(Circular) (Six-base) MAPSORT of: pFN21ASDB0050.seq Check: 6852 from: 1 to: 8599 pFN21ASDB0050 8599 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3934 3934 Size: 8599 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6409 278 Size: 53 83 186 936 4873 2468 Fragments arranged by size: 4873 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4896 5942 1937 Size: 2959 1046 4594 Fragments arranged by size: 4594 2959 1046 Acc65I G'GTAC_C Cuts at: 4026 5920 4026 Size: 1894 6705 AclI AA'CG_TT Cuts at: 1563 6726 7099 1563 Size: 5163 373 3063 Fragments arranged by size: 5163 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2573 4857 5009 5591 5674 6662 8046 8314 Size: 941 2284 152 582 83 988 1384 268 Cuts at: 8314 1632 Size: 1917 Fragments arranged by size: 2284 1917 1384 988 941 582 268 152 83 AflII C'TTAA_G Cuts at: 820 1017 3386 820 Size: 197 2369 6033 Fragments arranged by size: 6033 2369 197 AflIII A'CryG_T Cuts at: 1203 2415 3030 4959 5517 7498 8475 1203 Size: 1212 615 1929 558 1981 977 1327 Fragments arranged by size: 1981 1929 1327 1212 977 615 558 AgeI A'CCGG_T Cuts at: 7430 7430 Size: 8599 AhdI GACnn_n'nnGTC Cuts at: 7328 7328 Size: 8599 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8599 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2982 3668 1184 Size: 1798 686 6115 Fragments arranged by size: 6115 1798 686 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2549 2581 2549 Size: 32 8567 AlwNI CAG_nnn'CTG Cuts at: 3989 5074 5199 6342 7914 3989 Size: 1085 125 1143 1572 4674 Fragments arranged by size: 4674 1572 1143 1085 125 ApaI G_GGCC'C Cuts at: 2064 4342 5314 5857 2064 Size: 2278 972 543 4806 Fragments arranged by size: 4806 2278 972 543 ApaLI G'TGCA_C Cuts at: 6656 7812 6656 Size: 1156 7443 ApoI r'AATT_y Cuts at: 1493 2363 5480 5533 5908 6134 6286 8395 1493 Size: 870 3117 53 375 226 152 2109 1697 Fragments arranged by size: 3117 2109 1697 870 375 226 152 53 AscI GG'CGCG_CC Cuts at: 2067 2067 Size: 8599 AseI AT'TA_AT Cuts at: 160 1032 7153 160 Size: 872 6121 1606 Fragments arranged by size: 6121 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8599 AvaI C'yCGr_G Cuts at: 1870 1943 2108 2159 5754 5868 5924 1870 Size: 73 165 51 3595 114 56 4545 Fragments arranged by size: 4545 3595 165 114 73 56 51 AvrII C'CTAG_G Cuts at: 4624 4624 Size: 8599 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3832 3865 3832 Size: 33 8566 BamHI G'GATC_C Cuts at: 5929 6296 5929 Size: 367 8232 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2030 2117 2216 2461 3445 Size: 325 217 627 243 87 99 245 984 Cuts at: 3445 3946 4026 5920 7375 618 Size: 501 80 1894 1455 1842 Fragments arranged by size: 1894 1842 1455 984 627 501 325 245 243 217 99 87 80 BanII G_rGCy'C Cuts at: 1391 2064 3369 3627 3757 4342 4881 5314 5720 Size: 673 1305 258 130 585 539 433 406 Cuts at: 5720 5755 5857 5918 1391 Size: 35 102 61 4072 Fragments arranged by size: 4072 1305 673 585 539 433 406 258 130 102 61 35 BbeI G_GCGC'C Cuts at: 2034 2220 2034 Size: 186 8413 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2174 2282 2705 4702 5408 5520 5579 Size: 971 275 108 423 1997 706 112 59 Cuts