(Circular) (Six-base) MAPSORT of: pFN21ASDA1734.seq Check: 6026 from: 1 to: 8605 pFN21ASDA1734 8605 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2529 2751 4080 6415 Size: 53 83 186 936 993 222 1329 2335 Cuts at: 6415 278 Size: 2468 Fragments arranged by size: 2468 2335 1329 993 936 222 186 83 53 AccI GT'mk_AC Cuts at: 1937 2661 3831 5948 1937 Size: 724 1170 2117 4594 Fragments arranged by size: 4594 2117 1170 724 Acc65I G'GTAC_C Cuts at: 2400 5926 2400 Size: 3526 5079 AclI AA'CG_TT Cuts at: 1563 6732 7105 1563 Size: 5169 373 3063 Fragments arranged by size: 5169 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2708 4870 6668 8052 8320 1632 Size: 1076 2162 1798 1384 268 1917 Fragments arranged by size: 2162 1917 1798 1384 1076 268 AfeI AGC'GCT Cuts at: 3077 3077 Size: 8605 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8408 AflIII A'CryG_T Cuts at: 1203 7504 8481 1203 Size: 6301 977 1327 Fragments arranged by size: 6301 1327 977 AgeI A'CCGG_T Cuts at: 7436 7436 Size: 8605 AhdI GACnn_n'nnGTC Cuts at: 2218 7334 2218 Size: 5116 3489 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8605 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2612 1184 Size: 1428 7177 AlwNI CAG_nnn'CTG Cuts at: 2197 2857 3824 4519 5316 5494 5644 6348 7920 Size: 660 967 695 797 178 150 704 1572 Cuts at: 7920 2197 Size: 2882 Fragments arranged by size: 2882 1572 967 797 704 695 660 178 150 ApaI G_GGCC'C Cuts at: 4997 4997 Size: 8605 ApaLI G'TGCA_C Cuts at: 2243 3749 5884 6662 7818 2243 Size: 1506 2135 778 1156 3030 Fragments arranged by size: 3030 2135 1506 1156 778 ApoI r'AATT_y Cuts at: 1493 5914 6140 6292 8401 1493 Size: 4421 226 152 2109 1697 Fragments arranged by size: 4421 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 7159 160 Size: 872 6127 1606 Fragments arranged by size: 6127 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8605 AvaI C'yCGr_G Cuts at: 1870 1943 2317 2469 2770 3243 3627 4566 4697 Size: 73 374 152 301 473 384 939 131 Cuts at: 4697 5381 5930 1870 Size: 684 549 4545 Fragments arranged by size: 4545 939 684 549 473 384 374 301 152 131 73 AvrII C'CTAG_G Cuts at: 3019 3019 Size: 8605 BamHI G'GATC_C Cuts at: 2029 5935 6302 2029 Size: 3906 367 4332 Fragments arranged by size: 4332 3906 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2125 2131 2400 2464 2558 Size: 325 217 627 338 6 269 64 94 Cuts at: 2558 2702 2732 5156 5506 5633 5704 5926 7381 Size: 144 30 2424 350 127 71 222 1455 Cuts at: 7381 618 Size: 1842 Fragments arranged by size: 2424 1842 1455 627 350 338 325 269 222 217 144 127 94 71 64 30 6 BanII G_rGCy'C Cuts at: 1391 2318 3164 3381 4030 4296 4529 4997 5535 Size: 927 846 217 649 266 233 468 538 Cuts at: 5535 5618 5924 1391 Size: 83 306 4072 Fragments arranged by size: 4072 927 846 649 538 468 306 266 233 217 83 BbeI G_GCGC'C Cuts at: 2129 2135 2706 2736 2129 Size: 6 571 30 7998 Fragments arranged by size: 7998 571 30 6 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3082 3267 4625 4798 928 Size: 971 1183 185 1358 173 4735 Fragments arranged by size: 4735 1358 1183 971 185 173 BbvCI CC'TCA_GC Cuts at: 3682 3893 5650 3682 Size: 211 1757 6637 Fragments arranged by size: 6637 1757 211 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2503 