(Circular) (Six-base) MAPSORT of: pFN21AB3300.seq Check: 2608 from: 1 to: 6874 pFN21AB3300 6874 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4127 4127 Size: 6874 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4684 278 Size: 53 83 186 936 3148 2468 Fragments arranged by size: 3148 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2562 4217 1937 Size: 625 1655 4594 Fragments arranged by size: 4594 1655 625 Acc65I G'GTAC_C Cuts at: 4195 4195 Size: 6874 AclI AA'CG_TT Cuts at: 1563 5001 5374 1563 Size: 3438 373 3063 Fragments arranged by size: 3438 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2441 2843 2876 3008 4937 6321 6589 1632 Size: 809 402 33 132 1929 1384 268 1917 Fragments arranged by size: 1929 1917 1384 809 402 268 132 33 AfeI AGC'GCT Cuts at: 3527 3527 Size: 6874 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6677 AflIII A'CryG_T Cuts at: 1203 5773 6750 1203 Size: 4570 977 1327 Fragments arranged by size: 4570 1327 977 AgeI A'CCGG_T Cuts at: 5705 5705 Size: 6874 AhdI GACnn_n'nnGTC Cuts at: 3496 5603 3496 Size: 2107 4767 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6874 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2183 2331 3384 1184 Size: 999 148 1053 4674 Fragments arranged by size: 4674 1053 999 148 AlwNI CAG_nnn'CTG Cuts at: 2509 2932 3028 3181 3298 3343 3556 3851 3889 Size: 423 96 153 117 45 213 295 38 Cuts at: 3889 4111 4617 6189 2509 Size: 222 506 1572 3194 Fragments arranged by size: 3194 1572 506 423 295 222 213 153 117 96 45 38 ApaI G_GGCC'C Cuts at: 2718 3535 2718 Size: 817 6057 ApaLI G'TGCA_C Cuts at: 4931 6087 4931 Size: 1156 5718 ApoI r'AATT_y Cuts at: 1493 4183 4409 4561 6670 1493 Size: 2690 226 152 2109 1697 Fragments arranged by size: 2690 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5428 160 Size: 872 4396 1606 Fragments arranged by size: 4396 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6874 AvaI C'yCGr_G Cuts at: 1870 1943 2697 2807 4199 1870 Size: 73 754 110 1392 4545 Fragments arranged by size: 4545 1392 754 110 73 BamHI G'GATC_C Cuts at: 3018 3906 4204 4571 3018 Size: 888 298 367 5321 Fragments arranged by size: 5321 888 367 298 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2585 2820 4195 5650 618 Size: 325 217 627 798 235 1375 1455 1842 Fragments arranged by size: 1842 1455 1375 798 627 325 235 217 BanII G_rGCy'C Cuts at: 1391 2127 2718 3535 3591 3818 4193 1391 Size: 736 591 817 56 227 375 4072 Fragments arranged by size: 4072 817 736 591 375 227 56 BbeI G_GCGC'C Cuts at: 2824 2824 Size: 6874 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5903 BbvCI CC'TCA_GC Cuts at: 3536 3536 Size: 6874 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5065 5099 1903 Size: 34 50 34 3044 34 3678 Fragments arranged by size: 3678 3044 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4766 5976 4766 Size: 1210 5664 BclI T'GATC_A Cuts at: 1548 1548 Size: 6874 BfrBI ATG'CAT Cuts at: 3406 3406 Size: 6874 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5485 136 Size: 107 122 71 631 605 3813 1525 Fragments arranged by size: 3813 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2263 6869 2263 Size: 4606 2268 BlpI GC'TnA_GC Cuts at: 2537 3753 3803 4291 2537 Size: 1216 50 488 5120 Fragments arranged by size: 5120 1216 488 50 Bme1580I G_kGCm'C Cuts at: 1790 2718 3535 4935 6091 1790 Size: 928 817 1400 1156 2573 Fragments arranged by size: 2573 1400 1156 928 817 BmgBI CAC'GTC Cuts at: 1318 3152 4081 1318 Size: 1834 929 4111 Fragments arranged by size: 4111 1834 929 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2028 2840 4605 Size: 416 53 83 399 24 611 812 1765 Cuts at: 4605 5563 442 Size: 958 1753 Fragments arranged by size: 1765 1753 958 812 611 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6874 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3532 3532 Size: 6874 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2078 2306 2375 2549 2693 2819 2942 3368 Size: 839 228 69 174 144 126 123 426 Cuts at: 3368 3488 3533 3758 4028 