at: 5579 928 Size: 3948 Fragments arranged by size: 3948 1997 971 706 423 275 112 108 59 BbvCI CC'TCA_GC Cuts at: 3423 4146 3423 Size: 723 7876 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6790 6824 1903 Size: 34 4853 34 3678 Fragments arranged by size: 4853 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2443 3549 3860 5573 6491 7701 2443 Size: 1106 311 1713 918 1210 3341 Fragments arranged by size: 3341 1713 1210 1106 918 311 BclI T'GATC_A Cuts at: 1548 5640 1548 Size: 4092 4507 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3230 7210 136 Size: 107 122 71 631 605 1558 3980 1525 Fragments arranged by size: 3980 1558 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3351 3570 8594 3351 Size: 219 5024 3356 Fragments arranged by size: 5024 3356 219 BlpI GC'TnA_GC Cuts at: 2381 2664 2855 3717 4056 4323 6016 2381 Size: 283 191 862 339 267 1693 4964 Fragments arranged by size: 4964 1693 862 339 283 267 191 Bme1580I G_kGCm'C Cuts at: 1790 2064 2120 3168 3951 4342 5314 5857 6660 Size: 274 56 1048 783 391 972 543 803 Cuts at: 6660 7816 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1048 972 803 783 543 391 274 56 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8599 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3239 4795 5552 Size: 416 53 83 399 24 1822 1556 757 Cuts at: 5552 6330 7288 442 Size: 778 958 1753 Fragments arranged by size: 1822 1753 1556 958 778 757 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8599 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3673 5647 5734 3673 Size: 1974 87 6538 Fragments arranged by size: 6538 1974 87 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2207 2645 2819 3040 3533 4325 4607 4895 Size: 968 438 174 221 493 792 282 288 Cuts at: 4895 5297 5394 5642 7259 1239 Size: 402 97 248 1617 2579 Fragments arranged by size: 2579 1617 968 792 493 438 402 288 282 248 221 174 97 Bpu10I CC'TnA_GC Cuts at: 3423 4146 5544 3423 Size: 723 1398 6478 Fragments arranged by size: 6478 1398 723 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6724 7589 7887 6724 Size: 865 298 7436 Fragments arranged by size: 7436 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2862 3581 3740 3782 7262 882 Size: 617 1363 719 159 42 3480 2219 Fragments arranged by size: 3480 2219 1363 719 617 159 42 BsaAI yAC'GTr Cuts at: 493 1204 2205 3033 4558 4996 5209 5896 6390 Size: 711 1001 828 1525 438 213 687 494 Cuts at: 6390 493 Size: 2702 Fragments arranged by size: 2702 1525 1001 828 711 687 494 438 213 BsaBI GATnn'nnATC Cuts at: 2298 2520 3401 4673 8593 2298 Size: 222 881 1272 3920 2304 Fragments arranged by size: 3920 2304 1272 881 222 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2031 2079 2217 3706 Size: 53 83 186 936 498 48 138 1489 Cuts at: 3706 4727 4824 6406 6788 7423 275 Size: 1021 97 1582 382 635 1451 Fragments arranged by size: 1582 1489 1451 1021 936 635 498 382 186 138 97 83 53 48 BsaWI w'CCGG_w Cuts at: 2493 3749 7032 7430 7704 7851 8262 2493 Size: 1256 3283 398 274 147 411 2830 Fragments arranged by size: 3283 2830 1256 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2999 3029 3038 3068 1178 Size: 30 165 30 1596 30 9 30 6709 Fragments arranged by size: 6709 1596 165 30 30 30 30 9 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2903 3007 3010 3323 3455 3599 3632 3635 Size: 1717 104 3 313 132 144 33 3 Cuts at: 3635 4310 4787 1186 Size: 675 477 4998 Fragments arranged by size: 4998 1717 675 477 313 144 132 104 33 3 3 BseYI C'CCAG_C Cuts at: 2311 2741 3126 3282 3319 3869 5305 7802 8298 Size: 430 385 156 37 550 1436 2497 496 Cuts at: 8298 2311 Size: 2612 Fragments arranged by size: 2612 2497 1436 550 496 430 385 156 37 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2075 2861 3188 3638 4425 1163 Size: 573 339 786 327 450 787 5337 Fragments arranged by size: 5337 787 786 573 450 339 327 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2324 2753 6049 6810 7838 1540 Size: 275 186 323 429 3296 761 1028 2301 Fragments arranged by size: 3296 2301 1028 761 429 323 275 186 BsiHKAI G_wGCw'C Cuts at: 4051 4062 4605 4881 6660 6745 7816 4051 Size: 11 543 276 1779 85 1071 4834 Fragments arranged by size: 4834 1779 1071 543 276 85 11 BsmI GAATG_Cn' Cuts at: 6110 6203 6110 Size: 93 8506 BsmBI CGTCTCn'nnnn_ Cuts at: 2183 2183 Size: 8599 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2064 2120 3168 3369 3627 3757 3951 Size: 399 274 56 1048 201 258 130 194 Cuts at: 3951 4051 4062 4342 4605 4881 5314 5720 5755 Size: 100 11 280 263 276 433 406 35 Cuts at: 5755 5857 5918 6660 6745 7816 1391 Size: 102 61 742 85 1071 2174 Fragments arranged by size: 2174 1071 1048 742 433 406 399 280 276 274 263 258 201 194 130 102 100 85 61 56 35 11 BspEI T'CCGG_A Cuts at: 2493 8262 2493 Size: 5769 2830 BspHI T'CATG_A Cuts at: 2124 5184 6488 8218 2124 Size: 3060 1304 1730 2505 Fragments arranged by size: 3060 2505 1730 1304 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3934 5954 844 Size: 3090 2020 3489 Fragments arranged by size: 3489 3090 2020 BsrBI CCG'CTC Cuts at: 1394 1823 2164 2611 2749 5713 6486 1394 Size: 429 341 447 138 2964 773 3507 Fragments arranged by size: 3507 2964 773 447 429 341 138 BsrDI GCAATG_nn' Cuts at: 65 1248 2335 2453 3276 4162 7094 7268 65 Size: 1183 1087 118 823 886 2932 174 1396 Fragments arranged by size: 2932 1396 1183 1087 886 823 174 118 BsrFI r'CCGG_y Cuts at: 1700 1810 4657 4698 4902 7243 7430 1700 Size: 110 2847 41 204 2341 187 2869 Fragments arranged by size: 2869 2847 2341 204 187 110 41 BsrGI T'GTAC_A Cuts at: 96 2685 96 Size: 2589 6010 BssHII G'CGCG_C Cuts at: 1925 2067 1925 Size: 142 8457 BssSI C'ACGA_G Cuts at: 3773 4010 4080 6659 7671 3773 Size: 237 70 2579 1012 4701 Fragments arranged by size: 4701 2579 1012 237 70 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8599 BstBI TT'CG_AA Cuts at: 2007 7403 2007 Size: 5396 3203 BstEII G'GTnAC_C Cuts at: 1184 5675 1184 Size: 4491 4108 BstXI CCAn_nnnn'nTGG Cuts at: 3346 4723 3346 Size: 1377 7222 BstYI r'GATC_y Cuts at: 1972 2262 3012 3351 3396 3570 4797 5109 5929 Size: 290 750 339 45 174 1227 312 820 Cuts at: 5929 6296 6681 6698 8150 8236 8248 8594 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1227 820 750 385 367 346 339 312 290 174 86 45 17 12 Bsu36I CC'TnA_GG Cuts at: 4683 5728 4683 Size: 1045 