Restriction map for
pFN21AA0246
(Circular) (Six-base) MAPSORT of: pFN21AA0246.seq Check: 993 from: 1 to: 12415
pFN21AA0246 12415 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2071 2646 2915 3087 4509 4811 4901 6329 6461
Size: 575 269 172 1422 302 90 1428 132
Cuts at: 6461 6680 6752 7667 2071
Size: 219 72 915 6819
Fragments arranged by size:
6819 1428 1422 915 575 302 269 219
172 132 90 72
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7757 10225 278
Size: 53 83 186 936 6221 2468 2468
Fragments arranged by size:
6221 2468 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3268 3879 4080 5892 9758 1937
Size: 1331 611 201 1812 3866 4594
Fragments arranged by size:
4594 3866 1812 1331 611 201
Acc65I G'GTAC_C
Cuts at: 3729 9736 3729
Size: 6007 6408
AclI AA'CG_TT
Cuts at: 1563 10542 10915 1563
Size: 8979 373 3063
Fragments arranged by size:
8979 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2047 3544 4417 4942 5410 6781 7009 7207
Size: 415 1497 873 525 468 1371 228 198
Cuts at: 7207 7256 9438 10478 11862 12130 1632
Size: 49 2182 1040 1384 268 1917
Fragments arranged by size:
2182 1917 1497 1384 1371 1040 873 525
468 415 268 228 198 49
AflII C'TTAA_G
Cuts at: 820 1017 5027 820
Size: 197 4010 8208
Fragments arranged by size:
8208 4010 197
AflIII A'CryG_T
Cuts at: 1203 2165 4031 4418 5933 6701 11314 12291 1203
Size: 962 1866 387 1515 768 4613 977 1327
Fragments arranged by size:
4613 1866 1515 1327 977 962 768 387
AgeI A'CCGG_T
Cuts at: 2466 2985 11246 2466
Size: 519 8261 3635
Fragments arranged by size:
8261 3635 519
AhdI GACnn_n'nnGTC
Cuts at: 11144 11144
Size: 12415
AleI CACnn'nnGTG
Cuts at: 1166 3328 6146 1166
Size: 2162 2818 7435
Fragments arranged by size:
7435 2818 2162
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2519 2678 3944 8302 8618 1184
Size: 1335 159 1266 4358 316 4981
Fragments arranged by size:
4981 4358 1335 1266 316 159
AlwNI CAG_nnn'CTG
Cuts at: 2086 2608 3811 4134 5407 7594 7668 7791 7839
Size: 522 1203 323 1273 2187 74 123 48
Cuts at: 7839 7881 7990 8524 8749 9269 9282 10158 11730
Size: 42 109 534 225 520 13 876 1572
Cuts at: 11730 2086
Size: 2771
Fragments arranged by size:
2771 2187 1572 1273 1203 876 534 522
520 323 225 123 109 74 48 42
13
ApaI G_GGCC'C
Cuts at: 2016 3191 3624 4476 4784 5167 5645 6135 7448
Size: 1175 433 852 308 383 478 490 1313
Cuts at: 7448 8052 8401 9289 2016
Size: 604 349 888 5142
Fragments arranged by size:
5142 1313 1175 888 852 604 490 478
433 383 349 308
ApaLI G'TGCA_C
Cuts at: 2504 8196 8593 10472 11628 2504
Size: 5692 397 1879 1156 3291
Fragments arranged by size:
5692 3291 1879 1156 397
ApoI r'AATT_y
Cuts at: 1493 9724 9950 10102 12211 1493
Size: 8231 226 152 2109 1697
Fragments arranged by size:
8231 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 10969 160
Size: 872 9937 1606
Fragments arranged by size:
9937 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12415
AvaI C'yCGr_G
Cuts at: 1870 1943 2016 3854 4784 5111 5367 5916 6753
Size: 73 73 1838 930 327 256 549 837
Cuts at: 6753 7136 7174 8201 9507 9740 1870
Size: 383 38 1027 1306 233 4545
Fragments arranged by size:
4545 1838 1306 1027 930 837 549 383
327 256 233 73 73 38
AvrII C'CTAG_G
Cuts at: 6266 6266
Size: 12415
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2112 2145 2112
Size: 33 12382
BamHI G'GATC_C
Cuts at: 3514 3786 4251 9699 9745 10112 3514
Size: 272 465 5448 46 367 5817
Fragments arranged by size:
5817 5448 465 367 272 46
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2328 2658 3205 3729 4658
Size: 325 217 627 541 330 547 524 929
Cuts at: 4658 5077 5180 5416 5464 5568 5953 6430 7011
Size: 419 103 236 48 104 385 477 581
Cuts at: 7011 8039 8861 9385 9412 9736 11191 618
Size: 1028 822 524 27 324 1455 1842