5534 1239 Size: 120 45 225 270 1506 2579 Fragments arranged by size: 2579 1506 839 426 270 228 225 174 144 126 123 120 69 45 Bpu10I CC'TnA_GC Cuts at: 3536 3536 Size: 6874 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2716 4999 5864 6162 2716 Size: 2283 865 298 3428 Fragments arranged by size: 3428 2283 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5537 882 Size: 617 4038 2219 Fragments arranged by size: 4038 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4665 493 Size: 711 3461 2702 Fragments arranged by size: 3461 2702 711 BsaBI GATnn'nnATC Cuts at: 6868 6868 Size: 6874 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2547 2821 3840 4681 Size: 53 83 186 936 1014 274 1019 841 Cuts at: 4681 5063 5698 275 Size: 382 635 1451 Fragments arranged by size: 1451 1019 1014 936 841 635 382 274 186 83 53 BsaWI w'CCGG_w Cuts at: 2019 3873 5307 5705 5979 6126 6537 2019 Size: 1854 1434 398 274 147 411 2356 Fragments arranged by size: 2356 1854 1434 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2732 2762 2897 2927 3146 Size: 30 165 30 1329 30 135 30 219 Cuts at: 3146 3176 3731 3761 1178 Size: 30 555 30 4291 Fragments arranged by size: 4291 1329 555 219 165 135 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2054 2753 2921 3143 3755 1186 Size: 868 699 168 222 612 4305 Fragments arranged by size: 4305 868 699 612 222 168 BseYI C'CCAG_C Cuts at: 2087 2385 2850 3528 4146 6077 6573 2087 Size: 298 465 678 618 1931 496 2388 Fragments arranged by size: 2388 1931 678 618 496 465 298 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4034 1163 Size: 573 2298 4003 Fragments arranged by size: 4003 2298 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4324 5085 6113 1540 Size: 275 186 2323 761 1028 2301 Fragments arranged by size: 2323 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2127 2847 2943 3591 3759 4935 5020 6091 2127 Size: 720 96 648 168 1176 85 1071 2910 Fragments arranged by size: 2910 1176 1071 720 648 168 96 85 BsmI GAATG_Cn' Cuts at: 4385 4478 4385 Size: 93 6781 BsmBI CGTCTCn'nnnn_ Cuts at: 3847 3847 Size: 6874 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2127 2718 2847 2943 3535 3591 3759 Size: 399 337 591 129 96 592 56 168 Cuts at: 3759 3818 4193 4935 5020 6091 1391 Size: 59 375 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 592 591 399 375 337 168 129 96 85 59 56 BspEI T'CCGG_A Cuts at: 2019 3873 6537 2019 Size: 1854 2664 2356 Fragments arranged by size: 2664 2356 1854 BspHI T'CATG_A Cuts at: 4763 6493 4763 Size: 1730 5144 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3771 3807 4127 4229 844 Size: 2927 36 320 102 3489 Fragments arranged by size: 3489 2927 320 102 36 BsrBI CCG'CTC Cuts at: 1394 1823 2011 2965 3992 4761 1394 Size: 429 188 954 1027 769 3507 Fragments arranged by size: 3507 1027 954 769 429 188 BsrDI GCAATG_nn' Cuts at: 65 1248 5369 5543 65 Size: 1183 4121 174 1396 Fragments arranged by size: 4121 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2588 3201 5518 5705 1700 Size: 110 778 613 2317 187 2869 Fragments arranged by size: 2869 2317 778 613 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6874 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6874 BssSI C'ACGA_G Cuts at: 3863 4934 5946 3863 Size: 1071 1012 4791 Fragments arranged by size: 4791 1071 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3178 3763 794 Size: 2384 585 3905 Fragments arranged by size: 3905 2384 585 BstBI TT'CG_AA Cuts at: 2452 2521 5678 2452 Size: 69 3157 3648 Fragments arranged by size: 3648 3157 69 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6874 BstYI r'GATC_y Cuts at: 1972 2263 2487 2886 3018 3339 3867 3906 4204 Size: 291 224 399 132 321 528 39 298 Cuts at: 4204 4571 4956 4973 6425 6511 6523 6869 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 528 399 385 367 346 321 298 291 224 132 86 39 17 12 BtgI C'CryG_G Cuts at: 513 1065 2439 2556 2570 2718 513 Size: 552 1374 117 14 148 4669 Fragments arranged by size: 4669 1374 552 148 117 14 