(Circular) (Six-base) MAPSORT of: pFN21ASDB0014.seq Check: 6400 from: 1 to: 10609 pFN21ASDB0014 10609 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4528 4830 5848 6010 6610 7561 4528 Size: 302 1018 162 600 951 7576 Fragments arranged by size: 7576 1018 951 600 302 162 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4368 8419 278 Size: 53 83 186 936 2832 4051 2468 Fragments arranged by size: 4051 2832 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 7952 1937 Size: 6015 4594 Acc65I G'GTAC_C Cuts at: 7930 7930 Size: 10609 AclI AA'CG_TT Cuts at: 1563 8736 9109 1563 Size: 7173 373 3063 Fragments arranged by size: 7173 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2436 2906 3047 3728 4373 4502 5558 6899 Size: 804 470 141 681 645 129 1056 1341 Cuts at: 6899 7067 7298 7574 7658 8672 10056 10324 1632 Size: 168 231 276 84 1014 1384 268 1917 Fragments arranged by size: 1917 1384 1341 1056 1014 804 681 645 470 276 268 231 168 141 129 84 AfeI AGC'GCT Cuts at: 4811 6836 4811 Size: 2025 8584 AflII C'TTAA_G Cuts at: 820 1017 7391 820 Size: 197 6374 4038 Fragments arranged by size: 6374 4038 197 AflIII A'CryG_T Cuts at: 1203 2941 2973 9508 10485 1203 Size: 1738 32 6535 977 1327 Fragments arranged by size: 6535 1738 1327 977 32 AgeI A'CCGG_T Cuts at: 9440 9440 Size: 10609 AhdI GACnn_n'nnGTC Cuts at: 3109 5383 5905 9338 3109 Size: 2274 522 3433 4380 Fragments arranged by size: 4380 3433 2274 522 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 10609 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4626 7547 1184 Size: 3442 2921 4246 Fragments arranged by size: 4246 3442 2921 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2826 2858 3605 3637 2826 Size: 32 747 32 9798 Fragments arranged by size: 9798 747 32 32 AlwNI CAG_nnn'CTG Cuts at: 2593 2656 4822 4906 5158 5686 5791 5968 5995 Size: 63 2166 84 252 528 105 177 27 Cuts at: 5995 6286 6370 7369 8352 9924 2593 Size: 291 84 999 983 1572 3278 Fragments arranged by size: 3278 2166 1572 999 983 528 291 252 177 105 84 84 63 27 ApaI G_GGCC'C Cuts at: 4735 6475 6610 4735 Size: 1740 135 8734 Fragments arranged by size: 8734 1740 135 ApaLI G'TGCA_C Cuts at: 2536 8666 9822 2536 Size: 6130 1156 3323 Fragments arranged by size: 6130 3323 1156 ApoI r'AATT_y Cuts at: 1493 2732 6037 7918 8144 8296 10405 1493 Size: 1239 3305 1881 226 152 2109 1697 Fragments arranged by size: 3305 2109 1881 1697 1239 226 152 AseI AT'TA_AT Cuts at: 160 1032 9163 160 Size: 872 8131 1606 Fragments arranged by size: 8131 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10609 AvaI C'yCGr_G Cuts at: 1870 1943 2954 3586 3920 4115 5666 6435 6468 Size: 73 1011 632 334 195 1551 769 33 Cuts at: 6468 7457 7934 1870 Size: 989 477 4545 Fragments arranged by size: 4545 1551 1011 989 769 632 477 334 195 73 33 BamHI G'GATC_C Cuts at: 3369 3790 7939 8306 3369 Size: 421 4149 367 5672 Fragments arranged by size: 5672 4149 421 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2122 3353 3674 6156 7835 Size: 325 217 627 335 1231 321 2482 1679 Cuts at: 7835 7930 9385 618 Size: 95 1455 1842 Fragments arranged by size: 2482 1842 1679 1455 1231 627 335 325 321 217 95 BanII G_rGCy'C Cuts at: 1391 2289 2349 4269 4728 4735 5223 5244 5277 Size: 898 60 1920 459 7 488 21 33 Cuts at: 5277 5505 6475 6557 6610 7177 7928 1391 Size: 228 970 82 53 567 751 4072 Fragments arranged by size: 4072 1920 970 898 751 567 488 459 228 82 60 53 33 21 7 BbeI G_GCGC'C Cuts at: 3357 7839 3357 Size: 4482 6127 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2894 3073 4153 4297 5022 5171 5382 Size: 971 995 179 1080 144 725 149 211 Cuts at: 5382 5549 6266 6389 6641 928 Size: 167 717 123 252 4896 Fragments arranged by size: 4896 1080 995 971 725 717 252 211 179 167 149 144 123 BbvCI CC'TCA_GC Cuts at: 4004 4719 5201 6171 6423 7884 4004 Size: 715 482 970 252 1461 6729 Fragments arranged by size: 6729 1461 970 715 482 252 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2084 2118 4691 4725 8800 8834 1903 Size: 34 147 34 2573 34 4075 34 3678 Fragments arranged by size: 4075 3678 2573 147 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 8501 9711 8501 Size: 1210 9399 BclI T'GATC_A Cuts at: 1548 5106 7263 1548 Size: 3558 2157 4894 Fragments arranged by size: 4894 3558 2157 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2099 3266 3588 Size: 107 122 71 631 605 427 1167 322 Cuts at: 3588 4140 5288 6119 6719 7460 7622 7685 9220 Size: 552 1148 831 600 741 162 63 1535 Cuts at: 9220 136 Size: 1525 Fragments arranged by size: 1535 1525 1167 1148 831 741 631 605 600 552 427 322 162 122 107 71 63 BglII A'GATC_T Cuts at: 3393 4767 5358 10604 3393 Size: 1374 591 5246 3398 Fragments arranged by size: 5246 3398 1374 591 BlpI GC'TnA_GC Cuts at: 5996 8026 5996 Size: 2030 8579 Bme1580I G_kGCm'C Cuts at: 1790 2540 3677 4735 6475 6610 7421 7815 8670 Size: 750 1137 1058 1740 135 811 394 855 Cuts at: 8670 9826 1790 Size: 1156 2573 Fragments arranged by size: 2573 1740 1156 1137 1058 855 811 750 394 135 BmgBI CAC'GTC Cuts at: 1318 2944 1318 Size: 1626 8983 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2107 6718 8340 Size: 416 53 83 399 24 690 4611 1622 Cuts at: 8340 9298 442 Size: 958 1753 Fragments arranged by size: 4611 1753 1622 958 690 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10609 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3206 5206 3206 Size: 2000 8609 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2571 2663 2696 2895 3503 4283 4355 4442 Size: 1332 92 33 199 608 780 72 87 Cuts at: 4442 4673 4898 4934 4970 4991 5180 5201 5207 Size: 231 225 36 36 21 189 21 6 Cuts at: 5207 5261 5354 5402 5570 5657 5708 5960 6077 Size: 54 93 48 168 87 51 252 117 Cuts at: 6077 6107 6176 6224 6275 6359 6497 6527 6611 Size: 30 69 48 51 84 138 30 84 Cuts at: 6611 6674 6836 6887 6908 7223 7244 7256 7346 Size: 63 162 51 21 315 21 12 90 Cuts at: 7346 7499 7679 7742 9269 1239 Size: 153 180 63 1527 2579 Fragments arranged by size: 2579 1527 1332 780 608 315 252 231 225 199 189 180 168 162 153 138 117 93 92 90 87 87 84 84 72 69 63 63 54 51 51 51 48 48 36 36 33 30 30 21 21 21 21 12 6 Bpu10I CC'TnA_GC Cuts at: 4004 4719 5201 5504 6002 6171 6212 6423 7784 Size: 715 482 303 498 169 41 211 1361 Cuts at: 7784 7884 4004 Size: 100 6729 Fragments arranged by size: 6729 1361 715 498 482 303 211 169 100 41 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2144 2309 4886 5069 5228 5315 5999 7631 8734 Size: 165 2577 183 159 87 684 1632 1103 Cuts at: 8734 9599 9897 2144 Size: 865 298 2856 Fragments arranged by size: 2856 2577 1632 1103 865 684 298 183 165 159 87 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2978 3902 4341 6440 7493 9272 882 Size: 617 1479 924 439 2099 1053 1779 2219 Fragments arranged by size: 2219 2099 1779 1479 1053 924 617 439 BsaAI yAC'GTr Cuts at: 493 1204 2292 2602 7906 8400 493 Size: 711 1088 310 5304 494 2702 Fragments arranged by size: 5304 2702 1088 711 494 310 BsaBI GATnn'nnATC Cuts at: 2046 10603 2046 Size: 8557 2052 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2606 3110 3354 4365 Size: 53 83 186 936 1073 504 244 1011 Cuts at: 4365 7198 7728 7836 8416 8798 9433 275 Size: 2833 530 108 580 382 635 1451 Fragments arranged by size: 2833 1451 1073 1011 936 635 580 530 504 382 244 186 108 83 53 BsaWI w'CCGG_w Cuts at: 9042 9440 9714 9861 10272 9042 Size: 398 274 147 411 9379 Fragments arranged by size: 9379 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2702 2732 3605 3635 5492 Size: 30 165 30 1299 30 873 30 1857 Cuts at: 5492 5522 5805 5835 7427 7457 1178 Size: 30 283 30 1592 30 4330 Fragments arranged by size: 4330 1857 1592 1299 873 283 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2180 2707 3038 3506 3626 4352 4355 4568 Size: 994 527 331 468 120 726 3 213 Cuts at: 4568 4571 4589 4592 4736 4811 4814 4817 4820 Size: 3 18 3 144 75 3 3 3 Cuts at: 4820 4883 4895 4898 4901 4967 4970 5069 5072 Size: 63 12 3 3 66 3 99 3 Cuts at: 5072 5153 5282 5285 5567 5570 5633 5993 6074 Size: 81 129 3 282 3 63 360 81 Cuts at: 6074 6077 6080 6083 6188 6242 6392 6422 6641 Size: 3 3 3 105 54 150 30 219 Cuts at: 6641 6779 7022 7202 7241 7244 7253 7256 7496 Size: 138 243 180 39 3 9 3 240 Cuts at: 7496 7676 7688 1186 Size: 180 12 4107 Fragments arranged by size: 4107 994 726 527 468 360 331 282 243 240 219 213 180 180 150 144 138 129 120 105 99 81 81 75 66 63 63 54 39 30 18 12 12 9 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 5493 5946 7119 7218 7680 9812 10308 5493 Size: 453 1173 99 462 2132 496 5794 Fragments arranged by size: 5794 2132 1173 496 462 453 99 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2300 1163 Size: 573 564 9472 Fragments arranged by size: 9472 573 564 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6418 7273 7743 8059 8820 9848 Size: 275 186 4417 855 470 316 761 1028 Cuts at: 9848 1540 Size: 2301 Fragments arranged by size: 4417 2301 1028 855 761 470 316 275 186 BsiHKAI G_wGCw'C Cuts at: 2289 2540 2883 4248 4269 4728 5223 5244 5277 Size: 251 343 1365 21 459 495 21 33 Cuts at: 5277 5505 5757 6008 6557 8670 8755 9826 2289 Size: 228 252 251 549 2113 85 1071 3072 Fragments arranged by size: 3072 2113 1365 1071 549 495 459 343 252 251 251 228 85 33 21 21 BsiWI C'GTAC_G Cuts at: 2598 2598 Size: 10609 BsmI GAATG_Cn' Cuts at: 3591 7360 8120 8213 3591 Size: 3769 760 93 5987 Fragments arranged by size: 5987 3769 760 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4631 7730 4631 Size: 3099 7510 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2289 2349 2540 2883 3677 4248 4269 Size: 399 499 60 191 343 794 571 21 Cuts at: 4269 4728 4735 5223 5244 5277 5505 5757 6008 Size: 459 7 488 21 33 228 252 251 Cuts at: 6008 6475 6557 6610 7177 7421 7815 7928 8670 Size: 467 82 53 567 244 394 113 742 Cuts at: 8670 8755 9826 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 794 742 571 567 499 488 467 459 399 394 343 252 251 244 228 191 113 85 82 60 53 33 21 21 7 BspEI T'CCGG_A Cuts at: 10272 10272 Size: 10609 BspHI T'CATG_A Cuts at: 4248 8498 10228 4248 Size: 4250 1730 4629 Fragments arranged by size: 4629 4250 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2409 3552 4528 4830 5785 5848 6010 6303 Size: 1565 1143 976 302 955 63 162 293 Cuts at: 6303 6610 6714 6747 7299 7561 7806 7903 7964 Size: 307 104 33 552 262 245 97 61 Cuts at: 7964 844 Size: 3489 Fragments arranged by size: 3489 1565 1143 976 955 552 307 302 293 262 245 162 104 97 63 61 33 BsrBI CCG'CTC Cuts at: 1394 1823 2647 3094 3292 5698 6511 6733 7129 Size: 429 824 447 198 2406 813 222 396 Cuts at: 7129 7585 8496 1394 Size: 456 911 3507 Fragments arranged by size: 3507 2406 911 824 813 456 447 429 396 222 198 BsrDI GCAATG_nn' Cuts at: 65 1248 3378 5109 9104 9278 65 Size: 1183 2130 1731 3995 174 1396 Fragments arranged by size: 3995 2130 1731 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3383 3743 3896 4061 4316 4545 5985 Size: 110 1573 360 153 165 255 229 1440 Cuts at: 5985 7272 9253 9440 1700 Size: 1287 1981 187 2869 Fragments arranged by size: 2869 1981 1573 1440 1287 360 255 229 187 165 153 110 BsrGI T'GTAC_A Cuts at: 96 2433 96 Size: 2337 8272 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10609 BssSI C'ACGA_G Cuts at: 2453 2808 4049 4082 5570 6224 6584 7002 8669 Size: 355 1241 33 1488 654 360 418 1667 Cuts at: 8669 9681 2453 Size: 1012 3381 Fragments arranged by size: 3381 1667 1488 1241 1012 654 418 360 355 33 BstAPI GCAn_nnn'nTGC Cuts at: 794 2458 2656 4084 4906 5791 794 Size: 1664 198 1428 822 885 5612 Fragments arranged by size: 5612 1664 1428 885 822 198 BstBI TT'CG_AA Cuts at: 2007 9413 2007 Size: 7406 3203 BstEII G'GTnAC_C Cuts at: 1184 4921 5845 1184 Size: 3737 924 5948 Fragments arranged by size: 5948 3737 924 BstXI CCAn_nnnn'nTGG Cuts at: 2113 3994 4156 2113 Size: 1881 162 8566 Fragments arranged by size: 8566 1881 162 BstYI r'GATC_y Cuts at: 1972 3369 3393 3790 4188 4767 5337 5358 5580 Size: 1397 24 397 398 579 570 21 222 Cuts at: 5580 7011 7939 8306 8691 8708 10160 10246 10258 Size: 1431 928 367 385 17 1452 86 12 Cuts at: 10258 10604 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1431 1397 928 579 570 398 397 385 367 346 222 86 24 21 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2988 5613 6486 7095 513 Size: 552 957 966 2625 873 609 4027 Fragments arranged by size: 4027 2625 966 957 873 609 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3793 7198 487 Size: 683 56 2567 3405 3898 Fragments arranged by size: 3898 3405 2567 683 56 BtsI GCAGTG_nn' Cuts at: 3815 3914 4505 6852 8125 8919 8939 3815 Size: 99 591 2347 1273 794 20 5485 Fragments arranged by size: 5485 2347 1273 794 591 99 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10609 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 10574 DraI TTT'AAA Cuts at: 7913 7913 Size: 10609 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3842 4645 9616 809 Size: 507 2526 803 4971 1802 Fragments arranged by size: 4971 2526 1802 803 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3273 