(Circular) (Six-base) MAPSORT of: pFN21AE2505.seq Check: 4952 from: 1 to: 7825 pFN21AE2505 7825 bp With 182 enzymes: SgfI * February 16, 2012 14:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2043 2161 2485 3473 3958 2043 Size: 118 324 988 485 5910 Fragments arranged by size: 5910 988 485 324 118 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5635 278 Size: 53 83 186 936 4099 2468 Fragments arranged by size: 4099 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2328 4320 5168 1937 Size: 391 1992 848 4594 Fragments arranged by size: 4594 1992 848 391 Acc65I G'GTAC_C Cuts at: 2234 3209 5146 2234 Size: 975 1937 4913 Fragments arranged by size: 4913 1937 975 AclI AA'CG_TT Cuts at: 1563 5952 6325 1563 Size: 4389 373 3063 Fragments arranged by size: 4389 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2055 2650 2718 3244 4947 5888 7272 7540 Size: 423 595 68 526 1703 941 1384 268 Cuts at: 7540 1632 Size: 1917 Fragments arranged by size: 1917 1703 1384 941 595 526 423 268 68 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7628 AflIII A'CryG_T Cuts at: 1203 3759 6724 7701 1203 Size: 2556 2965 977 1327 Fragments arranged by size: 2965 2556 1327 977 AgeI A'CCGG_T Cuts at: 6656 6656 Size: 7825 AhdI GACnn_n'nnGTC Cuts at: 3790 6554 3790 Size: 2764 5061 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7825 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2987 1184 Size: 1803 6022 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3131 3163 4034 4066 3131 Size: 32 871 32 6890 Fragments arranged by size: 6890 871 32 32 AlwNI CAG_nnn'CTG Cuts at: 2338 3017 4021 4925 5568 7140 2338 Size: 679 1004 904 643 1572 3023 Fragments arranged by size: 3023 1572 1004 904 679 643 ApaI G_GGCC'C Cuts at: 4101 4398 4971 4101 Size: 297 573 6955 Fragments arranged by size: 6955 573 297 ApaLI G'TGCA_C Cuts at: 5882 7038 5882 Size: 1156 6669 ApoI r'AATT_y Cuts at: 1493 2405 4953 5134 5360 5512 7621 1493 Size: 912 2548 181 226 152 2109 1697 Fragments arranged by size: 2548 2109 1697 912 226 181 152 AseI AT'TA_AT Cuts at: 160 1032 6379 160 Size: 872 5347 1606 Fragments arranged by size: 5347 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7825 AvaI C'yCGr_G Cuts at: 1870 1943 2193 3172 3330 4287 4397 4623 5150 Size: 73 250 979 158 957 110 226 527 Cuts at: 5150 1870 Size: 4545 Fragments arranged by size: 4545 979 957 527 250 226 158 110 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4199 4232 4199 Size: 33 7792 BamHI G'GATC_C Cuts at: 2093 4983 5155 5522 2093 Size: 2890 172 367 4396 Fragments arranged by size: 4396 2890 367 172 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2234 2357 2909 3209 3416 Size: 325 217 627 447 123 552 300 207 Cuts at: 3416 4141 4291 4298 5069 5146 6601 618 Size: 725 150 7 771 77 1455 1842 Fragments arranged by size: 1842 1455 771 725 627 552 447 325 300 217 207 150 123 77 7 BanII G_rGCy'C Cuts at: 1391 2871 4101 4174 4185 4398 4440 4971 5124 Size: 1480 1230 73 11 213 42 531 153 Cuts at: 5124 5144 1391 Size: 20 4072 Fragments arranged by size: 4072 1480 1230 531 213 153 73 42 20 11 BbeI G_GCGC'C Cuts at: 4302 4302 Size: 7825 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2130 2656 3174 4935 928 Size: 971 231 526 518 1761 3818 Fragments arranged by size: 3818 1761 971 526 518 231 BbvCI CC'TCA_GC Cuts at: 4428 4428 Size: 7825 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3983 4017 4116 4150 6016 6050 1903 Size: 34 2046 34 99 34 1866 34 3678 Fragments arranged by size: 3678 2046 1866 99 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4541 5717 6927 4541 Size: 1176 1210 5439 Fragments arranged by size: 5439 1210 1176 BclI T'GATC_A Cuts at: 1548 3423 1548 Size: 1875 5950 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2989 