Restriction map for
pFN21ASDB1439
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# NO CUT ENZYMES ( 50 enzymes) #
##################################
AclI AflII AgeI Alw44I ApaLI
AscI AseI AsnI AspI AvrII
BfrI BlnI BsiWI BssHII Bst1107I
BstBI BstZ17I CpoI DraI FseI
HindIII HpaI KspI MfeI MluI
MunI NheI NotI NruI NspV
PacI PflFI PinAI PmeI PshBI
Psp1406I PvuI RsrII SacI SacII
SfiI SfuI SgrAI SmaI SnaBI
SpeI SwaI Tth111I XmaCI XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AatII 4294 GACGTC GACGT^C
Acc65I 2564 GGTACC G^GTACC
AccII 658 CGCG CG^CG
AhdI 6756 GACTGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 632 AGCGCT AGC^GCT
Asp700I 5272 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2564 GGTACC G^GTACC
AspEI 6756 GACTGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3479 TGCGCA TGC^GCA
BsiEI 868 CGGCCG CG[GA][CT]^CG
Bsp1407I 1196 TGTACA T^GTACA
BspDI 6067 ATCGAT AT^CGAT
BsrGI 1196 TGTACA T^GTACA
BssSI 85 CTCGTG CACGAG(-5/-1)
BstUI 658 CGCG CG^CG
ClaI 6067 ATCGAT AT^CGAT
DraIII 3555 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 868 CGGCCG C^GGCCG
Eam1105I 6756 GACTGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 868 CGGCCG C^GGCCG
Eco47III 632 AGCGCT AGC^GCT
Eco52I 868 CGGCCG C^GGCCG
EcoRV 463 GATATC GAT^ATC
FspI 3479 TGCGCA TGC^GCA
KpnI 2564 GGTACC GGTAC^C
MvnI 658 CGCG CG^CG
NcoI 5642 CCATGG C^CATGG
NdeI 3725 CATATG CA^TATG
SalI 6803 GTCGAC G^TCGAC
SbfI 4460 CCTGCAGG CCTGCA^GG
ScaI 6698 AGTACT AGT^ACT
Sse8387I 4460 CCTGCAGG CCTGCA^GG
SspBI 1196 TGTACA T^GTACA
XbaI 4824 TCTAGA T^CTAGA
XmnI 5272 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 33 enzymes) #
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AccIII 416 TCCGGA T^CCGGA
4350 TCCGGA
AlwNI 1123 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
4066 CAGGCGCTG
AocI 4930 CCTAAGG CC^T[ACGT]AGG
5975 CCTCAGG
BamHI 1068 GGATCC G^GATCC
2715 GGATCC
BbeI 3969 GGCGCC GGCGC^C
6436 GGCGCC
BbrPI 1696 CACGTG CAC^GTG
3558 CACGTG
BbvCI 185 CCTCAGC CCTCAGC(-5/-2)
5484 GCTGAGG
BglII 811 AGATCT A^GATCT
1310 AGATCT
BseAI 416 TCCGGA T^CCGGA
4350 TCCGGA
BspEI 416 TCCGGA T^CCGGA
4350 TCCGGA
BspLU11I 1634 ACATGT A^CATGT
1664 ACATGT
BsrFI 3972 GCCGGC [GA]^CCGG[CT]
5926 GCCGGC
BstEII 894 GGTGACC G^GT[ACGT]ACC
5172 GGTGACC
BstPI 894 GGTGACC G^GT[ACGT]ACC
5172 GGTGACC
Bsu36I 4930 CCTAAGG CC^T[ACGT]AGG
5975 CCTCAGG
Cfr10I 3972 GCCGGC [GA]^CCGG[CT]
5926 GCCGGC
DrdI 523 GACTCAGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3946 GACTCCAATGTC
Eco81I 4930 CCTAAGG CC^T[ACGT]AGG
5975 CCTCAGG
EcoO65I 894 GGTGACC G^GT[ACGT]ACC
5172 GGTGACC
EcoRI 848 GAATTC G^AATTC
2497 GAATTC
EcoT22I 1112 ATGCAT ATGCA^T
2158 ATGCAT
KasI 3969 GGCGCC G^GCGCC
6436 GGCGCC
MroI 416 TCCGGA T^CCGGA
4350 TCCGGA
NaeI 3972 GCCGGC GCC^GGC
5926 GCCGGC
NarI 3969 GGCGCC GG^CGCC
6436 GGCGCC
NgoMIV 3972 GCCGGC G^CCGGC
5926 GCCGGC
NsiI 1112 ATGCAT ATGCA^T
2158 ATGCAT
PaeR7I 3285 CTCGAG C^TCGAG
6785 CTCGAG
PmaCI 1696 CACGTG CAC^GTG
3558 CACGTG
PmlI 1696 CACGTG CAC^GTG
3558 CACGTG
SfoI 3969 GGCGCC GGC^GCC
6436 GGCGCC
SspI 825 AATATT AAT^ATT
6016 AATATT
XhoI 3285 CTCGAG C^TCGAG
6785 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AluI AlwI ApaI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcgI BciVI
BclI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspHI BspMI BsrBI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PshAI PstI
PvuII RcaI RsaI SapI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmlI SphI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII