(Circular) (Six-base) MAPSORT of: pFN21AB0011.seq Check: 1487 from: 1 to: 6145 pFN21AB0011 6145 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3955 278 Size: 53 83 186 936 2419 2468 Fragments arranged by size: 2468 2419 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3488 1937 Size: 1551 4594 Acc65I G'GTAC_C Cuts at: 3466 3466 Size: 6145 AclI AA'CG_TT Cuts at: 1563 4272 4645 1563 Size: 2709 373 3063 Fragments arranged by size: 3063 2709 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3190 4208 5592 5860 1632 Size: 1558 1018 1384 268 1917 Fragments arranged by size: 1917 1558 1384 1018 268 AflII C'TTAA_G Cuts at: 820 1017 3227 820 Size: 197 2210 3738 Fragments arranged by size: 3738 2210 197 AflIII A'CryG_T Cuts at: 1203 5044 6021 1203 Size: 3841 977 1327 Fragments arranged by size: 3841 1327 977 AgeI A'CCGG_T Cuts at: 4976 4976 Size: 6145 AhdI GACnn_n'nnGTC Cuts at: 2054 4874 2054 Size: 2820 3325 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6145 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6145 AlwNI CAG_nnn'CTG Cuts at: 2165 2299 2318 3888 5460 2165 Size: 134 19 1570 1572 2850 Fragments arranged by size: 2850 1572 1570 134 19 ApaI G_GGCC'C Cuts at: 2467 2588 2604 2652 2850 2467 Size: 121 16 48 198 5762 Fragments arranged by size: 5762 198 121 48 16 ApaLI G'TGCA_C Cuts at: 4202 5358 4202 Size: 1156 4989 ApoI r'AATT_y Cuts at: 1493 3454 3680 3832 5941 1493 Size: 1961 226 152 2109 1697 Fragments arranged by size: 2109 1961 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4699 160 Size: 872 3667 1606 Fragments arranged by size: 3667 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6145 AvaI C'yCGr_G Cuts at: 1870 1943 2026 2035 2059 2080 2307 2418 2637 Size: 73 83 9 24 21 227 111 219 Cuts at: 2637 2706 2882 3470 1870 Size: 69 176 588 4545 Fragments arranged by size: 4545 588 227 219 176 111 83 73 69 24 21 9 AvrII C'CTAG_G Cuts at: 2719 2719 Size: 6145 BamHI G'GATC_C Cuts at: 3475 3842 3475 Size: 367 5778 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2191 2819 3466 4921 618 Size: 325 217 627 404 628 647 1455 1842 Fragments arranged by size: 1842 1455 647 628 627 404 325 217 BanII G_rGCy'C Cuts at: 1391 2158 2467 2588 2604 2644 2652 2663 2850 Size: 767 309 121 16 40 8 11 187 Cuts at: 2850 2895 3033 3350 3464 1391 Size: 45 138 317 114 4072 Fragments arranged by size: 4072 767 317 309 187 138 121 114 45 40 16 11 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3048 928 Size: 971 1149 4025 Fragments arranged by size: 4025 1149 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4336 4370 1903 Size: 34 2399 34 3678 Fragments arranged by size: 3678 2399 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4037 5247 4037 Size: 1210 4935 BclI T'GATC_A Cuts at: 1548 1548 Size: 6145 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2373 2810 2986 Size: 107 122 71 631 605 701 437 176 Cuts at: 2986 4756 136 Size: 1770 1525 Fragments arranged by size: 1770 1525 701 631 605 437 176 122 107 71 BglII A'GATC_T Cuts at: 6140 6140 Size: 6145 BlpI GC'TnA_GC Cuts at: 3562 3562 Size: 6145 Bme1580I G_kGCm'C Cuts at: 1790 2196 2467 2588 2604 2652 2824 2850 4206 Size: 406 271 121 16 48 172 26 1356 Cuts at: 4206 5362 1790 Size: 1156 2573 Fragments arranged by size: 2573 1356 1156 406 271 172 121 48 26 16 BmgBI CAC'GTC Cuts at: 1318 3400 1318 Size: 2082 4063 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3876 4834 442 Size: 416 53 83 399 24 2459 958 1753 Fragments arranged by size: 2459 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2793 1056 Size: 1737 4408 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2700 2700 Size: 6145 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2142 2321 2628 2801 3146 4805 1239 Size: 903 179 307 173 345 1659 2579 Fragments arranged by size: 2579 