3676 4096 Size: 107 122 71 631 605 1317 687 420 Cuts at: 4096 6436 136 Size: 2340 1525 Fragments arranged by size: 2340 1525 1317 687 631 605 420 122 107 71 BglII A'GATC_T Cuts at: 7820 7820 Size: 7825 BlpI GC'TnA_GC Cuts at: 2679 2865 2979 3158 3881 5242 2679 Size: 186 114 179 723 1361 5262 Fragments arranged by size: 5262 1361 723 186 179 114 Bme1580I G_kGCm'C Cuts at: 1790 2360 2912 3681 4082 4101 4398 4624 4971 Size: 570 552 769 401 19 297 226 347 Cuts at: 4971 5886 7042 1790 Size: 915 1156 2573 Fragments arranged by size: 2573 1156 915 769 570 552 401 347 297 226 19 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7825 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2345 2423 4092 Size: 416 53 83 399 24 928 78 1669 Cuts at: 4092 4303 4701 4913 5556 6514 442 Size: 211 398 212 643 958 1753 Fragments arranged by size: 1753 1669 958 928 643 416 399 398 212 211 83 78 53 24 BmtI G_CTAG'C Cuts at: 1056 3236 1056 Size: 2180 5645 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2190 2270 2801 3379 3393 3693 3907 4087 Size: 951 80 531 578 14 300 214 180 Cuts at: 4087 5138 6485 1239 Size: 1051 1347 2579 Fragments arranged by size: 2579 1347 1051 951 578 531 300 214 180 80 14 Bpu10I CC'TnA_GC Cuts at: 4428 4428 Size: 7825 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3535 4759 5950 6815 7113 3535 Size: 1224 1191 865 298 4247 Fragments arranged by size: 4247 1224 1191 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2418 2741 3698 4480 6488 882 Size: 617 919 323 957 782 2008 2219 Fragments arranged by size: 2219 2008 957 919 782 617 323 BsaAI yAC'GTr Cuts at: 493 1204 3762 5616 493 Size: 711 2558 1854 2702 Fragments arranged by size: 2702 2558 1854 711 BsaBI GATnn'nnATC Cuts at: 3314 4913 5099 7819 3314 Size: 1599 186 2720 3320 Fragments arranged by size: 3320 2720 1599 186 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2548 3280 4199 4299 Size: 53 83 186 936 1015 732 919 100 Cuts at: 4299 5632 6014 6649 275 Size: 1333 382 635 1451 Fragments arranged by size: 1451 1333 1015 936 919 732 635 382 186 100 83 53 BsaWI w'CCGG_w Cuts at: 3684 4113 6258 6656 6930 7077 7488 3684 Size: 429 2145 398 274 147 411 4021 Fragments arranged by size: 4021 2145 429 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2159 2189 3011 3041 3391 Size: 30 165 30 756 30 822 30 350 Cuts at: 3391 3421 3831 3861 1178 Size: 30 410 30 5142 Fragments arranged by size: 5142 822 756 410 350 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2056 2168 2180 2432 2501 4176 1186 Size: 870 112 12 252 69 1675 4835 Fragments arranged by size: 4835 1675 870 252 112 69 12 BseYI C'CCAG_C Cuts at: 2448 2720 2740 2885 3134 3735 4021 4565 5053 Size: 272 20 145 249 601 286 544 488 Cuts at: 5053 7028 7524 2448 Size: 1975 496 2749 Fragments arranged by size: 2749 1975 601 544 496 488 286 272 249 145 20 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2975 2993 3069 3996 4395 4532 1163 Size: 573 1239 18 76 927 399 137 4456 Fragments arranged by size: 4456 1239 927 573 399 137 76 18 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5275 6036 7064 1540 Size: 275 186 3274 761 1028 2301 Fragments arranged by size: 3274 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3357 4185 5124 5886 5971 7042 3357 Size: 828 939 762 85 1071 4140 Fragments arranged by size: 4140 1071 939 828 762 85 BsmI GAATG_Cn' Cuts at: 5336 5429 5336 Size: 93 7732 BsmBI CGTCTCn'nnnn_ Cuts at: 4221 4221 Size: 7825 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2360 2871 2912 3357 3681 4082 4101 Size: 399 570 511 41 445 324 401 19 Cuts at: 4101 4174 4185 4398 4440 4624 4971 5124 5144 Size: 73 11 213 42 184 347 153 20 Cuts at: 5144 5886 5971 7042 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 570 511 445 401 399 