Restriction map for
pFN21AE2505
(Linear) (Six-base) MAPSORT of: KIEE2505.seq Check: 9109 from: 1 to: 3120
KIEE2505.orf
With 182 enzymes: SgfI *
February 16, 2012 13:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 38 156 480 1468 1953 3120
Size: 38 118 324 988 485 1167
Fragments arranged by size:
1167 988 485 324 118 38
AccI GT'mk_AC
Cuts at: 0 323 2315 3120
Size: 323 1992 805
Fragments arranged by size:
1992 805 323
Acc65I G'GTAC_C
Cuts at: 0 229 1204 3120
Size: 229 975 1916
Fragments arranged by size:
1916 975 229
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 645 713 1239 2942 3120
Size: 50 595 68 526 1703 178
Fragments arranged by size:
1703 595 526 178 68 50
AflIII A'CryG_T
Cuts at: 0 1754 3120
Size: 1754 1366
AhdI GACnn_n'nnGTC
Cuts at: 0 1785 3120
Size: 1785 1335
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 982 3120
Size: 982 2138
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1126 1158 2029 2061 3120
Size: 1126 32 871 32 1059
Fragments arranged by size:
1126 1059 871 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 333 1012 2016 2920 3120
Size: 333 679 1004 904 200
Fragments arranged by size:
1004 904 679 333 200
ApaI G_GGCC'C
Cuts at: 0 2096 2393 2966 3120
Size: 2096 297 573 154
Fragments arranged by size:
2096 573 297 154
ApoI r'AATT_y
Cuts at: 0 400 2948 3120
Size: 400 2548 172
Fragments arranged by size:
2548 400 172
AvaI C'yCGr_G
Cuts at: 0 188 1167 1325 2282 2392 2618 3120
Size: 188 979 158 957 110 226 502
Fragments arranged by size:
979 957 502 226 188 158 110
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2194 2227 3120
Size: 2194 33 893
Fragments arranged by size:
2194 893 33
BamHI G'GATC_C
Cuts at: 0 88 2978 3120
Size: 88 2890 142
Fragments arranged by size:
2890 142 88
BanI G'GyrC_C
Cuts at: 0 229 352 904 1204 1411 2136 2286 2293
Size: 229 123 552 300 207 725 150 7
Cuts at: 2293 3064 3120
Size: 771 56
Fragments arranged by size:
771 725 552 300 229 207 150 123
56 7
BanII G_rGCy'C
Cuts at: 0 866 2096 2169 2180 2393 2435 2966 3119
Size: 866 1230 73 11 213 42 531 153
Cuts at: 3119 3120
Size: 1
Fragments arranged by size:
1230 866 531 213 153 73 42 11
1
BbeI G_GCGC'C
Cuts at: 0 2297 3120
Size: 2297 823
BbsI GAAGACnn'nnnn_
Cuts at: 0 125 651 1169 2930 3120
Size: 125 526 518 1761 190
Fragments arranged by size:
1761 526 518 190 125
BbvCI CC'TCA_GC
Cuts at: 0 2423 3120
Size: 2423 697
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1978 2012 2111 2145 3120
Size: 1978 34 99 34 975
Fragments arranged by size:
1978 975 99 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2536 3120
Size: 2536 584
BclI T'GATC_A
Cuts at: 0 1418 3120
Size: 1418 1702
BglI GCCn_nnn'nGGC
Cuts at: 0 984 1671 2091 3120
Size: 984 687 420 1029
Fragments arranged by size:
1029 984 687 420
BlpI GC'TnA_GC
Cuts at: 0 674 860 974 1153 1876 3120
Size: 674 186 114 179 723 1244
Fragments arranged by size:
1244 723 674 186 179 114
Bme1580I G_kGCm'C
Cuts at: 0 355 907 1676 2077 2096 2393 2619 2966
Size: 355 552 769 401 19 297 226 347
Cuts at: 2966 3120
Size: 154
Fragments arranged by size:
769 552 401 355 347 297 226 154
19
BmrI ACTGGGnnnn_n'
Cuts at: 0 340 418 2087 2298 2696 2908 3120
Size: 340 78 1669 211 398 212 212
Fragments arranged by size:
1669 398 340 212 212 211 78
BmtI G_CTAG'C
Cuts at: 0 1231 3120
Size: 1231 1889
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 265 796 1374 1388 1688 1902 2082
Size: 185 80 531 578 14 300 214 180
Cuts at: 2082 3120
Size: 1038
