Restriction map for
pFN21AA1503
(Linear) (Six-base) MAPSORT of: KIAA1503.seq Check: 6584 from: 1 to: 2370
KIAA1503.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AccI GT'mk_AC
Cuts at: 0 416 2370
Size: 416 1954
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 1045 2370
Size: 391 654 1325
Fragments arranged by size:
1325 654 391
AhdI GACnn_n'nnGTC
Cuts at: 0 1811 2370
Size: 1811 559
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 125 2370
Size: 125 2245
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 820 852 2370
Size: 820 32 1518
Fragments arranged by size:
1518 820 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1419 2260 2370
Size: 1419 841 110
Fragments arranged by size:
1419 841 110
ApaI G_GGCC'C
Cuts at: 0 496 2370
Size: 496 1874
ApaLI G'TGCA_C
Cuts at: 0 1651 1741 2370
Size: 1651 90 629
Fragments arranged by size:
1651 629 90
ApoI r'AATT_y
Cuts at: 0 810 1960 2370
Size: 810 1150 410
Fragments arranged by size:
1150 810 410
AvaI C'yCGr_G
Cuts at: 0 77 214 245 1139 2350 2370
Size: 77 137 31 894 1211 20
Fragments arranged by size:
1211 894 137 77 31 20
BanI G'GyrC_C
Cuts at: 0 945 1240 2370
Size: 945 295 1130
Fragments arranged by size:
1130 945 295
BanII G_rGCy'C
Cuts at: 0 149 215 496 1616 2216 2351 2370
Size: 149 66 281 1120 600 135 19
Fragments arranged by size:
1120 600 281 149 135 66 19
BbeI G_GCGC'C
Cuts at: 0 949 2370
Size: 949 1421
BbsI GAAGACnn'nnnn_
Cuts at: 0 1370 2370
Size: 1370 1000
BbvCI CC'TCA_GC
Cuts at: 0 1428 2370
Size: 1428 942
BciVI GTATCCnnnnn_n'
Cuts at: 0 1342 1836 2370
Size: 1342 494 534
Fragments arranged by size:
1342 534 494
BclI T'GATC_A
Cuts at: 0 1091 1421 2370
Size: 1091 330 949
Fragments arranged by size:
1091 949 330
BfrBI ATG'CAT
Cuts at: 0 854 2370
Size: 854 1516
BglI GCCn_nnn'nGGC
Cuts at: 0 79 2355 2370
Size: 79 2276 15
Fragments arranged by size:
2276 79 15
BglII A'GATC_T
Cuts at: 0 545 2370
Size: 545 1825
BlpI GC'TnA_GC
Cuts at: 0 143 2370
Size: 143 2227
Bme1580I G_kGCm'C
Cuts at: 0 496 653 786 1245 1655 1745 2370
Size: 496 157 133 459 410 90 625
Fragments arranged by size:
625 496 459 410 157 133 90
BmgBI CAC'GTC
Cuts at: 0 1452 2370
Size: 1452 918
BmrI ACTGGGnnnn_n'
Cuts at: 0 1620 2248 2370
Size: 1620 628 122
Fragments arranged by size:
1620 628 122
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 664 844 1294 1420 1630 2370
Size: 664 180 450 126 210 740
Fragments arranged by size:
740 664 450 210 180 126
Bpu10I CC'TnA_GC
Cuts at: 0 164 1085 1428 2345 2370
Size: 164 921 343 917 25
Fragments arranged by size:
921 917 343 164 25
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 2230 2370
Size: 170 2060 140
Fragments arranged by size:
2060 170 140
BsaI GGTCTCn'nnnn_
Cuts at: 0 730 2313 2370
Size: 730 1583 57
Fragments arranged by size:
1583 730 57
BsaAI yAC'GTr
Cuts at: 0 1620 2370
Size: 1620 750
BsaHI Gr'CG_yC
Cuts at: 0 946 1665 2370
Size: 946 719 705
Fragments arranged by size:
946 719 705
BsaWI w'CCGG_w
Cuts at: 0 1633 2370
Size: 1633 737
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 820 850 2370
Size: 820 30 1520
Fragments arranged by size:
1520 820 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 172 841 1973 2370
Size: 172 669 1132 397
Fragments arranged by size:
1132 669 397 172