Fragments arranged by size:
1842 1455 1028 929 822 627 581 547
541 524 524 477 419 385 330 325
324 236 217 104 103 48 27
BanII G_rGCy'C
Cuts at: 1391 2016 2193 2243 2701 2756 3191 3624 3998
Size: 625 177 50 458 55 435 433 374
Cuts at: 3998 4104 4476 4784 5111 5167 5645 5774 6135
Size: 106 372 308 327 56 478 129 361
Cuts at: 6135 6624 6771 6828 7324 7448 7512 8052 8111
Size: 489 147 57 496 124 64 540 59
Cuts at: 8111 8401 9093 9289 9493 9734 1391
Size: 290 692 196 204 241 4072
Fragments arranged by size:
4072 692 625 540 496 489 478 458
435 433 374 372 361 327 308 290
241 204 196 177 147 129 124 106
64 59 57 56 55 50
BbeI G_GCGC'C
Cuts at: 9416 9416
Size: 12415
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3454 4356 5653 928
Size: 971 1555 902 1297 7690
Fragments arranged by size:
7690 1555 1297 971 902
BbvCI CC'TCA_GC
Cuts at: 3131 5345 5799 8682 8727 9242 3131
Size: 2214 454 2883 45 515 6304
Fragments arranged by size:
6304 2883 2214 515 454 45
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2972 3006 3925 3959 4785 4819 4828
Size: 34 1035 34 919 34 826 34 9
Cuts at: 4828 4862 6266 6300 10606 10640 1903
Size: 34 1404 34 4306 34 3678
Fragments arranged by size:
4306 3678 1404 1035 919 826 34 34
34 34 34 34 34 9
BciVI GTATCCnnnnn_n'
Cuts at: 2555 6332 10307 11517 2555
Size: 3777 3975 1210 3453
Fragments arranged by size:
3975 3777 3453 1210
BclI T'GATC_A
Cuts at: 1548 2581 1548
Size: 1033 11382
BfrBI ATG'CAT
Cuts at: 3245 7462 3245
Size: 4217 8198
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2058 2346 2667
Size: 107 122 71 631 605 386 288 321
Cuts at: 2667 2916 4413 4480 4683 4863 4938 5386 6084
Size: 249 1497 67 203 180 75 448 698
Cuts at: 6084 6396 6462 7923 8106 8220 8496 8772 8937
Size: 312 66 1461 183 114 276 276 165
Cuts at: 8937 9293 9545 11026 136
Size: 356 252 1481 1525
Fragments arranged by size:
1525 1497 1481 1461 698 631 605 448
386 356 321 312 288 276 276 252
249 203 183 180 165 122 114 107
75 71 67 66
BglII A'GATC_T
Cuts at: 6868 7218 12410 6868
Size: 350 5192 6873
Fragments arranged by size:
6873 5192 350
BlpI GC'TnA_GC
Cuts at: 2437 5105 9832 2437
Size: 2668 4727 5020
Fragments arranged by size:
5020 4727 2668
Bme1580I G_kGCm'C
Cuts at: 1790 2016 2508 3191 3624 4418 4426 4476 4661
Size: 226 492 683 433 794 8 50 185
Cuts at: 4661 4784 4934 5082 5167 5185 5382 5469 5508
Size: 123 150 148 85 18 197 87 39
Cuts at: 5508 5645 6135 6163 6266 6754 7014 7448 8052
Size: 137 490 28 103 488 260 434 604
Cuts at: 8052 8200 8401 8597 8866 9289 9508 10476 11632
Size: 148 201 196 269 423 219 968 1156
Cuts at: 11632 1790
Size: 2573
Fragments arranged by size:
2573 1156 968 794 683 604 492 490
488 434 433 423 269 260 226 219
201 197 196 185 150 148 148 137
123 103 87 85 50 39 28 18
8
BmgBI CAC'GTC
Cuts at: 1318 2166 3883 4738 1318
Size: 848 1717 855 8995
Fragments arranged by size:
8995 1717 855 848
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2326 2623 2734
Size: 416 53 83 399 24 909 297 111
Cuts at: 2734 7159 8151 10146 11104 442
Size: 4425 992 1995 958 1753
Fragments arranged by size:
4425 1995 1753 992 958 909 416 399
297 111 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 7379 1056
Size: 6323 6092
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5611 5611
Size: 12415
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3214 3320 3602 4708 5009 5159 5543 5741
Size: 1975 106 282 1106 301 150 384 198
Cuts at: 5741 6785 7001 7616 7697 8186 8407 8546 8624
Size: 1044 216 615 81 489 221 139 78
Cuts at: 8624 9483 9692 11075 1239
Size: 859 209 1383 2579
Fragments arranged by size:
2579 1975 1383 1106 1044 859 615 489
384 301 282 221 216 209 198 150
139 106 81 78
Bpu10I CC'TnA_GC
Cuts at: 3131 5345 5799 6025 8682 8727 9242 3131
