Restriction map for
pFN21ASDB0050
(Linear) (Six-base) MAPSORT of: KIBB0050.seq Check: 4926 from: 1 to: 3870
KIBB0050.orf
With 182 enzymes: SgfI *
February 28, 2007 11:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1911 3870
Size: 1911 1959
AccI GT'mk_AC
Cuts at: 0 2873 3870
Size: 2873 997
Acc65I G'GTAC_C
Cuts at: 0 2003 3870
Size: 2003 1867
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 550 2834 2986 3568 3651 3870
Size: 550 2284 152 582 83 219
Fragments arranged by size:
2284 582 550 219 152 83
AflII C'TTAA_G
Cuts at: 0 1363 3870
Size: 1363 2507
AflIII A'CryG_T
Cuts at: 0 392 1007 2936 3494 3870
Size: 392 615 1929 558 376
Fragments arranged by size:
1929 615 558 392 376
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 959 1645 3870
Size: 959 686 2225
Fragments arranged by size:
2225 959 686
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 526 558 3870
Size: 526 32 3312
Fragments arranged by size:
3312 526 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1966 3051 3176 3870
Size: 1966 1085 125 694
Fragments arranged by size:
1966 1085 694 125
ApaI G_GGCC'C
Cuts at: 0 41 2319 3291 3834 3870
Size: 41 2278 972 543 36
Fragments arranged by size:
2278 972 543 41 36
ApoI r'AATT_y
Cuts at: 0 340 3457 3510 3870
Size: 340 3117 53 360
Fragments arranged by size:
3117 360 340 53
AscI GG'CGCG_CC
Cuts at: 0 44 3870
Size: 44 3826
AvaI C'yCGr_G
Cuts at: 0 85 136 3731 3845 3870
Size: 85 51 3595 114 25
Fragments arranged by size:
3595 114 85 51 25
AvrII C'CTAG_G
Cuts at: 0 2601 3870
Size: 2601 1269
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1809 1842 3870
Size: 1809 33 2028
Fragments arranged by size:
2028 1809 33
BanI G'GyrC_C
Cuts at: 0 7 94 193 438 1422 1923 2003 3870
Size: 7 87 99 245 984 501 80 1867
Fragments arranged by size:
1867 984 501 245 99 87 80 7
BanII G_rGCy'C
Cuts at: 0 41 1346 1604 1734 2319 2858 3291 3697
Size: 41 1305 258 130 585 539 433 406
Cuts at: 3697 3732 3834 3870
Size: 35 102 36
Fragments arranged by size:
1305 585 539 433 406 258 130 102
41 36 35
BbeI G_GCGC'C
Cuts at: 0 11 197 3870
Size: 11 186 3673
Fragments arranged by size:
3673 186 11
BbsI GAAGACnn'nnnn_
Cuts at: 0 151 259 682 2679 3385 3497 3556 3870
Size: 151 108 423 1997 706 112 59 314
Fragments arranged by size:
1997 706 423 314 151 112 108 59
BbvCI CC'TCA_GC
Cuts at: 0 1400 2123 3870
Size: 1400 723 1747
Fragments arranged by size:
1747 1400 723
BciVI GTATCCnnnnn_n'
Cuts at: 0 420 1526 1837 3550 3870
Size: 420 1106 311 1713 320
Fragments arranged by size:
1713 1106 420 320 311
BclI T'GATC_A
Cuts at: 0 3617 3870
Size: 3617 253
BglI GCCn_nnn'nGGC
Cuts at: 0 1207 3870
Size: 1207 2663
BglII A'GATC_T
Cuts at: 0 1328 1547 3870
Size: 1328 219 2323
Fragments arranged by size:
2323 1328 219
BlpI GC'TnA_GC
Cuts at: 0 358 641 832 1694 2033 2300 3870
Size: 358 283 191 862 339 267 1570
