Restriction map for
pFN21ASDB1439
(Linear) (Six-base) MAPSORT of: KIBB1439.seq Check: 8335 from: 1 to: 6819
KIBB1439.orf
With 182 enzymes: SgfI *
February 28, 2007 11:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1221 4454 6819
Size: 1221 3233 2365
Fragments arranged by size:
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AatII G_ACGT'C
Cuts at: 0 4298 6819
Size: 4298 2521
AccI GT'mk_AC
Cuts at: 0 533 6185 6804 6819
Size: 533 5652 619 15
Fragments arranged by size:
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Acc65I G'GTAC_C
Cuts at: 0 2564 6819
Size: 2564 4255
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 619 630 1252 1445 2065 2389 3334 3460
Size: 619 11 622 193 620 324 945 126
Cuts at: 3460 3841 4233 4354 4853 5012 5786 6044 6819
Size: 381 392 121 499 159 774 258 775
Fragments arranged by size:
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381 324 258 193 159 126 121 11
AfeI AGC'GCT
Cuts at: 0 634 6819
Size: 634 6185
AflIII A'CryG_T
Cuts at: 0 1266 1634 1664 3559 6819
Size: 1266 368 30 1895 3260
Fragments arranged by size:
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AhdI GACnn_n'nnGTC
Cuts at: 0 6761 6819
Size: 6761 58
AleI CACnn'nnGTG
Cuts at: 0 5029 5968 6819
Size: 5029 939 851
Fragments arranged by size:
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AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1621 1653 2589 2621 3674 3706 6819
Size: 1621 32 936 32 1053 32 3113
Fragments arranged by size:
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AlwNI CAG_nnn'CTG
Cuts at: 0 1128 4071 6819
Size: 1128 2943 2748
Fragments arranged by size:
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ApaI G_GGCC'C
Cuts at: 0 1307 1456 4777 6819
Size: 1307 149 3321 2042
Fragments arranged by size:
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ApoI r'AATT_y
Cuts at: 0 848 2497 3788 4559 4645 4676 6478 6738
Size: 848 1649 1291 771 86 31 1802 260
Cuts at: 6738 6819
Size: 81
Fragments arranged by size:
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31
AvaI C'yCGr_G
Cuts at: 0 979 995 1457 3285 4507 4945 6785 6819
Size: 979 16 462 1828 1222 438 1840 34
Fragments arranged by size:
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BamHI G'GATC_C
Cuts at: 0 1068 2715 6819
Size: 1068 1647 4104
Fragments arranged by size:
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BanI G'GyrC_C
Cuts at: 0 703 2564 3424 3969 4513 5845 5923 6436
Size: 703 1861 860 545 544 1332 78 513
Cuts at: 6436 6454 6819
Size: 18 365
Fragments arranged by size:
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78 18
BanII G_rGCy'C
Cuts at: 0 1307 1456 2679 2696 2819 4777 4945 5158
Size: 1307 149 1223 17 123 1958 168 213
Cuts at: 5158 5726 5738 6290 6819
Size: 568 12 552 529
Fragments arranged by size:
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149 123 17 12
BbeI G_GCGC'C
Cuts at: 0 3973 6440 6819
Size: 3973 2467 379
Fragments arranged by size:
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BbsI GAAGACnn'nnnn_
Cuts at: 0 728 1028 4390 4841 5496 6819
Size: 728 300 3362 451 655 1323
Fragments arranged by size:
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BbvCI CC'TCA_GC
Cuts at: 0 186 5485 6819
Size: 186 5299 1334
Fragments arranged by size:
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BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 49 83 3887 3921 5220 5254 6130 6164
