Restriction map for pFN21AB3224
 (Linear) (Six-base) MAPSORT of: KIBB3224.seq  Check: 2782  from: 1  to: 2241



KIBB3224.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:36  ..

AccI GT'mk_AC

Cuts at:      0     202    2241 
   Size:        202    2039
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     433     494     523     701     848    1040    1871    2138 
   Size:        433      61      29     178     147     192     831     267

Cuts at:   2138    2241 
   Size:        103

  Fragments arranged by size: 

                 831     433     267     192     178     147     103      61
                  29
 
AflII C'TTAA_G

Cuts at:      0    1129    2241 
   Size:       1129    1112
 
AflIII A'CryG_T

Cuts at:      0    1054    2241 
   Size:       1054    1187
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1215    2241 
   Size:       1215    1026
 
AleI CACnn'nnGTG

Cuts at:      0      73    2241 
   Size:         73    2168
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1004    2241 
   Size:       1004    1237
 
AlwNI CAG_nnn'CTG

Cuts at:      0      98     331     997    2241 
   Size:         98     233     666    1244

  Fragments arranged by size: 

                1244     666     233      98
 
BamHI G'GATC_C

Cuts at:      0    2007    2241 
   Size:       2007     234
 
BanII G_rGCy'C

Cuts at:      0     856    2241 
   Size:        856    1385
 
BbvCI CC'TCA_GC

Cuts at:      0    1262    2241 
   Size:       1262     979
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     698     732    2241 
   Size:        698      34    1509

  Fragments arranged by size: 

                1509     698      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1044    1666    2241 
   Size:       1044     622     575

  Fragments arranged by size: 

                1044     622     575
 
BclI T'GATC_A

Cuts at:      0     165     948    2241 
   Size:        165     783    1293

  Fragments arranged by size: 

                1293     783     165
 
BfrBI ATG'CAT

Cuts at:      0     838    2241 
   Size:        838    1403
 
BglI GCCn_nnn'nGGC

Cuts at:      0     137    2241 
   Size:        137    2104
 
BlpI GC'TnA_GC

Cuts at:      0    1517    2241 
   Size:       1517     724
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     227     338     444    1756    2241 
   Size:        227     111     106    1312     485

  Fragments arranged by size: 

                1312     485     227     111     106
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76     623     808    1523    2017    2241 
   Size:         76     547     185     715     494     224

  Fragments arranged by size: 

                 715     547     494     224     185      76
 
Bpu10I CC'TnA_GC

Cuts at:      0    1262    2241 
   Size:       1262     979
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1936    2241 
   Size:       1936     305
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     552    1983    2241 
   Size:        552    1431     258

  Fragments arranged by size: 

                1431     552     258
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     549     579    2241 
   Size:        549      30    1662

  Fragments arranged by size: 

                1662     549      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     488     570    2241 
   Size:        488      82    1671

  Fragments arranged by size: 

                1671     488      82
 
BseYI C'CCAG_C

Cuts at:      0     256    1134    2186    2241 
   Size:        256     878    1052      55

  Fragments arranged by size: 

                1052     878     256      55
 
BsmI GAATG_Cn'

Cuts at:      0     721    2241 
   Size:        721    1520
 
Bsp1286I G_dGCh'C

Cuts at:      0     856    2241 
   Size:        856    1385
 
BspHI T'CATG_A

Cuts at:      0    1308    2241 
   Size:       1308     933
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1512    2241 
   Size:       1512     729
 
BsrDI GCAATG_nn'

Cuts at:      0     856    1328    1938    2241 
   Size:        856     472     610     303

  Fragments arranged by size: 

                 856     610     472     303
 
BsrFI r'CCGG_y

Cuts at:      0     960    2241 
   Size:        960    1281
 
BsrGI T'GTAC_A

Cuts at:      0     457    1564    2241 
   Size:        457    1107     677

  Fragments arranged by size: 

                1107     677     457
 
BstEII G'GTnAC_C

Cuts at:      0      75    2241 
   Size:         75    2166
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1878    2241 
   Size:       1878     363
 
BstYI r'GATC_y

Cuts at:      0     756    2007    2241 
   Size:        756    1251     234

  Fragments arranged by size: 

                1251     756     234
 
BstZ17I GTA'TAC

Cuts at:      0     203    2241 
   Size:        203    2038
 
BtgI C'CryG_G

Cuts at:      0     132     782    2241 
   Size:        132     650    1459

  Fragments arranged by size: 

                1459     650     132
 
BtsI GCAGTG_nn'

Cuts at:      0     147    1976    2241 
   Size:        147    1829     265

  Fragments arranged by size: 

                1829     265     147
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1931    1966    2241 
   Size:       1931      35     275

  Fragments arranged by size: 

                1931     275      35
 
DraI TTT'AAA

Cuts at:      0     245    2241 
   Size:        245    1996
 
DraIII CAC_nnn'GTG

Cuts at:      0     221     346    2241 
   Size:        221     125    1895

  Fragments arranged by size: 

                1895     221     125
 
EaeI y'GGCC_r

Cuts at:      0     732    2241 
   Size:        732    1509
 
EarI CTCTTCn'nnn_

Cuts at:      0     971    1676    2241 
   Size:        971     705     565

  Fragments arranged by size: 

                 971     705     565
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76     433     494     523     623     701     808     848 
   Size:         76     357      61      29     100      78     107      40

Cuts at:    848    1040    1523    1871    2017    2138    2241 
   Size:        192     483     348     146     121     103

  Fragments arranged by size: 

                 483     357     348     192     146     121     107     103
                 100      78      76      61      40      29
 