3894 3984 Size: 54 1425 50 275 18 1443 621 90 Cuts at: 3984 4291 4404 4566 4992 5256 5289 5709 5835 Size: 307 113 162 426 264 33 420 126 Cuts at: 5835 6120 6237 6849 6906 7740 8056 8945 10574 Size: 285 117 612 57 834 316 889 1629 Cuts at: 10574 8 Size: 43 Fragments arranged by size: 1629 1443 1425 889 834 621 612 426 420 316 307 285 275 264 162 126 117 113 90 57 54 50 43 33 18 EagI C'GGCC_G Cuts at: 1537 1812 7740 8056 1537 Size: 275 5928 316 4090 Fragments arranged by size: 5928 4090 316 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2172 2412 2441 3015 3464 4517 4758 5046 Size: 819 240 29 574 449 1053 241 288 Cuts at: 5046 5115 5295 5433 6176 6642 7043 7665 7851 Size: 69 180 138 743 466 401 622 186 Cuts at: 7851 8539 1353 Size: 688 3423 Fragments arranged by size: 3423 1053 819 743 688 622 574 466 449 401 288 241 240 186 180 138 69 29 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2807 4844 5642 6389 6443 6515 Size: 1064 138 1218 2037 798 747 54 72 Cuts at: 6515 9196 9570 9716 387 Size: 2681 374 146 1280 Fragments arranged by size: 2681 2037 1280 1218 1064 798 747 374 146 138 72 54 EcoICRI GAG'CTC Cuts at: 2287 4267 4726 5221 5242 5275 5503 6555 2287 Size: 1980 459 495 21 33 228 1052 6341 Fragments arranged by size: 6341 1980 1052 495 459 228 33 21 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2436 2571 2663 2696 2895 2906 3047 Size: 393 804 135 92 33 199 11 141 Cuts at: 3047 3503 3728 4283 4355 4373 4442 4502 4673 Size: 456 225 555 72 18 69 60 171 Cuts at: 4673 4898 4934 4970 4991 5180 5201 5207 5261 Size: 225 36 36 21 189 21 6 54 Cuts at: 5261 5354 5402 5558 5570 5657 5708 5960 6077 Size: 93 48 156 12 87 51 252 117 Cuts at: 6077 6107 6176 6224 6275 6359 6497 6527 6611 Size: 30 69 48 51 84 138 30 84 Cuts at: 6611 6674 6836 6887 6899 6908 7067 7223 7244 Size: 63 162 51 12 9 159 156 21 Cuts at: 7244 7256 7298 7346 7499 7574 7658 7679 7742 Size: 12 42 48 153 75 84 21 63 Cuts at: 7742 8672 9269 10056 10324 1239 Size: 930 597 787 268 1524 Fragments arranged by size: 1524 930 804 787 597 555 456 393 268 252 225 225 199 189 171 162 159 156 156 153 141 138 135 117 93 92 87 84 84 84 75 72 69 69 63 63 60 54 51 51 51 48 48 48 42 36 36 33 30 30 21 21 21 21 18 12 12 12 11 9 6 EcoNI CCTnn'n_nnAGG Cuts at: 2162 3485 3887 4337 4390 5552 5669 6374 7019 Size: 1323 402 450 53 1162 117 705 645 Cuts at: 7019 2162 Size: 5752 Fragments arranged by size: 5752 1323 1162 705 645 450 402 117 53 EcoO109I rG'GnC_Cy Cuts at: 3581 3924 4453 5428 6199 6208 6472 6544 6607 Size: 343 529 975 771 9 264 72 63 Cuts at: 6607 6736 6883 7795 3581 Size: 129 147 912 6395 Fragments arranged by size: 6395 975 912 771 529 343 264 147 129 72 63 9 EcoRI G'AATT_C Cuts at: 6037 7918 8296 6037 Size: 1881 378 8350 Fragments arranged by size: 8350 1881 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2158 6058 2158 Size: 3900 6709 FspI TGC'GCA Cuts at: 3317 5825 9115 3317 Size: 2508 3290 4811 Fragments arranged by size: 4811 3290 2508 FspAI rTGC'GCAy Cuts at: 5825 5825 Size: 10609 HaeII r_GCGC'y Cuts at: 1678 3357 4813 4896 5128 5453 6322 6838 7114 Size: 1679 1456 83 232 325 869 516 276 Cuts at: 7114 7513 7839 9756 1678 Size: 399 326 1917 2531 Fragments arranged by size: 2531 1917 1679 1456 869 516 