Fragments arranged by size:
1038 578 531 300 214 185 180 80
14
Bpu10I CC'TnA_GC
Cuts at: 0 2423 3120
Size: 2423 697
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1530 2754 3120
Size: 1530 1224 366
Fragments arranged by size:
1530 1224 366
BsaI GGTCTCn'nnnn_
Cuts at: 0 413 736 1693 2475 3120
Size: 413 323 957 782 645
Fragments arranged by size:
957 782 645 413 323
BsaAI yAC'GTr
Cuts at: 0 1757 3120
Size: 1757 1363
BsaBI GATnn'nnATC
Cuts at: 0 1309 2908 3094 3120
Size: 1309 1599 186 26
Fragments arranged by size:
1599 1309 186 26
BsaHI Gr'CG_yC
Cuts at: 0 543 1275 2194 2294 3120
Size: 543 732 919 100 826
Fragments arranged by size:
919 826 732 543 100
BsaWI w'CCGG_w
Cuts at: 0 1679 2108 3120
Size: 1679 429 1012
Fragments arranged by size:
1679 1012 429
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 154 184 1006 1036 1386 1416 1826 1856
Size: 154 30 822 30 350 30 410 30
Cuts at: 1856 3120
Size: 1264
Fragments arranged by size:
1264 822 410 350 154 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 51 163 175 427 496 2171 3120
Size: 51 112 12 252 69 1675 949
Fragments arranged by size:
1675 949 252 112 69 51 12
BseYI C'CCAG_C
Cuts at: 0 443 715 735 880 1129 1730 2016 2560
Size: 443 272 20 145 249 601 286 544
Cuts at: 2560 3048 3120
Size: 488 72
Fragments arranged by size:
601 544 488 443 286 272 249 145
72 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 970 988 1064 1991 2390 2527 3120
Size: 970 18 76 927 399 137 593
Fragments arranged by size:
970 927 593 399 137 76 18
BsiHKAI G_wGCw'C
Cuts at: 0 1352 2180 3119 3120
Size: 1352 828 939 1
Fragments arranged by size:
1352 939 828 1
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2216 3120
Size: 2216 904
Bsp1286I G_dGCh'C
Cuts at: 0 355 866 907 1352 1676 2077 2096 2169
Size: 355 511 41 445 324 401 19 73
Cuts at: 2169 2180 2393 2435 2619 2966 3119 3120
Size: 11 213 42 184 347 153 1
Fragments arranged by size:
511 445 401 355 347 324 213 184
153 73 42 41 19 11 1
BspEI T'CCGG_A
Cuts at: 0 2108 3120
Size: 2108 1012
BspHI T'CATG_A
Cuts at: 0 290 435 3120
Size: 290 145 2685
Fragments arranged by size:
2685 290 145
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 38 156 480 756 1037 1145 1468 1953
Size: 38 118 324 276 281 108 323 485
Cuts at: 1953 2246 3120
Size: 293 874
Fragments arranged by size:
874 485 324 323 293 281 276 118
108 38
BsrDI GCAATG_nn'
Cuts at: 0 578 2001 3120
Size: 578 1423 1119
Fragments arranged by size:
1423 1119 578
BsrFI r'CCGG_y
Cuts at: 0 1051 1436 2833 2890 3120
Size: 1051 385 1397 57 230
Fragments arranged by size:
1397 1051 385 230 57
BsrGI T'GTAC_A
Cuts at: 0 575 3120
Size: 575 2545
BssSI C'ACGA_G
Cuts at: 0 2410 3120
Size: 2410 710
BstAPI GCAn_nnn'nTGC
Cuts at: 0 171 313 3120
Size: 171 142 2807
Fragments arranged by size:
2807 171 142
BstEII G'GTnAC_C
Cuts at: 0 768 3120
Size: 768 2352
BstXI CCAn_nnnn'nTGG
Cuts at: 0 348 636 1806 3120
Size: 348 288 1170 1314
Fragments arranged by size:
1314 1170 348 288
BstYI r'GATC_y
Cuts at: 0 88 875 1304 1710 2360 2543 2978 3120
Size: 88 787 429 406 650 183 435 142
Fragments arranged by size:
787 650 435 429 406 183 142 88
Bsu36I CC'TnA_GG
Cuts at: 0 2142 3120
Size: 2142 978
BtgI C'CryG_G
Cuts at: 0 155 1286 1293 1343 1610 1740 1887 2379
Size: 155 1131 7 50 267 130 147 492
Cuts at: 2379 2734 3083 3120
Size: 355 349 37
Fragments arranged by size:
1131 492 355 349 267 155 147 130
50 37 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1896 3103 3120
Size: 1896 1207 17