7554 BtgI C'CryG_G Cuts at: 513 1065 2022 3160 3345 3676 4578 5269 513 Size: 552 957 1138 185 331 902 691 3843 Fragments arranged by size: 3843 1138 957 902 691 552 331 185 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3697 487 Size: 683 56 2471 5389 Fragments arranged by size: 5389 2471 683 56 BtsI GCAGTG_nn' Cuts at: 3268 3436 3601 3841 5595 6115 6909 6929 3268 Size: 168 165 240 1754 520 794 20 4938 Fragments arranged by size: 4938 1754 794 520 240 168 165 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8599 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4539 4574 529 Size: 35 3975 35 4554 Fragments arranged by size: 4554 3975 35 35 DraI TTT'AAA Cuts at: 5903 5903 Size: 8599 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3902 7606 809 Size: 507 2586 3704 1802 Fragments arranged by size: 3704 2586 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2321 2750 5332 Size: 54 1425 50 275 18 491 429 2582 Cuts at: 5332 5441 5746 6046 6935 8564 8 Size: 109 305 300 889 1629 43 Fragments arranged by size: 2582 1629 1425 889 491 429 305 300 275 109 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2321 2750 6046 1537 Size: 275 509 429 3296 4090 Fragments arranged by size: 4090 3296 509 429 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4557 4709 4917 5186 6529 1353 Size: 3204 152 208 269 1343 3423 Fragments arranged by size: 3423 3204 1343 269 208 152 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2255 3838 7186 7560 7706 387 Size: 1064 138 666 1583 3348 374 146 1280 Fragments arranged by size: 3348 1583 1280 1064 666 374 146 138 EcoICRI GAG'CTC Cuts at: 4879 4879 Size: 8599 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2207 2573 2645 2819 3040 3533 4325 Size: 393 575 366 72 174 221 493 792 Cuts at: 4325 4607 4857 4895 5009 5297 5394 5591 5642 Size: 282 250 38 114 288 97 197 51 Cuts at: 5642 5674 6662 7259 8046 8314 1239 Size: 32 988 597 787 268 1524 Fragments arranged by size: 1524 988 792 787 597 575 493 393 366 288 282 268 250 221 197 174 114 97 72 51 38 32 EcoNI CCTnn'n_nnAGG Cuts at: 4346 5543 4346 Size: 1197 7402 EcoO109I rG'GnC_Cy Cuts at: 2308 2548 2584 3517 3873 4089 4338 4339 5176 Size: 240 36 933 356 216 249 1 837 Cuts at: 5176 5310 5617 5853 5854 2308 Size: 134 307 236 1 5053 Fragments arranged by size: 5053 933 837 356 307 249 240 236 216 134 36 1 1 EcoRI G'AATT_C Cuts at: 5908 6286 5908 Size: 378 8221 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3904 3904 Size: 8599 FspI TGC'GCA Cuts at: 7105 7105 Size: 8599 HaeII r_GCGC'y Cuts at: 1678 2034 2220 2694 3724 4470 7746 1678 Size: 356 186 474 1030 746 3276 2531 Fragments arranged by size: 3276 2531 1030 746 474 356 186 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2522 2554 3038 3070 3088 Size: 32 135 32 950 32 484 32 18 Cuts at: 3088 3120 4949 4981 5028 5060 7240 7272 7314 Size: 32 1829 32 47 32 2180 32 42 Cuts at: 7314 7346 1373 Size: 32 2626 Fragments arranged by size: 2626 2180 1829 950 484 135 47 42 32 32 32 32 32 32 32 32 32 18 HincII GTy'rAC Cuts at: 669 1657 1938 2575 4228 4573 5943 6189 669 Size: 988 281 637 1653 345 1370 246 3079 Fragments arranged by size: 3079 1653 1370 988 637 345 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8599 HpaI GTT'AAC Cuts at: 6189 6189 Size: 8599 KasI G'GCGC_C Cuts at: 2030 2216 2030 Size: 186 8413 KpnI G_GTAC'C Cuts at: 4030 5924 4030 Size: 1894 6705 MfeI C'AATT_G Cuts at: 6198 6198 Size: 8599 MluI A'CGCG_T Cuts at: 8475 8475 Size: 8599 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3099 5109 7713 7897 1305 Size: 1794 2010 2604 184 2007 Fragments arranged by size: 2604 2010 2007 1794 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 5443 10 Size: 54 1425 343 3611 3166 Fragments arranged by size: 3611 3166 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2123 3660 4067 4167 4721 6557 6916 Size: 648 957 1537 407 100 554 1836 359 Cuts at: 6916 7075 518 Size: 159 2042 Fragments arranged by size: 2042 1836 1537 957 648 554 407 359 159 100 MspA1I CmG'CkG Cuts at: 1606 2637 2668 2962 4322 4537 5271 5737 6015 Size: 1031 31 294 1360 215 734 466 278 Cuts at: 6015 6692 7840 8085 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1360 1148 1031 734 677 466 294 278 245 215 31 NaeI GCC'GGC Cuts at: 4659 4904 4659 Size: 245 8354 NarI GG'CG_CC Cuts at: 2031 2217 2031 Size: 186 8413 NcoI C'CATG_G Cuts at: 513 1065 2022 3160 3676 513 Size: 552 957 1138 516 5436 Fragments arranged by size: 5436 1138 957 552 516 NdeI CA'TA_TG Cuts at: 387 4126 387 Size: 3739 4860 NgoMIV G'CCGG_C Cuts at: 4657 4902 4657 Size: 245 8354 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8599 NotI GC'GGCC_GC Cuts at: 6046 6046 Size: 8599 NspI r_CATG'y Cuts at: 1132 1229 2419 2563 2596 3224 4066 4963 5521 Size: 97 1190 144 33 628 842 897 558 Cuts at: 5521 5957 7502 1132 Size: 436 1545 2229 Fragments arranged by size: 2229 1545 1190 897 842 628 558 436 144 97 33 PasI CC'CwG_GG Cuts at: 1348 2270 1348 Size: 922 7677 PciI A'CATG_T Cuts at: 2415 4959 5517 7498 2415 Size: 2544 558 1981 3516 Fragments arranged by size: 3516 2544 1981 558 PflMI CCAn_nnn'nTGG Cuts at: 1108 4483 5074 5330 1108 Size: 3375 591 256 4377 Fragments arranged by size: 4377 3375 591 256 PfoI T'CCnGG_A Cuts at: 2904 4971 2904 Size: 2067 6532 PmeI GTTT'AAAC Cuts at: 5903 5903 Size: 8599 PmlI CAC'GTG Cuts at: 2205 2205 Size: 8599 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3656 3688 6668 6700 8181 8213 3656 Size: 32 2980 32 1481 32 4042 Fragments arranged by size: 4042 2980 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2308 2548 2584 3517 3873 5617 2308 Size: 240 36 933 356 1744 5290 Fragments arranged by size: 5290 1744 933 356 240 36 PshAI GACnn'nnGTC Cuts at: 1613 3705 5682 5870 1613 Size: 2092 1977 188 4342 Fragments arranged by size: 4342 2092 1977 188 PsiI TTA'TAA Cuts at: 6169 6169 Size: 8599 PspOMI G'GGCC_C Cuts at: 2060 4338 5310 5853 2060 Size: 2278 972 543 4806 Fragments arranged by size: 4806 2278 972 543 PspXI vC'TCGA_Gb Cuts at: 2108 2108 Size: 8599 PstI C_TGCA'G Cuts at: 830 2478 3275 4782 5043 5217 5951 830 Size: 1648 797 1507 261 174 734 3478 Fragments arranged by size: 3478 1648 1507 797 734 261 174 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8599 PvuII CAG'CTG Cuts at: 2668 2962 4322 4537 5737 2668 Size: 294 1360 215 1200 5530 Fragments arranged by size: 5530 1360 1200 294 215 SacI G_AGCT'C Cuts at: 4881 4881 Size: 8599 SacII CC_GC'GG Cuts at: 5272 5272 Size: 8599 SalI G'TCGA_C Cuts at: 1936 5941 1936 Size: 4005 4594 SanDI GG'GwC_CC Cuts at: 2584 3873 2584 Size: 1289 7310 SapI GCTCTTCn'nnn_ Cuts at: 4709 4709 Size: 8599 SbfI CC_TGCA'GG Cuts at: 5951 5951 Size: 8599 ScaI AGT'ACT Cuts at: 2731 3737 6847 8559 2731 Size: 1006 3110 1712 2771 Fragments arranged by size: 3110 2771 1712 1006 SfcI C'TryA_G Cuts at: 826 1045 2474 3271 4778 5039 5213 5608 5947 Size: 219 1429 797 1507 261 174 395 339 Cuts at: 5947 7082 7763 7954 826 Size: 1135 681 191 1471 Fragments arranged by size: 1507 1471 1429 1135 797 681 395 339 261 219 191 174 SfoI GGC'GCC Cuts at: 2032 2218 2032 Size: 186 8413 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8599 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8599 SmaI CCC'GGG Cuts at: 1872 5756 5926 1872 Size: 3884 170 4545 Fragments arranged by size: 4545 3884 170 SmlI C'TyrA_G Cuts at: 820 1017 1943 2108 3386 6703 7604 7866 820 Size: 197 926 165 1278 3317 901 262 1553 Fragments arranged by size: 3317 1553 1278 926 901 262 197 165 SnaBI TAC'GTA Cuts at: 493 5896 6390 493 Size: 5403 494 2702 Fragments arranged by size: 5403 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8599 SphI G_CATG'C Cuts at: 1132 3224 5957 1132 Size: 2092 2733 3774 Fragments arranged by size: 3774 2733 2092 SspI AAT'ATT Cuts at: 5 52 6523 5 Size: 47 6471 2081 Fragments arranged by size: 6471 2081 47 StuI AGG'CCT Cuts at: 1517 5240 5568 1517 Size: 3723 328 4548 Fragments arranged by size: 4548 3723 328 StyI C'CwwG_G Cuts at: 513 1065 2022 2544 3069 3160 3627 3676 4624 Size: 552 957 522 525 91 467 49 948 Cuts at: 4624 4980 5061 5664 6038 513 Size: 356 81 603 374 3074 Fragments arranged by size: 3074 957 948 603 552 525 522 467 374 356 91 81 49 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2479 3391 4777 6626 6643 6796 6981 Size: 1255 899 912 1386 1849 17 153 185 Cuts at: 6981 7426 325 Size: 445 1498 Fragments arranged by size: 1849 1498 1386 1255 912 899 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2685 2729 3462 Size: 274 80 33 51 1443 708 44 733 Cuts at: 3462 3735 5028 6845 8557 96 Size: 273 1293 1817 1712 138 Fragments arranged by size: 1817 1712 1443 1293 733 708 274 273 138 80 51 44 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8559 8591 8559 Size: 32 8567 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8599 XbaI T'CTAG_A Cuts at: 5935 5935 Size: 8599 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5741 5741 Size: 8599 XhoI C'TCGA_G Cuts at: 1943 2108 1943 Size: 165 8434 XmaI C'CCGG_G Cuts at: 1870 5754 5924 1870 Size: 3884 170 4545 Fragments arranged by size: 4545 3884 170 XmnI GAAnn'nnTTC Cuts at: 2171 3671 6728 2171 Size: 1500 3057 4042 Fragments arranged by size: 4042 3057 1500 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6407 276 Size: 53 83 186 936 4873 2468 Fragments arranged by size: 4873 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI BfrBI BsiWI BstZ17I DraIII EcoRV FseI FspAI NruI NsiI PacI PsrI RsrII SexAI SfiI SrfI SwaI