347 324 213 184 153 85 73 42 41 20 19 11 BspEI T'CCGG_A Cuts at: 4113 7488 4113 Size: 3375 4450 BspHI T'CATG_A Cuts at: 2295 2440 5714 7444 2295 Size: 145 3274 1730 2676 Fragments arranged by size: 3274 2676 1730 145 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2043 2161 2485 2761 3042 3150 3473 3958 Size: 1199 118 324 276 281 108 323 485 Cuts at: 3958 4251 5180 844 Size: 293 929 3489 Fragments arranged by size: 3489 1199 929 485 324 323 293 281 276 118 108 BsrBI CCG'CTC Cuts at: 1394 1823 5712 1394 Size: 429 3889 3507 Fragments arranged by size: 3889 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2583 4006 6320 6494 65 Size: 1183 1335 1423 2314 174 1396 Fragments arranged by size: 2314 1423 1396 1335 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3056 3441 4838 4895 6469 6656 1700 Size: 110 1246 385 1397 57 1574 187 2869 Fragments arranged by size: 2869 1574 1397 1246 385 187 110 57 BsrGI T'GTAC_A Cuts at: 96 2580 96 Size: 2484 5341 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7825 BssSI C'ACGA_G Cuts at: 4415 5885 6897 4415 Size: 1470 1012 5343 Fragments arranged by size: 5343 1470 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2176 2318 794 Size: 1382 142 6301 Fragments arranged by size: 6301 1382 142 BstBI TT'CG_AA Cuts at: 6629 6629 Size: 7825 BstEII G'GTnAC_C Cuts at: 1184 2773 1184 Size: 1589 6236 BstXI CCAn_nnnn'nTGG Cuts at: 2353 2641 3811 2353 Size: 288 1170 6367 Fragments arranged by size: 6367 1170 288 BstYI r'GATC_y Cuts at: 1972 2093 2880 3309 3715 4365 4548 4983 5155 Size: 121 787 429 406 650 183 435 172 Cuts at: 5155 5522 5907 5924 7376 7462 7474 7820 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 787 650 435 429 406 385 367 346 183 172 121 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4147 4147 Size: 7825 BtgI C'CryG_G Cuts at: 513 1065 2004 2160 3291 3298 3348 3615 3745 Size: 552 939 156 1131 7 50 267 130 Cuts at: 3745 3892 4384 4739 5088 513 Size: 147 492 355 349 3250 Fragments arranged by size: 3250 1131 939 552 492 355 349 267 156 147 130 50 7 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3901 5108 487 Size: 683 56 2675 1207 3204 Fragments arranged by size: 3204 2675 1207 683 56 BtsI GCAGTG_nn' Cuts at: 2858 4407 5341 6135 6155 2858 Size: 1549 934 794 20 4528 Fragments arranged by size: 4528 1549 934 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7825 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3330 3365 529 Size: 35 2766 35 4989 Fragments arranged by size: 4989 2766 35 35 DraI TTT'AAA Cuts at: 5129 5129 Size: 7825 DraIII CAC_nnn'GTG Cuts at: 2557 5005 5089 2557 Size: 2448 84 5293 Fragments arranged by size: 5293 2448 84 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4204 4808 6832 809 Size: 507 2888 604 2024 1802 Fragments arranged by size: 2888 2024 1802 604 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2628 4936 5272 Size: 54 1425 50 275 18 798 2308 336 Cuts at: 5272 6161 7790 8 Size: 889 1629 43 Fragments arranged by size: 2308 1629 1425 889 798 336 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5272 1537 Size: 275 3460 4090 Fragments arranged by size: 4090 3460 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2074 4428 5755 1353 Size: 721 2354 1327 3423 Fragments arranged by size: 3423 2354 1327 721 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2942 3813 5039 6412 6786 6932 Size: 1064 138 1353 871 1226 1373 374 146 Cuts at: 6932 387 Size: 1280 Fragments arranged by size: 1373 1353 1280 1226 1064 871 374 146 138 EcoICRI GAG'CTC Cuts at: 4183 5122 4183 Size: 939 6886 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2055 2190 2270 2650 2718 2801 3244 Size: 393 423 135 80 380 68 83 443 Cuts at: 3244 3379 3393 3693 3907 4087 4947 5138 5888 Size: 135 14 