EcoRV GAT'ATC

Cuts at:      0     899    2241 
   Size:        899    1342
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1206    1675    2241 
   Size:       1206     469     566

  Fragments arranged by size: 

                1206     566     469
 
FspI TGC'GCA

Cuts at:      0    1592    2241 
   Size:       1592     649
 
FspAI rTGC'GCAy

Cuts at:      0    1592    2241 
   Size:       1592     649
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      95     127     549     581    1201    1233    1390    1422 
   Size:         95      32     422      32     620      32     157      32

Cuts at:   1422    1640    1672    2241 
   Size:        218      32     569

  Fragments arranged by size: 

                 620     569     422     218     157      95      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0    1767    2241 
   Size:       1767     474
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     245     573    1626    1649    2241 
   Size:        245     328    1053      23     592

  Fragments arranged by size: 

                1053     592     328     245      23
 
MscI TGG'CCA

Cuts at:      0     734    2241 
   Size:        734    1507
 
MslI CAynn'nnrTG

Cuts at:      0      73    2241 
   Size:         73    2168
 
MspA1I CmG'CkG

Cuts at:      0      73      98     134    1261    1849    2241 
   Size:         73      25      36    1127     588     392

  Fragments arranged by size: 

                1127     588     392      73      36      25
 
NcoI C'CATG_G

Cuts at:      0     782    2241 
   Size:        782    1459
 
NdeI CA'TA_TG

Cuts at:      0    1710    2241 
   Size:       1710     531
 
NsiI A_TGCA'T

Cuts at:      0     840    2241 
   Size:        840    1401
 
NspI r_CATG'y

Cuts at:      0     838    1058    1597    1626    2241 
   Size:        838     220     539      29     615

  Fragments arranged by size: 

                 838     615     539     220      29
 
PasI CC'CwG_GG

Cuts at:      0    1434    2241 
   Size:       1434     807
 
PciI A'CATG_T

Cuts at:      0    1054    2241 
   Size:       1054    1187
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     651    1822    2241 
   Size:        651    1171     419

  Fragments arranged by size: 

                1171     651     419
 
PfoI T'CCnGG_A

Cuts at:      0    1378    2241 
   Size:       1378     863
 
PvuII CAG'CTG

Cuts at:      0      98    1261    1849    2241 
   Size:         98    1163     588     392

  Fragments arranged by size: 

                1163     588     392      98
 
SacII CC_GC'GG

Cuts at:      0     135    2241 
   Size:        135    2106
 
SapI GCTCTTCn'nnn_

Cuts at:      0     971    2241 
   Size:        971    1270
 
ScaI AGT'ACT

Cuts at:      0     271    1291    2241 
   Size:        271    1020     950

  Fragments arranged by size: 

                1020     950     271
 
SfcI C'TryA_G

Cuts at:      0     410    1085    2241 
   Size:        410     675    1156

  Fragments arranged by size: 

                1156     675     410
 
SmlI C'TyrA_G

Cuts at:      0    1129    1915    2241 
   Size:       1129     786     326

  Fragments arranged by size: 

                1129     786     326
 
SphI G_CATG'C

Cuts at:      0     838    2241 
   Size:        838    1403
 
SspI AAT'ATT

Cuts at:      0    2164    2241 
   Size:       2164      77
 
StyI C'CwwG_G

Cuts at:      0     746     782    1735    2149    2241 
   Size:        746      36     953     414      92

  Fragments arranged by size: 

                 953     746     414      92      36
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     209     357    1447    1706    1738    2241 
   Size:        209     148    1090     259      32     503

  Fragments arranged by size: 

                1090     503     259     209     148      32
 
TatI w'GTAC_w

Cuts at:      0     158     227     269     457    1289    1564    1685    2241 
   Size:        158      69      42     188     832     275     121     556

  Fragments arranged by size: 

                 832     556     275     188     158     121      69      42
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1566    1598    2241 
   Size:       1566      32     643

  Fragments arranged by size: 

                1566     643      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     563    2241 
   Size:        563    1678
 
XbaI T'CTAG_A

Cuts at:      0     289    2241 
   Size:        289    1952
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     919    2241 
   Size:        919    1322
 
XmnI GAAnn'nnTTC

Cuts at:      0     719    1367    2241 
   Size:        719     648     874

  Fragments arranged by size: 

                 874     719     648
 

 Enzymes that do cut:

     AccI     AcuI    AflII   AflIII     AhdI     AleI     AlfI    AlwNI
    BamHI    BanII    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
     BlpI     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaXI    BseRI
    BseYI     BsmI Bsp1286I    BspHI    BspMI    BsrDI    BsrFI    BsrGI
   BstEII    BstXI    BstYI  BstZ17I     BtgI     BtsI    CspCI     DraI
   DraIII     EaeI     EarI  Eco57MI    EcoRV     FalI     FspI    FspAI
    Hin4I   HincII     MmeI     MscI     MslI   MspA1I     NcoI     NdeI
     NsiI     NspI     PasI     PciI    PflMI     PfoI    PvuII    SacII
     SapI     ScaI     SfcI     SmlI     SphI     SspI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI     AgeI     AloI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
     BanI     BbeI     BbsI    BglII Bme1580I    BmgBI     BmtI     BplI
    BsaAI    BsaBI    BsaHI    BsaWI     BsgI    BsiEI  BsiHKAI    BsiWI
    BsmBI    BspEI    BsrBI   BssHII    BssSI   BstAPI    BstBI   Bsu36I
    BtgZI     ClaI     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI     FseI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     NaeI     NarI   NgoMIV     NheI     NotI     NruI     PacI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI     SalI    SanDI     SbfI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SrfI     StuI     SwaI     XhoI     XmaI     ZraI
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