399 326 325 276 232 83 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2449 2481 2702 2734 3095 Size: 32 135 32 877 32 221 32 361 Cuts at: 3095 3127 3385 3392 3417 3424 4173 4180 4205 Size: 32 258 7 25 7 749 7 25 Cuts at: 4205 4212 5048 5080 5336 5368 5369 5401 5492 Size: 7 836 32 256 32 1 32 91 Cuts at: 5492 5524 5891 5923 6439 6471 7610 7642 9250 Size: 32 367 32 516 32 1139 32 1608 Cuts at: 9250 9282 9324 9356 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1608 1139 877 836 749 516 367 361 258 256 221 135 91 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 25 25 7 7 7 7 1 HincII GTy'rAC Cuts at: 669 1657 1938 6703 7953 8199 669 Size: 988 281 4765 1250 246 3079 Fragments arranged by size: 4765 3079 1250 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 10609 HpaI GTT'AAC Cuts at: 8199 8199 Size: 10609 KasI G'GCGC_C Cuts at: 3353 7835 3353 Size: 4482 6127 KpnI G_GTAC'C Cuts at: 7934 7934 Size: 10609 MfeI C'AATT_G Cuts at: 8208 8208 Size: 10609 MluI A'CGCG_T Cuts at: 10485 10485 Size: 10609 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2154 2752 2883 3885 5445 5922 6675 7517 Size: 849 598 131 1002 1560 477 753 842 Cuts at: 7517 7875 9723 9907 1305 Size: 358 1848 184 2007 Fragments arranged by size: 2007 1848 1560 1002 849 842 753 598 477 358 184 131 MscI TGG'CCA Cuts at: 10 64 1489 1832 3275 3986 4293 4406 4568 Size: 54 1425 343 1443 711 307 113 162 Cuts at: 4568 4994 5258 5291 5711 10 Size: 426 264 33 420 4908 Fragments arranged by size: 4908 1443 1425 711 426 420 343 307 264 162 113 54 33 MslI CAynn'nnrTG Cuts at: 518 1166 2472 3206 4631 6536 8567 8926 9085 Size: 648 1306 734 1425 1905 2031 359 159 Cuts at: 9085 518 Size: 2042 Fragments arranged by size: 2042 2031 1905 1425 1306 734 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2083 2653 3131 3303 3421 3706 3982 4032 Size: 477 570 478 172 118 285 276 50 Cuts at: 4032 4096 4462 4531 4606 4675 4876 4912 5254 Size: 64 366 69 75 69 201 36 342 Cuts at: 5254 5489 5635 5782 5950 6055 6220 6643 6781 Size: 235 146 147 168 105 165 423 138 Cuts at: 6781 6940 7097 7164 7222 7369 7477 7489 7657 Size: 159 157 67 58 147 108 12 168 Cuts at: 7657 7684 8025 8702 9850 10095 1606 Size: 27 341 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 570 478 477 423 366 342 341 285 276 245 235 201 172 168 168 165 159 157 147 147 146 138 118 108 105 75 69 69 67 64 58 50 36 27 12 NaeI GCC'GGC Cuts at: 3385 3745 3898 4063 4547 3385 Size: 360 153 165 484 9447 Fragments arranged by size: 9447 484 360 165 153 NarI GG'CG_CC Cuts at: 3354 7836 3354 Size: 4482 6127 NcoI C'CATG_G Cuts at: 513 1065 2022 2988 6486 513 Size: 552 957 966 3498 4636 Fragments arranged by size: 4636 3498 966 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 10609 NgoMIV G'CCGG_C Cuts at: 3383 3743 3896 4061 4545 3383 Size: 360 153 165 484 9447 Fragments arranged by size: 9447 484 360 165 153 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10609 NotI GC'GGCC_GC Cuts at: 8056 8056 Size: 10609 NspI r_CATG'y Cuts at: 1132 1229 2977 3904 3949 6976 7967 9512 1132 Size: 97 1748 927 45 3027 991 1545 2229 Fragments arranged by size: 3027 2229 1748 1545 991 927 97 45 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 10609 PciI A'CATG_T Cuts at: 2973 9508 2973 Size: 6535 4074 PflMI CCAn_nnn'nTGG Cuts at: 1108 2593 3154 6370 6589 1108 Size: 1485 561 3216 219 5128 Fragments arranged by size: 5128 3216 1485 561 219 PfoI T'CCnGG_A Cuts at: 2673 2979 3372 3480 4164 4857 4968 5340 5370 Size: 306 393 108 684 693 111 372 30 Cuts at: 5370 5399 5583 6045 2673 Size: 29 184 462 7237 Fragments arranged by size: 7237 693 684 462 393 372 306 184 111 108 30 29 PmeI GTTT'AAAC Cuts at: 7913 7913 Size: 10609 PmlI CAC'GTG Cuts at: 2292 2292 Size: 10609 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3281 3313 3605 3637 8678 8710 10191 10223 3281 Size: 32 292 32 5041 32 1481 32 3667 Fragments arranged by size: 5041 3667 1481 292 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4453 5428 6199 6544 6736 6883 7795 4453 Size: 975 771 345 192 147 912 7267 Fragments arranged by size: 7267 975 912 771 345 192 147 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 10609 PsiI TTA'TAA Cuts at: 8179 8179 Size: 10609 PspOMI G'GGCC_C Cuts at: 4731 6471 6606 4731 Size: 1740 135 8734 Fragments arranged by size: 8734 1740 135 PspXI vC'TCGA_Gb Cuts at: 7457 7457 Size: 10609 PstI C_TGCA'G Cuts at: 830 2661 3453 3549 4394 4539 4647 4680 4827 Size: 1831 792 96 845 145 108 33 147 Cuts at: 4827 4911 5736 5787 5796 5859 5955 5976 6279 Size: 84 825 51 9 63 96 21 303 Cuts at: 6279 6669 6711 6744 6954 7296 7407 7572 7713 Size: 390 42 33 210 342 111 165 141 Cuts at: 7713 7961 830 Size: 248 3478 Fragments arranged by size: 3478 1831 845 825 792 390 342 303 248 210 165 147 145 141 111 108 96 96 84 63 51 42 33 33 21 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10609 PvuII CAG'CTG Cuts at: 2653 3131 3421 3706 3982 4096 4606 4675 4876 Size: 478 290 285 276 114 510 69 201 Cuts at: 4876 4912 5254 5489 5635 5782 5950 6055 6643 Size: 36 342 235 146 147 168 105 588 Cuts at: 6643 6781 6940 7222 7369 7477 7489 7657 2653 Size: 138 159 282 147 108 12 168 5605 Fragments arranged by size: 5605 588 510 478 342 290 285 282 276 235 201 168 168 159 147 147 146 138 114 108 105 69 36 12 RsrII CG'GwC_CG Cuts at: 7539 7539 Size: 10609 SacI G_AGCT'C Cuts at: 2289 4269 4728 5223 5244 5277 5505 6557 2289 Size: 1980 459 495 21 33 228 1052 6341 Fragments arranged by size: 6341 1980 1052 495 459 228 33 21 SacII CC_GC'GG Cuts at: 7098 7098 Size: 10609 SalI G'TCGA_C Cuts at: 1936 7951 1936 Size: 6015 4594 SapI GCTCTTCn'nnn_ Cuts at: 3464 4517 5115 5433 6642 7043 3464 Size: 1053 598 318 1209 401 7030 Fragments arranged by size: 7030 1209 1053 598 401 318 SbfI CC_TGCA'GG Cuts at: 4827 6711 6744 7961 4827 Size: 1884 33 1217 7475 Fragments arranged by size: 7475 1884 1217 33 ScaI AGT'ACT Cuts at: 8857 10569 8857 Size: 1712 8897 SexAI A'CCwGG_T Cuts at: 3888 3888 Size: 10609 SfcI C'TryA_G Cuts at: 826 1045 2494 2657 3449 3545 4390 4535 4643 Size: 219 1449 163 792 96 845 145 108 Cuts at: 4643 4676 4823 4907 5732 5783 5792 5855 5951 Size: 33 147 84 825 51 9 63 96 Cuts at: 5951 5972 6275 6665 6707 6740 6950 7292 7403 Size: 21 303 390 42 33 210 342 111 Cuts at: 7403 7568 7709 7957 9092 9773 9964 826 Size: 165 141 248 1135 681 191 1471 Fragments arranged by size: 1471 1449 1135 845 825 792 681 390 342 303 248 219 210 191 165 163 147 145 141 111 108 96 96 84 63 51 42 33 33 21 9 SfiI