Restriction map for pFN21AA0896
 (Circular) (Six-base) MAPSORT of: pFN21AA0896.seq  Check: 7350  from: 1  to: 13099



pFN21AA0896  13099 bp

 With 182 enzymes: SgfI * 

                        March 28, 2012 14:41  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   3461    3461 
   Size:      13099
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2012    2309   10909     278 
   Size:         53      83     186     936     476     297    8600    2468

  Fragments arranged by size: 

                8600    2468     936     476     297     186      83      53
            
 
AccI GT'mk_AC

Cuts at:   1937    4222    4669    5011    6111    7431    8107    9405    9973 
   Size:       2285     447     342    1100    1320     676    1298     568

Cuts at:   9973   10442    1937 
   Size:        469    4594

  Fragments arranged by size: 

                4594    2285    1320    1298    1100     676     568     469
                 447     342
 
Acc65I G'GTAC_C

Cuts at:   3596    4454    5489    7271   10420    3596 
   Size:        858    1035    1782    3149    6275

  Fragments arranged by size: 

                6275    3149    1782    1035     858
 
AclI AA'CG_TT

Cuts at:   1563   11226   11599    1563 
   Size:       9663     373    3063

  Fragments arranged by size: 

                9663    3063     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2615    2841    3801    4304    5952    7011    7572    8160 
   Size:        983     226     960     503    1648    1059     561     588

Cuts at:   8160    9162   11162   12546   12814    1632 
   Size:       1002    2000    1384     268    1917

  Fragments arranged by size: 

                2000    1917    1648    1384    1059    1002     983     960
                 588     561     503     268     226
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197   12902
 
AflIII A'CryG_T

Cuts at:   1203    3851    9658   11998   12975    1203 
   Size:       2648    5807    2340     977    1327

  Fragments arranged by size: 

                5807    2648    2340    1327     977
 
AgeI A'CCGG_T

Cuts at:   5168   11930    5168 
   Size:       6762    6337
 
AhdI GACnn_n'nnGTC

Cuts at:  10319   11828   10319 
   Size:       1509   11590
 
AleI CACnn'nnGTG

Cuts at:   1166    2787    4749    1166 
   Size:       1621    1962    9516

  Fragments arranged by size: 

                9516    1962    1621
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    8199    9720   10318    1184 
   Size:       7015    1521     598    3965

  Fragments arranged by size: 

                7015    3965    1521     598
 
AlwNI CAG_nnn'CTG

Cuts at:   2354    2552    4100    4726    6863    6944    7304    7451    8051 
   Size:        198    1548     626    2137      81     360     147     600

Cuts at:   8051    8627    9140    9773   10842   12414    2354 
   Size:        576     513     633    1069    1572    3039

  Fragments arranged by size: 

                3039    2137    1572    1548    1069     633     626     600
                 576     513     360     198     147      81
 
ApaLI G'TGCA_C

Cuts at:  11156   12312   11156 
   Size:       1156   11943
 
ApoI r'AATT_y

Cuts at:   1493    3113    4046    7633    8675    9994   10384   10408   10634 
   Size:       1620     933    3587    1042    1319     390      24     226

Cuts at:  10634   10786   12895    1493 
   Size:        152    2109    1697

  Fragments arranged by size: 

                3587    2109    1697    1620    1319    1042     933     390
                 226     152      24
 
AseI AT'TA_AT

Cuts at:    160    1032   11653     160 
   Size:        872   10621    1606

  Fragments arranged by size: 

               10621    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:      13099
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2290    2457    2478    2886   10424    1870 
   Size:         73     347     167      21     408    7538    4545

  Fragments arranged by size: 

                7538    4545     408     347     167      73      21
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   3588    3621    6204    6237    3588 
   Size:         33    2583      33   10450

  Fragments arranged by size: 

               10450    2583      33      33
 
BamHI G'GATC_C

Cuts at:   2429    4498    5047    5104   10429   10796    2429 
   Size:       2069     549      57    5325     367    4732

  Fragments arranged by size: 

                5325    4732    2069     549     367      57
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2042    3596    4028    4454    5489 
   Size:        325     217     627     255    1554     432     426    1035