2537 3320 3354 6796 6830 1903 Size: 34 566 34 783 34 3442 34 3678 Fragments arranged by size: 3678 3442 783 566 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6497 7707 6497 Size: 1210 7395 BclI T'GATC_A Cuts at: 1548 4347 1548 Size: 2799 5806 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2090 4406 4702 Size: 107 122 71 631 605 418 2316 296 Cuts at: 4702 5154 7216 136 Size: 452 2062 1525 Fragments arranged by size: 2316 2062 1525 631 605 452 418 296 122 107 71 BglII A'GATC_T Cuts at: 2835 8600 2835 Size: 5765 2840 BlpI GC'TnA_GC Cuts at: 2879 3057 6022 2879 Size: 178 2965 5462 Fragments arranged by size: 5462 2965 178 Bme1580I G_kGCm'C Cuts at: 1790 2247 2469 2975 3753 4671 4997 5070 5159 Size: 457 222 506 778 918 326 73 89 Cuts at: 5159 5511 5638 5888 6666 7822 1790 Size: 352 127 250 778 1156 2573 Fragments arranged by size: 2573 1156 918 778 778 506 457 352 326 250 222 127 89 73 BmgBI CAC'GTC Cuts at: 1318 2761 3691 3913 1318 Size: 1443 930 222 6010 Fragments arranged by size: 6010 1443 930 222 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3229 5706 6336 Size: 416 53 83 399 24 1812 2477 630 Cuts at: 6336 7294 442 Size: 958 1753 Fragments arranged by size: 2477 1812 1753 958 630 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 5658 1056 Size: 4602 4003 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4825 4825 Size: 8605 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2046 2064 2827 3801 4331 4505 4838 4851 Size: 807 18 763 974 530 174 333 13 Cuts at: 4851 5192 5498 7265 1239 Size: 341 306 1767 2579 Fragments arranged by size: 2579 1767 974 807 763 530 341 333 306 174 18 13 Bpu10I CC'TnA_GC Cuts at: 3682 3893 5650 3682 Size: 211 1757 6637 Fragments arranged by size: 6637 1757 211 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4478 4598 6730 7595 7893 4478 Size: 120 2132 865 298 5190 Fragments arranged by size: 5190 2132 865 298 120 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2426 2780 5678 7268 882 Size: 617 927 354 2898 1590 2219 Fragments arranged by size: 2898 2219 1590 927 617 354 BsaAI yAC'GTr Cuts at: 493 1204 2386 5902 6396 493 Size: 711 1182 3516 494 2702 Fragments arranged by size: 3516 2702 1182 711 494 BsaBI GATnn'nnATC Cuts at: 8599 8599 Size: 8605 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2126 2132 2412 2526 Size: 53 83 186 936 593 6 280 114 Cuts at: 2526 2703 2733 2748 3326 4077 4776 6412 6794 Size: 177 30 15 578 751 699 1636 382 Cuts at: 6794 7429 275 Size: 635 1451 Fragments arranged by size: 1636 1451 936 751 699 635 593 578 382 280 186 177 114 83 53 30 15 6 BsaWI w'CCGG_w Cuts at: 5745 7038 7436 7710 7857 8268 5745 Size: 1293 398 274 147 411 6082 Fragments arranged by size: 6082 1293 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2135 2165 2222 2252 3713 Size: 30 165 30 732 30 57 30 1461 Cuts at: 3713 3743 3774 3804 4085 4115 4562 4592 1178 Size: 30 31 30 281 30 447 30 5191 Fragments arranged by size: 5191 1461 732 447 281 165 57 31 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2312 2315 2366 3335 3389 3499 4020 4181 Size: 1126 3 51 969 54 110 521 161 Cuts at: 4181 4304 4661 4664 1186 Size: 123 357 3 5127 Fragments arranged by size: 5127 1126 