Fragments arranged by size:
1896 1207 17
BtsI GCAGTG_nn'
Cuts at: 0 853 2402 3120
Size: 853 1549 718
Fragments arranged by size:
1549 853 718
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1325 1360 3120
Size: 1325 35 1760
Fragments arranged by size:
1760 1325 35
DraIII CAC_nnn'GTG
Cuts at: 0 552 3000 3084 3120
Size: 552 2448 84 36
Fragments arranged by size:
2448 552 84 36
DrdI GACnn_nn'nnGTC
Cuts at: 0 2199 2803 3120
Size: 2199 604 317
Fragments arranged by size:
2199 604 317
EaeI y'GGCC_r
Cuts at: 0 623 2931 3120
Size: 623 2308 189
Fragments arranged by size:
2308 623 189
EarI CTCTTCn'nnn_
Cuts at: 0 69 2423 3120
Size: 69 2354 697
Fragments arranged by size:
2354 697 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 937 1808 3034 3120
Size: 937 871 1226 86
Fragments arranged by size:
1226 937 871 86
EcoICRI GAG'CTC
Cuts at: 0 2178 3117 3120
Size: 2178 939 3
Fragments arranged by size:
2178 939 3
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 185 265 645 713 796 1239 1374
Size: 50 135 80 380 68 83 443 135
Cuts at: 1374 1388 1688 1902 2082 2942 3120
Size: 14 300 214 180 860 178
Fragments arranged by size:
860 443 380 300 214 180 178 135
135 83 80 68 50 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2250 3120
Size: 2250 870
EcoO109I rG'GnC_Cy
Cuts at: 0 1134 1825 2093 2666 2796 2962 3120
Size: 1134 691 268 573 130 166 158
Fragments arranged by size:
1134 691 573 268 166 158 130
EcoRI G'AATT_C
Cuts at: 0 400 3120
Size: 400 2720
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 675 3120
Size: 675 2445
HaeII r_GCGC'y
Cuts at: 0 1223 2297 3120
Size: 1223 1074 823
Fragments arranged by size:
1223 1074 823
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1006 1038 1386 1418 1771 1803 2165 2197
Size: 1006 32 348 32 353 32 362 32
Cuts at: 2197 3120
Size: 923
Fragments arranged by size:
1006 923 362 353 348 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1380 2187 3120
Size: 1380 807 933
Fragments arranged by size:
1380 933 807
KasI G'GCGC_C
Cuts at: 0 2293 3120
Size: 2293 827
KpnI G_GTAC'C
Cuts at: 0 233 1208 3120
Size: 233 975 1912
Fragments arranged by size:
1912 975 233
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 365 1049 3120
Size: 365 684 2071
Fragments arranged by size:
2071 684 365
MscI TGG'CCA
Cuts at: 0 625 2933 3120
Size: 625 2308 187
Fragments arranged by size:
2308 625 187
MslI CAynn'nnrTG
Cuts at: 0 964 1759 2719 3019 3025 3120
Size: 964 795 960 300 6 95
Fragments arranged by size:
964 960 795 300 95 6
MspA1I CmG'CkG
Cuts at: 0 267 500 859 1288 1295 1734 1880 2153
Size: 267 233 359 429 7 439 146 273
Cuts at: 2153 2381 2422 2578 2627 2736 3120
Size: 228 41 156 49 109 384
Fragments arranged by size:
439 429 384 359 273 267 233 228
156 146 109 49 41 7
NaeI GCC'GGC
Cuts at: 0 1053 3120
Size: 1053 2067
NarI GG'CG_CC
Cuts at: 0 2294 3120
Size: 2294 826
NcoI C'CATG_G
Cuts at: 0 1610 1740 3120
Size: 1610 130 1380
Fragments arranged by size:
1610 1380 130
NgoMIV G'CCGG_C
Cuts at: 0 1051 3120
Size: 1051 2069
NheI G'CTAG_C
Cuts at: 0 1227 3120
Size: 1227 1893
NspI r_CATG'y
Cuts at: 0 182 1176 3120
Size: 182 994 1944
Fragments arranged by size:
1944 994 182
PasI CC'CwG_GG
Cuts at: 0 84 2435 3120
Size: 84 2351 685
Fragments arranged by size:
2351 685 84
PflMI CCAn_nnn'nTGG
Cuts at: 0 270 333 1185 1773 2456 2940 3061 3084
Size: 270 63 852 588 683 484 121 23
Cuts at: 3084 3120
Size: 36
Fragments arranged by size:
852 683 588 484 270 121 63 36
23
PfoI T'CCnGG_A
Cuts at: 0 2758 3120
