Restriction map for
pFN21AB3201
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AgeI
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp718I AspI
AvaII AviII AvrII BanI BbeI
BbrPI BbvCI BcgI BciVI BcnI
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BseRI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I Cac8I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DsaI EagI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FauI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HphI KasI
KpnI KspI MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SwaI Tth111I XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AciI 173 CCGC CCGC(-3/-1)
AflII 177 CTTAAG C^TTAAG
AhdI 10 GACGTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 862 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 536 GGGCCC GGGCC^C
Asp700I 876 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 10 GACGTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 842 GTGCTC G[AT]GC[AT]^C
BalI 473 TGGCCA TGG^CCA
BamHI 395 GGATCC G^GATCC
BbsI 725 GTCTTC GAAGAC(2/6)
BclI 657 TGATCA T^GATCA
BfrI 177 CTTAAG C^TTAAG
BglI 856 GCCACACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 807 AGATCT A^GATCT
BpuAI 725 GTCTTC GAAGAC(2/6)
BsaI 226 GGTCTC GGTCTC(1/5)
BsgI 785 GTGCAG GTGCAG(16/14)
BsiHKAI 842 GTGCTC G[AT]GC[AT]^C
BsmBI 794 GAGACG CGTCTC(1/5)
BsmFI 60 GGGAC GGGAC(10/14)
DraII 536 GGGCCCC [GA]G^G[ACGT]CC[CT]
DrdI 926 GACCAAACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 10 GACGTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 362 CTCTTC CTCTTC(1/4)
EcoNI 51 CCTTGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 536 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 51 CCTTGG C^C[AT][AT]GG
FbaI 657 TGATCA T^GATCA
HapII 712 CCGG C^CGG
HpaII 712 CCGG C^CGG
Ksp632I 362 CTCTTC CTCTTC(1/4)
MaeII 11 ACGT A^CGT
MluNI 473 TGGCCA TGG^CCA
MscI 473 TGGCCA TGG^CCA
MspA1I 916 CAGCTG C[AC]G^C[GT]G
MspI 712 CCGG C^CGG
MwoI 856 GCCACACAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflMI 901 CCAACACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 360 GACTC GAGTC(4/5)
PvuII 916 CAGCTG CAG^CTG
SexAI 523 ACCAGGT A^CC[AT]GGT
SmlI 177 CTTAAG C^T[CT][GA]AG
StuI 998 AGGCCT AGG^CCT
StyI 51 CCTTGG C^C[AT][AT]GG
TaqI 846 TCGA T^CGA
TfiI 646 GATTC G^A[AT]TC
Tsp45I 739 GTGAC ^GT[GC]AC
TthHB8I 846 TCGA T^CGA
Van91I 901 CCAACACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 291 TCTAGA T^CTAGA
XmnI 876 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 11 enzymes) #
###################################
AvaI 20 CTCGGG C^[CT]CG[GA]G
541 CCCGAG
BanII 489 GAGCCC G[GA]GC[CT]^C
536 GGGCCC
BbvI 787 GCAGC GCAGC(8/12)
918 GCTGC
BsoBI 20 CTCGGG C^[CT]CG[GA]G
541 CCCGAG
BsrI 456 ACTGG ACTGG(1/-1)
937 CCAGT
Fnu4HI 787 GCAGC GC^[ACGT]GC
918 GCTGC
HinfI 360 GACTC G^A[ACGT]TC
646 GATTC
ItaI 787 GCAGC GC^[ACGT]GC
918 GCTGC
MslI 351 CATGTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
905 CACCTGGATG
SfaNI 235 GATGC GCATC(5/9)
771 GCATC
TseI 787 GCAGC G^C[AT]GC
918 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AflIII AluI AlwI BfaI BmyI
BsaJI BsiYI BslI BsmAI Bsp1286I
BspLU11I BssKI BstF5I BstNI BstYI
Cfr13I DdeI DpnII EaeI EcoRII
FokI HaeIII MaeI MaeIII MboI
MboII MflI MnlI MseI MvaI
NdeII NlaIII NlaIV NspI Sau3AI
Sau96I ScrFI SspI Tru9I Tsp509I
TspRI XhoII