Restriction map for pFN21AB3300
 (Linear) (Six-base) MAPSORT of: KIBB3300.seq  Check: 6380  from: 1  to: 2169



KIBB3300.orf

 With 182 enzymes: SgfI * 

                        September 18, 2009 17:00  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2122    2169 
   Size:       2122      47
 
AccI GT'mk_AC

Cuts at:      0     557    2169 
   Size:        557    1612
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     436     838     871    1003    2169 
   Size:        436     402      33     132    1166

  Fragments arranged by size: 

                1166     436     402     132      33
 
AfeI AGC'GCT

Cuts at:      0    1522    2169 
   Size:       1522     647
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1491    2169 
   Size:       1491     678
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     178     326    1379    2169 
   Size:        178     148    1053     790

  Fragments arranged by size: 

                1053     790     178     148
 
AlwNI CAG_nnn'CTG

Cuts at:      0     504     927    1023    1176    1293    1338    1551    1846 
   Size:        504     423      96     153     117      45     213     295

Cuts at:   1846    1884    2106    2169 
   Size:         38     222      63

  Fragments arranged by size: 

                 504     423     295     222     213     153     117      96
                  63      45      38
 
ApaI G_GGCC'C

Cuts at:      0     713    1530    2169 
   Size:        713     817     639

  Fragments arranged by size: 

                 817     713     639
 
AvaI C'yCGr_G

Cuts at:      0     692     802    2169 
   Size:        692     110    1367

  Fragments arranged by size: 

                1367     692     110
 
BamHI G'GATC_C

Cuts at:      0    1013    1901    2169 
   Size:       1013     888     268

  Fragments arranged by size: 

                1013     888     268
 
BanI G'GyrC_C

Cuts at:      0     580     815    2169 
   Size:        580     235    1354

  Fragments arranged by size: 

                1354     580     235
 
BanII G_rGCy'C

Cuts at:      0     122     713    1530    1586    1813    2169 
   Size:        122     591     817      56     227     356

  Fragments arranged by size: 

                 817     591     356     227     122      56
 
BbeI G_GCGC'C

Cuts at:      0     819    2169 
   Size:        819    1350
 
BbvCI CC'TCA_GC

Cuts at:      0    1531    2169 
   Size:       1531     638
 
BfrBI ATG'CAT

Cuts at:      0    1401    2169 
   Size:       1401     768
 
BglII A'GATC_T

Cuts at:      0     258    2169 
   Size:        258    1911
 
BlpI GC'TnA_GC

Cuts at:      0     532    1748    1798    2169 
   Size:        532    1216      50     371

  Fragments arranged by size: 

                1216     532     371      50
 
Bme1580I G_kGCm'C

Cuts at:      0     713    1530    2169 
   Size:        713     817     639

  Fragments arranged by size: 

                 817     713     639
 
BmgBI CAC'GTC

Cuts at:      0    1147    2076    2169 
   Size:       1147     929      93

  Fragments arranged by size: 

                1147     929      93
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      23     835    2169 
   Size:         23     812    1334

  Fragments arranged by size: 

                1334     812      23
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1527    2169 
   Size:       1527     642
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     301     370     544     688     814     937    1363 
   Size:         73     228      69     174     144     126     123     426

Cuts at:   1363    1483    1528    1753    2023    2169 
   Size:        120      45     225     270     146

  Fragments arranged by size: 

                 426     270     228     225     174     146     144     126
                 123     120      73      69      45
 
Bpu10I CC'TnA_GC

Cuts at:      0    1531    2169 
   Size:       1531     638
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     711    2169 
   Size:        711    1458
 
BsaHI Gr'CG_yC

Cuts at:      0     542     816    1835    2169 
   Size:        542     274    1019     334

  Fragments arranged by size: 

                1019     542     334     274
 
BsaWI w'CCGG_w

Cuts at:      0      14    1868    2169 
   Size:         14    1854     301

  Fragments arranged by size: 

                1854     301      14
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     727     757     892     922    1141    1171    1726    1756 
   Size:        727      30     135      30     219      30     555      30

Cuts at:   1756    2169 
   Size:        413

  Fragments arranged by size: 

                 727     555     413     219     135      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      49     748     916    1138    1750    2169 
   Size:         49     699     168     222     612     419

  Fragments arranged by size: 

                 699     612     419     222     168      49
 
BseYI C'CCAG_C

Cuts at:      0      82     380     845    1523    2141    2169 
   Size:         82     298     465     678     618      28

  Fragments arranged by size: 

                 678     618     465     298      82      28
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2029    2169 
   Size:       2029     140
 
BsiHKAI G_wGCw'C

Cuts at:      0     122     842     938    1586    1754    2169 
   Size:        122     720      96     648     168     415

  Fragments arranged by size: 

                 720     648     415     168     122      96
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1842    2169 
   Size:       1842     327
 
Bsp1286I G_dGCh'C

Cuts at:      0     122     713     842     938    1530    1586    1754    1813 
   Size:        122     591     129      96     592      56     168      59

Cuts at:   1813    2169 
   Size:        356

  Fragments arranged by size: 