300 214 180 860 191 750 Cuts at: 5888 6485 7272 7540 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 860 787 750 597 443 423 393 380 300 268 214 191 180 135 135 83 80 68 14 EcoNI CCTnn'n_nnAGG Cuts at: 4255 4255 Size: 7825 EcoO109I rG'GnC_Cy Cuts at: 3139 3830 4098 4671 4801 4967 3139 Size: 691 268 573 130 166 5997 Fragments arranged by size: 5997 691 573 268 166 130 EcoRI G'AATT_C Cuts at: 2405 5134 5512 2405 Size: 2729 378 4718 Fragments arranged by size: 4718 2729 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2680 2680 Size: 7825 FspI TGC'GCA Cuts at: 6331 6331 Size: 7825 HaeII r_GCGC'y Cuts at: 1678 3228 4302 6972 1678 Size: 1550 1074 2670 2531 Fragments arranged by size: 2670 2531 1550 1074 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3011 3043 3391 3423 3776 Size: 32 135 32 1439 32 348 32 353 Cuts at: 3776 3808 4170 4202 6466 6498 6540 6572 1373 Size: 32 362 32 2264 32 42 32 2626 Fragments arranged by size: 2626 2264 1439 362 353 348 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3385 4192 5169 5415 669 Size: 988 281 1447 807 977 246 3079 Fragments arranged by size: 3079 1447 988 977 807 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7825 HpaI GTT'AAC Cuts at: 5415 5415 Size: 7825 KasI G'GCGC_C Cuts at: 4298 4298 Size: 7825 KpnI G_GTAC'C Cuts at: 2238 3213 5150 2238 Size: 975 1937 4913 Fragments arranged by size: 4913 1937 975 MfeI C'AATT_G Cuts at: 5424 5424 Size: 7825 MluI A'CGCG_T Cuts at: 7701 7701 Size: 7825 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2370 3054 6939 7123 1305 Size: 1065 684 3885 184 2007 Fragments arranged by size: 3885 2007 1065 684 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2630 4938 10 Size: 54 1425 343 798 2308 2897 Fragments arranged by size: 2897 2308 1425 798 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2969 3764 4724 5024 5030 5783 6142 Size: 648 1803 795 960 300 6 753 359 Cuts at: 6142 6301 518 Size: 159 2042 Fragments arranged by size: 2042 1803 960 795 753 648 359 300 159 6 MspA1I CmG'CkG Cuts at: 1606 2272 2505 2864 3293 3300 3739 3885 4158 Size: 666 233 359 429 7 439 146 273 Cuts at: 4158 4386 4427 4583 4632 4741 5241 5918 7066 Size: 228 41 156 49 109 500 677 1148 Cuts at: 7066 7311 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 666 500 439 429 359 273 245 233 228 156 146 109 49 41 7 NaeI GCC'GGC Cuts at: 3058 3058 Size: 7825 NarI GG'CG_CC Cuts at: 4299 4299 Size: 7825 NcoI C'CATG_G Cuts at: 513 1065 2004 3615 3745 513 Size: 552 939 1611 130 4593 Fragments arranged by size: 4593 1611 939 552 130 NdeI CA'TA_TG Cuts at: 387 387 Size: 7825 NgoMIV G'CCGG_C Cuts at: 3056 3056 Size: 7825 NheI G'CTAG_C Cuts at: 1052 3232 1052 Size: 2180 5645 NotI GC'GGCC_GC Cuts at: 5272 5272 Size: 7825 NspI r_CATG'y Cuts at: 1132 1229 2187 3181 5183 6728 1132 Size: 97 958 994 2002 1545 2229 Fragments arranged by size: 2229 2002 1545 994 958 97 PasI CC'CwG_GG Cuts at: 1348 2089 4440 1348 Size: 741 2351 4733 Fragments arranged by size: 4733 2351 741 PciI A'CATG_T Cuts at: 6724 6724 Size: 7825 PflMI CCAn_nnn'nTGG Cuts at: 1108 2275 2338 3190 3778 4461 4945 5066 5089 Size: 1167 63 852 588 683 484 121 23 Cuts at: 5089 1108 Size: 3844 Fragments arranged by size: 3844 1167 852 683 588 484 121 63 23 PfoI T'CCnGG_A Cuts at: 4763 4763 Size: 7825 PmeI GTTT'AAAC Cuts at: 5129 5129 Size: 7825 PmlI CAC'GTG Cuts at: 3762 3762 Size: 7825 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5894 5926 7407 7439 5894 Size: 32 1481 32 6280 Fragments arranged by size: 6280 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3139 3830 4801 3139 Size: 691 971 6163 Fragments arranged by size: 6163 971 691 PshAI GACnn'nnGTC Cuts at: 1613 2547 1613 Size: 934 6891 PsiI TTA'TAA Cuts at: 5395 5395 Size: 7825 PspOMI G'GGCC_C