Size: 2214 454 226 2657 45 515 6304
Fragments arranged by size:
6304 2657 2214 515 454 226 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5758 6812 8476 10540 11405 11703 5758
Size: 1054 1664 2064 865 298 6470
Fragments arranged by size:
6470 2064 1664 1054 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2348 3707 4376 6574 7628 9065 9613
Size: 617 849 1359 669 2198 1054 1437 548
Cuts at: 9613 11078 882
Size: 1465 2219
Fragments arranged by size:
2219 2198 1465 1437 1359 1054 849 669
617 548
BsaAI yAC'GTr
Cuts at: 493 1204 2531 3469 4032 4419 4929 6943 8503
Size: 711 1327 938 563 387 510 2014 1560
Cuts at: 8503 8964 9096 10206 493
Size: 461 132 1110 2702
Fragments arranged by size:
2702 2014 1560 1327 1110 938 711 563
510 461 387 132
BsaBI GATnn'nnATC
Cuts at: 12409 12409
Size: 12415
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3459 7308 7754 9413
Size: 53 83 186 936 1926 3849 446 1659
Cuts at: 9413 10222 10604 11239 275
Size: 809 382 635 1451
Fragments arranged by size:
3849 1926 1659 1451 936 809 635 446
382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2466 2985 6143 6609 7330 10848 11246 11520 11667
Size: 519 3158 466 721 3518 398 274 147
Cuts at: 11667 12078 2466
Size: 411 2803
Fragments arranged by size:
3518 3158 2803 721 519 466 411 398
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2022 2052 3066 3096 4583
Size: 30 165 30 619 30 1014 30 1487
Cuts at: 4583 4613 5615 5645 6974 7004 7744 7774 1178
Size: 30 1002 30 1329 30 740 30 5819
Fragments arranged by size:
5819 1487 1329 1014 1002 740 619 165
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2015 3208 3217 3845 3986 5207 5210 7192
Size: 829 1193 9 628 141 1221 3 1982
Cuts at: 7192 8543 9689 1186
Size: 1351 1146 3912
Fragments arranged by size:
3912 1982 1351 1221 1193 1146 829 628
141 9 3
BseYI C'CCAG_C
Cuts at: 2233 2648 2855 3255 3411 3847 4488 4810 5209
Size: 415 207 400 156 436 641 322 399
Cuts at: 5209 5250 6118 6561 7802 7874 7928 8009 8742
Size: 41 868 443 1241 72 54 81 733
Cuts at: 8742 8749 8886 9051 11618 12114 2233
Size: 7 137 165 2567 496 2534
Fragments arranged by size:
2567 2534 1241 868 733 641 496 443
436 415 400 399 322 207 165 156
137 81 72 54 41 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2119 2312 2515 2887 3055 3113 4174
Size: 573 383 193 203 372 168 58 1061
Cuts at: 4174 4564 4654 4858 4909 5306 5812 5830 6124
Size: 390 90 204 51 397 506 18 294
Cuts at: 6124 6317 7091 7130 7562 7751 8056 8238 8257
Size: 193 774 39 432 189 305 182 19
Cuts at: 8257 8395 8588 1163
Size: 138 193 4990
Fragments arranged by size:
4990 1061 774 573 506 432 397 390
383 372 305 294 204 203 193 193
193 189 182 168 138 90 58 51
39 19 18
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2986 3676 5059 6309 9865 10626
Size: 275 186 985 690 1383 1250 3556 761
Cuts at: 10626 11654 1540
Size: 1028 2301
Fragments arranged by size:
3556 2301 1383 1250 1028 985 761 690
275 186
BsiHKAI G_wGCw'C
Cuts at: 2193 2508 2636 2747 6026 6624 6771 6828 7324
Size: 315 128 111 3279 598 147 57 496
Cuts at: 7324 7512 7618 8035 8200 8319 8445 8462 8597
Size: 188 106 417 165 119 126 17 135
Cuts at: 8597 9093 9469 9493 10476 10561 11632 2193
Size: 496 376 24 983 85 1071 2976
Fragments arranged by size:
3279 2976 1071 983 598 496 496 417
376 315 188 165 147 135 128 126
119 111 106 85 57 24 17
BsmI GAATG_Cn'
Cuts at: 2342 2696 3247 4636 5296 9926 10019 2342
Size: 354 551 1389 660 4630 93 4738
Fragments arranged by size:
4738 4630 1389 660 551 354 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3220 3220
Size: 12415
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2193 2243 2508 2636 2701 2747
Size: 399 226 177 50 265 128 65 46
Cuts at: 2747 2756 3191 3624 3998 4104 4418 4426 4476
Size: 9 435 433 374 106 314 8 50