Cuts at:   5489    7271    8571    8966   10420   11875     618 
   Size:       1782    1300     395    1454    1455    1842

  Fragments arranged by size: 

                1842    1782    1554    1455    1454    1300    1035     627
                 432     426     395     325     255     217
 
BanII G_rGCy'C

Cuts at:   1391    2458    3133   10418    1391 
   Size:       1067     675    7285    4072

  Fragments arranged by size: 

                7285    4072    1067     675
 
BbeI G_GCGC'C

Cuts at:   8575    8575 
   Size:      13099
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2829    4292    6831    9106     928 
   Size:        971     930    1463    2539    2275    4921

  Fragments arranged by size: 

                4921    2539    2275    1463     971     930
 
BbvCI CC'TCA_GC

Cuts at:   6792    6792 
   Size:      13099
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937   11290   11324    1903 
   Size:         34    9353      34    3678

  Fragments arranged by size: 

                9353    3678      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   7900    8957    9395   10991   12201    7900 
   Size:       1057     438    1596    1210    8798

  Fragments arranged by size: 

                8798    1596    1210    1057     438
 
BclI T'GATC_A

Cuts at:   1548    6580    8158   10071    1548 
   Size:       5032    1578    1913    4576

  Fragments arranged by size: 

                5032    4576    1913    1578
 
BfrBI ATG'CAT

Cuts at:   3863    3863 
   Size:      13099
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672   11710     136 
   Size:        107     122      71     631     605   10038    1525

  Fragments arranged by size: 

               10038    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   3734    8749   10183   13094    3734 
   Size:       5015    1434    2911    3739

  Fragments arranged by size: 

                5015    3739    2911    1434
 
BlpI GC'TnA_GC

Cuts at:   3459    3786    7715    9282   10516    3459 
   Size:        327    3929    1567    1234    6042

  Fragments arranged by size: 

                6042    3929    1567    1234     327
 
Bme1580I G_kGCm'C

Cuts at:   1790    2045    3445   11160   12316    1790 
   Size:        255    1400    7715    1156    2573

  Fragments arranged by size: 

                7715    2573    1400    1156     255
 
BmgBI CAC'GTC

Cuts at:   1318    7703    1318 
   Size:       6385    6714
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    7961   10830   11788 
   Size:        416      53      83     399      24    6544    2869     958

Cuts at:  11788     442 
   Size:       1753

  Fragments arranged by size: 

                6544    2869    1753     958     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    3771    7320    7677    1056 
   Size:       2715    3549     357    6478

  Fragments arranged by size: 

                6478    3549    2715     357
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2932    5843    8771    2932 
   Size:       2911    2928    7260

  Fragments arranged by size: 

                7260    2928    2911
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2419    2427    2472    2757    3657    3825    4045    5043 
   Size:       1180       8      45     285     900     168     220     998

Cuts at:   5043    5282    5577    5630    6580    6762   11759    1239 
   Size:        239     295      53     950     182    4997    2579

  Fragments arranged by size: 

                4997    2579    1180     998     950     900     295     285
                 239     220     182     168      53      45       8
 
Bpu10I CC'TnA_GC

Cuts at:   6792    9165    6792 
   Size:       2373   10726
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2210    3631    4709    5425    7000   11224   12089   12387    2210 
   Size:       1421    1078     716    1575    4224     865     298    2922

  Fragments arranged by size: 

                4224    2922    1575    1421    1078     865     716     298
            
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3963    7058    7788    9006   11762     882 
   Size:        617    2464    3095     730    1218    2756    2219

  Fragments arranged by size: 

                3095    2756    2464    2219    1218     730     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2957    3852   10890     493 
   Size:        711    1753     895    7038    2702

  Fragments arranged by size: 

                7038    2702    1753     895     711
 
BsaBI GATnn'nnATC

Cuts at:   4758    8742    8748   13093    4758 
   Size:       3984       6    4345    4764

  Fragments arranged by size: 

                4764    4345    3984       6
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2009    2306    8572   10906 
   Size:         53      83     186     936     476     297    6266    2334

Cuts at:  10906   11288   11923     275 
   Size:        382     635    1451

  Fragments arranged by size: 

                6266    2334    1451     936     635     476     382     297
                 186      83      53
 