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3371 4126 487 Size: 683 56 2145 755 3235 Fragments arranged by size: 3235 2145 755 683 56 BtsI GCAGTG_nn' Cuts at: 2592 4103 4109 4390 5184 5204 2592 Size: 1511 6 281 794 20 4262 Fragments arranged by size: 4262 1511 794 281 20 6 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6874 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2458 2493 529 Size: 35 1894 35 4910 Fragments arranged by size: 4910 1894 35 35 DraI TTT'AAA Cuts at: 4178 4178 Size: 6874 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5881 809 Size: 507 4565 1802 Fragments arranged by size: 4565 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2553 2598 3267 Size: 54 1425 50 275 18 723 45 669 Cuts at: 3267 3828 4075 4321 5210 6839 8 Size: 561 247 246 889 1629 43 Fragments arranged by size: 1629 1425 889 723 669 561 275 247 246 54 50 45 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4321 1537 Size: 275 2509 4090 Fragments arranged by size: 4090 2509 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2018 2771 3579 3699 4804 1353 Size: 665 753 808 120 1105 3423 Fragments arranged by size: 3423 1105 808 753 665 120 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3154 5461 5835 5981 387 Size: 1064 138 1565 2307 374 146 1280 Fragments arranged by size: 2307 1565 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2125 3589 2125 Size: 1464 5410 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2078 2306 2375 2441 2549 2693 2819 Size: 393 446 228 69 66 108 144 126 Cuts at: 2819 2843 2876 2942 3008 3368 3488 3533 3758 Size: 24 33 66 66 360 120 45 225 Cuts at: 3758 4028 4937 5534 6321 6589 1239 Size: 270 909 597 787 268 1524 Fragments arranged by size: 1524 909 787 597 446 393 360 270 268 228 225 144 126 120 108 69 66 66 66 45 33 24 EcoNI CCTnn'n_nnAGG Cuts at: 3737 3830 3737 Size: 93 6781 EcoO109I rG'GnC_Cy Cuts at: 2929 3532 3823 2929 Size: 603 291 5980 Fragments arranged by size: 5980 603 291 EcoRI G'AATT_C Cuts at: 4183 4561 4183 Size: 378 6496 FspI TGC'GCA Cuts at: 3777 4115 5380 3777 Size: 338 1265 5271 Fragments arranged by size: 5271 1265 338 HaeII r_GCGC'y Cuts at: 1678 2824 2950 3529 3721 4153 6021 1678 Size: 1146 126 579 192 432 1868 2531 Fragments arranged by size: 2531 1868 1146 579 432 192 126 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2497 2529 2714 2746 2897 Size: 32 135 32 925 32 185 32 151 Cuts at: 2897 2929 3146 3178 3482 3514 3643 3675 3731 Size: 32 217 32 304 32 129 32 56 Cuts at: 3731 3763 5515 5547 5589 5621 1373 Size: 32 1752 32 42 32 2626 Fragments arranged by size: 2626 1752 925 304 217 185 151 135 129 56 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4218 4464 669 Size: 988 281 2280 246 3079 Fragments arranged by size: 3079 2280 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6874 HpaI GTT'AAC Cuts at: 4464 4464 Size: 6874 KasI G'GCGC_C Cuts at: 2820 2820 Size: 6874 KpnI G_GTAC'C Cuts at: 4199 4199 Size: 6874 MfeI C'AATT_G Cuts at: 4473 4473 Size: 6874 MluI A'CGCG_T Cuts at: 6750 6750 Size: 6874 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5988 6172 1305 Size: 4683 184 2007 Fragments arranged by size: 4683 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3830 4077 10 Size: 54 1425 343 1998 247 2807 Fragments arranged by size: 2807 1998 1425 343 247 54 MslI CAynn'nnrTG Cuts at: 518 1166 3212 4832 5191 5350 518 Size: 648 2046 1620 359 159 2042 Fragments arranged by size: 2046 2042 1620 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2281 2311 2572 2839 2986 3457 3466 3556 Size: 675 30 261 267 147 471 9 90 Cuts at: 3556 3724 4126 4290 4967 6115 6360 1606 Size: 168 402 164 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 675 471 402 267 261 245 168 164 147 90 30 9 NaeI GCC'GGC Cuts at: 2590 2590 Size: 6874 NarI GG'CG_CC Cuts at: 2821 2821 Size: 6874 NcoI C'CATG_G Cuts at: 513 1065 2556 513 Size: 552 1491 4831 Fragments arranged by size: 4831 1491 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6874 NgoMIV G'CCGG_C Cuts at: 2588 2588 Size: 6874 