BseYI C'CCAG_C
Cuts at: 0 381 1199 1646 2175 2191 2282 2370
Size: 381 818 447 529 16 91 88
Fragments arranged by size:
818 529 447 381 91 88 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 586 904 1288 2370
Size: 493 93 318 384 1082
Fragments arranged by size:
1082 493 384 318 93
BsiHKAI G_wGCw'C
Cuts at: 0 149 1655 1704 1745 2370
Size: 149 1506 49 41 625
Fragments arranged by size:
1506 625 149 49 41
BsmI GAATG_Cn'
Cuts at: 0 114 1829 2370
Size: 114 1715 541
Fragments arranged by size:
1715 541 114
Bsp1286I G_dGCh'C
Cuts at: 0 149 215 496 653 786 1245 1616 1655
Size: 149 66 281 157 133 459 371 39
Cuts at: 1655 1704 1745 2216 2351 2370
Size: 49 41 471 135 19
Fragments arranged by size:
471 459 371 281 157 149 135 133
66 49 41 39 19
BspEI T'CCGG_A
Cuts at: 0 1633 2370
Size: 1633 737
BspHI T'CATG_A
Cuts at: 0 1052 1682 2370
Size: 1052 630 688
Fragments arranged by size:
1052 688 630
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1696 2370
Size: 1696 674
BsrDI GCAATG_nn'
Cuts at: 0 1849 2370
Size: 1849 521
BsrGI T'GTAC_A
Cuts at: 0 393 2370
Size: 393 1977
BssHII G'CGCG_C
Cuts at: 0 1642 2370
Size: 1642 728
BstAPI GCAn_nnn'nTGC
Cuts at: 0 851 1311 1464 2370
Size: 851 460 153 906
Fragments arranged by size:
906 851 460 153
BstBI TT'CG_AA
Cuts at: 0 1785 2370
Size: 1785 585
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2183 2370
Size: 2183 187
BstYI r'GATC_y
Cuts at: 0 545 971 1539 2370
Size: 545 426 568 831
Fragments arranged by size:
831 568 545 426
BtgI C'CryG_G
Cuts at: 0 169 2370
Size: 169 2201
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2189 2370
Size: 2189 181
BtsI GCAGTG_nn'
Cuts at: 0 281 2370
Size: 281 2089
DraIII CAC_nnn'GTG
Cuts at: 0 196 1464 2370
Size: 196 1268 906
Fragments arranged by size:
1268 906 196
DrdI GACnn_nn'nnGTC
Cuts at: 0 1014 2370
Size: 1014 1356
EaeI y'GGCC_r
Cuts at: 0 36 89 1600 2145 2370
Size: 36 53 1511 545 225
Fragments arranged by size:
1511 545 225 53 36
EarI CTCTTCn'nnn_
Cuts at: 0 241 1165 1993 2370
Size: 241 924 828 377
Fragments arranged by size:
924 828 377 241
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 283 2370
Size: 283 2087
EcoICRI GAG'CTC
Cuts at: 0 147 2370
Size: 147 2223
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 664 844 1045 1294 1420 1630 2370
Size: 391 273 180 201 249 126 210 740
Fragments arranged by size:
740 391 273 249 210 201 180 126
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1485 2370
Size: 1485 885
EcoO109I rG'GnC_Cy
Cuts at: 0 181 321 492 493 817 1219 1395 2370
Size: 181 140 171 1 324 402 176 975
Fragments arranged by size:
975 402 324 181 176 171 140 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 252 2370
Size: 252 2118
HaeII r_GCGC'y
Cuts at: 0 949 2370
Size: 949 1421
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 615 647 1792 1797 1824 1829 2370
Size: 615 32 1145 5 27 5 541
Fragments arranged by size:
1145 615 541 32 27 5 5
HincII GTy'rAC
Cuts at: 0 1119 1893 2370
Size: 1119 774 477
Fragments arranged by size:
1119 774 477
KasI G'GCGC_C
Cuts at: 0 945 2370
Size: 945 1425
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 1820 2370
Size: 155 1665 550
Fragments arranged by size:
1665 550 155
MscI TGG'CCA
Cuts at: 0 91 1602 2370
Size: 91 1511 768
