Restriction map for
pFN21AB3039
(Linear) (Six-base) MAPSORT of: KIBB3039.seq Check: 3088 from: 1 to: 3372
KIBB3039.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 136 539 3372
Size: 136 403 2833
Fragments arranged by size:
2833 403 136
AflII C'TTAA_G
Cuts at: 0 2515 3372
Size: 2515 857
AflIII A'CryG_T
Cuts at: 0 748 773 3372
Size: 748 25 2599
Fragments arranged by size:
2599 748 25
AlwNI CAG_nnn'CTG
Cuts at: 0 1288 3372
Size: 1288 2084
ApoI r'AATT_y
Cuts at: 0 1093 1677 2473 3372
Size: 1093 584 796 899
Fragments arranged by size:
1093 899 796 584
AvaI C'yCGr_G
Cuts at: 0 2846 2957 3372
Size: 2846 111 415
Fragments arranged by size:
2846 415 111
BamHI G'GATC_C
Cuts at: 0 207 1238 3372
Size: 207 1031 2134
Fragments arranged by size:
2134 1031 207
BanI G'GyrC_C
Cuts at: 0 64 146 3372
Size: 64 82 3226
Fragments arranged by size:
3226 82 64
BanII G_rGCy'C
Cuts at: 0 161 1013 3372
Size: 161 852 2359
Fragments arranged by size:
2359 852 161
BbeI G_GCGC'C
Cuts at: 0 150 3372
Size: 150 3222
BbsI GAAGACnn'nnnn_
Cuts at: 0 2048 3372
Size: 2048 1324
BbvCI CC'TCA_GC
Cuts at: 0 1959 3372
Size: 1959 1413
BciVI GTATCCnnnnn_n'
Cuts at: 0 329 3372
Size: 329 3043
BclI T'GATC_A
Cuts at: 0 83 1758 2300 2592 3372
Size: 83 1675 542 292 780
Fragments arranged by size:
1675 780 542 292 83
BfrBI ATG'CAT
Cuts at: 0 719 3372
Size: 719 2653
BglI GCCn_nnn'nGGC
Cuts at: 0 2842 3372
Size: 2842 530
BglII A'GATC_T
Cuts at: 0 1881 3372
Size: 1881 1491
BlpI GC'TnA_GC
Cuts at: 0 542 3372
Size: 542 2830
Bme1580I G_kGCm'C
Cuts at: 0 497 3372
Size: 497 2875
BmgBI CAC'GTC
Cuts at: 0 749 2841 3372
Size: 749 2092 531
Fragments arranged by size:
2092 749 531
BmrI ACTGGGnnnn_n'
Cuts at: 0 2995 3372
Size: 2995 377
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 607 3372
Size: 607 2765
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 578 731 980 997 2779 3190 3372
Size: 578 153 249 17 1782 411 182
Fragments arranged by size:
1782 578 411 249 182 153 17
Bpu10I CC'TnA_GC
Cuts at: 0 937 1959 3099 3372
Size: 937 1022 1140 273
Fragments arranged by size:
1140 1022 937 273
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 2080 2189 2883 3372
Size: 209 1871 109 694 489
Fragments arranged by size:
1871 694 489 209 109
BsaAI yAC'GTr
Cuts at: 0 1833 3306 3372
Size: 1833 1473 66
Fragments arranged by size:
1833 1473 66
BsaBI GATnn'nnATC
Cuts at: 0 82 3016 3372
Size: 82 2934 356
Fragments arranged by size:
2934 356 82
BsaHI Gr'CG_yC
Cuts at: 0 147 3372
Size: 147 3225
BsaWI w'CCGG_w
Cuts at: 0 623 1235 3372
Size: 623 612 2137
Fragments arranged by size:
2137 623 612
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 398 428 1250 1280 1726 1756 3372
Size: 398 30 822 30 446 30 1616
Fragments arranged by size:
1616 822 446 398 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 335 1622 1907 1947 3304 3372
Size: 335 1287 285 40 1357 68
Fragments arranged by size:
1357 1287 335 285 68 40
BseYI C'CCAG_C
Cuts at: 0 1288 2166 2769 2813 3372
Size: 1288 878 603 44 559
Fragments arranged by size:
1288 878 603 559 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 754 822 963 2401 3152 3372
Size: 754 68 141 1438 751 220
Fragments arranged by size:
1438 754 751 220 141 68
BsiHKAI G_wGCw'C
Cuts at: 0 1178 3372
Size: 1178 2194
BsmI GAATG_Cn'
Cuts at: 0 661 721 1350 2076 3372
Size: 661 60 629 726 1296
Fragments arranged by size:
1296 726 661 629 60
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1593 2755 3372
Size: 1593 1162 617
Fragments arranged by size:
1593 1162 617
Bsp1286I G_dGCh'C
Cuts at: 0 161 497 1013 1178 3372
Size: 161 336 516 165 2194
Fragments arranged by size:
2194 516 336 165 161
BspEI T'CCGG_A
Cuts at: 0 623 3372
Size: 623 2749
BspHI T'CATG_A
Cuts at: 0 2178 3372
Size: 2178 1194
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 136 539 2281 3372
Size: 136 403 1742 1091
Fragments arranged by size:
1742 1091 403 136
BsrDI GCAATG_nn'
Cuts at: 0 131 900 2780 3372
Size: 131 769 1880 592
Fragments arranged by size:
1880 769 592 131
BsrGI T'GTAC_A
Cuts at: 0 3041 3372
Size: 3041 331
BssHII G'CGCG_C
Cuts at: 0 2521 3372
Size: 2521 851
BstEII G'GTnAC_C
Cuts at: 0 2187 3372
Size: 2187 1185
BstXI CCAn_nnnn'nTGG
Cuts at: 0 556 3008 3372
Size: 556 2452 364
Fragments arranged by size:
2452 556 364
BstYI r'GATC_y
Cuts at: 0 207 1238 1881 2433 2889 3372
Size: 207 1031 643 552 456 483
Fragments arranged by size:
1031 643 552 483 456 207
BtgI C'CryG_G
Cuts at: 0 68 2718 3372
Size: 68 2650 654
Fragments arranged by size:
2650 654 68
BtsI GCAGTG_nn'
Cuts at: 0 464 810 895 1278 1886 3372
Size: 464 346 85 383 608 1486
Fragments arranged by size:
1486 608 464 383 346 85
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1037 1072 3372
Size: 1037 35 2300
Fragments arranged by size:
2300 1037 35
EaeI y'GGCC_r
Cuts at: 0 569 1123 2505 2699 3014 3372
Size: 569 554 1382 194 315 358
Fragments arranged by size:
1382 569 554 358 315 194
EarI CTCTTCn'nnn_
Cuts at: 0 259 598 2311 3372
Size: 259 339 1713 1061
Fragments arranged by size:
1713 1061 339 259
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 578 731 980 997 2779 3190 3372
Size: 578 153 249 17 1782 411 182
Fragments arranged by size:
1782 578 411 249 182 153 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 609 1253 1637 3372
Size: 609 644 384 1735
Fragments arranged by size:
1735 644 609 384
EcoO109I rG'GnC_Cy
Cuts at: 0 649 2100 3372
Size: 649 1451 1272
Fragments arranged by size:
1451 1272 649
HaeII r_GCGC'y
Cuts at: 0 150 3372
Size: 150 3222
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 314 346 954 986 1195 1227 1250 1282
Size: 314 32 608 32 209 32 23 32
Cuts at: 1282 1726 1758 1832 1864 1866 1898 3049 3081
Size: 444 32 74 32 2 32 1151 32
Cuts at: 3081 3232 3264 3372
Size: 151 32 108
Fragments arranged by size:
1151 608 444 314 209 151 108 74
32 32 32 32 32 32 32 32
32 23 2
HincII GTy'rAC
Cuts at: 0 358 1851 3372
Size: 358 1493 1521
Fragments arranged by size:
1521 1493 358
HindIII A'AGCT_T
Cuts at: 0 362 934 1112 1438 3372
Size: 362 572 178 326 1934
Fragments arranged by size:
1934 572 362 326 178
KasI G'GCGC_C
Cuts at: 0 146 3372
Size: 146 3226
MfeI C'AATT_G
Cuts at: 0 2311 3372
Size: 2311 1061
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1967 3372
Size: 1967 1405
MscI TGG'CCA
Cuts at: 0 2507 2701 3016 3372
Size: 2507 194 315 356
Fragments arranged by size:
2507 356 315 194
MslI CAynn'nnrTG
Cuts at: 0 349 2054 3372