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6874 NotI GC'GGCC_GC Cuts at: 4321 4321 Size: 6874 NsiI A_TGCA'T Cuts at: 3408 3408 Size: 6874 NspI r_CATG'y Cuts at: 1132 1229 2160 4232 5777 1132 Size: 97 931 2072 1545 2229 Fragments arranged by size: 2229 2072 1545 931 97 PasI CC'CwG_GG Cuts at: 1348 3818 1348 Size: 2470 4404 PciI A'CATG_T Cuts at: 5773 5773 Size: 6874 PflMI CCAn_nnn'nTGG Cuts at: 1108 3727 1108 Size: 2619 4255 PfoI T'CCnGG_A Cuts at: 3504 3591 3735 3882 3504 Size: 87 144 147 6496 Fragments arranged by size: 6496 147 144 87 PmeI GTTT'AAAC Cuts at: 4178 4178 Size: 6874 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2747 2779 4943 4975 6456 6488 2747 Size: 32 2164 32 1481 32 3133 Fragments arranged by size: 3133 2164 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3823 3823 Size: 6874 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6874 PsiI TTA'TAA Cuts at: 4444 4444 Size: 6874 PspOMI G'GGCC_C Cuts at: 2714 3531 2714 Size: 817 6057 PspXI vC'TCGA_Gb Cuts at: 2807 2807 Size: 6874 PstI C_TGCA'G Cuts at: 830 2172 2231 2979 3036 3348 3861 4003 4226 Size: 1342 59 748 57 312 513 142 223 Cuts at: 4226 830 Size: 3478 Fragments arranged by size: 3478 1342 748 513 312 223 142 59 57 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6874 PvuII CAG'CTG Cuts at: 2281 2311 2986 3457 3466 3556 3724 2281 Size: 30 675 471 9 90 168 5431 Fragments arranged by size: 5431 675 471 168 90 30 9 SacI G_AGCT'C Cuts at: 2127 3591 2127 Size: 1464 5410 SacII CC_GC'GG Cuts at: 2573 2573 Size: 6874 SalI G'TCGA_C Cuts at: 1936 4216 1936 Size: 2280 4594 SapI GCTCTTCn'nnn_ Cuts at: 2018 3579 2018 Size: 1561 5313 SbfI CC_TGCA'GG Cuts at: 3348 4226 3348 Size: 878 5996 ScaI AGT'ACT Cuts at: 2657 5122 6834 2657 Size: 2465 1712 2697 Fragments arranged by size: 2697 2465 1712 SfcI C'TryA_G Cuts at: 826 1045 2168 2227 2975 3032 3344 3857 3999 Size: 219 1123 59 748 57 312 513 142 Cuts at: 3999 4222 5357 6038 6229 826 Size: 223 1135 681 191 1471 Fragments arranged by size: 1471 1135 1123 748 681 513 312 223 219 191 142 59 57 SfoI GGC'GCC Cuts at: 2822 2822 Size: 6874 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6874 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6874 SmaI CCC'GGG Cuts at: 1872 4201 1872 Size: 2329 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2731 2807 4978 5879 6141 820 Size: 197 926 788 76 2171 901 262 1553 Fragments arranged by size: 2171 1553 926 901 788 262 197 76 SnaBI TAC'GTA Cuts at: 493 4665 493 Size: 4172 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6874 SphI G_CATG'C Cuts at: 1132 4232 1132 Size: 3100 3774 SspI AAT'ATT Cuts at: 5 52 4798 5 Size: 47 4746 2081 Fragments arranged by size: 4746 2081 47 StuI AGG'CCT Cuts at: 1517 3254 4019 1517 Size: 1737 765 4372 Fragments arranged by size: 4372 1737 765 StyI C'CwwG_G Cuts at: 513 1065 2556 3831 4313 513 Size: 552 1491 1275 482 3074 Fragments arranged by size: 3074 1491 1275 552 482 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2864 3923 4901 4918 5071 5256 5701 Size: 1255 1284 1059 978 17 153 185 445 Cuts at: 5701 325 Size: 1498 Fragments arranged by size: 1498 1284 1255 1059 978 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2655 5120 6832 Size: 274 80 33 51 1443 678 2465 1712 Cuts at: 6832 96 Size: 138 Fragments arranged by size: 2465 1712 1443 678 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2020 2052 2192 2224 2732 2764 6834 6866 2020 Size: 32 140 32 508 32 4070 32 2028 Fragments arranged by size: 4070 2028 508 140 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6874 XbaI T'CTAG_A Cuts at: 2260 4210 2260 Size: 1950 4924 XhoI C'TCGA_G Cuts at: 1943 2807 1943 Size: 864 6010 XmaI C'CCGG_G Cuts at: 1870 4199 1870 Size: 2329 4545 XmnI GAAnn'nnTTC Cuts at: 2759 5003 2759 Size: 2244 4630 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4682 276 Size: 53 83 186 936 3148 2468 Fragments arranged by size: 3148 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI PacI PmlI PsrI RsrII SanDI SexAI SfiI SrfI SwaI XcmI