Cuts at: 4476 4661 4784 4934 5082 5111 5167 5185 5382
Size: 185 123 150 148 29 56 18 197
Cuts at: 5382 5469 5508 5645 5774 6026 6135 6163 6266
Size: 87 39 137 129 252 109 28 103
Cuts at: 6266 6624 6754 6771 6828 7014 7324 7448 7512
Size: 358 130 17 57 186 310 124 64
Cuts at: 7512 7618 8035 8052 8111 8200 8319 8401 8445
Size: 106 417 17 59 89 119 82 44
Cuts at: 8445 8462 8597 8866 9093 9289 9469 9493 9508
Size: 17 135 269 227 196 180 24 15
Cuts at: 9508 9734 10476 10561 11632 1391
Size: 226 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 435 433 417 399 374
358 314 310 269 265 252 227 226
226 197 196 186 185 180 177 150
148 137 135 130 129 128 124 123
119 109 106 106 103 89 87 85
82 65 64 59 57 56 50 50
46 44 39 29 28 24 18 17
17 17 15 9 8
BspEI T'CCGG_A
Cuts at: 6609 7330 12078 6609
Size: 721 4748 6946
Fragments arranged by size:
6946 4748 721
BspHI T'CATG_A
Cuts at: 10304 12034 10304
Size: 1730 10685
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2071 2387 2646 2915 3087 4509 4811 4901
Size: 1227 316 259 269 172 1422 302 90
Cuts at: 4901 6329 6461 6556 6623 6680 6716 6752 6984
Size: 1428 132 95 67 57 36 36 232
Cuts at: 6984 7440 7667 8366 8610 9770 844
Size: 456 227 699 244 1160 3489
Fragments arranged by size:
3489 1428 1422 1227 1160 699 456 316
302 269 259 244 232 227 172 132
95 90 67 57 36 36
BsrBI CCG'CTC
Cuts at: 1394 1823 2259 5517 5736 10302 1394
Size: 429 436 3258 219 4566 3507
Fragments arranged by size:
4566 3507 3258 436 429 219
BsrDI GCAATG_nn'
Cuts at: 65 1248 2371 3652 4267 4346 4873 6355 7414
Size: 1183 1123 1281 615 79 527 1482 1059
Cuts at: 7414 8839 8974 10910 11084 65
Size: 1425 135 1936 174 1396
Fragments arranged by size:
1936 1482 1425 1396 1281 1183 1123 1059
615 527 174 135 79
BsrFI r'CCGG_y
Cuts at: 1700 1810 2466 2910 2985 4440 5036 7244 8462
Size: 110 656 444 75 1455 596 2208 1218
Cuts at: 8462 11059 11246 1700
Size: 2597 187 2869
Fragments arranged by size:
2869 2597 2208 1455 1218 656 596 444
187 110 75
BsrGI T'GTAC_A
Cuts at: 96 8270 96
Size: 8174 4241
BssHII G'CGCG_C
Cuts at: 1925 7064 1925
Size: 5139 7276
BssSI C'ACGA_G
Cuts at: 2140 3086 6080 10475 11487 2140
Size: 946 2994 4395 1012 3068
Fragments arranged by size:
4395 3068 2994 1012 946
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2142 2290 2691 3251 4302 4596 5395 7018
Size: 1348 148 401 560 1051 294 799 1623
Cuts at: 7018 7839 7905 8409 794
Size: 821 66 504 4800
Fragments arranged by size:
4800 1623 1348 1051 821 799 560 504
401 294 148 66
BstBI TT'CG_AA
Cuts at: 7453 11219 7453
Size: 3766 8649
BstEII G'GTnAC_C
Cuts at: 1184 3004 1184
Size: 1820 10595
BstXI CCAn_nnnn'nTGG
Cuts at: 2801 4262 4483 6392 6947 2801
Size: 1461 221 1909 555 8269
Fragments arranged by size:
8269 1909 1461 555 221
BstYI r'GATC_y
Cuts at: 1972 3514 3537 3786 4251 5946 6868 7218 9699
Size: 1542 23 249 465 1695 922 350 2481
Cuts at: 9699 9745 10112 10497 10514 11966 12052 12064 12410
Size: 46 367 385 17 1452 86 12 346
Cuts at: 12410 1972
Size: 1977
Fragments arranged by size:
2481 1977 1695 1542 1452 922 465 385
367 350 346 249 86 46 23 17
12
Bsu36I CC'TnA_GG
Cuts at: 2173 5685 6773 7692 7719 2173
Size: 3512 1088 919 27 6869
Fragments arranged by size:
6869 3512 1088 919 27
BtgI C'CryG_G
Cuts at: 513 1065 2004 2371 2431 2521 2794 3141 3745
Size: 552 939 367 60 90 273 347 604
Cuts at: 3745 3795 4366 4458 4612 4879 5455 5598 6031
Size: 50 571 92 154 267 576 143 433
Cuts at: 6031 6091 6163 6385 6391 6517 6726 6996 7948
Size: 60 72 222 6 126 209 270 952
Cuts at: 7948 8425 9519 9578 513
Size: 477 1094 59 3350
Fragments arranged by size:
3350 1094 952 939 604 576 571 552
477 433 367 347 273 270 267 222
209 154 143 126 92 90 72 60
60 59 50 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2814 3265 4282 6849 6902 8523