Cuts at: 4097 4394 4967 4097 Size: 297 573 6955 Fragments arranged by size: 6955 573 297 PstI C_TGCA'G Cuts at: 830 2403 2880 3955 4834 4993 5177 830 Size: 1573 477 1075 879 159 184 3478 Fragments arranged by size: 3478 1573 1075 879 477 184 159 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7825 PvuII CAG'CTG Cuts at: 2272 2864 3739 3885 4427 4583 2272 Size: 592 875 146 542 156 5514 Fragments arranged by size: 5514 875 592 542 156 146 RsrII CG'GwC_CG Cuts at: 4588 4588 Size: 7825 SacI G_AGCT'C Cuts at: 4185 5124 4185 Size: 939 6886 SacII CC_GC'GG Cuts at: 3294 3301 4387 4742 3294 Size: 7 1086 355 6377 Fragments arranged by size: 6377 1086 355 7 SalI G'TCGA_C Cuts at: 1936 5167 1936 Size: 3231 4594 SanDI GG'GwC_CC Cuts at: 4801 4801 Size: 7825 SapI GCTCTTCn'nnn_ Cuts at: 4428 4428 Size: 7825 SbfI CC_TGCA'GG Cuts at: 3955 5177 3955 Size: 1222 6603 ScaI AGT'ACT Cuts at: 4313 6073 7785 4313 Size: 1760 1712 4353 Fragments arranged by size: 4353 1760 1712 SexAI A'CCwGG_T Cuts at: 2286 2286 Size: 7825 SfcI C'TryA_G Cuts at: 826 1045 2399 2876 3072 3587 3951 4321 4830 Size: 219 1354 477 196 515 364 370 509 Cuts at: 4830 4989 5173 6308 6989 7180 826 Size: 159 184 1135 681 191 1471 Fragments arranged by size: 1471 1354 1135 681 515 509 477 370 364 219 196 191 184 159 SfoI GGC'GCC Cuts at: 4300 4300 Size: 7825 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7825 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7825 SmaI CCC'GGG Cuts at: 1872 2195 3332 4289 5152 1872 Size: 323 1137 957 863 4545 Fragments arranged by size: 4545 1137 957 863 323 SmlI C'TyrA_G Cuts at: 820 1017 1943 3550 4774 5929 6830 7092 820 Size: 197 926 1607 1224 1155 901 262 1553 Fragments arranged by size: 1607 1553 1224 1155 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5616 493 Size: 5123 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7825 SphI G_CATG'C Cuts at: 1132 2187 5183 1132 Size: 1055 2996 3774 Fragments arranged by size: 3774 2996 1055 SrfI GCCC'GGGC Cuts at: 2195 2195 Size: 7825 SspI AAT'ATT Cuts at: 5 52 5749 5 Size: 47 5697 2081 Fragments arranged by size: 5697 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7825 StyI C'CwwG_G Cuts at: 513 1065 2004 2307 2412 2937 3615 3745 3795 Size: 552 939 303 105 525 678 130 50 Cuts at: 3795 4455 5264 513 Size: 660 809 3074 Fragments arranged by size: 3074 939 809 678 660 552 525 303 130 105 50 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2734 3560 4605 5852 5869 6022 6207 Size: 1255 1154 826 1045 1247 17 153 185 Cuts at: 6207 6652 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1247 1154 1045 826 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2580 3756 3924 Size: 274 80 33 51 1443 603 1176 168 Cuts at: 3924 4311 4419 6071 7783 96 Size: 387 108 1652 1712 138 Fragments arranged by size: 1712 1652 1443 1176 603 387 274 168 138 108 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2543 2575 3831 3863 7785 7817 2543 Size: 32 1256 32 3922 32 2551 Fragments arranged by size: 3922 2551 1256 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7825 XbaI T'CTAG_A Cuts at: 5161 5161 Size: 7825 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2638 3160 3743 4072 2638 Size: 522 583 329 6391 Fragments arranged by size: 6391 583 522 329 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7825 XmaI C'CCGG_G Cuts at: 1870 2193 3330 4287 5150 1870 Size: 323 1137 957 863 4545 Fragments arranged by size: 4545 1137 957 863 323 XmnI GAAnn'nnTTC Cuts at: 5954 5954 Size: 7825 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5633 276 Size: 53 83 186 936 4099 2468 Fragments arranged by size: 4099 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BfrBI BplI BsiWI BstZ17I EcoRV FseI FspAI NruI NsiI PacI PspXI PsrI SfiI SwaI