GGCCn_nnn'nGGCC Cuts at: 5288 6719 7685 5288 Size: 1431 966 8212 Fragments arranged by size: 8212 1431 966 SfoI GGC'GCC Cuts at: 3355 7837 3355 Size: 4482 6127 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10609 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10609 SmaI CCC'GGG Cuts at: 1872 2956 3588 3922 6470 7936 1872 Size: 1084 632 334 2548 1466 4545 Fragments arranged by size: 4545 2548 1466 1084 632 334 SmlI C'TyrA_G Cuts at: 820 1017 1943 2159 2324 4115 4865 5084 5243 Size: 197 926 216 165 1791 750 219 159 Cuts at: 5243 5330 5666 6014 7391 7457 7646 8713 9614 Size: 87 336 348 1377 66 189 1067 901 Cuts at: 9614 9876 820 Size: 262 1553 Fragments arranged by size: 1791 1553 1377 1067 926 901 750 348 336 262 219 216 197 189 165 159 87 66 SnaBI TAC'GTA Cuts at: 493 7906 8400 493 Size: 7413 494 2702 Fragments arranged by size: 7413 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 10609 SphI G_CATG'C Cuts at: 1132 6976 7967 1132 Size: 5844 991 3774 Fragments arranged by size: 5844 3774 991 SrfI GCCC'GGGC Cuts at: 6470 6470 Size: 10609 SspI AAT'ATT Cuts at: 5 52 8533 5 Size: 47 8481 2081 Fragments arranged by size: 8481 2081 47 StuI AGG'CCT Cuts at: 1517 2367 2681 3269 4385 7442 1517 Size: 850 314 588 1116 3057 4684 Fragments arranged by size: 4684 3057 1116 850 588 314 StyI C'CwwG_G Cuts at: 513 1065 2022 2265 2826 2988 3270 3276 4212 Size: 552 957 243 561 162 282 6 936 Cuts at: 4212 4518 4569 4995 5431 5922 6450 6486 6558 Size: 306 51 426 436 491 528 36 72 Cuts at: 6558 7503 8048 513 Size: 945 545 3074 Fragments arranged by size: 3074 957 945 936 561 552 545 528 491 436 426 306 282 243 162 72 51 36 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2110 2529 2906 3185 3692 7556 8636 Size: 1255 530 419 377 279 507 3864 1080 Cuts at: 8636 8653 8806 8991 9436 325 Size: 17 153 185 445 1498 Fragments arranged by size: 3864 1498 1255 1080 530 507 445 419 377 279 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2433 7434 7602 Size: 274 80 33 51 1443 456 5001 168 Cuts at: 7602 8855 10567 96 Size: 1253 1712 138 Fragments arranged by size: 5001 1712 1443 1253 456 274 168 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3599 3631 5805 5837 10569 10601 3599 Size: 32 2174 32 4732 32 3607 Fragments arranged by size: 4732 3607 2174 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5843 1316 Size: 4527 6082 XbaI T'CTAG_A Cuts at: 7945 7945 Size: 10609 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2191 2671 3004 3268 3478 4219 5857 6082 6565 Size: 480 333 264 210 741 1638 225 483 Cuts at: 6565 6691 2191 Size: 126 6109 Fragments arranged by size: 6109 1638 741 483 480 333 264 225 210 126 XhoI C'TCGA_G Cuts at: 1943 4115 5666 7457 1943 Size: 2172 1551 1791 5095 Fragments arranged by size: 5095 2172 1791 1551 XmaI C'CCGG_G Cuts at: 1870 2954 3586 3920 6468 7934 1870 Size: 1084 632 334 2548 1466 4545 Fragments arranged by size: 4545 2548 1466 1084 632 334 XmnI GAAnn'nnTTC Cuts at: 6041 8738 6041 Size: 2697 7912 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4366 8417 276 Size: 53 83 186 936 2832 4051 2468 Fragments arranged by size: 4051 2832 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BstZ17I Bsu36I DraIII EcoRV FseI NruI NsiI PacI PsrI SanDI SwaI