Size: 49 34 3804 34 1299 34 876 34
Cuts at: 6164 6819
Size: 655
Fragments arranged by size:
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34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1220 1679 4622 6819
Size: 1220 459 2943 2197
Fragments arranged by size:
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BclI T'GATC_A
Cuts at: 0 542 1349 1943 2571 6819
Size: 542 807 594 628 4248
Fragments arranged by size:
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BfrBI ATG'CAT
Cuts at: 0 1114 2160 6819
Size: 1114 1046 4659
Fragments arranged by size:
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BglI GCCn_nnn'nGGC
Cuts at: 0 2278 5290 5377 6203 6819
Size: 2278 3012 87 826 616
Fragments arranged by size:
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BglII A'GATC_T
Cuts at: 0 811 1310 6819
Size: 811 499 5509
Fragments arranged by size:
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BlpI GC'TnA_GC
Cuts at: 0 2251 2363 3062 3869 6819
Size: 2251 112 699 807 2950
Fragments arranged by size:
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Bme1580I G_kGCm'C
Cuts at: 0 706 1307 1456 1614 4018 4507 4777 5848
Size: 706 601 149 158 2404 489 270 1071
Cuts at: 5848 6457 6819
Size: 609 362
Fragments arranged by size:
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158 149
BmgBI CAC'GTC
Cuts at: 0 1728 3573 6819
Size: 1728 1845 3246
Fragments arranged by size:
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BmrI ACTGGGnnnn_n'
Cuts at: 0 4693 5178 5622 5966 6819
Size: 4693 485 444 344 853
Fragments arranged by size:
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BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4711 5019 5610 6717 6819
Size: 4711 308 591 1107 102
Fragments arranged by size:
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BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 649 1150 1515 1570 1645 1868 1885
Size: 193 456 501 365 55 75 223 17
Cuts at: 1885 3139 3572 3769 4039 5119 5252 5835 5912
Size: 1254 433 197 270 1080 133 583 77
Cuts at: 5912 6177 6601 6815 6819
Size: 265 424 214 4
Fragments arranged by size:
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270 265 223 214 197 193 133 77
75 55 17 4
Bpu10I CC'TnA_GC
Cuts at: 0 186 3176 3324 3420 5467 5485 6819
Size: 186 2990 148 96 2047 18 1334
Fragments arranged by size:
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BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 536 779 5832 6454 6819
Size: 536 243 5053 622 365
Fragments arranged by size:
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BsaI GGTCTCn'nnnn_
Cuts at: 0 922 2044 3778 6633 6819
Size: 922 1122 1734 2855 186
Fragments arranged by size:
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BsaAI yAC'GTr
Cuts at: 0 1698 3560 6209 6819
Size: 1698 1862 2649 610
Fragments arranged by size:
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BsaBI GATnn'nnATC
Cuts at: 0 949 2131 2152 6819
Size: 949 1182 21 4667
Fragments arranged by size:
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BsaHI Gr'CG_yC
Cuts at: 0 3970 4295 6437 6819
Size: 3970 325 2142 382
Fragments arranged by size:
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BsaWI w'CCGG_w
Cuts at: 0 416 1802 2394 4350 5456 6819
Size: 416 1386 592 1956 1106 1363
Fragments arranged by size:
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BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1984 2014 2020 2050 2098 2128 6819
Size: 1984 30 6 30 48 30 4691
Fragments arranged by size:
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BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 325 3121 3766 4963 5602 6621 6624 6627
Size: 325 2796 645 1197 639 1019 3 3
Cuts at: 6627 6652 6655 6709 6819
Size: 25 3 54 110
Fragments arranged by size:
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25 3 3 3
BseYI C'CCAG_C
Cuts at: 0 2899 3083 3113 4412 5079 5215 5337 5362
Size: 2899 184 30 1299 667 136 122 25
Cuts at: 5362 5856 6819
Size: 494 963
Fragments arranged by size:
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30 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1891 4084 4488 6610 6763 6819
Size: 1891 2193 404 2122 153 56
Fragments arranged by size:
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BsiEI CG_ry'CG
Cuts at: 0 871 6819
Size: 871 5948
BsiHKAI G_wGCw'C
Cuts at: 0 691 1004 2726 2963 3068 4136 5594 6819
Size: 691 313 1722 237 105 1068 1458 1225
Fragments arranged by size:
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BsmI GAATG_Cn'
Cuts at: 0 135 163 200 348 1242 2878 6819
Size: 135 28 37 148 894 1636 3941
Fragments arranged by size:
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BsmBI CGTCTCn'nnnn_
Cuts at: 0 648 3282 3748 6819
Size: 648 2634 466 3071
Fragments arranged by size:
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Bsp1286I G_dGCh'C
Cuts at: 0 691 706 1004 1307 1456 1614 2679 2696
Size: 691 15 298 303 149 158 1065 17
Cuts at: 2696 2726 2819 2963 3068 4018 4136 4507 4777
Size: 30 93 144 105 950 118 371 270
Cuts at: 4777 4945 5158 5594 5726 5738 5848 6290 6457
Size: 168 213 436 132 12 110 442 167
Cuts at: 6457 6819
Size: 362
Fragments arranged by size:
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298 270 213 168 167 158 149 144
132 118 110 105 93 30 17 15
12
BspEI T'CCGG_A
Cuts at: 0 416 4350 6819
Size: 416 3934 2469
Fragments arranged by size:
3934 2469 416
BspHI T'CATG_A
Cuts at: 0 153 338 1940 4757 5195 6765 6819
Size: 153 185 1602 2817 438 1570 54
Fragments arranged by size:
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BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1221 2327 3535 4454 6539 6819
Size: 1221 1106 1208 919 2085 280
Fragments arranged by size:
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BsrBI CCG'CTC
Cuts at: 0 141 4769 5112 6819
Size: 141 4628 343 1707
Fragments arranged by size:
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BsrDI GCAATG_nn'
Cuts at: 0 231 3218 3386 3395 6819
Size: 231 2987 168 9 3424
Fragments arranged by size:
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BsrFI r'CCGG_y
Cuts at: 0 3972 5926 6819
Size: 3972 1954 893
Fragments arranged by size:
3972 1954 893
BsrGI T'GTAC_A
Cuts at: 0 1196 6819
Size: 1196 5623
BssSI C'ACGA_G
Cuts at: 0 85 6819
Size: 85 6734
BstAPI GCAn_nnn'nTGC
Cuts at: 0 885 2526 4071 4131 4328 6819
Size: 885 1641 1545 60 197 2491
Fragments arranged by size:
2491 1641 1545 885 197 60
BstEII G'GTnAC_C
Cuts at: 0 894 5172 6819
Size: 894 4278 1647
Fragments arranged by size:
4278 1647 894
BstXI CCAn_nnnn'nTGG
Cuts at: 0 364 2609 5031 5649 6300 6819
Size: 364 2245 2422 618 651 519
Fragments arranged by size:
2422 2245 651 618 519 364
BstYI r'GATC_y
Cuts at: 0 215 811 1068 1310 1709 1988 2715 3303
Size: 215 596 257 242 399 279 727 588
Cuts at: 3303 6650 6819
Size: 3347 169
Fragments arranged by size:
3347 727 596 588 399 279 257 242
215 169
Bsu36I CC'TnA_GG
Cuts at: 0 4931 5976 6819
Size: 4931 1045 843
Fragments arranged by size:
4931 1045 843
BtgI C'CryG_G
Cuts at: 0 202 1790 2483 5642 6819
Size: 202 1588 693 3159 1177
Fragments arranged by size:
3159 1588 1177 693 202
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 193 591 6231 6819
Size: 193 398 5640 588
Fragments arranged by size:
5640 588 398 193
BtsI GCAGTG_nn'
Cuts at: 0 2087 2222 2464 3522 4275 5293 5424 5867
Size: 2087 135 242 1058 753 1018 131 443
Cuts at: 5867 6591 6819
Size: 724 228
Fragments arranged by size:
2087 1058 1018 753 724 443 242 228
135 131
ClaI AT'CG_AT
Cuts at: 0 6068 6819
Size: 6068 751
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3644 3679 6819
Size: 3644 35 3140
Fragments arranged by size:
3644 3140 35
DraIII CAC_nnn'GTG
Cuts at: 0 3560 6819
Size: 3560 3259
DrdI GACnn_nn'nnGTC
Cuts at: 0 529 3952 6819
Size: 529 3423 2867
Fragments arranged by size:
3423 2867 529
EaeI y'GGCC_r
Cuts at: 0 366 868 1481 2823 4749 6419 6819
Size: 366 502 613 1342 1926 1670 400
Fragments arranged by size:
1926 1670 1342 613 502 400 366
EagI C'GGCC_G
Cuts at: 0 868 6819
Size: 868 5951
EarI CTCTTCn'nnn_
Cuts at: 0 438 763 2008 2203 2432 3164 3439 3803
Size: 438 325 1245 195 229 732 275 364
Cuts at: 3803 6819
Size: 3016
Fragments arranged by size:
3016 1245 732 438 364 325 275 229
195
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1519 1891 6819
Size: 1519 372 4928
Fragments arranged by size:
4928 1519 372
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 619 630 649 1150 1252 1445 1515
Size: 193 426 11 19 501 102 193 70
Cuts at: 1515 1570 1645 1868 1885 2065 2389 3139 3334
Size: 55 75 223 17 180 324 750 195
Cuts at: 3334 3460 3572 3769 3841 4039 4233 4354 4853
Size: 126 112 197 72 198 194 121 499
Cuts at: 4853 5012 5119 5252 5786 5835 5912 6044 6177
Size: 159 107 133 534 49 77 132 133
Cuts at: 6177 6601 6815 6819
Size: 424 214 4
Fragments arranged by size:
750 534 501 499 426 424 324 223
214 198 197 195 194 193 193 180
159 133 133 132 126 121 112 107
102 77 75 72 70 55 49 19
17 11 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 697 1447 2572 2701 4930 4990 6819
Size: 697 750 1125 129 2229 60 1829
Fragments arranged by size:
2229 1829 1125 750 697 129 60
EcoO109I rG'GnC_Cy
Cuts at: 0 621 902 1303 1304 1452 1453 3180 3524
Size: 621 281 401 1 148 1 1727 344
Cuts at: 3524 3847 3913 4521 4773 4774 5529 5616 6819
Size: 323 66 608 252 1 755 87 1203
Fragments arranged by size:
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281 252 148 87 66 1 1 1
EcoRI G'AATT_C
Cuts at: 0 848 2497 6819
Size: 848 1649 4322
Fragments arranged by size:
4322 1649 848
EcoRV GAT'ATC
Cuts at: 0 465 6819
Size: 465 6354
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 87 2070 3465 4332 4363 6456 6819
Size: 87 1983 1395 867 31 2093 363
Fragments arranged by size:
2093 1983 1395 867 363 87 31
FspI TGC'GCA
Cuts at: 0 3481 6819
Size: 3481 3338
HaeII r_GCGC'y
Cuts at: 0 598 636 3869 3973 4072 5694 6440 6819
Size: 598 38 3233 104 99 1622 746 379
Fragments arranged by size:
3233 1622 746 598 379 104 99 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 534 566 1668 1700 2098 2130 3268 3281
Size: 534 32 1102 32 398 32 1138 13