969 521 357 161 123 110 54 51 3 3 BseYI C'CCAG_C Cuts at: 2104 2650 3094 4134 4242 4730 5059 5361 5536 Size: 546 444 1040 108 488 329 302 175 Cuts at: 5536 7808 8304 2104 Size: 2272 496 2405 Fragments arranged by size: 2405 2272 1040 546 496 488 444 329 302 175 108 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2939 3390 3425 5369 1163 Size: 573 1203 451 35 1944 4399 Fragments arranged by size: 4399 1944 1203 573 451 35 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3865 6055 6816 7844 1540 Size: 275 186 1864 2190 761 1028 2301 Fragments arranged by size: 2301 2190 1864 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2247 2318 2327 2714 2820 2880 3753 4278 5217 Size: 71 9 387 106 60 873 525 939 Cuts at: 5217 5888 6666 6751 7822 2247 Size: 671 778 85 1071 3030 Fragments arranged by size: 3030 1071 939 873 778 671 525 387 106 85 71 60 9 BsmI GAATG_Cn' Cuts at: 3722 6116 6209 3722 Size: 2394 93 6118 Fragments arranged by size: 6118 2394 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3041 3317 4241 3041 Size: 276 924 7405 Fragments arranged by size: 7405 924 276 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2247 2318 2327 2469 2714 2820 2880 Size: 399 457 71 9 142 245 106 60 Cuts at: 2880 2975 3164 3381 3753 4030 4278 4296 4529 Size: 95 189 217 372 277 248 18 233 Cuts at: 4529 4671 4997 5070 5159 5217 5511 5535 5618 Size: 142 326 73 89 58 294 24 83 Cuts at: 5618 5638 5888 5924 6666 6751 7822 1391 Size: 20 250 36 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 457 399 372 326 294 277 250 248 245 233 217 189 142 142 106 95 89 85 83 73 71 60 58 36 24 20 18 9 BspEI T'CCGG_A Cuts at: 8268 8268 Size: 8605 BspHI T'CATG_A Cuts at: 6494 8224 6494 Size: 1730 6875 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2604 2911 3190 3878 5960 844 Size: 1760 307 279 688 2082 3489 Fragments arranged by size: 3489 2082 1760 688 307 279 BsrBI CCG'CTC Cuts at: 1394 1823 2060 3540 3729 6492 1394 Size: 429 237 1480 189 2763 3507 Fragments arranged by size: 3507 2763 1480 429 237 189 BsrDI GCAATG_nn' Cuts at: 65 1248 2631 3954 7100 7274 65 Size: 1183 1383 1323 3146 174 1396 Fragments arranged by size: 3146 1396 1383 1323 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2128 2183 4318 5267 7249 7436 1700 Size: 110 318 55 2135 949 1982 187 2869 Fragments arranged by size: 2869 2135 1982 949 318 187 110 55 BsrGI T'GTAC_A Cuts at: 96 4143 96 Size: 4047 4558 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8605 BssSI C'ACGA_G Cuts at: 2348 3383 3550 6665 7677 2348 Size: 1035 167 3115 1012 3276 Fragments arranged by size: 3276 3115 1035 1012 167 BstAPI GCAn_nnn'nTGC Cuts at: 794 2197 2565 3205 794 Size: 1403 368 640 6194 Fragments arranged by size: 6194 1403 640 368 BstBI TT'CG_AA Cuts at: 2007 7409 2007 Size: 5402 3203 BstEII G'GTnAC_C Cuts at: 1184 3502 3667 1184 Size: 2318 165 6122 Fragments arranged by size: 6122 2318 165 BstXI CCAn_nnnn'nTGG Cuts at: 3349 4760 5099 3349 Size: 1411 339 6855 Fragments arranged by size: 6855 1411 339 BstYI r'GATC_y Cuts at: 1972 2029 2835 4479 5935 6302 6687 6704 8156 Size: 57 806 1644 1456 367 385 17 1452 Cuts at: 8156 8242 8254 8600 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1644 1456 1452 806 385 367 346 86 57 17 12 Bsu36I CC'TnA_GG Cuts at: 3318 4329 4932 5577 3318 Size: 1011 603 645 6346 Fragments arranged by size: 6346 1011 645 603 BtgI C'CryG_G Cuts at: 513 1065 2022 2233 2269 2308 2377 3255 3711 Size: 552 957 211 36 39 69 878 456 Cuts at: 3711 4392 4404 513 Size: 681 12 4714 Fragments arranged by size: 4714 957 878 681 552 456 211 69 39 36 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7866 Fragments arranged by size: 7866 683 56 BtsI GCAGTG_nn' Cuts at: 3663 4671 4763 4925 5104 5492 6121 6915 6935 Size: 1008 92 162 179 388 629 794 20 Cuts at: 6935 3663 Size: 5333 Fragments arranged by size: 5333 1008 794 629 388 179 162 92 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8605 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8570 DraI TTT'AAA Cuts at: 5909 5909 Size: 8605 DraIII CAC_nnn'GTG Cuts at: 3237 5714 3237 Size: 2477 6128 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2543 3827 5194 7612 809 Size: 507 1227 1284 1367 2418 1802 Fragments arranged by size: 2418 1802 1367 1284 1227 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3168 3862 4389 Size: 54 1425 50 275 18 1338 694 527 Cuts at: 4389 4407 4635 5101 5563 6052 6941 8570 8 Size: 18 228 466 462 489 889 1629 43 Fragments arranged by size: 1629 1425 1338 889 694 527 489 466 462 275 228 54 50 43 18 18 EagI C'GGCC_G Cuts at: 1537 1812 3862 6052 1537 Size: 275 2050 2190 4090 Fragments arranged by size: 4090 2190 2050 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2783 2907 3746 3924 4350 4979 5759 5801 Size: 1430 124 839 178 426 629 780 42 Cuts at: 5801 6535 1353 Size: 734 3423 Fragments arranged by size: 3423 1430 839 780 734 629 426 178 124 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2042 7192 7566 7712 387 Size: 1064 138 453 5150 374 146 1280 Fragments arranged by size: 5150 1280 1064 453 374 146 138 EcoICRI GAG'CTC Cuts at: 2316 2316 Size: 8605 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2046 2064 2708 2827 3801 4331 4505 Size: 393 414 18 644 119 974 530 174 Cuts at: 4505 4838 4851 4870 5192 5498 6668 7265 8052 Size: 333 13 19 322 306 1170 597 787 Cuts at: 8052 8320 1239 Size: 268 1524 Fragments arranged by size: 1524 1170 974 787 644 597 530 414 393 333 322 306 268 174 119 19 18 13 EcoNI CCTnn'n_nnAGG Cuts at: 5207 5864 5207 Size: 657 7948 EcoO109I rG'GnC_Cy Cuts at: 2071 2593 4993 4994 2071 Size: 522 2400 1 5682 Fragments arranged by size: 5682 2400 522 1 EcoRI G'AATT_C Cuts at: 5914 6292 5914 Size: 378 8227 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4900 4900 Size: 8605 FspI TGC'GCA Cuts at: 7111 7111 Size: 8605 HaeII r_GCGC'y Cuts at: 1678 2129 2135 2197 2706 2736 3079 3187 4794 Size: 451 6 62 509 30 343 108 1607 Cuts at: 4794 4913 5336 7752 1678 Size: 119 423 2416 2531 Fragments arranged by size: 2531 2416 1607 509 451 423 343 119 108 62 30 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2135 2167 2204 2236 2270 Size: 32 135 32 563 32 37 32 34 Cuts at: 2270 2302 2463 2495 2947 2979 4034 4066 4332 Size: 32 161 32 452 32 1055 32 266 Cuts at: 4332 4364 4691 4723 4961 4993 7246 7278 7320 Size: 32 327 32 238 32 2253 32 42 Cuts at: 7320 7352 1373 Size: 32 2626 Fragments arranged by size: 2626 2253 1055 563 452 327 266 238 