Restriction map for
pFN21AB1338
(Linear) (Six-base) MAPSORT of: KIBB1338.seq Check: 4209 from: 1 to: 1815
KIBB1338.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 375 1815
Size: 375 1440
AatII G_ACGT'C
Cuts at: 0 619 1815
Size: 619 1196
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 486 1815
Size: 486 1329
AfeI AGC'GCT
Cuts at: 0 1512 1815
Size: 1512 303
AflIII A'CryG_T
Cuts at: 0 218 1815
Size: 218 1597
AhdI GACnn_n'nnGTC
Cuts at: 0 954 1815
Size: 954 861
AlwNI CAG_nnn'CTG
Cuts at: 0 1242 1746 1815
Size: 1242 504 69
Fragments arranged by size:
1242 504 69
ApaI G_GGCC'C
Cuts at: 0 103 1067 1089 1815
Size: 103 964 22 726
Fragments arranged by size:
964 726 103 22
ApaLI G'TGCA_C
Cuts at: 0 40 1815
Size: 40 1775
AvaI C'yCGr_G
Cuts at: 0 536 1388 1405 1815
Size: 536 852 17 410
Fragments arranged by size:
852 536 410 17
BamHI G'GATC_C
Cuts at: 0 925 1815
Size: 925 890
BanI G'GyrC_C
Cuts at: 0 20 1171 1464 1620 1798 1815
Size: 20 1151 293 156 178 17
Fragments arranged by size:
1151 293 178 156 20 17
BanII G_rGCy'C
Cuts at: 0 103 265 544 1034 1067 1089 1633 1765
Size: 103 162 279 490 33 22 544 132
Cuts at: 1765 1815
Size: 50
Fragments arranged by size:
544 490 279 162 132 103 50 33
22
BbeI G_GCGC'C
Cuts at: 0 24 1624 1815
Size: 24 1600 191
Fragments arranged by size:
1600 191 24
BbsI GAAGACnn'nnnn_
Cuts at: 0 1704 1815
Size: 1704 111
BbvCI CC'TCA_GC
Cuts at: 0 1425 1815
Size: 1425 390
BglI GCCn_nnn'nGGC
Cuts at: 0 515 1062 1604 1815
Size: 515 547 542 211
Fragments arranged by size:
547 542 515 211
BlpI GC'TnA_GC
Cuts at: 0 1666 1764 1815
Size: 1666 98 51
Fragments arranged by size:
1666 98 51
Bme1580I G_kGCm'C
Cuts at: 0 44 103 1067 1089 1815
Size: 44 59 964 22 726
Fragments arranged by size:
964 726 59 44 22
BmrI ACTGGGnnnn_n'
Cuts at: 0 1042 1130 1295 1815
Size: 1042 88 165 520
Fragments arranged by size:
1042 520 165 88
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 616 1168 1187 1252 1815
Size: 487 129 552 19 65 563
Fragments arranged by size:
563 552 487 129 65 19
Bpu10I CC'TnA_GC
Cuts at: 0 1425 1815
Size: 1425 390
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 301 385 427 1815
Size: 238 63 84 42 1388
Fragments arranged by size:
1388 238 84 63 42
BsaI GGTCTCn'nnnn_
Cuts at: 0 962 1144 1210 1283 1510 1815
Size: 962 182 66 73 227 305
Fragments arranged by size:
962 305 227 182 73 66
BsaAI yAC'GTr
Cuts at: 0 219 1815
Size: 219 1596
BsaHI Gr'CG_yC
Cuts at: 0 21 359 616 1621 1815
Size: 21 338 257 1005 194
Fragments arranged by size:
1005 338 257 194 21
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 34 64 517 547 577 673 703 1815
Size: 34 30 453 30 30 96 30 1112
Fragments arranged by size:
1112 453 96 34 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 424 552 555 682 799 802 1011 1014
Size: 424 128 3 127 117 3 209 3
Cuts at: 1014 1815
Size: 801
Fragments arranged by size:
801 424 209 128 127 117 3 3
3
BseYI C'CCAG_C
Cuts at: 0 495 1060 1114 1258 1294 1370 1593 1815
Size: 495 565 54 144 36 76 223 222
Fragments arranged by size:
565 495 223 222 144 76 54 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 746 1815
Size: 746 1069
BsiEI CG_ry'CG
Cuts at: 0 339 1815
Size: 339 1476
BsiHKAI G_wGCw'C
Cuts at: 0 44 1765 1815
Size: 44 1721 50
