2154
Size: 75 2079
SanDI GG'GwC_CC
Cuts at: 0 1509 2130 2154
Size: 1509 621 24
Fragments arranged by size:
1509 621 24
SapI GCTCTTCn'nnn_
Cuts at: 0 937 2154
Size: 937 1217
SfcI C'TryA_G
Cuts at: 0 281 453 817 1913 2116 2154
Size: 281 172 364 1096 203 38
Fragments arranged by size:
1096 364 281 203 172 38
SgrAI Cr'CCGG_yG
Cuts at: 0 905 2154
Size: 905 1249
SmlI C'TyrA_G
Cuts at: 0 293 1210 2054 2154
Size: 293 917 844 100
Fragments arranged by size:
917 844 293 100
SpeI A'CTAG_T
Cuts at: 0 1002 2154
Size: 1002 1152
StuI AGG'CCT
Cuts at: 0 1155 2154
Size: 1155 999
StyI C'CwwG_G
Cuts at: 0 36 329 438 2070 2154
Size: 36 293 109 1632 84
Fragments arranged by size:
1632 293 109 84 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 421 683 2154
Size: 421 262 1471
Fragments arranged by size:
1471 421 262
TatI w'GTAC_w
Cuts at: 0 466 1387 2154
Size: 466 921 767
Fragments arranged by size:
921 767 466
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 910 2154
Size: 910 1244
XhoI C'TCGA_G
Cuts at: 0 2054 2154
Size: 2054 100
Enzymes that do cut:
AccI AcuI AflIII AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AvaI AvrII BaeI BanI BanII BbsI
BbvCI BcgI BglI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspEI BsrBI BsrFI BsrGI BssSI BstAPI BstYI Bsu36I
BtsI CspCI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRV FalI FseI Hin4I HincII HindIII MmeI
MscI MslI MspA1I NaeI NgoMIV NspI PasI PciI
PflMI PfoI PpiI PpuMI PspOMI PstI PvuII SacI
SalI SanDI SapI SfcI SgrAI SmlI SpeI StuI
StyI TaqII TatI XcmI XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AscI AseI AsiSI BamHI BbeI BciVI BclI BfrBI
BglII BlpI BmtI BsaI BsaAI BsaBI BsaHI BsaXI
BsiEI BsiWI BsmI BsmBI BspHI BspMI BsrDI BssHII
BstBI BstEII BstXI BstZ17I BtgI BtgZI ClaI DraI
DraIII DrdI EagI EcoRI FspI FspAI HaeII HpaI
KasI KpnI MfeI MluI NarI NcoI NdeI NheI
NotI NruI NsiI PacI PmeI PmlI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SbfI ScaI SexAI
SfiI SfoI SgfI SmaI SnaBI SphI SrfI SspI
SwaI TstI Tth111I XbaI XmaI XmnI ZraI