BsaWI w'CCGG_w

Cuts at:   5168   11532   11930   12204   12351   12762    5168 
   Size:       6364     398     274     147     411    5505

  Fragments arranged by size: 

                6364    5505     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    5603    5633    9037    9040    9067 
   Size:         30     165      30    4200      30    3404       3      27

Cuts at:   9067    9070    9828    9858    1178 
   Size:          3     758      30    4419

  Fragments arranged by size: 

                4419    4200    3404     758     165      30      30      30
                  30      27       3       3
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2579    7761    9018    9058    9061    9400    1186 
   Size:       1393    5182    1257      40       3     339    4885

  Fragments arranged by size: 

                5182    4885    1393    1257     339      40       3
 
BseYI C'CCAG_C

Cuts at:   2532    3662    5979    7167    9277   12302   12798    2532 
   Size:       1130    2317    1188    2110    3025     496    2833

  Fragments arranged by size: 

                3025    2833    2317    2110    1188    1130     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    3903    6471    7725    8424    1163 
   Size:        573    2167    2568    1254     699    5838

  Fragments arranged by size: 

                5838    2568    2167    1254     699     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001   10549   11310   12338    1540 
   Size:        275     186    8548     761    1028    2301

  Fragments arranged by size: 

                8548    2301    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2458    3384    5568    5826   11160   11245   12316    2458 
   Size:        926    2184     258    5334      85    1071    3241

  Fragments arranged by size: 

                5334    3241    2184    1071     926     258      85
 
BsmI GAATG_Cn'

Cuts at:   4549    5260    6019    7798   10610   10703    4549 
   Size:        711     759    1779    2812      93    6945

  Fragments arranged by size: 

                6945    2812    1779     759     711      93
 
BsmBI CGTCTCn'nnnn_

Cuts at:   4975    9762    4975 
   Size:       4787    8312
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2045    2458    3133    3384    3445    5568    5826 
   Size:        399     255     413     675     251      61    2123     258

Cuts at:   5826   10418   11160   11245   12316    1391 
   Size:       4592     742      85    1071    2174

  Fragments arranged by size: 

                4592    2174    2123    1071     742     675     413     399
                 258     255     251      85      61
 
BspEI T'CCGG_A

Cuts at:  12762   12762 
   Size:      13099
 
BspHI T'CATG_A

Cuts at:   5659    5953    6757    8428   10988   12718    5659 
   Size:        294     804    1671    2560    1730    6040

  Fragments arranged by size: 

                6040    2560    1730    1671     804     294
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3461    4144    9056   10454     844 
   Size:       2617     683    4912    1398    3489

  Fragments arranged by size: 

                4912    3489    2617    1398     683
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3418   10986    1394 
   Size:        429    1595    7568    3507

  Fragments arranged by size: 

                7568    3507    1595     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3433    4368    4887    6157    6401    7890    8898 
   Size:       1183    2185     935     519    1270     244    1489    1008

Cuts at:   8898    9231    9798    9966   11594   11768      65 
   Size:        333     567     168    1628     174    1396

  Fragments arranged by size: 

                2185    1628    1489    1396    1270    1183    1008     935
                 567     519     333     244     174     168
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2469    5168    8352   11743   11930    1700 
   Size:        110     659    2699    3184    3391     187    2869

  Fragments arranged by size: 

                3391    3184    2869    2699     659     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    6176    8824    9217      96 
   Size:       6080    2648     393    3978

  Fragments arranged by size: 

                6080    3978    2648     393
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:      13099
 
BssSI C'ACGA_G

Cuts at:   3240    7743    9213   11159   12171    3240 
   Size:       4503    1470    1946    1012    4168

  Fragments arranged by size: 

                4503    4168    1946    1470    1012
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    6944    7304     794 
   Size:       6150     360    6589

  Fragments arranged by size: 

                6589    6150     360
 
BstBI TT'CG_AA

Cuts at:  11903   11903 
   Size:      13099
 
BstEII G'GTnAC_C

Cuts at:   1184    2840    1184 
   Size:       1656   11443
 
BstXI CCAn_nnnn'nTGG

Cuts at:   7118    8152    7118 
   Size:       1034   12065
 
BstYI r'GATC_y

Cuts at:   1972    2429    2743    3734    4498    4513    5047    5104    5147 
   Size:        457     314     991     764      15     534      57      43

Cuts at:   5147    8197    8749    9391   10183   10429   10796   11181   11198 
   Size:       3050     552     642     792     246     367     385      17

Cuts at:  11198   12650   12736   12748   13094    1972 
   Size:       1452      86      12     346    1977

  Fragments arranged by size: 

                3050    1977    1452     991     792     764     642     552
                 534     457     385     367     346     314     246      86
                  57      43      17      15      12
 
BstZ17I GTA'TAC

Cuts at:   4223    6112    7432    8108    4223 
   Size:       1889    1320     676    9214

  Fragments arranged by size: 

                9214    1889    1320     676
 
Bsu36I CC'TnA_GG

Cuts at:   2386    2386 
   Size:      13099
 
BtgI C'CryG_G

Cuts at:    513    1065    2336    9339    9442     513 
   Size:        552    1271    7003     103    4170

  Fragments arranged by size: 

                7003    4170    1271     552     103
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    5114    9171     487 
   Size:        683      56    3888    4057    4415

  Fragments arranged by size: 

                4415    4057    3888     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3092    3384    3886    3959    4254    4410    5781    6538    6933 
   Size:        292     502      73     295     156    1371     757     395

Cuts at:   6933    6976    7154    8875   10214   10615   11409   11429    3092 
   Size:         43     178    1721    1339     401     794      20    4762

  Fragments arranged by size: 

                4762    1721    1371    1339     794     757     502     401
                 395     295     292     178     156      73      43      20
            
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:      13099
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    4150    4185    8154    8189     529 
   Size:         35    3586      35    3969      35    5439

  Fragments arranged by size: 

                5439    3969    3586      35      35      35
 
DraI TTT'AAA

Cuts at:   2106   10403    2106 
   Size:       8297    4802
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316   12106     809 
   Size:        507   10790    1802

  Fragments arranged by size: 

               10790    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    4470    5371    8053 
   Size:         54    1425      50     275      18    2640     901    2682

Cuts at:   8053    8206   10546   11435   13064       8 
   Size:        153    2340     889    1629      43

  Fragments arranged by size: 

                2682    2640    2340    1629    1425     901     889     275
                 153      54      50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812   10546    1537 
   Size:        275    8734    4090

  Fragments arranged by size: 

                8734    4090     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3924    3942    3966    4821    6096    6708    6741    7623 
   Size:       2571      18      24     855    1275     612      33     882

Cuts at:   7623    7639    8043   11029    1353 
   Size:         16     404    2986    3423

  Fragments arranged by size: 

                3423    2986    2571    1275     882     855     612     404
                  33      24      18      16
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589   11686   12060   12206     387 
   Size:       1064     138   10097     374     146    1280

  Fragments arranged by size: 

               10097    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2456    2456 
   Size:      13099
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2419    2427    2472    2615    2757    2841    3657 
   Size:        393     787       8      45     143     142      84     816

Cuts at:   3657    3801    3825    4045    4304    5043    5282    5577    5630 
   Size:        144      24     220     259     739     239     295      53

Cuts at:   5630    5952    6580    6762    7011    7572    8160    9162   11162 
   Size:        322     628     182     249     561     588    1002    2000

Cuts at:  11162   11759   12546   12814    1239 
   Size:        597     787     268    1524

  Fragments arranged by size: 

                2000    1524    1002     816     787     787     739     628
                 597     588     561     393     322     295     268     259
                 249     239     220     182     144     143     142      84
                  53      45      24       8
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2437    2437 
   Size:      13099
 
EcoO109I rG'GnC_Cy

Cuts at:   3827    5207    9123    3827 
   Size:       1380    3916    7803

  Fragments arranged by size: 

                7803    3916    1380
 
EcoRI G'AATT_C

Cuts at:   7633   10408   10786    7633 
   Size:       2775     378    9946

  Fragments arranged by size: 

                9946    2775     378
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3188    6080    7523    8028    9692    3188 
   Size:       2892    1443     505    1664    6595

  Fragments arranged by size: 

                6595    2892    1664    1443     505
 
FspI TGC'GCA

Cuts at:  11605   11605 
   Size:      13099
 
HaeII r_GCGC'y

Cuts at:   1678    7722    8575    9442   12246    1678 
   Size:       6044     853     867    2804    2531

  Fragments arranged by size: 

                6044    2804    2531     867     853
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2492    2524    2626    2658    2735 
   Size:         32     135      32     920      32     102      32      77

Cuts at:   2735    2767    2966    2998    3604    3636    3851    3883    3941 
   Size:         32     199      32     606      32     215      32      58

Cuts at:   3941    3973    4296    4328    6463    6495    6989    7021    7814 
   Size:         32     323      32    2135      32     494      32     793

Cuts at:   7814    7846    8018    8050    8823    8855    9193    9225    9955 
   Size:         32     172      32     773      32     338      32     730

Cuts at:   9955    9987   10305   10337   11740   11772   11814   11846    1373 
   Size:         32     318      32    1403      32      42      32    2626

  Fragments arranged by size: 

                2626    2135    1403     920     793     773     730     606
                 494     338     323     318     215     199     172     135
                 102      77      58      42      32      32      32      32
                  32      32      32      32      32      32      32      32
                  32      32      32      32      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3261    3912    4670   10443   10689     669 
   Size:        988     281    1323     651     758    5773     246    3079

  Fragments arranged by size: 

                5773    3079    1323     988     758     651     281     246
            
 
HindIII A'AGCT_T

Cuts at:    748    3745    4214    7505   10118     748 
   Size:       2997     469    3291    2613    3729

  Fragments arranged by size: 

                3729    3291    2997    2613     469
 
HpaI GTT'AAC

Cuts at:   3261   10689    3261 
   Size:       7428    5671
 
KasI G'GCGC_C

Cuts at:   8571    8571 
   Size:      13099
 
KpnI G_GTAC'C

Cuts at:   3600    4458    5493    7275   10424    3600 
   Size:        858    1035    1782    3149    6275

  Fragments arranged by size: 

                6275    3149    1782    1035     858
 
MfeI C'AATT_G

Cuts at:   3870    6372    9691    9810   10698    3870 
   Size:       2502    3319     119     888    6271

  Fragments arranged by size: 

                6271    3319    2502     888     119
 
MluI A'CGCG_T

Cuts at:  12975   12975 
   Size:      13099
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4823    6506    8098    8324    8596    9246   12213   12397 
   Size:       3518    1683    1592     226     272     650    2967     184

Cuts at:  12397    1305 
   Size:       2007

  Fragments arranged by size: 

                3518    2967    2007    1683    1592     650     272     226
                 184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    4472    8055      10 
   Size:         54    1425     343    2640    3583    5054

  Fragments arranged by size: 

                5054    3583    2640    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2787    3438    3850    4749    4779    5471    6759 
   Size:        648    1621     651     412     899      30     692    1288

Cuts at:   6759    7116    7791    7803    7983    9257   11057   11416   11575 
   Size:        357     675      12     180    1274    1800     359     159

Cuts at:  11575     518 
   Size:       2042

  Fragments arranged by size: 

                2042    1800    1621    1288    1274     899     692     675
                 651     648     412     359     357     180     159      30
                  12
 
MspA1I CmG'CkG

Cuts at:   1606    2035    2397    2450    3463    3717    5000    5141    5363 
   Size:        429     362      53    1013     254    1283     141     222

Cuts at:   5363    6863    7229    7295    7304    7451    9286   10515   11192 
   Size:       1500     366      66       9     147    1835    1229     677

Cuts at:  11192   12340   12585    1606 
   Size:       1148     245    2120

  Fragments arranged by size: 

                2120    1835    1500    1283    1229    1148    1013     677
                 429     366     362     254     245     222     147     141
                  66      53       9
 
NaeI GCC'GGC

Cuts at:   2471    2471 
   Size:      13099
 
NarI GG'CG_CC

Cuts at:   8572    8572 
   Size:      13099
 
NcoI C'CATG_G

Cuts at:    513    1065    2336    9339    9442     513 
   Size:        552    1271    7003     103    4170