161 135 42 37 34 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2530 2584 5949 6195 669 Size: 988 281 592 54 3365 246 3079 Fragments arranged by size: 3365 3079 988 592 281 246 54 HindIII A'AGCT_T Cuts at: 748 748 Size: 8605 HpaI GTT'AAC Cuts at: 6195 6195 Size: 8605 KasI G'GCGC_C Cuts at: 2125 2131 2702 2732 2125 Size: 6 571 30 7998 Fragments arranged by size: 7998 571 30 6 KpnI G_GTAC'C Cuts at: 2404 5930 2404 Size: 3526 5079 MfeI C'AATT_G Cuts at: 6204 6204 Size: 8605 MluI A'CGCG_T Cuts at: 8481 8481 Size: 8605 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2721 3756 4565 7719 7903 1305 Size: 1416 1035 809 3154 184 2007 Fragments arranged by size: 3154 2007 1416 1035 809 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3170 4391 4637 10 Size: 54 1425 343 1338 1221 246 3978 Fragments arranged by size: 3978 1425 1338 1221 343 246 54 MslI CAynn'nnrTG Cuts at: 518 1166 4505 6563 6922 7081 518 Size: 648 3339 2058 359 159 2042 Fragments arranged by size: 3339 2058 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2310 2379 3061 3400 3546 4203 4242 4370 Size: 704 69 682 339 146 657 39 128 Cuts at: 4370 4516 5365 5540 6021 6698 7846 8091 1606 Size: 146 849 175 481 677 1148 245 2120 Fragments arranged by size: 2120 1148 849 704 682 677 657 481 339 245 175 146 146 128 69 39 NaeI GCC'GGC Cuts at: 2130 2185 5269 2130 Size: 55 3084 5466 Fragments arranged by size: 5466 3084 55 NarI GG'CG_CC Cuts at: 2126 2132 2703 2733 2126 Size: 6 571 30 7998 Fragments arranged by size: 7998 571 30 6 NcoI C'CATG_G Cuts at: 513 1065 2022 3255 4392 4404 513 Size: 552 957 1233 1137 12 4714 Fragments arranged by size: 4714 1233 1137 957 552 12 NdeI CA'TA_TG Cuts at: 387 387 Size: 8605 NgoMIV G'CCGG_C Cuts at: 2128 2183 5267 2128 Size: 55 3084 5466 Fragments arranged by size: 5466 3084 55 NheI G'CTAG_C Cuts at: 1052 5654 1052 Size: 4602 4003 NotI GC'GGCC_GC Cuts at: 6052 6052 Size: 8605 NspI r_CATG'y Cuts at: 1132 1229 2426 2491 5878 5963 7508 1132 Size: 97 1197 65 3387 85 1545 2229 Fragments arranged by size: 3387 2229 1545 1197 97 85 65 PasI CC'CwG_GG Cuts at: 1348 3151 5611 1348 Size: 1803 2460 4342 Fragments arranged by size: 4342 2460 1803 PciI A'CATG_T Cuts at: 7504 7504 Size: 8605 PflMI CCAn_nnn'nTGG Cuts at: 1108 3256 5644 1108 Size: 2148 2388 4069 Fragments arranged by size: 4069 2388 2148 PfoI T'CCnGG_A Cuts at: 4455 4482 4455 Size: 27 8578 PmeI GTTT'AAAC Cuts at: 5909 5909 Size: 8605 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6674 6706 8187 8219 6674 Size: 32 1481 32 7060 Fragments arranged by size: 7060 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2593 2593 Size: 8605 PshAI GACnn'nnGTC Cuts at: 1613 2772 1613 Size: 1159 7446 PsiI TTA'TAA Cuts at: 6175 6175 Size: 8605 PspOMI G'GGCC_C Cuts at: 4993 4993 Size: 8605 PstI C_TGCA'G Cuts at: 830 2601 5295 5957 830 Size: 1771 2694 662 3478 Fragments arranged by size: 3478 2694 1771 662 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8605 PvuII CAG'CTG Cuts at: 3400 4242 4370 4516 5365 5540 3400 Size: 842 128 146 849 175 6465 Fragments arranged by size: 6465 849 842 175 146 128 SacI G_AGCT'C Cuts at: 2318 2318 Size: 8605 SacII CC_GC'GG Cuts at: 2311 2380 2311 Size: 69 8536 SalI G'TCGA_C Cuts