Fragments arranged by size:
1511 768 91
MslI CAynn'nnrTG
Cuts at: 0 685 2370
Size: 685 1685
MspA1I CmG'CkG
Cuts at: 0 882 1199 2370
Size: 882 317 1171
Fragments arranged by size:
1171 882 317
NarI GG'CG_CC
Cuts at: 0 946 2370
Size: 946 1424
NsiI A_TGCA'T
Cuts at: 0 856 2370
Size: 856 1514
NspI r_CATG'y
Cuts at: 0 390 1551 1884 2370
Size: 390 1161 333 486
Fragments arranged by size:
1161 486 390 333
PasI CC'CwG_GG
Cuts at: 0 1245 2370
Size: 1245 1125
PflMI CCAn_nnn'nTGG
Cuts at: 0 449 1044 2370
Size: 449 595 1326
Fragments arranged by size:
1326 595 449
PfoI T'CCnGG_A
Cuts at: 0 974 1334 2370
Size: 974 360 1036
Fragments arranged by size:
1036 974 360
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 820 852 2370
Size: 820 32 1518
Fragments arranged by size:
1518 820 32
PpuMI rG'GwC_Cy
Cuts at: 0 181 321 817 1219 1395 2370
Size: 181 140 496 402 176 975
Fragments arranged by size:
975 496 402 181 176 140
PspOMI G'GGCC_C
Cuts at: 0 492 2370
Size: 492 1878
PstI C_TGCA'G
Cuts at: 0 140 675 1949 2369 2370
Size: 140 535 1274 420 1
Fragments arranged by size:
1274 535 420 140 1
PvuII CAG'CTG
Cuts at: 0 882 2370
Size: 882 1488
SacI G_AGCT'C
Cuts at: 0 149 2370
Size: 149 2221
SanDI GG'GwC_CC
Cuts at: 0 1219 2370
Size: 1219 1151
SapI GCTCTTCn'nnn_
Cuts at: 0 1165 2370
Size: 1165 1205
SfcI C'TryA_G
Cuts at: 0 136 326 671 1945 2365 2370
Size: 136 190 345 1274 420 5
Fragments arranged by size:
1274 420 345 190 136 5
SfoI GGC'GCC
Cuts at: 0 947 2370
Size: 947 1423
SmaI CCC'GGG
Cuts at: 0 2352 2370
Size: 2352 18
SmlI C'TyrA_G
Cuts at: 0 185 2209 2370
Size: 185 2024 161
Fragments arranged by size:
2024 185 161
SphI G_CATG'C
Cuts at: 0 390 1884 2370
Size: 390 1494 486
Fragments arranged by size:
1494 486 390
StyI C'CwwG_G
Cuts at: 0 152 1061 2370
Size: 152 909 1309
Fragments arranged by size:
1309 909 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1638 2370
Size: 1638 732
TatI w'GTAC_w
Cuts at: 0 393 1904 2370
Size: 393 1511 466
Fragments arranged by size:
1511 466 393
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 262 294 895 927 956 988 2156 2188
Size: 262 32 601 32 29 32 1168 32
Cuts at: 2188 2370
Size: 182
Fragments arranged by size:
1168 601 262 182 32 32 32 32
29
Tth111I GACn'n_nGTC
Cuts at: 0 1678 2370
Size: 1678 692
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1776 2370
Size: 1776 594
XmaI C'CCGG_G
Cuts at: 0 2350 2370
Size: 2350 20
Enzymes that do cut:
AccI AcuI AhdI AlfI AloI AlwNI ApaI ApaLI
ApoI AvaI BanI BanII BbeI BbsI BbvCI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrDI BsrGI BssHII BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraIII DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII
KasI MmeI MscI MslI MspA1I NarI NsiI NspI
PasI PflMI PfoI PpiI PpuMI PspOMI PstI PvuII
SacI SanDI SapI SfcI SfoI SmaI SmlI SphI
StyI TaqII TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AleI AscI AseI AsiSI AvrII BaeI BamHI BcgI
BmtI BplI BsaBI BsiEI BsiWI BsmBI BsrBI BsrFI
BssSI BstEII BstZ17I Bsu36I ClaI CspCI DraI EagI
EcoRI EcoRV FseI FspI FspAI HindIII HpaI KpnI
MfeI MluI NaeI NcoI NdeI NgoMIV NheI NotI
NruI PacI PciI PmeI PmlI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SbfI ScaI SexAI
SfiI SgfI SgrAI SnaBI SpeI SrfI SspI StuI
SwaI XbaI XhoI XmnI ZraI