  Fragments arranged by size: 

                7003    4170    1271     552     103
 
NdeI CA'TA_TG

Cuts at:    387    7849     387 
   Size:       7462    5637
 
NgoMIV G'CCGG_C

Cuts at:   2469    2469 
   Size:      13099
 
NheI G'CTAG_C

Cuts at:   1052    3767    7316    7673    1052 
   Size:       2715    3549     357    6478

  Fragments arranged by size: 

                6478    3549    2715     357
 
NotI GC'GGCC_GC

Cuts at:  10546   10546 
   Size:      13099
 
NsiI A_TGCA'T

Cuts at:   3865    3865 
   Size:      13099
 
NspI r_CATG'y

Cuts at:   1132    1229    4778    7844    8347    9262    9662    9959   10457 
   Size:         97    3549    3066     503     915     400     297     498

Cuts at:  10457   12002    1132 
   Size:       1545    2229

  Fragments arranged by size: 

                3549    3066    2229    1545     915     503     498     400
                 297      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:      13099
 
PciI A'CATG_T

Cuts at:   9658   11998    9658 
   Size:       2340   10759
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2023    9554    1108 
   Size:        915    7531    4653

  Fragments arranged by size: 

                7531    4653     915
 
PfoI T'CCnGG_A

Cuts at:   4051    7597    9387    4051 
   Size:       3546    1790    7763

  Fragments arranged by size: 

                7763    3546    1790
 
PmeI GTTT'AAAC

Cuts at:  10403   10403 
   Size:      13099
 
PmlI CAC'GTG

Cuts at:   3852    3852 
   Size:      13099
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2273    2305   11168   11200   12681   12713    2273 
   Size:         32    8863      32    1481      32    2659

  Fragments arranged by size: 

                8863    2659    1481      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   3827    5207    3827 
   Size:       1380   11719
 
PshAI GACnn'nnGTC

Cuts at:   1613    3907    1613 
   Size:       2294   10805
 
PsiI TTA'TAA

Cuts at:  10669   10669 
   Size:      13099
 
PspXI vC'TCGA_Gb

Cuts at:   2478    2478 
   Size:      13099
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   9936    9968    9936 
   Size:         32   13067
 
PstI C_TGCA'G

Cuts at:    830    4122    4731    6940    7294    7300    8047   10128   10451 
   Size:       3292     609    2209     354       6     747    2081     323

Cuts at:  10451     830 
   Size:       3478

  Fragments arranged by size: 

                3478    3292    2209    2081     747     609     354     323
                   6
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:      13099
 
PvuII CAG'CTG

Cuts at:   2450    3463    3717    5000    5363    6863    7295    7304    7451 
   Size:       1013     254    1283     363    1500     432       9     147

Cuts at:   7451    9286    2450 
   Size:       1835    6263

  Fragments arranged by size: 

                6263    1835    1500    1283    1013     432     363     254
                 147       9
 
SacI G_AGCT'C

Cuts at:   2458    2458 
   Size:      13099
 
SalI G'TCGA_C

Cuts at:   1936    4668   10441    1936 
   Size:       2732    5773    4594

  Fragments arranged by size: 

                5773    4594    2732
 
SapI GCTCTTCn'nnn_

Cuts at:   3942    6096    3942 
   Size:       2154   10945
 
SbfI CC_TGCA'GG

Cuts at:   4122   10451    4122 
   Size:       6329    6770
 
ScaI AGT'ACT

Cuts at:   6508   11347   13059    6508 
   Size:       4839    1712    6548

  Fragments arranged by size: 

                6548    4839    1712
 
SfcI C'TryA_G

Cuts at:    826    1045    3309    3315    3718    4118    4326    4614    4718 
   Size:        219    2264       6     403     400     208     288     104

Cuts at:   4718    4727    5226    6002    6936    7290    7296    8043   10124 
   Size:          9     499     776     934     354       6     747    2081

Cuts at:  10124   10447   11582   12263   12454     826 
   Size:        323    1135     681     191    1471

  Fragments arranged by size: 

                2264    2081    1471    1135     934     776     747     681
                 499     403     400     354     323     288     219     208
                 191     104       9       6       6
 
