(Circular) (Six-base) MAPSORT of: pFN21AA1503.seq Check: 9012 from: 1 to: 7075 pFN21AA1503 7075 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4885 278 Size: 53 83 186 936 3349 2468 Fragments arranged by size: 3349 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2421 4418 1937 Size: 484 1997 4594 Fragments arranged by size: 4594 1997 484 Acc65I G'GTAC_C Cuts at: 4396 4396 Size: 7075 AclI AA'CG_TT Cuts at: 1563 5202 5575 1563 Size: 3639 373 3063 Fragments arranged by size: 3639 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2396 3050 5138 6522 6790 1632 Size: 764 654 2088 1384 268 1917 Fragments arranged by size: 2088 1917 1384 764 654 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6878 AflIII A'CryG_T Cuts at: 1203 5974 6951 1203 Size: 4771 977 1327 Fragments arranged by size: 4771 1327 977 AgeI A'CCGG_T Cuts at: 5906 5906 Size: 7075 AhdI GACnn_n'nnGTC Cuts at: 3816 5804 3816 Size: 1988 5087 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7075 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2130 1184 Size: 946 6129 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2825 2857 2825 Size: 32 7043 AlwNI CAG_nnn'CTG Cuts at: 3424 4265 4818 6390 3424 Size: 841 553 1572 4109 Fragments arranged by size: 4109 1572 841 553 ApaI G_GGCC'C Cuts at: 2501 2501 Size: 7075 ApaLI G'TGCA_C Cuts at: 3656 3746 5132 6288 3656 Size: 90 1386 1156 4443 Fragments arranged by size: 4443 1386 1156 90 ApoI r'AATT_y Cuts at: 1493 2815 3965 4384 4610 4762 6871 1493 Size: 1322 1150 419 226 152 2109 1697 Fragments arranged by size: 2109 1697 1322 1150 419 226 152 AseI AT'TA_AT Cuts at: 160 1032 5629 160 Size: 872 4597 1606 Fragments arranged by size: 4597 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7075 AvaI C'yCGr_G Cuts at: 1870 1943 2082 2219 2250 3144 4355 4400 1870 Size: 73 139 137 31 894 1211 45 4545 Fragments arranged by size: 4545 1211 894 139 137 73 45 31 BamHI G'GATC_C Cuts at: 4405 4772 4405 Size: 367 6708 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2950 3245 4396 5851 618 Size: 325 217 627 1163 295 1151 1455 1842 Fragments arranged by size: 1842 1455 1163 1151 627 325 295 217 BanII G_rGCy'C Cuts at: 1391 2154 2220 2501 3621 4221 4356 4394 1391 Size: 763 66 281 1120 600 135 38 4072 Fragments arranged by size: 4072 1120 763 600 281 135 66 38 BbeI G_GCGC'C Cuts at: 2954 2954 Size: 7075 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3375 928 Size: 971 1476 4628 Fragments arranged by size: 4628 1476 971 BbvCI CC'TCA_GC Cuts at: 3433 3433 Size: 7075 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5266 5300 1903 Size: 34 3329 34 3678 Fragments arranged by size: 3678 3329 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3347 3841 4967 6177 3347 Size: 494 1126 1210 4245 Fragments arranged by size: 4245 1210 1126 494 BclI T'GATC_A Cuts at: 1548 3096 3426 1548 Size: 1548 330 5197 Fragments arranged by size: 5197 1548 330 BfrBI ATG'CAT Cuts at: 2859 2859 Size: 7075 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2084 4360 5686 Size: 107 122 71 631 605 412 2276 1326 Cuts at: 5686 136 Size: 1525 Fragments arranged by size: 2276 1525 1326 631 605 412 122 107 71 BglII A'GATC_T Cuts at: 2550 7070 2550 Size: 4520 2555 BlpI GC'TnA_GC Cuts at: 2148 4492 2148 Size: 2344 4731 Bme1580I G_kGCm'C Cuts at: 1790 2501 2658 2791 3250 3660 3750 5136 6292 Size: 711 157 133 459 410 90 1386 1156 Cuts at: 6292 1790 Size: 2573 Fragments arranged by size: 2573 1386 1156 711 459 410 157 133 90 BmgBI CAC'GTC Cuts at: 1318 3457 1318 Size: 2139 4936 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3625 4253 4806 Size: 416 53 83 399 24 2208 628 553 Cuts at: 4806 5764 442 Size: 958 1753 Fragments arranged by size: 2208 1753 958 628 553 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7075 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2669 2849 3299 3425 3635 5735 1239 Size: 1430 180 450 126 210 2100 2579 Fragments arranged by size: 2579 2100 1430 450 210 180 126 Bpu10I CC'TnA_GC Cuts at: 2169 3090 3433 4350 2169 Size: 921 343 917 4894 Fragments arranged by size: 4894 921 917 343 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2175 4235 5200 6065 6363 2175 Size: 2060 965 865 298 2887 Fragments arranged by size: 2887 2060 965 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2735 4318 5738 882 Size: 617 1236 1583 1420 2219 Fragments arranged by size: 2219 1583 1420 1236 617 BsaAI yAC'GTr Cuts at: 493 1204 3625 4866 493 Size: 711 2421 1241 2702 Fragments arranged by size: 2702 2421 1241 711 BsaBI GATnn'nnATC Cuts at: 7069 7069 Size: 7075 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2951 3670 4882 5264 Size: 53 83 186 936 1418 719 1212 382 Cuts at: 5264 5899 275 Size: 635 1451 Fragments arranged by size: 1451 1418 1212 936 719 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3638 5508 5906 6180 6327 6738 3638 Size: 1870 398 274 147 411 3975 Fragments arranged by size: 3975 1870 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2825 2855 1178 Size: 30 165 30 1422 30 5398 Fragments arranged by size: 5398 1422 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2177 2846 3978 1186 Size: 991 669 1132 4283 Fragments arranged by size: 4283 1132 991 669 BseYI C'CCAG_C Cuts at: 2386 3204 3651 4180 4196 4287 6278 6774 2386 Size: 818 447 529 16 91 1991 496 2687 Fragments arranged by size: 2687 1991 818 529 496 447 91 16 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2498 2591 2909 3293 1163 Size: 573 762 93 318 384 4945 Fragments arranged by size: 4945 762 573 384 318 93 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4525 5286 6314 1540 Size: 275 186 2524 761 1028 2301 Fragments arranged by size: 2524 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2154 3660 3709 3750 5136 5221 6292 2154 Size: 1506 49 41 1386 85 1071 2937 Fragments arranged by size: 2937 1506 1386 1071 85 49 41 BsmI GAATG_Cn' Cuts at: 2119 3834 4586 4679 2119 Size: 1715 752 93 4515 Fragments arranged by size: 4515 1715 752 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2154 2220 2501 2658 2791 3250 3621 Size: 399 364 66 281 157 133 459 371 Cuts at: 3621 3660 3709 3750 4221 4356 4394 5136 5221 Size: 39 49 41 471 135 38 742 85 Cuts at: 5221 6292 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 471 459 399 371 364 281 157 135 133 85 66 49 41 39 38 BspEI T'CCGG_A Cuts at: 3638 6738 3638 Size: 3100 3975 BspHI T'CATG_A Cuts at: 3057 3687 4964 6694 3057 Size: 630 1277 1730 3438 Fragments arranged by size: 3438 1730 1277 630 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3701 4363 4430 844 Size: 2857 662 67 3489 Fragments arranged by size: 3489 2857 662 67 BsrBI CCG'CTC Cuts at: 1394 1823 4962 1394 Size: 429 3139 3507 Fragments arranged by size: 3507 3139 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3854 5570 5744 65 Size: 1183 2606 1716 174 1396 Fragments arranged by size: 2606 1716 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5719 5906 1700 Size: 110 3909 187 2869 Fragments arranged by size: 3909 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2398 96 Size: 2302 4773 BssHII G'CGCG_C Cuts at: 1925 3647 1925 Size: 1722 5353 BssSI C'ACGA_G Cuts at: 5135 6147 5135 Size: 1012 6063 BstAPI GCAn_nnn'nTGC Cuts at: 794 2856 3316 3469 794 Size: 2062 460 153 4400 Fragments arranged by size: 4400 2062 460 153 BstBI TT'CG_AA Cuts at: 3790 5879 3790 Size: 2089 4986 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7075 BstXI CCAn_nnnn'nTGG Cuts at: 4188 4188 Size: 7075 BstYI r'GATC_y Cuts at: 1972 2550 2976 3544 4405 4772 5157 5174 6626 Size: 578 426 568 861 367 385 17 1452 Cuts at: 6626 6712 6724 7070 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 861 578 568 426 