1659 903 345 307 179 173 Bpu10I CC'TnA_GC Cuts at: 2889 2889 Size: 6145 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3038 4270 5135 5433 3038 Size: 1232 865 298 3750 Fragments arranged by size: 3750 1232 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4808 882 Size: 617 3309 2219 Fragments arranged by size: 3309 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2497 3310 3936 493 Size: 711 1293 813 626 2702 Fragments arranged by size: 2702 1293 813 711 626 BsaBI GATnn'nnATC Cuts at: 6139 6139 Size: 6145 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3952 4334 4969 275 Size: 53 83 186 936 2419 382 635 1451 Fragments arranged by size: 2419 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3147 3387 4578 4976 5250 5397 5808 3147 Size: 240 1191 398 274 147 411 3484 Fragments arranged by size: 3484 1191 411 398 274 240 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2037 2067 1178 Size: 30 165 30 634 30 5256 Fragments arranged by size: 5256 634 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2515 2798 1186 Size: 1329 283 4533 Fragments arranged by size: 4533 1329 283 BseYI C'CCAG_C Cuts at: 2009 2236 2656 2713 2774 2824 2849 3105 3116 Size: 227 420 57 61 50 25 256 11 Cuts at: 3116 3299 3433 5348 5844 2009 Size: 183 134 1915 496 2310 Fragments arranged by size: 2310 1915 496 420 256 227 183 134 61 57 50 25 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2061 2655 1163 Size: 573 325 594 4653 Fragments arranged by size: 4653 594 573 325 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3595 4356 5384 1540 Size: 275 186 1594 761 1028 2301 Fragments arranged by size: 2301 1594 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4206 4291 5362 4206 Size: 85 1071 4989 Fragments arranged by size: 4989 1071 85 BsmI GAATG_Cn' Cuts at: 2943 3081 3656 3749 2943 Size: 138 575 93 5339 Fragments arranged by size: 5339 575 138 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2158 2196 2467 2588 2604 2644 2652 Size: 399 368 38 271 121 16 40 8 Cuts at: 2652 2663 2824 2850 2895 3033 3350 3464 4206 Size: 11 161 26 45 138 317 114 742 Cuts at: 4206 4291 5362 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 368 317 271 161 138 121 114 85 45 40 38 26 16 11 8 BspEI T'CCGG_A Cuts at: 3147 5808 3147 Size: 2661 3484 BspHI T'CATG_A Cuts at: 4034 5764 4034 Size: 1730 4415 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2221 2275 3500 844 Size: 1377 54 1225 3489 Fragments arranged by size: 3489 1377 1225 54 BsrBI CCG'CTC Cuts at: 1394 1823 2519 4032 1394 Size: 429 696 1513 3507 Fragments arranged by size: 3507 1513 696 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2876 3178 4640 4814 65 Size: 1183 1628 302 1462 174 1396 Fragments arranged by size: 1628 1462 1396 1183 302 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2537 4789 4976 1700 Size: 110 727 2252 187 2869 Fragments arranged by size: 2869 2252 727 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6145 BssHII G'CGCG_C Cuts at: 1925 3313 1925 Size: 1388 4757 BssSI C'ACGA_G Cuts at: 2579 4205 5217 2579 Size: 1626 1012 3507 Fragments arranged by size: 3507 1626 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2318 2819 794 Size: 1524 501 4120 Fragments arranged by size: 4120 1524 501 BstBI TT'CG_AA Cuts at: 4949 4949 Size: 6145 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6145 BstXI CCAn_nnnn'nTGG Cuts at: 3124 3124 Size: 6145 BstYI r'GATC_y Cuts at: 1972 3150 3163 3475 3842 4227 4244 5696 5782 Size: 1178 13 312 367 385 17 1452 86 Cuts at: 5782 5794 6140 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1178 385 367 346 312 86 17 13 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2052 2205 2340 2664 2805 2920 Size: 552 939 48 153 135 324 141 115 Cuts at: 2920 513 Size: 3738 Fragments arranged by size: 3738 939 552 324 153 141 135 115 48 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2240 487 Size: 683 56 1014 4392 Fragments arranged by size: 4392 1014 683 56 BtsI GCAGTG_nn' Cuts at: 2763 3661 4455 4475 2763 Size: 898 794 20 4433 Fragments arranged by size: 4433 898 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6145 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6110 DraI TTT'AAA Cuts at: 3449 3449 Size: 6145 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5152 809 Size: 507 3836 1802 Fragments arranged by size: 3836 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2802 2868 2987 Size: 54 1425 50 275 18 972 66 119 Cuts at: 2987 3592 4481 6110 8 Size: 605 889 1629 43 Fragments arranged by size: 1629 1425 972 889 605 275 119 66 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3592 1537 Size: 275 1780 4090 Fragments arranged by size: 4090 1780 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2685 2745 4075 1353 Size: 1332 60 1330 3423 Fragments arranged by size: 3423 1332 1330 60 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2135 2243 4732 5106 5252 387 Size: 1064 138 546 108 2489 374 146 1280 Fragments arranged by size: 2489 1280 1064 546 374 146 138 108 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2142 2321 2628 2801 3146 3190 4208 Size: 393 510 179 307 173 345 44 1018 Cuts at: 4208 4805 5592 5860 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1018 787 597 510 393 345 307 268 179 173 44 EcoNI CCTnn'n_nnAGG Cuts at: 2361 2361 Size: 6145 EcoO109I rG'GnC_Cy Cuts at: 2048 2063 2233 2366 2463 2464 2575 2585 2601 Size: 15 170 133 97 1 111 10 16 Cuts at: 2601 2648 2649 2679 3022 3112 3295 2048 Size: 47 1 30 343 90 183 4898 Fragments arranged by size: 4898 343 183 170 133 111 97 90 47 30 16 15 10 1 1 EcoRI G'AATT_C Cuts at: 3454 3832 3454 Size: 378 5767 FspI TGC'GCA Cuts at: 2942 4651 2942 Size: 1709 4436 HaeII r_GCGC'y Cuts at: 1678 2073 5292 1678 Size: 395 3219 2531 Fragments arranged by size: 3219 2531 395 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2040 2072 4786 4818 4860 Size: 32 135 32 468 32 2714 32 42 Cuts at: 4860 4892 1373 Size: 32 2626 Fragments arranged by size: 2714 2626 468 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3489 3735 669 Size: 988 281 1551 246 3079 Fragments arranged by size: 3079 1551 988 281 246 HindIII A'AGCT_T Cuts at: 748 3362 748 Size: 2614 3531 HpaI GTT'AAC Cuts at: 3735 3735 Size: 6145 KpnI G_GTAC'C Cuts at: 3470 3470 Size: 6145 MfeI C'AATT_G Cuts at: 3744 3744 Size: 6145 MluI A'CGCG_T Cuts at: 6021 6021 Size: 6145 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2889 5259 5443 1305 Size: 1584 2370 184 2007 Fragments arranged by size: 2370 2007 1584 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2870 10 Size: 54 1425 343 1038 3285 Fragments arranged by size: 3285 1425 1038 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4103 4462 4621 518 Size: 648 2937 359 159 2042 Fragments arranged by size: 2937 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2240 2315 2447 2656 2828 2860 2922 2938 Size: 634 75 132 209 172 32 62 16 Cuts at: 2938 3092 3105 3561 4238 5386 5631 1606 Size: 154 13 456 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 634 456 245 209 172 154 132 75 62 32 16 13 NaeI GCC'GGC Cuts at: 2539 2539 Size: 6145 NcoI C'CATG_G Cuts at: 513 1065 2004 2340 2664 513 Size: 552 939 336 324 3994 Fragments arranged by size: 3994 939 552 336 324 NdeI CA'TA_TG Cuts at: 387 387 Size: 6145 NgoMIV G'CCGG_C Cuts at: 2537 2537 Size: 6145 NheI G'CTAG_C Cuts at: 1052 2789 1052 Size: 1737 4408 NotI GC'GGCC_GC Cuts at: 3592 3592 Size: 6145 NruI TCG'CGA Cuts at: 2267 2267 Size: 6145 NspI r_CATG'y Cuts at: 1132 1229 3379 3503 5048 1132 Size: 97 2150 124 1545 2229 Fragments arranged by size: 2229 2150 1545 124 97 PasI CC'CwG_GG Cuts at: 1348 3034 1348 Size: 1686 4459 PciI A'CATG_T Cuts at: 5044 5044 Size: 6145 PflMI CCAn_nnn'nTGG Cuts at: 1108 2165 1108 Size: 1057 5088 PmeI GTTT'AAAC Cuts at: 3449 3449 Size: 6145 PmlI CAC'GTG Cuts at: 2497 2497 Size: 6145 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4214 4246 5727 5759 4214 Size: 32 1481 32 4600 Fragments arranged by size: 4600 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2048 2063 2233 3022 3112 3295 2048 Size: 15 170 789 90 183 4898 Fragments arranged by size: 4898 789 183 170 90 15 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6145 PsiI TTA'TAA Cuts at: 3715 3715 Size: 6145 PspOMI G'GGCC_C Cuts at: 2463 2584 2600 2648 2846 2463 Size: 121 16 48 198 5762 Fragments arranged by size: 5762 198 121 48 16 PstI C_TGCA'G Cuts at: 830 2323 2533 2839 3497 830 Size: 1493 210 306 658 3478 Fragments arranged by size: 3478 1493 658 306 210 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6145 PvuII CAG'CTG Cuts at: 2315 2447 2828 3105 2315 Size: 132 381 277 5355 Fragments arranged by size: 5355 381 277 132 SacII CC_GC'GG Cuts at: 2923 2923 Size: 6145 SalI G'TCGA_C Cuts at: 1936 3487 1936 Size: 1551 4594 SapI GCTCTTCn'nnn_ Cuts at: 2685 2685 Size: 6145 SbfI CC_TGCA'GG Cuts at: 3497 3497 Size: 6145 ScaI AGT'ACT Cuts at: 4393 6105 4393 Size: 1712 4433 SfcI C'TryA_G Cuts at: 826 1045 2319 2501 2529 2835 3493 4628 5309 Size: 219 1274 182 28 306 658 1135 681 Cuts at: 5309 5500 826 Size: 191 1471 Fragments arranged by size: 1471 1274 1135 681 658 306 219 191 182 28 SfiI GGCCn_nnn'nGGCC Cuts at: 2986 2986 Size: 6145 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6145 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6145 SmaI CCC'GGG Cuts at: 1872 2028 2037 2061 2082 3472 1872 Size: 156 9 24 21 1390 4545 Fragments arranged by size: 4545 1390 156 24 21 9 SmlI C'TyrA_G Cuts at: 820 1017 1943 2418 3017 3227 4249 5150 5412 Size: 197 926 475 599 210 1022 901 262 Cuts at: 5412 820 Size: 1553 Fragments arranged by size: 1553 1022 926 901 599 475 262 210 197 SnaBI TAC'GTA Cuts at: 493 3936 493 Size: 3443 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6145 SphI G_CATG'C Cuts at: 1132 3503 1132 Size: 2371 3774 SspI AAT'ATT Cuts at: 5 52 4069 5 Size: 47 4017 2081 Fragments arranged by size: 4017 2081 47 StuI AGG'CCT Cuts at: 1517 2724 3006 1517 Size: 1207 282 4656 Fragments arranged by size: 4656 1207 282 StyI C'CwwG_G Cuts at: 513 1065 2004 2340 2664 2719 3234 3267 3584 Size: 552 939 336 324 55 515 33 317 Cuts at: 3584 513 Size: 3074 Fragments arranged by size: 3074 939 552 515 336 324 317 55 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2336 2629 3026 4172 4189 4342 4527 Size: 1255 756 293 397 1146 17 153 185 Cuts at: 4527 4972 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1146 756 445 397 293 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4391 6103 96 Size: 274 80 33 51 1443 2414 1712 138 Fragments arranged by size: 2414 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2037 2069 2317 2349 3142 3174 6105 6137 2037 Size: 32 248 32 793 32 2931 32 2045 Fragments arranged by size: 2931 2045 793 248 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3401 1316 Size: 2085 4060 XbaI T'CTAG_A Cuts at: 3481 3481 Size: 6145 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2857 2857 Size: 6145 XhoI C'TCGA_G Cuts at: 1943 2418 1943 Size: 475 5670 XmaI C'CCGG_G Cuts at: 1870 2026 2035 2059 2080 3470 1870 Size: 156 9 24 21 1390 4545 Fragments arranged by size: 4545 1390 156 24 21 9 XmnI GAAnn'nnTTC Cuts at: 4274 4274 Size: 6145 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3953 276 Size: 53 83 186 936 2419 2468 Fragments arranged by size: 2468 2419 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NsiI PacI PfoI PspXI PsrI RsrII SacI SanDI SexAI SfoI SrfI SwaI