SfoI GGC'GCC

Cuts at:   8573    8573 
   Size:      13099
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:      13099
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:      13099
 
SmaI CCC'GGG

Cuts at:   1872   10426    1872 
   Size:       8554    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2189    2457    2478    2886    3610    4688 
   Size:        197     926     246     268      21     408     724    1078

Cuts at:   4688    5440    7015   11203   12104   12366     820 
   Size:        752    1575    4188     901     262    1553

  Fragments arranged by size: 

                4188    1575    1553    1078     926     901     752     724
                 408     268     262     246     197      21
 
SnaBI TAC'GTA

Cuts at:    493   10890     493 
   Size:      10397    2702
 
SpeI A'CTAG_T

Cuts at:    152    6217     152 
   Size:       6065    7034
 
SphI G_CATG'C

Cuts at:   1132    4778   10457    1132 
   Size:       3646    5679    3774

  Fragments arranged by size: 

                5679    3774    3646
 
SspI AAT'ATT

Cuts at:      5      52    4242    4768   11023       5 
   Size:         47    4190     526    6255    2081

  Fragments arranged by size: 

                6255    4190    2081     526      47
 
StuI AGG'CCT

Cuts at:   1517    2314    4664    9553    1517 
   Size:        797    2350    4889    5063

  Fragments arranged by size: 

                5063    4889    2350     797
 
StyI C'CwwG_G

Cuts at:    513    1065    2336    4378    5238    5328    8098    8151    9339 
   Size:        552    1271    2042     860      90    2770      53    1188

Cuts at:   9339    9442    9548   10538     513 
   Size:        103     106     990    3074

  Fragments arranged by size: 

                3074    2770    2042    1271    1188     990     860     552
                 106     103      90      53
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2402    2799    3331    4532    7181    8091    8639 
   Size:       1255     822     397     532    1201    2649     910     548

Cuts at:   8639    8984   11126   11143   11296   11481   11926     325 
   Size:        345    2142      17     153     185     445    1498

  Fragments arranged by size: 

                2649    2142    1498    1255    1201     910     822     548
                 532     445     397     345     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2224    5842    6176 
   Size:        274      80      33      51    1443     247    3618     334

Cuts at:   6176    6506    7187    7202    7280    8824    9217   11345   13057 
   Size:        330     681      15      78    1544     393    2128    1712

Cuts at:  13057      96 
   Size:        138

  Fragments arranged by size: 

                3618    2128    1712    1544    1443     681     393     334
                 330     274     247     138      80      78      51      33
                  15
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3175    3207    4530    4562    5603    5635    6413    6445    9037 
   Size:         32    1323      32    1041      32     778      32    2592

Cuts at:   9037    9069   13059   13091    3175 
   Size:         32    3990      32    3183

  Fragments arranged by size: 

                3990    3183    2592    1323    1041     778      32      32
                  32      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    9615    1316 
   Size:       8299    4800
 
XbaI T'CTAG_A

Cuts at:   8938   10435    8938 
   Size:       1497   11602
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2020    4465    4480    4751    2020 
   Size:       2445      15     271   10368

  Fragments arranged by size: 

               10368    2445     271      15
 
XhoI C'TCGA_G

Cuts at:   1943    2457    2478    2886    1943 
   Size:        514      21     408   12156

  Fragments arranged by size: 

               12156     514     408      21
 
XmaI C'CCGG_G

Cuts at:   1870   10424    1870 
   Size:       8554    4545
 
XmnI GAAnn'nnTTC

Cuts at:   9584    9852   11228    9584 
   Size:        268    1376   11455

  Fragments arranged by size: 

               11455    1376     268
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2010    2307   10907     276 
   Size:         53      83     186     936     476     297    8600    2468

  Fragments arranged by size: 

                8600    2468     936     476     297     186      83      53
            
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NsiI     NspI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
    PspXI     PsrI     PstI     PvuI    PvuII     SacI     SalI     SapI
     SbfI     ScaI     SfcI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     ApaI     AscI    AvrII    BsiWI   DraIII    EcoRV
     FseI    FspAI     NruI     PacI   PspOMI    RsrII    SacII    SanDI
    SexAI     SfiI     SrfI     SwaI
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