Cuts at: 3281 3300 3313 3872 3904 5303 5335 6747 6779
Size: 19 13 559 32 1399 32 1412 32
Cuts at: 6779 6819
Size: 40
Fragments arranged by size:
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32 32 32 32 32 32 19 13
13
HincII GTy'rAC
Cuts at: 0 3891 4446 6805 6819
Size: 3891 555 2359 14
Fragments arranged by size:
3891 2359 555 14
KasI G'GCGC_C
Cuts at: 0 3969 6436 6819
Size: 3969 2467 383
Fragments arranged by size:
3969 2467 383
KpnI G_GTAC'C
Cuts at: 0 2568 6819
Size: 2568 4251
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1869 3116 4113 4616 5859 6819
Size: 1869 1247 997 503 1243 960
Fragments arranged by size:
1869 1247 1243 997 960 503
MscI TGG'CCA
Cuts at: 0 1483 2825 6421 6819
Size: 1483 1342 3596 398
Fragments arranged by size:
3596 1483 1342 398
MslI CAynn'nnrTG
Cuts at: 0 1339 1726 2008 2125 2233 2521 3340 3575
Size: 1339 387 282 117 108 288 819 235
Cuts at: 3575 4762 4846 5029 5053 5326 5647 5968 6298
Size: 1187 84 183 24 273 321 321 330
Cuts at: 6298 6819
Size: 521
Fragments arranged by size:
1339 1187 819 521 387 330 321 321
288 282 273 235 183 117 108 84
24
MspA1I CmG'CkG
Cuts at: 0 1958 2362 2833 3087 3117 3533 4047 4328
Size: 1958 404 471 254 30 416 514 281
Cuts at: 4328 4416 6270 6819
Size: 88 1854 549
Fragments arranged by size:
1958 1854 549 514 471 416 404 281
254 88 30
NaeI GCC'GGC
Cuts at: 0 3974 5928 6819
Size: 3974 1954 891
Fragments arranged by size:
3974 1954 891
NarI GG'CG_CC
Cuts at: 0 3970 6437 6819
Size: 3970 2467 382
Fragments arranged by size:
3970 2467 382
NcoI C'CATG_G
Cuts at: 0 5642 6819
Size: 5642 1177
NdeI CA'TA_TG
Cuts at: 0 3726 6819
Size: 3726 3093
NgoMIV G'CCGG_C
Cuts at: 0 3972 5926 6819
Size: 3972 1954 893
Fragments arranged by size:
3972 1954 893
NsiI A_TGCA'T
Cuts at: 0 1116 2162 6819
Size: 1116 1046 4657
Fragments arranged by size:
4657 1116 1046
NspI r_CATG'y
Cuts at: 0 1638 1668 3021 3072 3198 4063 4876 6324
Size: 1638 30 1353 51 126 865 813 1448
Cuts at: 6324 6615 6819
Size: 291 204
Fragments arranged by size:
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51 30
PasI CC'CwG_GG
Cuts at: 0 699 1362 3402 3964 6819
Size: 699 663 2040 562 2855
Fragments arranged by size:
2855 2040 699 663 562
PciI A'CATG_T
Cuts at: 0 1634 1664 6819
Size: 1634 30 5155
Fragments arranged by size:
5155 1634 30
PflMI CCAn_nnn'nTGG
Cuts at: 0 405 1128 2950 4318 5168 6387 6819
Size: 405 723 1822 1368 850 1219 432
Fragments arranged by size:
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PfoI T'CCnGG_A
Cuts at: 0 270 584 626 1562 2102 2583 3599 5105
Size: 270 314 42 936 540 481 1016 1506
Cuts at: 5105 6819
Size: 1714
Fragments arranged by size:
1714 1506 1016 936 540 481 314 270
42
PmlI CAC'GTG
Cuts at: 0 1698 3560 6819
Size: 1698 1862 3259
Fragments arranged by size:
3259 1862 1698
PpuMI rG'GwC_Cy
Cuts at: 0 3180 3524 3847 3913 5616 6819
Size: 3180 344 323 66 1703 1203
Fragments arranged by size:
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PshAI GACnn'nnGTC
Cuts at: 0 3770 4441 5566 6819
Size: 3770 671 1125 1253
Fragments arranged by size:
3770 1253 1125 671
PsiI TTA'TAA
Cuts at: 0 4624 6819
Size: 4624 2195
PspOMI G'GGCC_C
Cuts at: 0 1303 1452 4773 6819
Size: 1303 149 3321 2046
Fragments arranged by size:
3321 2046 1303 149
PspXI vC'TCGA_Gb
Cuts at: 0 6785 6819
Size: 6785 34
PstI C_TGCA'G
Cuts at: 0 616 1218 1835 2459 3532 4465 6819
Size: 616 602 617 624 1073 933 2354