at: 1936 5947 1936 Size: 4011 4594 SbfI CC_TGCA'GG Cuts at: 5295 5957 5295 Size: 662 7943 ScaI AGT'ACT Cuts at: 6853 8565 6853 Size: 1712 6893 SexAI A'CCwGG_T Cuts at: 2214 2214 Size: 8605 SfcI C'TryA_G Cuts at: 826 1045 2597 4601 5291 5541 5953 7088 7769 Size: 219 1552 2004 690 250 412 1135 681 Cuts at: 7769 7960 826 Size: 191 1471 Fragments arranged by size: 2004 1552 1471 1135 690 681 412 250 219 191 SfoI GGC'GCC Cuts at: 2127 2133 2704 2734 2127 Size: 6 571 30 7998 Fragments arranged by size: 7998 571 30 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8605 SgrAI Cr'CCGG_yG Cuts at: 1700 2128 1700 Size: 428 8177 SmaI CCC'GGG Cuts at: 1872 3245 5932 1872 Size: 1373 2687 4545 Fragments arranged by size: 4545 2687 1373 SmlI C'TyrA_G Cuts at: 820 1017 1943 3627 4493 4613 6709 7610 7872 Size: 197 926 1684 866 120 2096 901 262 Cuts at: 7872 820 Size: 1553 Fragments arranged by size: 2096 1684 1553 926 901 866 262 197 120 SnaBI TAC'GTA Cuts at: 493 5902 6396 493 Size: 5409 494 2702 Fragments arranged by size: 5409 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8605 SphI G_CATG'C Cuts at: 1132 5878 5963 1132 Size: 4746 85 3774 Fragments arranged by size: 4746 3774 85 SspI AAT'ATT Cuts at: 5 52 6529 5 Size: 47 6477 2081 Fragments arranged by size: 6477 2081 47 StuI AGG'CCT Cuts at: 1517 5289 5606 1517 Size: 3772 317 4516 Fragments arranged by size: 4516 3772 317 StyI C'CwwG_G Cuts at: 513 1065 2022 3019 3099 3255 4392 4404 4794 Size: 552 957 997 80 156 1137 12 390 Cuts at: 4794 5227 5511 5808 6044 513 Size: 433 284 297 236 3074 Fragments arranged by size: 3074 1137 997 957 552 433 390 297 284 236 156 80 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3023 3248 4459 5298 5725 6632 6649 Size: 1255 1443 225 1211 839 427 907 17 Cuts at: 6649 6802 6987 7432 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1443 1255 1211 907 839 445 427 225 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4143 6851 8563 Size: 274 80 33 51 1443 2166 2708 1712 Cuts at: 8563 96 Size: 138 Fragments arranged by size: 2708 2166 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2477 2509 3774 3806 4085 4117 5125 5157 8565 Size: 32 1265 32 279 32 1008 32 3408 Cuts at: 8565 8597 2477 Size: 32 2485 Fragments arranged by size: 3408 2485 1265 1008 279 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2981 3995 1316 Size: 1665 1014 5926 Fragments arranged by size: 5926 1665 1014 XbaI T'CTAG_A Cuts at: 5941 5941 Size: 8605 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2819 4306 4480 5362 2819 Size: 1487 174 882 6062 Fragments arranged by size: 6062 1487 882 174 XhoI C'TCGA_G Cuts at: 1943 3627 1943 Size: 1684 6921 XmaI C'CCGG_G Cuts at: 1870 3243 5930 1870 Size: 1373 2687 4545 Fragments arranged by size: 4545 2687 1373 XmnI GAAnn'nnTTC Cuts at: 3884 4883 6734 3884 Size: 999 1851 5755 Fragments arranged by size: 5755 1851 999 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2527 2749 4078 6413 Size: 53 83 186 936 993 222 1329 2335 Cuts at: 6413 276 Size: 2468 Fragments arranged by size: 2468 2335 1329 993 936 222 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BaeI BfrBI BsiWI BstZ17I EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SapI SfiI SrfI SwaI