Restriction map for
pFN21AB1338
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# NO CUT ENZYMES ( 94 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AgeI ApoI AscI AseI
AsnI Asp718I AspI AvrII BcgI
BciVI BclI BfrI BglII BlnI
BsaBI BsaWI BseAI BsiWI BsmI
BspDI BspEI BspHI BspLU11I BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I ClaI CpoI DraI
EagI EclXI Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HindIII
HpaI KpnI MamI MfeI MluI
MroI MseI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SapI SbfI ScaI
SfiI SfuI SnaBI SpeI Sse8387I
SspI StuI SwaI Tru9I Tsp509I
Tth111I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 615 GACGTC GACGT^C
AflIII 218 ACGTGT A^C[GA][CT]GT
AhdI 949 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 40 GTGCAC G^TGCAC
AocI 968 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1510 AGCGCT AGC^GCT
ApaLI 40 GTGCAC G^TGCAC
Asp700I 1488 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 949 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1574 TGCGCA TGC^GCA
BalI 716 TGGCCA TGG^CCA
BamHI 925 GGATCC G^GATCC
BbrPI 217 CACGTG CAC^GTG
BbsI 1711 GTCTTC GAAGAC(2/6)
BbvCI 1424 CCTCAGC CCTCAGC(-5/-2)
BpuAI 1711 GTCTTC GAAGAC(2/6)
BsaAI 217 CACGTG [CT]AC^GT[GA]
BsgI 725 GTGCAG GTGCAG(16/14)
BsiEI 336 CGACCG CG[GA][CT]^CG
BsmBI 1546 GAGACG CGTCTC(1/5)
Bsp1407I 221 TGTACA T^GTACA
BsrGI 221 TGTACA T^GTACA
Bsu36I 968 CCTCAGG CC^T[ACGT]AGG
DraIII 1163 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 716 TGGCCA [CT]^GGCC[GA]
Eam1105I 949 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1510 AGCGCT AGC^GCT
Eco81I 968 CCTCAGG CC^T[ACGT]AGG
FspI 1574 TGCGCA TGC^GCA
KspI 1774 CCGCGG CCGC^GG
MaeIII 573 GTCAC ^GT[ACGT]AC
MluNI 716 TGGCCA TGG^CCA
MscI 716 TGGCCA TGG^CCA
MslI 168 CATGGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1587 GCCGGC GCC^GGC
NgoMIV 1587 GCCGGC G^CCGGC
NspI 173 GCATGC [GA]CATG^[CT]
PmaCI 217 CACGTG CAC^GTG
PmlI 217 CACGTG CAC^GTG
SacI 1761 GAGCTC GAGCT^C
SacII 1774 CCGCGG CCGC^GG
SexAI 1164 ACCTGGT A^CC[AT]GGT
SgrAI 1586 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 536 CCCGGG CCC^GGG
SphI 173 GCATGC GCATG^C
SspBI 221 TGTACA T^GTACA
TfiI 1207 GAATC G^A[AT]TC
Tsp45I 573 GTCAC ^GT[GC]AC
XmaCI 536 CCCGGG C^CCGGG
XmaI 536 CCCGGG C^CCGGG
XmnI 1488 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwNI 1237 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1741 CAGCCGCTG
AspHI 40 GTGCAC G[AT]GC[AT]^C
1761 GAGCTC
BbeI 20 GGCGCC GGCGC^C
1620 GGCGCC
BfaI 107 CTAG C^TAG
1354 CTAG
BlpI 1665 GCTCAGC GC^T[ACGT]AGC
1763 GCTCAGC
Bpu1102I 1665 GCTCAGC GC^T[ACGT]AGC
1763 GCTCAGC
BsiHKAI 40 GTGCAC G[AT]GC[AT]^C
1761 GAGCTC
BsrDI 236 CATTGC GCAATG(2/0)
86 GCAATG
BstAPI 1236 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1740 GCAGCCGCTGC
BstYI 925 GGATCC [GA]^GATC[CT]
1002 GGATCT
CelII 1665 GCTCAGC GC^T[ACGT]AGC
1763 GCTCAGC
HincII 361 GTCAAC GT[CT]^[GA]AC
1441 GTCAAC
HindII 361 GTCAAC GT[CT]^[GA]AC
1441 GTCAAC
KasI 20 GGCGCC G^GCGCC
1620 GGCGCC
MaeI 107 CTAG C^TAG
1354 CTAG
MflI 925 GGATCC [GA]^GATC[CT]
1002 GGATCT
NarI 20 GGCGCC GG^CGCC
1620 GGCGCC
NcoI 713 CCATGG C^CATGG
883 CCATGG
SfoI 20 GGCGCC GGC^GCC
1620 GGCGCC
TaqI 335 TCGA T^CGA
1177 TCGA
TthHB8I 335 TCGA T^CGA
1177 TCGA
XhoII 925 GGATCC [GA]^GATC[CT]
1002 GGATCT
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# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AvaI AvaII BanI
BanII BbvI BcnI BglI BmyI
BpmI BsaHI BsaI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DrdI DsaI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TseI TspRI
XcmI