Size: 683 56 1588 451 1017 2567 53 1621
Cuts at: 8523 487
Size: 4379
Fragments arranged by size:
4379 2567 1621 1588 1017 683 451 56
53
BtsI GCAGTG_nn'
Cuts at: 2692 2729 2776 3961 4202 4249 4399 4492 4666
Size: 37 47 1185 241 47 150 93 174
Cuts at: 4666 4908 5484 6044 6073 7016 7906 7984 7991
Size: 242 576 560 29 943 890 78 7
Cuts at: 7991 8317 8525 8793 8997 9411 9467 9665 9931
Size: 326 208 268 204 414 56 198 266
Cuts at: 9931 10725 10745 2692
Size: 794 20 4362
Fragments arranged by size:
4362 1185 943 890 794 576 560 414
326 268 266 242 241 208 204 198
174 150 93 78 56 47 47 37
29 20 7
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 12415
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2827 2862 5251 5286 5656 5691 7832
Size: 35 2263 35 2389 35 370 35 2141
Cuts at: 7832 7867 529
Size: 35 5077
Fragments arranged by size:
5077 2389 2263 2141 370 35 35 35
35 35
DraI TTT'AAA
Cuts at: 9719 9719
Size: 12415
DraIII CAC_nnn'GTG
Cuts at: 2142 2512 2631 3091 4197 5575 5988 6727 8153
Size: 370 119 460 1106 1378 413 739 1426
Cuts at: 8153 8195 8806 2142
Size: 42 611 5751
Fragments arranged by size:
5751 1426 1378 1106 739 611 460 413
370 119 42
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5063 8520 11422 809
Size: 507 3747 3457 2902 1802
Fragments arranged by size:
3747 3457 2902 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2148 2368 2714
Size: 54 1425 50 275 18 318 220 346
Cuts at: 2714 2912 3144 3342 3673 3748 3888 4438 4699
Size: 198 232 198 331 75 140 550 261
Cuts at: 4699 4887 5226 5677 5731 6306 6388 6691 7242
Size: 188 339 451 54 575 82 303 551
Cuts at: 7242 8083 8260 8419 8781 9369 9483 9862 10751
Size: 841 177 159 362 588 114 379 889
Cuts at: 10751 12380 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 841 588 575 551 550
451 379 362 346 339 331 318 303
275 261 232 220 198 198 188 177
159 140 114 82 75 54 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3673 6306 9862 1537
Size: 275 1861 2633 3556 4090
Fragments arranged by size:
4090 3556 2633 1861 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3695 5192 5438 5876 9113 10345 1353
Size: 2342 1497 246 438 3237 1232 3423
Fragments arranged by size:
3423 3237 2342 1497 1232 438 246
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6794 8837 9563 11002 11376 11522
Size: 1064 138 5205 2043 726 1439 374 146
Cuts at: 11522 387
Size: 1280
Fragments arranged by size:
5205 2043 1439 1280 1064 726 374 146
138
EcoICRI GAG'CTC
Cuts at: 2191 6622 6769 6826 7322 7510 9091 9491 2191
Size: 4431 147 57 496 188 1581 400 5115
Fragments arranged by size:
5115 4431 1581 496 400 188 147 57
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2047 3214 3320 3544 3602 4417 4708
Size: 393 415 1167 106 224 58 815 291
Cuts at: 4708 4942 5009 5159 5410 5543 5741 6781 6785
Size: 234 67 150 251 133 198 1040 4
Cuts at: 6785 7001 7009 7207 7256 7616 7697 8186 8407
Size: 216 8 198 49 360 81 489 221
Cuts at: 8407 8546 8624 9438 9483 9692 10478 11075 11862
Size: 139 78 814 45 209 786 597 787
Cuts at: 11862 12130 1239
Size: 268 1524
Fragments arranged by size:
1524 1167 1040 815 814 787 786 597
489 415 393 360 291 268 251 234
224 221 216 209 198 198 150 139
133 106 81 78 67 58 49 45
8 4
EcoNI CCTnn'n_nnAGG
Cuts at: 5189 5348 5585 5612 6887 7235 8288 5189
Size: 159 237 27 1275 348 1053 9316
Fragments arranged by size:
9316 1275 1053 348 237 159 27
EcoO109I rG'GnC_Cy
Cuts at: 2012 2013 2020 2376 2406 2824 3188 3312 3493
Size: 1 7 356 30 418 364 124 181
Cuts at: 3493 3504 3621 3808 3851 3862 4266 4650 4781
Size: 11 117 187 43 11 404 384 131
Cuts at: 4781 5163 5590 5642 5711 5725 5758 5803 6226
Size: 382 427 52 69 14 33 45 423
Cuts at: 6226 6973 7281 7467 7818 8049 8098 9266 9286