Fragments arranged by size:
1721 50 44
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1540 1815
Size: 1540 275
Bsp1286I G_dGCh'C
Cuts at: 0 44 103 265 544 1034 1067 1089 1633
Size: 44 59 162 279 490 33 22 544
Cuts at: 1633 1765 1815
Size: 132 50
Fragments arranged by size:
544 490 279 162 132 59 50 44
33 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 257 375 852 1815
Size: 257 118 477 963
Fragments arranged by size:
963 477 257 118
BsrBI CCG'CTC
Cuts at: 0 270 414 533 1665 1815
Size: 270 144 119 1132 150
Fragments arranged by size:
1132 270 150 144 119
BsrDI GCAATG_nn'
Cuts at: 0 93 235 1815
Size: 93 142 1580
Fragments arranged by size:
1580 142 93
BsrFI r'CCGG_y
Cuts at: 0 1579 1587 1795 1815
Size: 1579 8 208 20
Fragments arranged by size:
1579 208 20 8
BsrGI T'GTAC_A
Cuts at: 0 221 1815
Size: 221 1594
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1242 1746 1815
Size: 1242 504 69
Fragments arranged by size:
1242 504 69
BstYI r'GATC_y
Cuts at: 0 925 1002 1815
Size: 925 77 813
Fragments arranged by size:
925 813 77
Bsu36I CC'TnA_GG
Cuts at: 0 969 1815
Size: 969 846
BtgI C'CryG_G
Cuts at: 0 713 734 883 1774 1815
Size: 713 21 149 891 41
Fragments arranged by size:
891 713 149 41 21
BtsI GCAGTG_nn'
Cuts at: 0 492 867 1815
Size: 492 375 948
Fragments arranged by size:
948 492 375
DraIII CAC_nnn'GTG
Cuts at: 0 1168 1815
Size: 1168 647
DrdI GACnn_nn'nnGTC
Cuts at: 0 358 1407 1456 1815
Size: 358 1049 49 359
Fragments arranged by size:
1049 359 358 49
EaeI y'GGCC_r
Cuts at: 0 716 1815
Size: 716 1099
EarI CTCTTCn'nnn_
Cuts at: 0 573 620 767 1815
Size: 573 47 147 1048
Fragments arranged by size:
1048 573 147 47
EcoICRI GAG'CTC
Cuts at: 0 1763 1815
Size: 1763 52
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 486 487 616 1168 1187 1252 1815
Size: 486 1 129 552 19 65 563
Fragments arranged by size:
563 552 486 129 65 19 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 400 598 1038 1815
Size: 400 198 440 777
Fragments arranged by size:
777 440 400 198
EcoO109I rG'GnC_Cy
Cuts at: 0 99 100 689 937 1043 1064 1086 1239
Size: 99 1 589 248 106 21 22 153
Cuts at: 1239 1420 1815
Size: 181 395
Fragments arranged by size:
589 395 248 181 153 106 99 22
21 1
FspI TGC'GCA
Cuts at: 0 1576 1815
Size: 1576 239
FspAI rTGC'GCAy
Cuts at: 0 1576 1815
Size: 1576 239
HaeII r_GCGC'y
Cuts at: 0 24 1514 1594 1624 1649 1815
Size: 24 1490 80 30 25 166
Fragments arranged by size:
1490 166 80 30 25 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 673 705 940 972 1815
Size: 673 32 235 32 843
Fragments arranged by size:
843 673 235 32 32
HincII GTy'rAC
Cuts at: 0 363 1443 1815
Size: 363 1080 372
Fragments arranged by size:
1080 372 363
KasI G'GCGC_C
Cuts at: 0 20 1620 1815
Size: 20 1600 195
Fragments arranged by size:
1600 195 20
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 614 1181 1815
Size: 614 567 634
Fragments arranged by size:
634 614 567
MscI TGG'CCA
Cuts at: 0 718 1815
Size: 718 1097
MslI CAynn'nnrTG
Cuts at: 0 172 1815
Size: 172 1643
MspA1I CmG'CkG
Cuts at: 0 200 495 639 645 750 1681 1746 1776
Size: 200 295 144 6 105 931 65 30
Cuts at: 1776 1815
Size: 39
Fragments arranged by size:
931 295 200 144 105 65 39 30
6
NaeI GCC'GGC
Cuts at: 0 1589 1815
Size: 1589 226
