Restriction map for
pFN21AB3201
(Linear) (Six-base) MAPSORT of: KIBB3201.seq Check: 1171 from: 1 to: 1038
KIBB3201.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 765 1038
Size: 765 273
AflII C'TTAA_G
Cuts at: 0 177 1038
Size: 177 861
AflIII A'CryG_T
Cuts at: 0 350 584 716 1038
Size: 350 234 132 322
Fragments arranged by size:
350 322 234 132
AhdI GACnn_n'nnGTC
Cuts at: 0 15 1038
Size: 15 1023
AlwNI CAG_nnn'CTG
Cuts at: 0 867 1038
Size: 867 171
ApaI G_GGCC'C
Cuts at: 0 540 1038
Size: 540 498
AvaI C'yCGr_G
Cuts at: 0 20 541 1038
Size: 20 521 497
Fragments arranged by size:
521 497 20
BamHI G'GATC_C
Cuts at: 0 395 1038
Size: 395 643
BanII G_rGCy'C
Cuts at: 0 493 540 1038
Size: 493 47 498
Fragments arranged by size:
498 493 47
BbsI GAAGACnn'nnnn_
Cuts at: 0 718 1038
Size: 718 320
BclI T'GATC_A
Cuts at: 0 657 1038
Size: 657 381
BglI GCCn_nnn'nGGC
Cuts at: 0 862 1038
Size: 862 176
BglII A'GATC_T
Cuts at: 0 807 1038
Size: 807 231
Bme1580I G_kGCm'C
Cuts at: 0 540 691 1038
Size: 540 151 347
Fragments arranged by size:
540 347 151
BmgBI CAC'GTC
Cuts at: 0 12 1038
Size: 12 1026
BsaI GGTCTCn'nnnn_
Cuts at: 0 232 1038
Size: 232 806
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 49 79 1038
Size: 49 30 959
Fragments arranged by size:
959 49 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 806 1038
Size: 806 232
BsiHKAI G_wGCw'C
Cuts at: 0 846 1038
Size: 846 192
BsmBI CGTCTCn'nnnn_
Cuts at: 0 788 1038
Size: 788 250
Bsp1286I G_dGCh'C
Cuts at: 0 493 540 691 846 1038
Size: 493 47 151 155 192
Fragments arranged by size:
493 192 155 151 47
BstYI r'GATC_y
Cuts at: 0 395 408 807 1038
Size: 395 13 399 231
Fragments arranged by size:
399 395 231 13
DrdI GACnn_nn'nnGTC
Cuts at: 0 932 1038
Size: 932 106
EaeI y'GGCC_r
Cuts at: 0 473 709 854 892 1038
Size: 473 236 145 38 146
Fragments arranged by size:
473 236 146 145 38
EarI CTCTTCn'nnn_
Cuts at: 0 368 1038
Size: 368 670
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 765 1038
Size: 765 273
EcoNI CCTnn'n_nnAGG
Cuts at: 0 55 1038
Size: 55 983
EcoO109I rG'GnC_Cy
Cuts at: 0 537 1038
Size: 537 501
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 63 647 1038
Size: 63 584 391
Fragments arranged by size:
584 391 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1 33 1038
Size: 1 32 1005
Fragments arranged by size:
1005 32 1
MscI TGG'CCA
Cuts at: 0 475 1038
Size: 475 563
MslI CAynn'nnrTG
Cuts at: 0 355 909 1038
Size: 355 554 129
Fragments arranged by size:
554 355 129
MspA1I CmG'CkG
Cuts at: 0 918 1038
Size: 918 120
NspI r_CATG'y
Cuts at: 0 354 588 720 1038
Size: 354 234 132 318
Fragments arranged by size:
354 318 234 132
PciI A'CATG_T
Cuts at: 0 350 584 716 1038
Size: 350 234 132 322
Fragments arranged by size:
350 322 234 132
PflMI CCAn_nnn'nTGG
Cuts at: 0 907 1038
Size: 907 131
PsiI TTA'TAA
Cuts at: 0 301 1038
Size: 301 737
PspOMI G'GGCC_C
Cuts at: 0 536 1038
Size: 536 502
PvuII CAG'CTG
Cuts at: 0 918 1038
Size: 918 120
SexAI A'CCwGG_T
Cuts at: 0 523 1038
Size: 523 515
SmlI C'TyrA_G
Cuts at: 0 177 1038
Size: 177 861
SspI AAT'ATT
Cuts at: 0 258 379 674 1038
Size: 258 121 295 364
Fragments arranged by size:
364 295 258 121
StuI AGG'CCT
Cuts at: 0 1000 1038
Size: 1000 38
StyI C'CwwG_G
Cuts at: 0 51 1038
Size: 51 987
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 555 587 1038
Size: 555 32 451
Fragments arranged by size:
555 451 32
XbaI T'CTAG_A
Cuts at: 0 291 1038
Size: 291 747
XmnI GAAnn'nnTTC
Cuts at: 0 880 1038
Size: 880 158
Enzymes that do cut:
AcuI AflII AflIII AhdI AlwNI ApaI AvaI BamHI
BanII BbsI BclI BglI BglII Bme1580I BmgBI BsaI
BsaXI BsgI BsiHKAI BsmBI Bsp1286I BstYI DrdI EaeI
EarI Eco57MI EcoNI EcoO109I FalI Hin4I MscI MslI
MspA1I NspI PciI PflMI PsiI PspOMI PvuII SexAI
SmlI SspI StuI StyI TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BanI BbeI BbvCI BcgI BciVI BfrBI BlpI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiWI BsmI BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII EagI EciI EcoICRI
EcoRI EcoRV FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PfoI PmeI PmlI PpiI PpuMI PshAI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SwaI TaqII TatI
Tth111I XcmI XhoI XmaI ZraI