Size: 747 308 186 351 231 49 1168 20
Cuts at: 9286 2012
Size: 5141
Fragments arranged by size:
5141 1168 747 427 423 418 404 384
382 364 356 351 308 231 187 186
181 131 124 117 69 52 49 45
43 33 30 20 14 11 11 7
1
EcoRI G'AATT_C
Cuts at: 9724 10102 9724
Size: 378 12037
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4831 9124 4831
Size: 4293 8122
FseI GG_CCGG'CC
Cuts at: 7248 7248
Size: 12415
FspI TGC'GCA
Cuts at: 6915 10921 6915
Size: 4006 8409
FspAI rTGC'GCAy
Cuts at: 6915 6915
Size: 12415
HaeII r_GCGC'y
Cuts at: 1678 2352 7145 7735 8482 9416 11562 1678
Size: 674 4793 590 747 934 2146 2531
Fragments arranged by size:
4793 2531 2146 934 747 674 590
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2471 2503 2797 2829 3066
Size: 32 135 32 899 32 294 32 237
Cuts at: 3066 3098 3261 3293 3525 3557 4559 4591 6620
Size: 32 163 32 232 32 1002 32 2029
Cuts at: 6620 6652 7744 7776 9557 9589 11056 11088 11130
Size: 32 1092 32 1781 32 1467 32 42
Cuts at: 11130 11162 1373
Size: 32 2626
Fragments arranged by size:
2626 2029 1781 1467 1092 1002 899 294
237 232 163 135 42 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 9759 10005 669
Size: 988 281 7821 246 3079
Fragments arranged by size:
7821 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 7911 748
Size: 7163 5252
HpaI GTT'AAC
Cuts at: 10005 10005
Size: 12415
KasI G'GCGC_C
Cuts at: 9412 9412
Size: 12415
KpnI G_GTAC'C
Cuts at: 3733 9740 3733
Size: 6007 6408
MfeI C'AATT_G
Cuts at: 4237 7849 10014 4237
Size: 3612 2165 6638
Fragments arranged by size:
6638 3612 2165
MluI A'CGCG_T
Cuts at: 12291 12291
Size: 12415
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3764 4239 8514 9204 11529 11713 1305
Size: 2459 475 4275 690 2325 184 2007
Fragments arranged by size:
4275 2459 2325 2007 690 475 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2370 2716 3146 4889 5228
Size: 54 1425 343 538 346 430 1743 339
Cuts at: 5228 5679 6693 8421 8783 9371 10
Size: 451 1014 1728 362 588 3054
Fragments arranged by size:
3054 1743 1728 1425 1014 588 538 451
430 362 346 343 339 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2799 3328 4503 4758 5501 6146 6390
Size: 648 1633 529 1175 255 743 645 244
Cuts at: 6390 6854 8583 10373 10732 10891 518
Size: 464 1729 1790 359 159 2042
Fragments arranged by size:
2042 1790 1729 1633 1175 743 648 645
529 464 359 255 244 159
MspA1I CmG'CkG
Cuts at: 1606 2233 2433 2499 2652 3011 3027 3130 3185
Size: 627 200 66 153 359 16 103 55
Cuts at: 3185 3698 3754 3868 4512 4918 4962 5104 5407
Size: 513 56 114 644 406 44 142 303
Cuts at: 5407 5844 5863 6063 6234 6442 6504 6579 6597
Size: 437 19 200 171 208 62 75 18
Cuts at: 6597 6925 7227 7546 7591 7765 7878 8070 8088
Size: 328 302 319 45 174 113 192 18
Cuts at: 8088 8229 8427 8553 8652 8749 9055 9461 9831
Size: 141 198 126 99 97 306 406 370
Cuts at: 9831 10508 11656 11901 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 644 627 513 437 406
406 370 359 328 319 306 303 302
245 208 200 200 198 192 174 171
153 142 141 126 114 113 103 99
97 75 66 62 56 55 45 44
19 18 18 16
NaeI GCC'GGC
Cuts at: 2912 5038 7246 2912
Size: 2126 2208 8081
Fragments arranged by size:
8081 2208 2126
NarI GG'CG_CC
Cuts at: 9413 9413
Size: 12415
NcoI C'CATG_G
Cuts at: 513 1065 2004 2521 2794 3795 4366 4458 5455
Size: 552 939 517 273 1001 571 92 997
Cuts at: 5455 5598 6031 6091 6163 6391 9519 513
Size: 143 433 60 72 228 3128 3409
Fragments arranged by size:
3409 3128 1001 997 939 571 552 517
433 273 228 143 92 72 60
NdeI CA'TA_TG
Cuts at: 387 6664 387
Size: 6277 6138
NgoMIV G'CCGG_C
Cuts at: 2910 5036 7244 2910
Size: 2126 2208 8081
Fragments arranged by size:
8081 2208 2126
NheI G'CTAG_C
Cuts at: 1052 7375 1052
Size: 6323 6092
NotI GC'GGCC_GC
Cuts at: 9862 9862
