Restriction map for
pFN21AB3039
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# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccI AclI AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BbrPI BcgI BlnI BsaI BsiEI
BsiWI BspDI BspLU11I BsrBI BsrFI
BssSI Bst1107I BstAPI BstBI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoRI EcoRV
FseI FspI HpaI KpnI KspI
MluI NaeI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I StuI SwaI Tth111I
XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccIII 623 TCCGGA T^CCGGA
AcyI 146 GGCGCC G[GA]^CG[CT]C
AflII 2515 CTTAAG C^TTAAG
AlwNI 1283 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 1174 GTGCTC G[AT]GC[AT]^C
BbeI 146 GGCGCC GGCGC^C
BbsI 2041 GAAGAC GAAGAC(2/6)
BbvCI 1958 CCTCAGC CCTCAGC(-5/-2)
BciVI 335 GGATAC GTATCC(6/5)
BfrI 2515 CTTAAG C^TTAAG
BglI 2836 GCCGACGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1881 AGATCT A^GATCT
BlpI 541 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 541 GCTCAGC GC^T[ACGT]AGC
BpuAI 2041 GAAGAC GAAGAC(2/6)
BsaHI 146 GGCGCC G[GA]^CG[CT]C
BseAI 623 TCCGGA T^CCGGA
BsiHKAI 1174 GTGCTC G[AT]GC[AT]^C
Bsp1407I 3041 TGTACA T^GTACA
BspEI 623 TCCGGA T^CCGGA
BspHI 2178 TCATGA T^CATGA
BsrGI 3041 TGTACA T^GTACA
BssHII 2521 GCGCGC G^CGCGC
BstEII 2187 GGTGACC G^GT[ACGT]ACC
BstPI 2187 GGTGACC G^GT[ACGT]ACC
CelII 541 GCTCAGC GC^T[ACGT]AGC
EcoO65I 2187 GGTGACC G^GT[ACGT]ACC
EcoT22I 717 ATGCAT ATGCA^T
HaeII 146 GGCGCC [GA]GCGC^[CT]
Hin1I 146 GGCGCC G[GA]^CG[CT]C
KasI 146 GGCGCC G^GCGCC
MfeI 2311 CAATTG C^AATTG
MroI 623 TCCGGA T^CCGGA
MunI 2311 CAATTG C^AATTG
NarI 146 GGCGCC GG^CGCC
NsiI 717 ATGCAT ATGCA^T
PpuMI 648 GGGACCT [GA]G^G[AT]CC[CT]
RcaI 2178 TCATGA T^CATGA
SexAI 2123 ACCAGGT A^CC[AT]GGT
SfoI 146 GGCGCC GGC^GCC
SmaI 2846 CCCGGG CCC^GGG
SphI 901 GCATGC GCATG^C
SspBI 3041 TGTACA T^GTACA
TfiI 2200 GAATC G^A[AT]TC
XbaI 519 TCTAGA T^CTAGA
XmaCI 2846 CCCGGG C^CCGGG
XmaI 2846 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 748 ACGTGT A^C[GA][CT]GT
773 ACACGT
AvaI 2846 CCCGGG C^[CT]CG[GA]G
2957 CCCGAG
BamHI 207 GGATCC G^GATCC
1238 GGATCC
BanI 64 GGTGCC G^G[CT][GA]CC
146 GGCGCC
BanII 157 GAGCCC G[GA]GC[CT]^C
1009 GGGCTC
BsaAI 1831 TACGTG [CT]AC^GT[GA]
3304 TACGTG
BsaBI 78 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3012 GATGGCCATC
BsaWI 623 TCCGGA [AT]^CCGG[AT]
1235 ACCGGA
BsmBI 1599 GAGACG CGTCTC(1/5)
2761 GAGACG
BsoBI 2846 CCCGGG C^[CT]CG[GA]G
2957 CCCGAG
BstXI 549 CCAGGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3001 CCAGTGCGCTGG
DraII 648 GGGACCT [GA]G^G[ACGT]CC[CT]
2099 AGGGCCT
DsaI 68 CCATGG C^C[GA][CT]GG
2718 CCATGG
EcoO109I 648 GGGACCT [GA]G^G[ACGT]CC[CT]
2099 AGGGCCT
FauI 3119 GCGGG CCCGC(4/6)
3225 GCGGG
HincII 356 GTCAAC GT[CT]^[GA]AC
1849 GTCAAC
HindII 356 GTCAAC GT[CT]^[GA]AC
1849 GTCAAC
MamI 78 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3012 GATGGCCATC
MslI 345 CATGAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2050 CACGTTGATG
NcoI 68 CCATGG C^CATGG
2718 CCATGG
NspI 901 GCATGC [GA]CATG^[CT]
2671 GCATGT
PstI 886 CTGCAG CTGCA^G
1269 CTGCAG
XcmI 699 CCATGAAGATACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2449 CCAGTGCTTGACTGG
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# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AvaII BalI BbvI
BclI BcnI BfaI BmyI BpmI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII EaeI EarI EcoNI
EcoRII EcoT14I FbaI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII