Restriction map for
pFN21AB0011
(Linear) (Six-base) MAPSORT of: KIBB0011.seq Check: 500 from: 1 to: 1440
KIBB0011.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1185 1440
Size: 1185 255
AflII C'TTAA_G
Cuts at: 0 1222 1440
Size: 1222 218
AhdI GACnn_n'nnGTC
Cuts at: 0 49 1440
Size: 49 1391
AlwNI CAG_nnn'CTG
Cuts at: 0 160 294 313 1440
Size: 160 134 19 1127
Fragments arranged by size:
1127 160 134 19
ApaI G_GGCC'C
Cuts at: 0 462 583 599 647 845 1440
Size: 462 121 16 48 198 595
Fragments arranged by size:
595 462 198 121 48 16
AvaI C'yCGr_G
Cuts at: 0 21 30 54 75 302 413 632 701
Size: 21 9 24 21 227 111 219 69
Cuts at: 701 877 1440
Size: 176 563
Fragments arranged by size:
563 227 219 176 111 69 24 21
21 9
AvrII C'CTAG_G
Cuts at: 0 714 1440
Size: 714 726
BanI G'GyrC_C
Cuts at: 0 186 814 1440
Size: 186 628 626
Fragments arranged by size:
628 626 186
BanII G_rGCy'C
Cuts at: 0 153 462 583 599 639 647 658 845
Size: 153 309 121 16 40 8 11 187
Cuts at: 845 890 1028 1345 1440
Size: 45 138 317 95
Fragments arranged by size:
317 309 187 153 138 121 95 45
40 16 11 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 1043 1440
Size: 1043 397
BglI GCCn_nnn'nGGC
Cuts at: 0 368 805 981 1440
Size: 368 437 176 459
Fragments arranged by size:
459 437 368 176
Bme1580I G_kGCm'C
Cuts at: 0 191 462 583 599 647 819 845 1440
Size: 191 271 121 16 48 172 26 595
Fragments arranged by size:
595 271 191 172 121 48 26 16
BmgBI CAC'GTC
Cuts at: 0 1395 1440
Size: 1395 45
BmtI G_CTAG'C
Cuts at: 0 788 1440
Size: 788 652
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 695 1440
Size: 695 745
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 316 623 796 1141 1440
Size: 137 179 307 173 345 299
Fragments arranged by size:
345 307 299 179 173 137
Bpu10I CC'TnA_GC
Cuts at: 0 884 1440
Size: 884 556
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1033 1440
Size: 1033 407
BsaAI yAC'GTr
Cuts at: 0 492 1305 1440
Size: 492 813 135
Fragments arranged by size:
813 492 135
BsaWI w'CCGG_w
Cuts at: 0 1142 1382 1440
Size: 1142 240 58
Fragments arranged by size:
1142 240 58
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 32 62 1440
Size: 32 30 1378
Fragments arranged by size:
1378 32 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 510 793 1440
Size: 510 283 647
Fragments arranged by size:
647 510 283
BseYI C'CCAG_C
Cuts at: 0 4 231 651 708 769 819 844 1100
Size: 4 227 420 57 61 50 25 256
Cuts at: 1100 1111 1294 1428 1440
Size: 11 183 134 12
Fragments arranged by size:
420 256 227 183 134 61 57 50
25 12 11 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 650 1440
Size: 56 594 790
Fragments arranged by size:
790 594 56
BsmI GAATG_Cn'
Cuts at: 0 938 1076 1440
Size: 938 138 364
Fragments arranged by size:
938 364 138
Bsp1286I G_dGCh'C
Cuts at: 0 153 191 462 583 599 639 647 658
Size: 153 38 271 121 16 40 8 11
Cuts at: 658 819 845 890 1028 1345 1440
Size: 161 26 45 138 317 95
Fragments arranged by size:
317 271 161 153 138 121 95 45
40 38 26 16 11 8
BspEI T'CCGG_A
Cuts at: 0 1142 1440
Size: 1142 298
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 216 270 1440
Size: 216 54 1170
Fragments arranged by size:
1170 216 54
BsrBI CCG'CTC
Cuts at: 0 514 1440
Size: 514 926
BsrDI GCAATG_nn'
Cuts at: 0 871 1173 1440
Size: 871 302 267
Fragments arranged by size:
871 302 267
BsrFI r'CCGG_y
Cuts at: 0 532 1440
Size: 532 908
BssHII G'CGCG_C
Cuts at: 0 1308 1440
Size: 1308 132
BssSI C'ACGA_G
Cuts at: 0 574 1440
Size: 574 866
BstAPI GCAn_nnn'nTGC
Cuts at: 0 313 814 1440
Size: 313 501 626
Fragments arranged by size:
626 501 313
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1119 1440
Size: 1119 321
BstYI r'GATC_y
Cuts at: 0 1145 1158 1440
Size: 1145 13 282
Fragments arranged by size:
1145 282 13
BtgI C'CryG_G
Cuts at: 0 47 200 335 659 800 915 1440
Size: 47 153 135 324 141 115 525
Fragments arranged by size:
525 324 153 141 135 115 47
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 235 1440
Size: 235 1205
BtsI GCAGTG_nn'
Cuts at: 0 758 1440
Size: 758 682
EaeI y'GGCC_r
Cuts at: 0 797 863 982 1440
Size: 797 66 119 458
Fragments arranged by size:
797 458 119 66
EarI CTCTTCn'nnn_
Cuts at: 0 680 740 1440
Size: 680 60 700
Fragments arranged by size:
700 680 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 130 238 1440
Size: 130 108 1202
Fragments arranged by size:
1202 130 108
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 316 623 796 1141 1185 1440
Size: 137 179 307 173 345 44 255
Fragments arranged by size:
345 307 255 179 173 137 44
EcoNI CCTnn'n_nnAGG
Cuts at: 0 356 1440
Size: 356 1084
EcoO109I rG'GnC_Cy
Cuts at: 0 43 58 228 361 458 459 570 580
Size: 43 15 170 133 97 1 111 10
Cuts at: 580 596 643 644 674 1017 1107 1290 1440
Size: 16 47 1 30 343 90 183 150
Fragments arranged by size:
343 183 170 150 133 111 97 90
47 43 30 16 15 10 1 1
FspI TGC'GCA
Cuts at: 0 937 1440
Size: 937 503
HaeII r_GCGC'y
Cuts at: 0 68 1440
Size: 68 1372
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 35 67 1440
Size: 35 32 1373
Fragments arranged by size:
1373 35 32
HindIII A'AGCT_T
Cuts at: 0 1357 1440
Size: 1357 83
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 884 1440
Size: 884 556
MscI TGG'CCA
Cuts at: 0 865 1440
Size: 865 575
MspA1I CmG'CkG
Cuts at: 0 235 310 442 651 823 855 917 933
Size: 235 75 132 209 172 32 62 16
Cuts at: 933 1087 1100 1440
Size: 154 13 340
Fragments arranged by size:
340 235 209 172 154 132 75 62
32 16 13
NaeI GCC'GGC
Cuts at: 0 534 1440
Size: 534 906
NcoI C'CATG_G
Cuts at: 0 335 659 1440
Size: 335 324 781
Fragments arranged by size:
781 335 324
NgoMIV G'CCGG_C
Cuts at: 0 532 1440
Size: 532 908
NheI G'CTAG_C
Cuts at: 0 784 1440
Size: 784 656
NruI TCG'CGA
Cuts at: 0 262 1440
Size: 262 1178
NspI r_CATG'y
Cuts at: 0 1374 1440
Size: 1374 66
PasI CC'CwG_GG
Cuts at: 0 1029 1440
Size: 1029 411
PflMI CCAn_nnn'nTGG
Cuts at: 0 160 1440
Size: 160 1280
PmlI CAC'GTG
Cuts at: 0 492 1440
Size: 492 948
PpuMI rG'GwC_Cy
Cuts at: 0 43 58 228 1017 1107 1290 1440
Size: 43 15 170 789 90 183 150
Fragments arranged by size:
789 183 170 150 90 43 15
PspOMI G'GGCC_C
Cuts at: 0 458 579 595 643 841 1440
Size: 458 121 16 48 198 599
Fragments arranged by size:
599 458 198 121 48 16
PstI C_TGCA'G
Cuts at: 0 318 528 834 1440
Size: 318 210 306 606
Fragments arranged by size:
606 318 306 210
PvuII CAG'CTG
Cuts at: 0 310 442 823 1100 1440
Size: 310 132 381 277 340
Fragments arranged by size:
381 340 310 277 132
SacII CC_GC'GG
Cuts at: 0 918 1440
Size: 918 522
SapI GCTCTTCn'nnn_
Cuts at: 0 680 1440
Size: 680 760
SfcI C'TryA_G
Cuts at: 0 314 496 524 830 1440
Size: 314 182 28 306 610
Fragments arranged by size:
610 314 306 182 28
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 981 1440
Size: 981 459
SmaI CCC'GGG
Cuts at: 0 23 32 56 77 1440
Size: 23 9 24 21 1363
Fragments arranged by size:
1363 24 23 21 9
SmlI C'TyrA_G
Cuts at: 0 413 1012 1222 1440
Size: 413 599 210 218
Fragments arranged by size:
599 413 218 210
StuI AGG'CCT
Cuts at: 0 719 1001 1440
Size: 719 282 439
Fragments arranged by size:
719 439 282
StyI C'CwwG_G
Cuts at: 0 335 659 714 1229 1262 1440
Size: 335 324 55 515 33 178
Fragments arranged by size:
515 335 324 178 55 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 331 624 1021 1440
Size: 331 293 397 419
Fragments arranged by size:
419 397 331 293
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 32 64 312 344 1137 1169 1440
Size: 32 32 248 32 793 32 271
Fragments arranged by size:
793 271 248 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1396 1440
Size: 1396 44
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 852 1440
Size: 852 588
XhoI C'TCGA_G
Cuts at: 0 413 1440
Size: 413 1027
XmaI C'CCGG_G
Cuts at: 0 21 30 54 75 1440
Size: 21 9 24 21 1365
Fragments arranged by size:
1365 24 21 21 9
Enzymes that do cut:
AcuI AflII AhdI AlwNI ApaI AvaI AvrII BanI
BanII BbsI BglI Bme1580I BmgBI BmtI BplI BpmI
Bpu10I BpuEI BsaAI BsaWI BsaXI BseRI BseYI BsgI
BsmI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI EaeI
EarI EciI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I
HindIII MmeI MscI MspA1I NaeI NcoI NgoMIV NheI
NruI NspI PasI PflMI PmlI PpuMI PspOMI PstI
PvuII SacII SapI SfcI SfiI SmaI SmlI StuI
StyI TaqII TstI Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI BmrI BsaI BsaBI BsaHI BsiEI BsiHKAI
BsiWI BsmBI BspHI BsrGI BstBI BstEII BstZ17I Bsu36I
ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoRI
EcoRV FalI FseI FspAI HincII HpaI KasI KpnI
MfeI MluI MslI NarI NdeI NotI NsiI PacI
PciI PfoI PmeI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SalI SanDI SbfI ScaI SexAI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI TatI XbaI XmnI ZraI