                 592     591     356     168     129     122      96      59
                  56
 
BspEI T'CCGG_A

Cuts at:      0      14    1868    2169 
   Size:         14    1854     301

  Fragments arranged by size: 

                1854     301      14
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1766    1802    2122    2169 
   Size:       1766      36     320      47

  Fragments arranged by size: 

                1766     320      47      36
 
BsrBI CCG'CTC

Cuts at:      0       6     960    1987    2169 
   Size:          6     954    1027     182

  Fragments arranged by size: 

                1027     954     182       6
 
BsrFI r'CCGG_y

Cuts at:      0     583    1196    2169 
   Size:        583     613     973

  Fragments arranged by size: 

                 973     613     583
 
BssSI C'ACGA_G

Cuts at:      0    1858    2169 
   Size:       1858     311
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1173    1758    2169 
   Size:       1173     585     411

  Fragments arranged by size: 

                1173     585     411
 
BstBI TT'CG_AA

Cuts at:      0     447     516    2169 
   Size:        447      69    1653

  Fragments arranged by size: 

                1653     447      69
 
BstYI r'GATC_y

Cuts at:      0     258     482     881    1013    1334    1862    1901    2169 
   Size:        258     224     399     132     321     528      39     268

  Fragments arranged by size: 

                 528     399     321     268     258     224     132      39
            
 
BtgI C'CryG_G

Cuts at:      0     434     551     565     713    2169 
   Size:        434     117      14     148    1456

  Fragments arranged by size: 

                1456     434     148     117      14
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1366    2121    2169 
   Size:       1366     755      48

  Fragments arranged by size: 

                1366     755      48
 
BtsI GCAGTG_nn'

Cuts at:      0     587    2098    2104    2169 
   Size:        587    1511       6      65

  Fragments arranged by size: 

                1511     587      65       6
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     453     488    2169 
   Size:        453      35    1681

  Fragments arranged by size: 

                1681     453      35
 
EaeI y'GGCC_r

Cuts at:      0     548     593    1262    1823    2070    2169 
   Size:        548      45     669     561     247      99

  Fragments arranged by size: 

                 669     561     548     247      99      45
 
EarI CTCTTCn'nnn_

Cuts at:      0      13     766    1574    1694    2169 
   Size:         13     753     808     120     475

  Fragments arranged by size: 

                 808     753     475     120      13
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1149    2169 
   Size:       1149    1020
 
EcoICRI GAG'CTC

Cuts at:      0     120    1584    2169 
   Size:        120    1464     585

  Fragments arranged by size: 

                1464     585     120
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     301     370     436     544     688     814     838 
   Size:         73     228      69      66     108     144     126      24

Cuts at:    838     871     937    1003    1363    1483    1528    1753    2023 
   Size:         33      66      66     360     120      45     225     270

Cuts at:   2023    2169 
   Size:        146

  Fragments arranged by size: 

                 360     270     228     225     146     144     126     120
                 108      73      69      66      66      66      45      33
                  24
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1732    1825    2169 
   Size:       1732      93     344

  Fragments arranged by size: 

                1732     344      93
 
EcoO109I rG'GnC_Cy

Cuts at:      0     924    1527    1818    2169 
   Size:        924     603     291     351

  Fragments arranged by size: 

                 924     603     351     291
 
FspI TGC'GCA

Cuts at:      0    1772    2110    2169 
   Size:       1772     338      59

  Fragments arranged by size: 

                1772     338      59
 
HaeII r_GCGC'y

Cuts at:      0     819     945    1524    1716    2148    2169 
   Size:        819     126     579     192     432      21

  Fragments arranged by size: 

                 819     579     432     192     126      21
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     492     524     709     741     892     924    1141    1173 
   Size:        492      32     185      32     151      32     217      32

Cuts at:   1173    1477    1509    1638    1670    1726    1758    2169 
   Size:        304      32     129      32      56      32     411

  Fragments arranged by size: 

                 492     411     304     217     185     151     129      56
                  32      32      32      32      32      32      32
 
KasI G'GCGC_C

Cuts at:      0     815    2169 
   Size:        815    1354
 
MscI TGG'CCA

Cuts at:      0    1825    2072    2169 
   Size:       1825     247      97

  Fragments arranged by size: 

                1825     247      97
 
MslI CAynn'nnrTG

Cuts at:      0    1207    2169 
   Size:       1207     962
 
MspA1I CmG'CkG

Cuts at:      0     276     306     567     834     981    1452    1461    1551 
   Size:        276      30     261     267     147     471       9      90

Cuts at:   1551    1719    2121    2169 
   Size:        168     402      48

  Fragments arranged by size: 

                 471     402     276     267     261     168     147      90
                  48      30       9
 
NaeI GCC'GGC

Cuts at:      0     585    2169 
   Size:        585    1584
 
NarI GG'CG_CC

Cuts at:      0     816    2169 
   Size:        816    1353
 
NcoI C'CATG_G

Cuts at:      0     551    2169 
   Size:        551    1618
 
NgoMIV G'CCGG_C

Cuts at:      0     583    2169 
   Size:        583    1586
 
NsiI A_TGCA'T

Cuts at:      0    1403    2169 
   Size:       1403     766
 
NspI r_CATG'y

Cuts at:      0     155    2169 
   Size:        155    2014
 
PasI CC'CwG_GG

Cuts at:      0    1813    2169 
   Size:       1813     356
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1722    2169 
   Size:       1722     447
 
PfoI T'CCnGG_A

Cuts at:      0    1499    1586    1730    1877    2169 
   Size:       1499      87     144     147     292

  Fragments arranged by size: 

                1499     292     147     144      87
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     742     774    2169 
   Size:        742      32    1395

  Fragments arranged by size: 

                1395     742      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1818    2169 
   Size:       1818     351
 
PspOMI G'GGCC_C

Cuts at:      0     709    1526    2169 
   Size:        709     817     643

  Fragments arranged by size: 

                 817     709     643
 
PspXI vC'TCGA_Gb

Cuts at:      0     802    2169 
   Size:        802    1367
 
PstI C_TGCA'G

Cuts at:      0     167     226     974    1031    1343    1856    1998    2169 
   Size:        167      59     748      57     312     513     142     171

  Fragments arranged by size: 

                 748     513     312     171     167     142      59      57
            
 
PvuII CAG'CTG

Cuts at:      0     276     306     981    1452    1461    1551    1719    2169 
   Size:        276      30     675     471       9      90     168     450

  Fragments arranged by size: 

                 675     471     450     276     168      90      30       9
            
 
SacI G_AGCT'C

Cuts at:      0     122    1586    2169 
   Size:        122    1464     583

  Fragments arranged by size: 

                1464     583     122
 
SacII CC_GC'GG

Cuts at:      0     568    2169 
   Size:        568    1601
 
SapI GCTCTTCn'nnn_

Cuts at:      0      13    1574    2169 
   Size:         13    1561     595

  Fragments arranged by size: 

                1561     595      13
 
SbfI CC_TGCA'GG

Cuts at:      0    1343    2169 
   Size:       1343     826
 
ScaI AGT'ACT

Cuts at:      0     652    2169 
   Size:        652    1517
 
SfcI C'TryA_G

Cuts at:      0     163     222     970    1027    1339    1852    1994    2169 
   Size:        163      59     748      57     312     513     142     175

  Fragments arranged by size: 

                 748     513     312     175     163     142      59      57
            
 
SfoI GGC'GCC

Cuts at:      0     817    2169 
   Size:        817    1352
 
SmlI C'TyrA_G

Cuts at:      0     726     802    2169 
   Size:        726      76    1367

  Fragments arranged by size: 

                1367     726      76
 
StuI AGG'CCT

Cuts at:      0    1249    2014    2169 
   Size:       1249     765     155

  Fragments arranged by size: 

                1249     765     155
 
StyI C'CwwG_G

Cuts at:      0     551    1826    2169 
   Size:        551    1275     343

  Fragments arranged by size: 

                1275     551     343
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     859    1918    2169 
   Size:        859    1059     251

  Fragments arranged by size: 

                1059     859     251
 
TatI w'GTAC_w

Cuts at:      0     650    2169 
   Size:        650    1519
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      15      47     187     219     727     759    2169 
   Size:         15      32     140      32     508      32    1410

  Fragments arranged by size: 

                1410     508     140      32      32      32      15
 
XbaI T'CTAG_A

Cuts at:      0     255    2169 
   Size:        255    1914
 
XhoI C'TCGA_G

Cuts at:      0     802    2169 
   Size:        802    1367
 
XmnI GAAnn'nnTTC

Cuts at:      0     754    2169 
   Size:        754    1415
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI     AhdI     AlfI    AlwNI     ApaI
     AvaI    BamHI     BanI    BanII     BbeI    BbvCI    BfrBI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsmBI
 Bsp1286I    BspEI    BspMI    BsrBI    BsrFI    BssSI   BstAPI    BstBI
    BstYI     BtgI    BtgZI     BtsI    CspCI     EaeI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I     FspI    HaeII    Hin4I     KasI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NsiI
     NspI     PasI    PflMI     PfoI     PpiI    PpuMI   PspOMI    PspXI
     PstI    PvuII     SacI    SacII     SapI     SbfI     ScaI     SfcI
     SfoI     SmlI     StuI     StyI    TaqII     TatI     TstI     XbaI
     XhoI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII   AflIII     AgeI     AleI     AloI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BbsI
     BcgI    BciVI     BclI     BglI     BmtI     BsaI    BsaAI    BsaBI
    BsiEI    BsiWI     BsmI    BspHI    BsrDI    BsrGI   BssHII   BstEII
    BstXI  BstZ17I   Bsu36I     ClaI     DraI   DraIII     DrdI     EagI
    EcoRI    EcoRV     FalI     FseI    FspAI   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     NdeI     NheI     NotI     NruI
     PacI     PciI     PmeI     PmlI    PshAI     PsiI     PsrI     PvuI
    RsrII     SalI    SanDI    SexAI     SfiI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI  Tth111I     XcmI
     XmaI     ZraI
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