Fragments arranged by size:
1570 862 358 339 283 267 191
Bme1580I G_kGCm'C
Cuts at: 0 41 97 1145 1928 2319 3291 3834 3870
Size: 41 56 1048 783 391 972 543 36
Fragments arranged by size:
1048 972 783 543 391 56 41 36
BmrI ACTGGGnnnn_n'
Cuts at: 0 1216 2772 3529 3870
Size: 1216 1556 757 341
Fragments arranged by size:
1556 1216 757 341
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1650 3624 3711 3870
Size: 1650 1974 87 159
Fragments arranged by size:
1974 1650 159 87
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 622 796 1017 1510 2302 2584 2872
Size: 184 438 174 221 493 792 282 288
Cuts at: 2872 3274 3371 3619 3870
Size: 402 97 248 251
Fragments arranged by size:
792 493 438 402 288 282 251 248
221 184 174 97
Bpu10I CC'TnA_GC
Cuts at: 0 1400 2123 3521 3870
Size: 1400 723 1398 349
Fragments arranged by size:
1400 1398 723 349
BsaI GGTCTCn'nnnn_
Cuts at: 0 839 1558 1717 1759 3870
Size: 839 719 159 42 2111
Fragments arranged by size:
2111 839 719 159 42
BsaAI yAC'GTr
Cuts at: 0 182 1010 2535 2973 3186 3870
Size: 182 828 1525 438 213 684
Fragments arranged by size:
1525 828 684 438 213 182
BsaBI GATnn'nnATC
Cuts at: 0 275 497 1378 2650 3870
Size: 275 222 881 1272 1220
Fragments arranged by size:
1272 1220 881 275 222
BsaHI Gr'CG_yC
Cuts at: 0 8 56 194 1683 2704 2801 3870
Size: 8 48 138 1489 1021 97 1069
Fragments arranged by size:
1489 1069 1021 138 97 48 8
BsaWI w'CCGG_w
Cuts at: 0 470 1726 3870
Size: 470 1256 2144
Fragments arranged by size:
2144 1256 470
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 976 1006 1015 1045 3870
Size: 976 30 9 30 2825
Fragments arranged by size:
2825 976 30 30 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 880 984 987 1300 1432 1576 1609 1612
Size: 880 104 3 313 132 144 33 3
Cuts at: 1612 2287 2764 3870
Size: 675 477 1106
Fragments arranged by size:
1106 880 675 477 313 144 132 104
33 3 3
BseYI C'CCAG_C
Cuts at: 0 288 718 1103 1259 1296 1846 3282 3870
Size: 288 430 385 156 37 550 1436 588
Fragments arranged by size:
1436 588 550 430 385 288 156 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 838 1165 1615 2402 3870
Size: 52 786 327 450 787 1468
Fragments arranged by size:
1468 787 786 450 327 52
BsiEI CG_ry'CG
Cuts at: 0 301 730 3870
Size: 301 429 3140
Fragments arranged by size:
3140 429 301
BsiHKAI G_wGCw'C
Cuts at: 0 2028 2039 2582 2858 3870
Size: 2028 11 543 276 1012
Fragments arranged by size:
2028 1012 543 276 11
BsmBI CGTCTCn'nnnn_
Cuts at: 0 160 3870
Size: 160 3710
Bsp1286I G_dGCh'C
Cuts at: 0 41 97 1145 1346 1604 1734 1928 2028
Size: 41 56 1048 201 258 130 194 100
Cuts at: 2028 2039 2319 2582 2858 3291 3697 3732 3834
Size: 11 280 263 276 433 406 35 102
Cuts at: 3834 3870
Size: 36
Fragments arranged by size:
1048 433 406 280 276 263 258 201
194 130 102 100 56 41 36 35
11
BspEI T'CCGG_A
Cuts at: 0 470 3870
Size: 470 3400
BspHI T'CATG_A
Cuts at: 0 101 3161 3870
Size: 101 3060 709
Fragments arranged by size:
3060 709 101
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1911 3870
Size: 1911 1959
BsrBI CCG'CTC
Cuts at: 0 141 588 726 3690 3870
Size: 141 447 138 2964 180
Fragments arranged by size:
2964 447 180 141 138
BsrDI GCAATG_nn'
Cuts at: 0 312 430 1253 2139 3870
Size: 312 118 823 886 1731
Fragments arranged by size:
1731 886 823 312 118
BsrFI r'CCGG_y
Cuts at: 0 2634 2675 2879 3870
Size: 2634 41 204 991
Fragments arranged by size:
2634 991 204 41
BsrGI T'GTAC_A
Cuts at: 0 662 3870
Size: 662 3208
BssHII G'CGCG_C
Cuts at: 0 44 3870
Size: 44 3826
BssSI C'ACGA_G
Cuts at: 0 1750 1987 2057 3870
Size: 1750 237 70 1813
Fragments arranged by size:
1813 1750 237 70
BstEII G'GTnAC_C
Cuts at: 0 3652 3870
Size: 3652 218
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1323 2700 3870
Size: 1323 1377 1170
Fragments arranged by size:
1377 1323 1170
BstYI r'GATC_y
Cuts at: 0 239 989 1328 1373 1547 2774 3086 3870
Size: 239 750 339 45 174 1227 312 784
Fragments arranged by size:
1227 784 750 339 312 239 174 45
Bsu36I CC'TnA_GG
Cuts at: 0 2660 3705 3870
Size: 2660 1045 165
Fragments arranged by size:
2660 1045 165
BtgI C'CryG_G
Cuts at: 0 1137 1322 1653 2555 3246 3870
Size: 1137 185 331 902 691 624
Fragments arranged by size:
1137 902 691 624 331 185
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1674 3870
Size: 1674 2196
BtsI GCAGTG_nn'
Cuts at: 0 1245 1413 1578 1818 3572 3870
Size: 1245 168 165 240 1754 298
Fragments arranged by size:
1754 1245 298 240 168 165
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2516 2551 3870
Size: 2516 35 1319
Fragments arranged by size:
2516 1319 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1879 3870
Size: 1879 1991
EaeI y'GGCC_r
Cuts at: 0 298 727 3309 3418 3723 3870
Size: 298 429 2582 109 305 147
Fragments arranged by size:
2582 429 305 298 147 109
EagI C'GGCC_G
Cuts at: 0 298 727 3870
Size: 298 429 3143
Fragments arranged by size:
3143 429 298
EarI CTCTTCn'nnn_
Cuts at: 0 2534 2686 2894 3163 3870
Size: 2534 152 208 269 707
Fragments arranged by size:
2534 707 269 208 152
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 232 1815 3870
Size: 232 1583 2055
Fragments arranged by size:
2055 1583 232
EcoICRI GAG'CTC
Cuts at: 0 2856 3870
Size: 2856 1014
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 550 622 796 1017 1510 2302 2584
Size: 184 366 72 174 221 493 792 282
Cuts at: 2584 2834 2872 2986 3274 3371 3568 3619 3651
Size: 250 38 114 288 97 197 51 32
Cuts at: 3651 3870
Size: 219
Fragments arranged by size:
792 493 366 288 282 250 221 219
197 184 174 114 97 72 51 38
32
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2323 3520 3870
Size: 2323 1197 350
Fragments arranged by size:
2323 1197 350
EcoO109I rG'GnC_Cy
Cuts at: 0 285 525 561 1494 1850 2066 2315 2316
Size: 285 240 36 933 356 216 249 1
Cuts at: 2316 3153 3287 3594 3830 3831 3870
Size: 837 134 307 236 1 39
Fragments arranged by size:
933 837 356 307 285 249 240 236
216 134 39 36 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1881 3870
Size: 1881 1989
HaeII r_GCGC'y
Cuts at: 0 11 197 671 1701 2447 3870
Size: 11 186 474 1030 746 1423
Fragments arranged by size:
1423 1030 746 474 186 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 499 531 1015 1047 1065 1097 2926 2958
Size: 499 32 484 32 18 32 1829 32
Cuts at: 2958 3005 3037 3870
Size: 47 32 833
Fragments arranged by size:
1829 833 499 484 47 32 32 32
32 32 18
HincII GTy'rAC
Cuts at: 0 552 2205 2550 3870
Size: 552 1653 345 1320
Fragments arranged by size:
1653 1320 552 345
KasI G'GCGC_C
Cuts at: 0 7 193 3870
Size: 7 186 3677
Fragments arranged by size:
3677 186 7
KpnI G_GTAC'C
Cuts at: 0 2007 3870
Size: 2007 1863
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1076 3086 3870
Size: 1076 2010 784
Fragments arranged by size:
2010 1076 784
MscI TGG'CCA
Cuts at: 0 3420 3870
Size: 3420 450
MslI CAynn'nnrTG
Cuts at: 0 100 1637 2044 2144 2698 3870
Size: 100 1537 407 100 554 1172
Fragments arranged by size:
1537 1172 554 407 100 100
MspA1I CmG'CkG
Cuts at: 0 614 645 939 2299 2514 3248 3714 3870
Size: 614 31 294 1360 215 734 466 156
Fragments arranged by size:
1360 734 614 466 294 215 156 31
NaeI GCC'GGC
Cuts at: 0 2636 2881 3870
Size: 2636 245 989
Fragments arranged by size:
2636 989 245
NarI GG'CG_CC
Cuts at: 0 8 194 3870
Size: 8 186 3676
Fragments arranged by size:
3676 186 8
NcoI C'CATG_G
Cuts at: 0 1137 1653 3870
Size: 1137 516 2217
Fragments arranged by size:
2217 1137 516
NdeI CA'TA_TG
Cuts at: 0 2103 3870
Size: 2103 1767
NgoMIV G'CCGG_C
Cuts at: 0 2634 2879 3870
Size: 2634 245 991
Fragments arranged by size:
2634 991 245
NspI r_CATG'y
Cuts at: 0 396 540 573 1201 2043 2940 3498 3870
Size: 396 144 33 628 842 897 558 372
Fragments arranged by size:
897 842 628 558 396 372 144 33
PasI CC'CwG_GG
Cuts at: 0 247 3870
Size: 247 3623
PciI A'CATG_T
Cuts at: 0 392 2936 3494 3870
Size: 392 2544 558 376
Fragments arranged by size:
2544 558 392 376
PflMI CCAn_nnn'nTGG
Cuts at: 0 2460 3051 3307 3870
Size: 2460 591 256 563
Fragments arranged by size:
2460 591 563 256
PfoI T'CCnGG_A
Cuts at: 0 881 2948 3870
Size: 881 2067 922
Fragments arranged by size:
2067 922 881
PmlI CAC'GTG
Cuts at: 0 182 3870
Size: 182 3688
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1633 1665 3870
Size: 1633 32 2205
Fragments arranged by size:
2205 1633 32
PpuMI rG'GwC_Cy
Cuts at: 0 285 525 561 1494 1850 3594 3870
Size: 285 240 36 933 356 1744 276
Fragments arranged by size:
1744 933 356 285 276 240 36
PshAI GACnn'nnGTC
Cuts at: 0 1682 3659 3847 3870
Size: 1682 1977 188 23
Fragments arranged by size:
1977 1682 188 23
PspOMI G'GGCC_C
Cuts at: 0 37 2315 3287 3830 3870
Size: 37 2278 972 543 40
Fragments arranged by size:
2278 972 543 40 37
PspXI vC'TCGA_Gb
Cuts at: 0 85 3870
Size: 85 3785
PstI C_TGCA'G
Cuts at: 0 455 1252 2759 3020 3194 3870
Size: 455 797 1507 261 174 676
Fragments arranged by size:
1507 797 676 455 261 174
PvuII CAG'CTG
Cuts at: 0 645 939 2299 2514 3714 3870
Size: 645 294 1360 215 1200 156
Fragments arranged by size:
1360 1200 645 294 215 156
SacI G_AGCT'C
Cuts at: 0 2858 3870
Size: 2858 1012
SacII CC_GC'GG
Cuts at: 0 3249 3870
Size: 3249 621
SanDI GG'GwC_CC
Cuts at: 0 561 1850 3870
Size: 561 1289 2020
Fragments arranged by size:
2020 1289 561
SapI GCTCTTCn'nnn_
Cuts at: 0 2686 3870
Size: 2686 1184
ScaI AGT'ACT
Cuts at: 0 708 1714 3870
Size: 708 1006 2156
Fragments arranged by size:
2156 1006 708
SfcI C'TryA_G
Cuts at: 0 451 1248 2755 3016 3190 3585 3870
Size: 451 797 1507 261 174 395 285
Fragments arranged by size:
1507 797 451 395 285 261 174
SfoI GGC'GCC
Cuts at: 0 9 195 3870
Size: 9 186 3675
Fragments arranged by size:
3675 186 9
SmaI CCC'GGG
Cuts at: 0 3733 3870
Size: 3733 137
SmlI C'TyrA_G
Cuts at: 0 85 1363 3870
Size: 85 1278 2507
Fragments arranged by size:
2507 1278 85
SphI G_CATG'C
Cuts at: 0 1201 3870
Size: 1201 2669
StuI AGG'CCT
Cuts at: 0 3217 3545 3870
Size: 3217 328 325
Fragments arranged by size:
3217 328 325
StyI C'CwwG_G
Cuts at: 0 521 1046 1137 1604 1653 2601 2957 3038
Size: 521 525 91 467 49 948 356 81
Cuts at: 3038 3641 3870
Size: 603 229
Fragments arranged by size:
948 603 525 521 467 356 229 91
81 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 456 1368 2754 3870
Size: 456 912 1386 1116
Fragments arranged by size:
1386 1116 912 456
TatI w'GTAC_w
Cuts at: 0 662 706 1439 1712 3005 3870
Size: 662 44 733 273 1293 865
Fragments arranged by size:
1293 865 733 662 273 44
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3718 3870
Size: 3718 152
XhoI C'TCGA_G
Cuts at: 0 85 3870
Size: 85 3785
XmaI C'CCGG_G
Cuts at: 0 3731 3870
Size: 3731 139
XmnI GAAnn'nnTTC
Cuts at: 0 148 1648 3870
Size: 148 1500 2222
Fragments arranged by size:
2222 1500 148
Enzymes that do cut:
AarI AccI Acc65I AcuI AflII AflIII AlfI AloI
AlwNI ApaI ApoI AscI AvaI AvrII BaeI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
HincII KasI KpnI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PasI PciI PflMI
PfoI PmlI PpiI PpuMI PshAI PspOMI PspXI PstI
PvuII SacI SacII SanDI SapI ScaI SfcI SfoI
SmaI SmlI SphI StuI StyI TaqII TatI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AgeI AhdI AleI ApaLI AseI
AsiSI BamHI BcgI BfrBI BmgBI BmtI BpuEI BsiWI
BsmI BstAPI BstBI BstZ17I ClaI DraI DraIII EcoRI
EcoRV FseI FspI FspAI HindIII HpaI MfeI MluI
NheI NotI NruI NsiI PacI PmeI PsiI PsrI
PvuI RsrII SalI SbfI SexAI SfiI SgfI SgrAI
SnaBI SpeI SrfI SspI SwaI TstI Tth111I XbaI
ZraI