NarI GG'CG_CC
Cuts at: 0 21 1621 1815
Size: 21 1600 194
Fragments arranged by size:
1600 194 21
NcoI C'CATG_G
Cuts at: 0 713 883 1815
Size: 713 170 932
Fragments arranged by size:
932 713 170
NgoMIV G'CCGG_C
Cuts at: 0 1587 1815
Size: 1587 228
NspI r_CATG'y
Cuts at: 0 177 1815
Size: 177 1638
PasI CC'CwG_GG
Cuts at: 0 95 998 1034 1082 1626 1815
Size: 95 903 36 48 544 189
Fragments arranged by size:
903 544 189 95 48 36
PfoI T'CCnGG_A
Cuts at: 0 818 1815
Size: 818 997
PmlI CAC'GTG
Cuts at: 0 219 1815
Size: 219 1596
PpuMI rG'GwC_Cy
Cuts at: 0 689 1043 1420 1815
Size: 689 354 377 395
Fragments arranged by size:
689 395 377 354
PspOMI G'GGCC_C
Cuts at: 0 99 1063 1085 1815
Size: 99 964 22 730
Fragments arranged by size:
964 730 99 22
PstI C_TGCA'G
Cuts at: 0 638 644 948 1815
Size: 638 6 304 867
Fragments arranged by size:
867 638 304 6
PvuII CAG'CTG
Cuts at: 0 495 639 645 1681 1815
Size: 495 144 6 1036 134
Fragments arranged by size:
1036 495 144 134 6
SacI G_AGCT'C
Cuts at: 0 1765 1815
Size: 1765 50
SacII CC_GC'GG
Cuts at: 0 1777 1815
Size: 1777 38
SanDI GG'GwC_CC
Cuts at: 0 1420 1815
Size: 1420 395
SexAI A'CCwGG_T
Cuts at: 0 1164 1815
Size: 1164 651
SfcI C'TryA_G
Cuts at: 0 525 634 640 944 1641 1815
Size: 525 109 6 304 697 174
Fragments arranged by size:
697 525 304 174 109 6
SfoI GGC'GCC
Cuts at: 0 22 1622 1815
Size: 22 1600 193
Fragments arranged by size:
1600 193 22
SgrAI Cr'CCGG_yG
Cuts at: 0 1587 1815
Size: 1587 228
SmaI CCC'GGG
Cuts at: 0 538 1815
Size: 538 1277
SmlI C'TyrA_G
Cuts at: 0 253 316 400 442 1815
Size: 253 63 84 42 1373
Fragments arranged by size:
1373 253 84 63 42
SphI G_CATG'C
Cuts at: 0 177 1815
Size: 177 1638
StyI C'CwwG_G
Cuts at: 0 449 713 883 1132 1220 1436 1815
Size: 449 264 170 249 88 216 379
Fragments arranged by size:
449 379 264 249 216 170 88
TatI w'GTAC_w
Cuts at: 0 221 329 1815
Size: 221 108 1486
Fragments arranged by size:
1486 221 108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 34 66 1815
Size: 34 32 1749
Fragments arranged by size:
1749 34 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 456 822 1227 1266 1815
Size: 456 366 405 39 549
Fragments arranged by size:
549 456 405 366 39
XmaI C'CCGG_G
Cuts at: 0 536 1815
Size: 536 1279
XmnI GAAnn'nnTTC
Cuts at: 0 1492 1815
Size: 1492 323
ZraI GAC'GTC
Cuts at: 0 617 1815
Size: 617 1198
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AhdI AlwNI ApaI
ApaLI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BglI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BstAPI
BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I FspI FspAI HaeII Hin4I
HincII KasI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PfoI PmlI PpuMI PspOMI
PstI PvuII SacI SacII SanDI SexAI SfcI SfoI
SgrAI SmaI SmlI SphI StyI TatI TstI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI AleI AlfI AloI
ApoI AscI AseI AsiSI AvrII BaeI BcgI BciVI
BclI BfrBI BglII BmgBI BmtI BplI BsaBI BsaWI
BsiWI BsmI BspEI BspHI BssHII BssSI BstBI BstEII
BstXI BstZ17I BtgZI ClaI CspCI DraI EagI EciI
EcoRI EcoRV FalI FseI HindIII HpaI KpnI MfeI
MluI NdeI NheI NotI NruI NsiI PacI PciI
PflMI PmeI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SalI SapI SbfI ScaI SfiI SgfI SnaBI
SpeI SrfI SspI StuI SwaI TaqII Tth111I XbaI
XhoI