Size: 12415
NsiI A_TGCA'T
Cuts at: 3247 7464 3247
Size: 4217 8198
NspI r_CATG'y
Cuts at: 1132 1229 3913 4508 4677 5937 6450 6705 6788
Size: 97 2684 595 169 1260 513 255 83
Cuts at: 6788 8694 9176 9773 11318 1132
Size: 1906 482 597 1545 2229
Fragments arranged by size:
2684 2229 1906 1545 1260 597 595 513
482 255 169 97 83
PasI CC'CwG_GG
Cuts at: 1348 2342 3509 4646 4654 5530 6002 7201 9299
Size: 994 1167 1137 8 876 472 1199 2098
Cuts at: 9299 1348
Size: 4464
Fragments arranged by size:
4464 2098 1199 1167 1137 994 876 472
8
PciI A'CATG_T
Cuts at: 5933 6701 11314 5933
Size: 768 4613 7034
Fragments arranged by size:
7034 4613 768
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2366 3811 3913 4394 4885 5250 5461 6727
Size: 1258 1445 102 481 491 365 211 1266
Cuts at: 6727 7594 7809 7828 7866 8749 8806 9367 1108
Size: 867 215 19 38 883 57 561 4156
Fragments arranged by size:
4156 1445 1266 1258 883 867 561 491
481 365 215 211 102 57 38 19
PfoI T'CCnGG_A
Cuts at: 2193 3230 3297 3579 3606 4980 5436 6624 6810
Size: 1037 67 282 27 1374 456 1188 186
Cuts at: 6810 8610 9069 9078 2193
Size: 1800 459 9 5530
Fragments arranged by size:
5530 1800 1374 1188 1037 459 456 282
186 67 27 9
PmeI GTTT'AAAC
Cuts at: 9719 9719
Size: 12415
PmlI CAC'GTG
Cuts at: 2531 3469 4032 4419 4929 6943 8964 2531
Size: 938 563 387 510 2014 2021 5982
Fragments arranged by size:
5982 2021 2014 938 563 510 387
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 10484 10516 11997 12029 10484
Size: 32 1481 32 10870
Fragments arranged by size:
10870 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2376 2406 2824 3312 3504 3808 3851 3862 4650
Size: 30 418 488 192 304 43 11 788
Cuts at: 4650 5758 6973 7467 2376
Size: 1108 1215 494 7324
Fragments arranged by size:
7324 1215 1108 788 494 488 418 304
192 43 30 11
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 12415
PsiI TTA'TAA
Cuts at: 9985 9985
Size: 12415
PspOMI G'GGCC_C
Cuts at: 2012 3187 3620 4472 4780 5163 5641 6131 7444
Size: 1175 433 852 308 383 478 490 1313
Cuts at: 7444 8048 8397 9285 2012
Size: 604 349 888 5142
Fragments arranged by size:
5142 1313 1175 888 852 604 490 478
433 383 349 308
PstI C_TGCA'G
Cuts at: 830 2267 2657 3129 3717 3873 3968 4772 5095
Size: 1437 390 472 588 156 95 804 323
Cuts at: 5095 5136 5403 5451 5868 6578 7490 7664 8175
Size: 41 267 48 417 710 912 174 511
Cuts at: 8175 8312 8438 8520 9767 830
Size: 137 126 82 1247 3478
Fragments arranged by size:
3478 1437 1247 912 804 710 588 511
472 417 390 323 267 174 156 137
126 95 82 48 41
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 12415
PvuII CAG'CTG
Cuts at: 2233 2499 2652 3027 3130 3754 4962 5104 5407
Size: 266 153 375 103 624 1208 142 303
Cuts at: 5407 5863 6504 6579 6925 7591 7878 8229 8749
Size: 456 641 75 346 666 287 351 520
Cuts at: 8749 2233
Size: 5899
Fragments arranged by size:
5899 1208 666 641 624 520 456 375
351 346 303 287 266 153 142 103
75
RsrII CG'GwC_CG
Cuts at: 4609 4609
Size: 12415
SacI G_AGCT'C
Cuts at: 2193 6624 6771 6828 7324 7512 9093 9493 2193
Size: 4431 147 57 496 188 1581 400 5115
Fragments arranged by size:
5115 4431 1581 496 400 188 147 57
SacII CC_GC'GG
Cuts at: 2434 8428 2434
Size: 5994 6421
SalI G'TCGA_C
Cuts at: 1936 9757 1936
Size: 7821 4594
SanDI GG'GwC_CC
Cuts at: 3504 3851 3862 4650 7467 3504
Size: 347 11 788 2817 8452
Fragments arranged by size:
8452 2817 788 347 11
SapI GCTCTTCn'nnn_
Cuts at: 5876 5876
Size: 12415
SbfI CC_TGCA'GG
Cuts at: 9767 9767
Size: 12415
ScaI AGT'ACT
Cuts at: 10663 12375 10663
Size: 1712 10703
SexAI A'CCwGG_T
Cuts at: 3804 5154 3804
Size: 1350 11065
SfcI C'TryA_G
Cuts at: 826 1045 2263 2653 3125 3265 3713 3869 3964
Size: 219 1218 390 472 140 448 156 95
Cuts at: 3964 4768 5091 5132 5399 5447 5864 6574 7486