Size: 2758 362
PmlI CAC'GTG
Cuts at: 0 1757 3120
Size: 1757 1363
PpuMI rG'GwC_Cy
Cuts at: 0 1134 1825 2796 3120
Size: 1134 691 971 324
Fragments arranged by size:
1134 971 691 324
PshAI GACnn'nnGTC
Cuts at: 0 542 3120
Size: 542 2578
PspOMI G'GGCC_C
Cuts at: 0 2092 2389 2962 3120
Size: 2092 297 573 158
Fragments arranged by size:
2092 573 297 158
PstI C_TGCA'G
Cuts at: 0 398 875 1950 2829 2988 3120
Size: 398 477 1075 879 159 132
Fragments arranged by size:
1075 879 477 398 159 132
PvuII CAG'CTG
Cuts at: 0 267 859 1734 1880 2422 2578 3120
Size: 267 592 875 146 542 156 542
Fragments arranged by size:
875 592 542 542 267 156 146
RsrII CG'GwC_CG
Cuts at: 0 2583 3120
Size: 2583 537
SacI G_AGCT'C
Cuts at: 0 2180 3119 3120
Size: 2180 939 1
Fragments arranged by size:
2180 939 1
SacII CC_GC'GG
Cuts at: 0 1289 1296 2382 2737 3120
Size: 1289 7 1086 355 383
Fragments arranged by size:
1289 1086 383 355 7
SanDI GG'GwC_CC
Cuts at: 0 2796 3120
Size: 2796 324
SapI GCTCTTCn'nnn_
Cuts at: 0 2423 3120
Size: 2423 697
SbfI CC_TGCA'GG
Cuts at: 0 1950 3120
Size: 1950 1170
ScaI AGT'ACT
Cuts at: 0 2308 3120
Size: 2308 812
SexAI A'CCwGG_T
Cuts at: 0 281 3120
Size: 281 2839
SfcI C'TryA_G
Cuts at: 0 394 871 1067 1582 1946 2316 2825 2984
Size: 394 477 196 515 364 370 509 159
Cuts at: 2984 3120
Size: 136
Fragments arranged by size:
515 509 477 394 370 364 196 159
136
SfoI GGC'GCC
Cuts at: 0 2295 3120
Size: 2295 825
SmaI CCC'GGG
Cuts at: 0 190 1327 2284 3120
Size: 190 1137 957 836
Fragments arranged by size:
1137 957 836 190
SmlI C'TyrA_G
Cuts at: 0 1545 2769 3120
Size: 1545 1224 351
Fragments arranged by size:
1545 1224 351
SphI G_CATG'C
Cuts at: 0 182 3120
Size: 182 2938
SrfI GCCC'GGGC
Cuts at: 0 190 3120
Size: 190 2930
StyI C'CwwG_G
Cuts at: 0 302 407 932 1610 1740 1790 2450 3120
Size: 302 105 525 678 130 50 660 670
Fragments arranged by size:
678 670 660 525 302 130 105 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 729 1555 2600 3120
Size: 729 826 1045 520
Fragments arranged by size:
1045 826 729 520
TatI w'GTAC_w
Cuts at: 0 575 1751 1919 2306 2414 3120
Size: 575 1176 168 387 108 706
Fragments arranged by size:
1176 706 575 387 168 108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 538 570 1826 1858 3120
Size: 538 32 1256 32 1262
Fragments arranged by size:
1262 1256 538 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 633 1155 1738 2067 3120
Size: 633 522 583 329 1053
Fragments arranged by size:
1053 633 583 522 329
XmaI C'CCGG_G
Cuts at: 0 188 1325 2282 3120
Size: 188 1137 957 838
Fragments arranged by size:
1137 957 838 188
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AhdI AlfI AloI
AlwNI ApaI ApoI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BlpI
Bme1580I BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BsrGI
BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraIII DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII
KasI KpnI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PasI PflMI PfoI PmlI
PpuMI PshAI PspOMI PstI PvuII RsrII SacI SacII
SanDI SapI SbfI ScaI SexAI SfcI SfoI SmaI
SmlI SphI SrfI StyI TaqII TatI TstI XcmI
XmaI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AleI ApaLI AscI
AseI AsiSI AvrII BfrBI BglII BmgBI BplI BsiEI
BsiWI BsmI BsrBI BssHII BstBI BstZ17I ClaI DraI
EagI EcoRV FseI FspI FspAI HindIII HpaI MfeI
MluI NdeI NotI NruI NsiI PacI PciI PmeI
PpiI PsiI PspXI PsrI PvuI SalI SfiI SgfI
SgrAI SnaBI SpeI SspI StuI SwaI Tth111I XbaI
XhoI XmnI ZraI