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2174 513 Size: 552 1109 5414 Fragments arranged by size: 5414 1109 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4194 487 Size: 683 56 2968 3368 Fragments arranged by size: 3368 2968 683 56 BtsI GCAGTG_nn' Cuts at: 2286 4591 5385 5405 2286 Size: 2305 794 20 3956 Fragments arranged by size: 3956 2305 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7075 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7040 DraI TTT'AAA Cuts at: 4379 4379 Size: 7075 DraIII CAC_nnn'GTG Cuts at: 2201 3469 2201 Size: 1268 5807 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3019 6082 809 Size: 507 1703 3063 1802 Fragments arranged by size: 3063 1802 1703 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2041 2094 3605 Size: 54 1425 50 275 18 211 53 1511 Cuts at: 3605 4150 4522 5411 7040 8 Size: 545 372 889 1629 43 Fragments arranged by size: 1629 1511 1425 889 545 372 275 211 54 53 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4522 1537 Size: 275 2710 4090 Fragments arranged by size: 4090 2710 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2246 3170 3998 5005 1353 Size: 893 924 828 1007 3423 Fragments arranged by size: 3423 1007 924 893 828 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2288 4378 5662 6036 6182 387 Size: 1064 138 699 2090 1284 374 146 1280 Fragments arranged by size: 2090 1284 1280 1064 699 374 146 138 EcoICRI GAG'CTC Cuts at: 2152 2152 Size: 7075 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2396 2669 2849 3050 3299 3425 3635 Size: 393 764 273 180 201 249 126 210 Cuts at: 3635 5138 5735 6522 6790 1239 Size: 1503 597 787 268 1524 Fragments arranged by size: 1524 1503 787 764 597 393 273 268 249 210 201 180 126 EcoNI CCTnn'n_nnAGG Cuts at: 3490 3490 Size: 7075 EcoO109I rG'GnC_Cy Cuts at: 2186 2326 2497 2498 2822 3224 3400 2186 Size: 140 171 1 324 402 176 5861 Fragments arranged by size: 5861 402 324 176 171 140 1 EcoRI G'AATT_C Cuts at: 4384 4762 4384 Size: 378 6697 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2257 2257 Size: 7075 FspI TGC'GCA Cuts at: 5581 5581 Size: 7075 HaeII r_GCGC'y Cuts at: 1678 2954 6222 1678 Size: 1276 3268 2531 Fragments arranged by size: 3268 2531 1276 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2620 2652 3797 3802 3829 Size: 32 135 32 1048 32 1145 5 27 Cuts at: 3829 3834 5716 5748 5790 5822 1373 Size: 5 1882 32 42 32 2626 Fragments arranged by size: 2626 1882 1145 1048 135 42 32 32 32 32 32 27 5 5 HincII GTy'rAC Cuts at: 669 1657 1938 3124 3898 4419 4665 669 Size: 988 281 1186 774 521 246 3079 Fragments arranged by size: 3079 1186 988 774 521 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7075 HpaI GTT'AAC Cuts at: 4665 4665 Size: 7075 KasI G'GCGC_C Cuts at: 2950 2950 Size: 7075 KpnI G_GTAC'C Cuts at: 4400 4400 Size: 7075 MfeI C'AATT_G Cuts at: 4674 4674 Size: 7075 MluI A'CGCG_T Cuts at: 6951 6951 Size: 7075 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2160 3825 6189 6373 1305 Size: 855 1665 2364 184 2007 Fragments arranged by size: 2364 2007 1665 855 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2096 3607 10 Size: 54 1425 343 264 1511 3478 Fragments arranged by size: 3478 1511 1425 343 264 54 MslI CAynn'nnrTG Cuts at: 518 1166 2690 5033 5392 5551 518 Size: 648 1524 2343 359 159 2042 Fragments arranged by size: 2343 2042 1524 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2887 3204 4491 5168 6316 6561 1606 Size: 1281 317 1287 677 1148 245 2120 Fragments arranged by size: 2120 1287 1281 1148 677 317 245 NarI GG'CG_CC Cuts at: 2951 2951 Size: 7075 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6523 NdeI CA'TA_TG Cuts at: 387 387 Size: 7075 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7075 NotI GC'GGCC_GC Cuts at: 4522 4522 Size: 7075 NsiI A_TGCA'T Cuts at: 2861 2861 Size: 7075 NspI r_CATG'y Cuts at: 1132 1229 