Size: 804 323 41 267 48 417 710 912
Cuts at: 7486 7660 8171 8308 8326 8434 8516 9763 10898
Size: 174 511 137 18 108 82 1247 1135
Cuts at: 10898 11579 11770 826
Size: 681 191 1471
Fragments arranged by size:
1471 1247 1218 1135 912 804 710 681
511 472 448 417 390 323 267 219
191 174 156 140 137 108 95 82
48 41 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 9293 9293
Size: 12415
SfoI GGC'GCC
Cuts at: 9414 9414
Size: 12415
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12415
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 12415
SmaI CCC'GGG
Cuts at: 1872 2018 4786 5918 9742 1872
Size: 146 2768 1132 3824 4545
Fragments arranged by size:
4545 3824 2768 1132 146
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5027 5773 6827 8455 10519 11420
Size: 197 926 3084 746 1054 1628 2064 901
Cuts at: 11420 11682 820
Size: 262 1553
Fragments arranged by size:
3084 2064 1628 1553 1054 926 901 746
262 197
SnaBI TAC'GTA
Cuts at: 493 8503 10206 493
Size: 8010 1703 2702
Fragments arranged by size:
8010 2702 1703
SpeI A'CTAG_T
Cuts at: 152 152
Size: 12415
SphI G_CATG'C
Cuts at: 1132 4508 6788 8694 9176 9773 1132
Size: 3376 2280 1906 482 597 3774
Fragments arranged by size:
3774 3376 2280 1906 597 482
SspI AAT'ATT
Cuts at: 5 52 10339 5
Size: 47 10287 2081
Fragments arranged by size:
10287 2081 47
StuI AGG'CCT
Cuts at: 1517 2300 2897 2919 3557 7343 7418 7724 7827
Size: 783 597 22 638 3786 75 306 103
Cuts at: 7827 9240 1517
Size: 1413 4692
Fragments arranged by size:
4692 3786 1413 783 638 597 306 103
75 22
StyI C'CwwG_G
Cuts at: 513 1065 2004 2521 2757 2794 2820 2842 3777
Size: 552 939 517 236 37 26 22 935
Cuts at: 3777 3795 4068 4366 4458 5138 5455 5598 6031
Size: 18 273 298 92 680 317 143 433
Cuts at: 6031 6091 6163 6266 6391 6423 7419 7609 7822
Size: 60 72 103 125 32 996 190 213
Cuts at: 7822 8101 9519 9854 513
Size: 279 1418 335 3074
Fragments arranged by size:
3074 1418 996 939 935 680 552 517
433 335 317 298 279 273 236 213
190 143 125 103 92 72 60 37
32 26 22 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2642 4676 5133 5201 5272 5890 7029
Size: 1255 1062 2034 457 68 71 618 1139
Cuts at: 7029 7508 8849 8936 9065 9707 10442 10459 10612
Size: 479 1341 87 129 642 735 17 153
Cuts at: 10612 10797 11242 325
Size: 185 445 1498
Fragments arranged by size:
2034 1498 1341 1255 1139 1062 735 642
618 479 457 445 185 153 129 87
71 68 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2227 3396 8270
Size: 274 80 33 51 1443 250 1169 4874
Cuts at: 8270 10661 12373 96
Size: 2391 1712 138
Fragments arranged by size:
4874 2391 1712 1443 1169 274 250 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2022 2054 4183 4215 5615 5647 5872 5904 8005
Size: 32 2129 32 1400 32 225 32 2101
Cuts at: 8005 8037 9096 9128 12375 12407 2022
Size: 32 1059 32 3247 32 2030
Fragments arranged by size:
3247 2129 2101 2030 1400 1059 225 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 7121 1316
Size: 5805 6610
XbaI T'CTAG_A
Cuts at: 9751 9751
Size: 12415
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2339 2528 3473 4610 5302 5518 7916 8297 9379
Size: 189 945 1137 692 216 2398 381 1082
Cuts at: 9379 9601 2339
Size: 222 5153
Fragments arranged by size:
5153 2398 1137 1082 945 692 381 222
216 189
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 12415
XmaI C'CCGG_G
Cuts at: 1870 2016 4784 5916 9740 1870
Size: 146 2768 1132 3824 4545
Fragments arranged by size:
4545 3824 2768 1132 146
XmnI GAAnn'nnTTC
Cuts at: 10544 10544
Size: 12415
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7755 10223 276
Size: 53 83 186 936 6221 2468 2468
Fragments arranged by size:
6221 2468 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BsiWI BstZ17I EcoRV NruI PacI
PspXI PsrI SrfI SwaI