Size: 349 1705 1318
Fragments arranged by size:
1705 1318 349
MspA1I CmG'CkG
Cuts at: 0 213 947 1963 2215 2377 3372
Size: 213 734 1016 252 162 995
Fragments arranged by size:
1016 995 734 252 213 162
NarI GG'CG_CC
Cuts at: 0 147 3372
Size: 147 3225
NcoI C'CATG_G
Cuts at: 0 68 2718 3372
Size: 68 2650 654
Fragments arranged by size:
2650 654 68
NsiI A_TGCA'T
Cuts at: 0 721 3372
Size: 721 2651
NspI r_CATG'y
Cuts at: 0 905 2675 3372
Size: 905 1770 697
Fragments arranged by size:
1770 905 697
PasI CC'CwG_GG
Cuts at: 0 1820 1863 3156 3372
Size: 1820 43 1293 216
Fragments arranged by size:
1820 1293 216 43
PflMI CCAn_nnn'nTGG
Cuts at: 0 242 875 3264 3372
Size: 242 633 2389 108
Fragments arranged by size:
2389 633 242 108
PfoI T'CCnGG_A
Cuts at: 0 996 1587 3372
Size: 996 591 1785
Fragments arranged by size:
1785 996 591
PpuMI rG'GwC_Cy
Cuts at: 0 649 3372
Size: 649 2723
PstI C_TGCA'G
Cuts at: 0 890 1273 3372
Size: 890 383 2099
Fragments arranged by size:
2099 890 383
PvuII CAG'CTG
Cuts at: 0 947 1963 2377 3372
Size: 947 1016 414 995
Fragments arranged by size:
1016 995 947 414
SexAI A'CCwGG_T
Cuts at: 0 2123 3372
Size: 2123 1249
SfcI C'TryA_G
Cuts at: 0 886 1269 3162 3372
Size: 886 383 1893 210
Fragments arranged by size:
1893 886 383 210
SfoI GGC'GCC
Cuts at: 0 148 3372
Size: 148 3224
SmaI CCC'GGG
Cuts at: 0 2848 3372
Size: 2848 524
SmlI C'TyrA_G
Cuts at: 0 224 2095 2204 2515 2862 3372
Size: 224 1871 109 311 347 510
Fragments arranged by size:
1871 510 347 311 224 109
SphI G_CATG'C
Cuts at: 0 905 3372
Size: 905 2467
SspI AAT'ATT
Cuts at: 0 1697 1770 1982 3372
Size: 1697 73 212 1390
Fragments arranged by size:
1697 1390 212 73
StyI C'CwwG_G
Cuts at: 0 68 978 1856 2253 2718 3372
Size: 68 910 878 397 465 654
Fragments arranged by size:
910 878 654 465 397 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 131 845 3372
Size: 131 714 2527
Fragments arranged by size:
2527 714 131
TatI w'GTAC_w
Cuts at: 0 525 563 670 3041 3372
Size: 525 38 107 2371 331
Fragments arranged by size:
2371 525 331 107 38
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 398 430 1887 1919 3114 3146 3372
Size: 398 32 1457 32 1195 32 226
Fragments arranged by size:
1457 1195 398 226 32 32 32
XbaI T'CTAG_A
Cuts at: 0 519 3372
Size: 519 2853
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 706 2456 3372
Size: 706 1750 916
Fragments arranged by size:
1750 916 706
XmaI C'CCGG_G
Cuts at: 0 2846 3372
Size: 2846 526
Enzymes that do cut:
AarI AflII AflIII AlwNI ApoI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrDI BsrGI BssHII BstEII BstXI BstYI BtgI BtsI
CspCI EaeI EarI Eco57MI EcoNI EcoO109I HaeII Hin4I
HincII HindIII KasI MfeI MmeI MscI MslI MspA1I
NarI NcoI NsiI NspI PasI PflMI PfoI PpuMI
PstI PvuII SexAI SfcI SfoI SmaI SmlI SphI
SspI StyI TaqII TatI TstI XbaI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BcgI BmtI BsaI BsiEI BsiWI BsrBI
BsrFI BssSI BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI
DraI DraIII DrdI EagI EciI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HpaI KpnI MluI NaeI
NdeI NgoMIV NheI NotI NruI PacI PciI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfiI SgfI SgrAI SnaBI SpeI SrfI StuI SwaI
Tth111I XhoI XmnI ZraI