2395 3556 3889 4433 5978 1132 Size: 97 1166 1161 333 544 1545 2229 Fragments arranged by size: 2229 1545 1166 1161 544 333 97 PasI CC'CwG_GG Cuts at: 1348 3250 1348 Size: 1902 5173 PciI A'CATG_T Cuts at: 5974 5974 Size: 7075 PflMI CCAn_nnn'nTGG Cuts at: 1108 2454 3049 1108 Size: 1346 595 5134 Fragments arranged by size: 5134 1346 595 PfoI T'CCnGG_A Cuts at: 2979 3339 2979 Size: 360 6715 PmeI GTTT'AAAC Cuts at: 4379 4379 Size: 7075 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2825 2857 5144 5176 6657 6689 2825 Size: 32 2287 32 1481 32 3211 Fragments arranged by size: 3211 2287 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2186 2326 2822 3224 3400 2186 Size: 140 496 402 176 5861 Fragments arranged by size: 5861 496 402 176 140 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7075 PsiI TTA'TAA Cuts at: 4645 4645 Size: 7075 PspOMI G'GGCC_C Cuts at: 2497 2497 Size: 7075 PstI C_TGCA'G Cuts at: 830 2145 2680 3954 4374 4427 830 Size: 1315 535 1274 420 53 3478 Fragments arranged by size: 3478 1315 1274 535 420 53 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7075 PvuII CAG'CTG Cuts at: 2887 2887 Size: 7075 SacI G_AGCT'C Cuts at: 2154 2154 Size: 7075 SalI G'TCGA_C Cuts at: 1936 4417 1936 Size: 2481 4594 SanDI GG'GwC_CC Cuts at: 3224 3224 Size: 7075 SapI GCTCTTCn'nnn_ Cuts at: 3170 3170 Size: 7075 SbfI CC_TGCA'GG Cuts at: 4427 4427 Size: 7075 ScaI AGT'ACT Cuts at: 5323 7035 5323 Size: 1712 5363 SfcI C'TryA_G Cuts at: 826 1045 2141 2331 2676 3950 4370 4423 5558 Size: 219 1096 190 345 1274 420 53 1135 Cuts at: 5558 6239 6430 826 Size: 681 191 1471 Fragments arranged by size: 1471 1274 1135 1096 681 420 345 219 191 190 53 SfoI GGC'GCC Cuts at: 2952 2952 Size: 7075 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7075 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7075 SmaI CCC'GGG Cuts at: 1872 4357 4402 1872 Size: 2485 45 4545 Fragments arranged by size: 4545 2485 45 SmlI C'TyrA_G Cuts at: 820 1017 1943 2190 4214 5179 6080 6342 820 Size: 197 926 247 2024 965 901 262 1553 Fragments arranged by size: 2024 1553 965 926 901 262 247 197 SnaBI TAC'GTA Cuts at: 493 4866 493 Size: 4373 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7075 SphI G_CATG'C Cuts at: 1132 2395 3889 4433 1132 Size: 1263 1494 544 3774 Fragments arranged by size: 3774 1494 1263 544 SspI AAT'ATT Cuts at: 5 52 4999 5 Size: 47 4947 2081 Fragments arranged by size: 4947 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7075 StyI C'CwwG_G Cuts at: 513 1065 2157 3066 4514 513 Size: 552 1092 909 1448 3074 Fragments arranged by size: 3074 1448 1092 909 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3643 5102 5119 5272 5457 5902 325 Size: 1255 2063 1459 17 153 185 445 1498 Fragments arranged by size: 2063 1498 1459 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2398 3909 5321 Size: 274 80 33 51 1443 421 1511 1412 Cuts at: 5321 7033 96 Size: 1712 138 Fragments arranged by size: 1712 1511 1443 1412 421 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2267 2299 2900 2932 2961 2993 4161 4193 7035 Size: 32 601 32 29 32 1168 32 2842 Cuts at: 7035 7067 2267 Size: 32 2275 Fragments arranged by size: 2842 2275 1168 601 32 32 32 32 32 29 Tth111I GACn'n_nGTC Cuts at: 1316 3683 1316 Size: 2367 4708 XbaI T'CTAG_A Cuts at: 4411 4411 Size: 7075 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3781 3781 Size: 7075 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7075 XmaI C'CCGG_G Cuts at: 1870 4355 4400 1870 Size: 2485 45 4545 Fragments arranged by size: 4545 2485 45 XmnI GAAnn'nnTTC Cuts at: 5204 5204 Size: 7075 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4883 276 Size: 53 83 186 936 3349 2468 Fragments arranged by size: 3349 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI NaeI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacII SexAI SfiI SrfI SwaI