Fragments arranged by size:
2354 1073 933 624 617 616 602
PvuII CAG'CTG
Cuts at: 0 2833 3087 3117 3533 4047 4416 6819
Size: 2833 254 30 416 514 369 2403
Fragments arranged by size:
2833 2403 514 416 369 254 30
SalI G'TCGA_C
Cuts at: 0 6803 6819
Size: 6803 16
SanDI GG'GwC_CC
Cuts at: 0 3913 6819
Size: 3913 2906
SapI GCTCTTCn'nnn_
Cuts at: 0 763 2008 2432 6819
Size: 763 1245 424 4387
Fragments arranged by size:
4387 1245 763 424
SbfI CC_TGCA'GG
Cuts at: 0 4465 6819
Size: 4465 2354
ScaI AGT'ACT
Cuts at: 0 6700 6819
Size: 6700 119
SexAI A'CCwGG_T
Cuts at: 0 2095 2423 6725 6819
Size: 2095 328 4302 94
Fragments arranged by size:
4302 2095 328 94
SfcI C'TryA_G
Cuts at: 0 144 612 709 1214 1831 2289 2455 3528
Size: 144 468 97 505 617 458 166 1073
Cuts at: 3528 4461 6819
Size: 933 2358
Fragments arranged by size:
2358 1073 933 617 505 468 458 166
144 97
SfoI GGC'GCC
Cuts at: 0 3971 6438 6819
Size: 3971 2467 381
Fragments arranged by size:
3971 2467 381
SmlI C'TyrA_G
Cuts at: 0 551 794 3285 5811 6469 6785 6819
Size: 551 243 2491 2526 658 316 34
Fragments arranged by size:
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SphI G_CATG'C
Cuts at: 0 3198 4876 6324 6819
Size: 3198 1678 1448 495
Fragments arranged by size:
3198 1678 1448 495
SspI AAT'ATT
Cuts at: 0 827 6018 6819
Size: 827 5191 801
Fragments arranged by size:
5191 827 801
StuI AGG'CCT
Cuts at: 0 1039 2447 3149 4792 6819
Size: 1039 1408 702 1643 2027
Fragments arranged by size:
2027 1643 1408 1039 702
StyI C'CwwG_G
Cuts at: 0 363 2267 2608 3012 3239 3375 3473 3768
Size: 363 1904 341 404 227 136 98 295
Cuts at: 3768 4746 4991 5642 6819
Size: 978 245 651 1177
Fragments arranged by size:
1904 1177 978 651 404 363 341 295
245 227 136 98
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 882 1618 2704 2741 3998 4426 5160 6819
Size: 882 736 1086 37 1257 428 734 1659
Fragments arranged by size:
1659 1257 1086 882 736 734 428 37
TatI w'GTAC_w
Cuts at: 0 1196 3464 4238 5663 6698 6819
Size: 1196 2268 774 1425 1035 121
Fragments arranged by size:
2268 1425 1196 1035 774 121
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 73 105 379 411 874 906 3501 3533
Size: 73 32 274 32 463 32 2595 32
Cuts at: 3533 3546 3578 4034 4066 5857 5889 6819
Size: 13 32 456 32 1791 32 930
Fragments arranged by size:
2595 1791 930 463 456 274 73 32
32 32 32 32 32 32 13
XbaI T'CTAG_A
Cuts at: 0 4824 6819
Size: 4824 1995
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2274 2896 3225 3270 3354 5143 5859 6297
Size: 2274 622 329 45 84 1789 716 438
Cuts at: 6297 6819
Size: 522
Fragments arranged by size:
2274 1789 716 622 522 438 329 84
45
XhoI C'TCGA_G
Cuts at: 0 3285 6785 6819
Size: 3285 3500 34
Fragments arranged by size:
3500 3285 34
XmnI GAAnn'nnTTC
Cuts at: 0 5276 6819
Size: 5276 1543
ZraI GAC'GTC
Cuts at: 0 4296 6819
Size: 4296 2523
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AhdI
AleI AloI AlwNI ApaI ApoI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PasI
PciI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SmlI SphI SspI StuI StyI
TaqII TatI TstI XbaI XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AclI AflII AgeI AlfI ApaLI AscI AseI AsiSI
AvrII BaeI BmtI BsiWI BssHII BstBI BstZ17I DraI
EcoICRI FseI FspAI HindIII HpaI MfeI MluI NheI
NotI NruI PacI PmeI PpiI PsrI PvuI RsrII
SacI SacII SfiI SgfI SgrAI SmaI SnaBI SpeI
SrfI SwaI Tth111I XmaI