Restriction map for
pFN21ASDA1734
(Linear) (Six-base) MAPSORT of: KIAA1734.seq Check: 1466 from: 1 to: 3876
KIAA1734.orf
With 182 enzymes: SgfI *
February 28, 2007 11:29 ..
AatII G_ACGT'C
Cuts at: 0 506 728 2057 3876
Size: 506 222 1329 1819
Fragments arranged by size:
1819 1329 506 222
AccI GT'mk_AC
Cuts at: 0 638 1808 3876
Size: 638 1170 2068
Fragments arranged by size:
2068 1170 638
Acc65I G'GTAC_C
Cuts at: 0 377 3876
Size: 377 3499
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 685 2847 3876
Size: 685 2162 1029
Fragments arranged by size:
2162 1029 685
AfeI AGC'GCT
Cuts at: 0 1054 3876
Size: 1054 2822
AhdI GACnn_n'nnGTC
Cuts at: 0 195 3876
Size: 195 3681
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 589 3876
Size: 589 3287
AlwNI CAG_nnn'CTG
Cuts at: 0 174 834 1801 2496 3293 3471 3621 3876
Size: 174 660 967 695 797 178 150 255
Fragments arranged by size:
967 797 695 660 255 178 174 150
ApaI G_GGCC'C
Cuts at: 0 2974 3876
Size: 2974 902
ApaLI G'TGCA_C
Cuts at: 0 220 1726 3861 3876
Size: 220 1506 2135 15
Fragments arranged by size:
2135 1506 220 15
AvaI C'yCGr_G
Cuts at: 0 294 446 747 1220 1604 2543 2674 3358
Size: 294 152 301 473 384 939 131 684
Cuts at: 3358 3876
Size: 518
Fragments arranged by size:
939 684 518 473 384 301 294 152
131
AvrII C'CTAG_G
Cuts at: 0 996 3876
Size: 996 2880
BamHI G'GATC_C
Cuts at: 0 6 3876
Size: 6 3870
BanI G'GyrC_C
Cuts at: 0 102 108 377 441 535 679 709 3133
Size: 102 6 269 64 94 144 30 2424
Cuts at: 3133 3483 3610 3681 3876
Size: 350 127 71 195
Fragments arranged by size:
2424 350 269 195 144 127 102 94
71 64 30 6
BanII G_rGCy'C
Cuts at: 0 295 1141 1358 2007 2273 2506 2974 3512
Size: 295 846 217 649 266 233 468 538
Cuts at: 3512 3595 3876
Size: 83 281
Fragments arranged by size:
846 649 538 468 295 281 266 233
217 83
BbeI G_GCGC'C
Cuts at: 0 106 112 683 713 3876
Size: 106 6 571 30 3163
Fragments arranged by size:
3163 571 106 30 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 1059 1244 2602 2775 3876
Size: 1059 185 1358 173 1101
Fragments arranged by size:
1358 1101 1059 185 173
BbvCI CC'TCA_GC
Cuts at: 0 1659 1870 3627 3876
Size: 1659 211 1757 249
Fragments arranged by size:
1757 1659 249 211
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 480 514 1297 1331 3876
Size: 480 34 783 34 2545
Fragments arranged by size:
2545 783 480 34 34
BclI T'GATC_A
Cuts at: 0 2324 3876
Size: 2324 1552
BglI GCCn_nnn'nGGC
Cuts at: 0 67 2383 2679 3131 3876
Size: 67 2316 296 452 745
Fragments arranged by size:
2316 745 452 296 67
BglII A'GATC_T
Cuts at: 0 812 3876
Size: 812 3064
BlpI GC'TnA_GC
Cuts at: 0 856 1034 3876
Size: 856 178 2842
Fragments arranged by size:
2842 856 178
Bme1580I G_kGCm'C
Cuts at: 0 224 446 952 1730 2648 2974 3047 3136
Size: 224 222 506 778 918 326 73 89
Cuts at: 3136 3488 3615 3865 3876
Size: 352 127 250 11
Fragments arranged by size:
918 778 506 352 326 250 224 222
127 89 73 11
BmgBI CAC'GTC
Cuts at: 0 738 1668 1890 3876
Size: 738 930 222 1986
Fragments arranged by size:
1986 930 738 222
BmrI ACTGGGnnnn_n'
Cuts at: 0 1206 3683 3876
Size: 1206 2477 193
Fragments arranged by size:
2477 1206 193
BmtI G_CTAG'C
Cuts at: 0 3635 3876
Size: 3635 241
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2802 3876
Size: 2802 1074
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 23 41 804 1778 2308 2482 2815 2828
Size: 23 18 763 974 530 174 333 13
Cuts at: 2828 3169 3475 3876
Size: 341 306 401
Fragments arranged by size:
974 763 530 401 341 333 306 174
23 18 13
Bpu10I CC'TnA_GC
Cuts at: 0 1659 1870 3627 3876
Size: 1659 211 1757 249
Fragments arranged by size:
1757 1659 249 211
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2455 2575 3876
Size: 2455 120 1301
Fragments arranged by size:
2455 1301 120
BsaI GGTCTCn'nnnn_
Cuts at: 0 403 757 3655 3876
Size: 403 354 2898 221
Fragments arranged by size:
2898 403 354 221
BsaAI yAC'GTr
Cuts at: 0 363 3876
Size: 363 3513
BsaHI Gr'CG_yC
Cuts at: 0 103 109 389 503 680 710 725 1303
Size: 103 6 280 114 177 30 15 578
Cuts at: 1303 2054 2753 3876
Size: 751 699 1123
Fragments arranged by size:
1123 751 699 578 280 177 114 103
30 15 6
BsaWI w'CCGG_w
Cuts at: 0 3722 3876
Size: 3722 154
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 112 142 199 229 1690 1720 1751 1781
Size: 112 30 57 30 1461 30 31 30
Cuts at: 1781 2062 2092 2539 2569 3876
Size: 281 30 447 30 1307
Fragments arranged by size:
1461 1307 447 281 112 57 31 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 289 292 343 1312 1366 1476 1997 2158
Size: 289 3 51 969 54 110 521 161
Cuts at: 2158 2281 2638 2641 3876
Size: 123 357 3 1235
Fragments arranged by size:
1235 969 521 357 289 161 123 110
54 51 3 3
BseYI C'CCAG_C
Cuts at: 0 81 627 1071 2111 2219 2707 3036 3338
Size: 81 546 444 1040 108 488 329 302
Cuts at: 3338 3513 3876
Size: 175 363
Fragments arranged by size:
1040 546 488 444 363 329 302 175
108 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 916 1367 1402 3346 3876
Size: 916 451 35 1944 530
Fragments arranged by size:
1944 916 530 451 35
BsiEI CG_ry'CG
Cuts at: 0 1842 3876
Size: 1842 2034
BsiHKAI G_wGCw'C
Cuts at: 0 224 295 304 691 797 857 1730 2255
Size: 224 71 9 387 106 60 873 525
Cuts at: 2255 3194 3865 3876
Size: 939 671 11
Fragments arranged by size:
939 873 671 525 387 224 106 71
60 11 9
BsmI GAATG_Cn'
Cuts at: 0 1699 3876
Size: 1699 2177
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1018 1294 2218 3876
Size: 1018 276 924 1658
Fragments arranged by size:
1658 1018 924 276
Bsp1286I G_dGCh'C
Cuts at: 0 224 295 304 446 691 797 857 952
Size: 224 71 9 142 245 106 60 95
Cuts at: 952 1141 1358 1730 2007 2255 2273 2506 2648
Size: 189 217 372 277 248 18 233 142
Cuts at: 2648 2974 3047 3136 3194 3488 3512 3595 3615
Size: 326 73 89 58 294 24 83 20
Cuts at: 3615 3865 3876
Size: 250 11
Fragments arranged by size:
372 326 294 277 250 248 245 233
224 217 189 142 142 106 95 89
83 73 71 60 58 24 20 18
11 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 581 888 1167 1855 3876
Size: 581 307 279 688 2021
Fragments arranged by size:
2021 688 581 307 279
BsrBI CCG'CTC
Cuts at: 0 37 1517 1706 3876
Size: 37 1480 189 2170
Fragments arranged by size:
2170 1480 189 37
BsrDI GCAATG_nn'
Cuts at: 0 608 1931 3876
Size: 608 1323 1945
Fragments arranged by size:
1945 1323 608
BsrFI r'CCGG_y
Cuts at: 0 105 160 2295 3244 3876
Size: 105 55 2135 949 632
Fragments arranged by size:
2135 949 632 105 55
BsrGI T'GTAC_A
Cuts at: 0 2120 3876
Size: 2120 1756
BssSI C'ACGA_G
Cuts at: 0 325 1360 1527 3876
Size: 325 1035 167 2349
Fragments arranged by size:
2349 1035 325 167
BstAPI GCAn_nnn'nTGC
Cuts at: 0 174 542 1182 3876
Size: 174 368 640 2694
Fragments arranged by size:
2694 640 368 174
BstEII G'GTnAC_C
Cuts at: 0 1479 1644 3876
Size: 1479 165 2232
Fragments arranged by size:
2232 1479 165
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1326 2737 3076 3876
Size: 1326 1411 339 800
Fragments arranged by size:
1411 1326 800 339
BstYI r'GATC_y
Cuts at: 0 6 812 2456 3876
Size: 6 806 1644 1420
Fragments arranged by size:
1644 1420 806 6
Bsu36I CC'TnA_GG
Cuts at: 0 1295 2306 2909 3554 3876
Size: 1295 1011 603 645 322
Fragments arranged by size:
1295 1011 645 603 322
BtgI C'CryG_G
Cuts at: 0 210 246 285 354 1232 1688 2369 2381
Size: 210 36 39 69 878 456 681 12
Cuts at: 2381 3876
Size: 1495
Fragments arranged by size:
1495 878 681 456 210 69 39 36
12
BtsI GCAGTG_nn'
Cuts at: 0 1640 2648 2740 2902 3081 3469 3876
Size: 1640 1008 92 162 179 388 407
Fragments arranged by size:
1640 1008 407 388 179 162 92
DraIII CAC_nnn'GTG
Cuts at: 0 1214 3691 3876
Size: 1214 2477 185
Fragments arranged by size:
2477 1214 185
DrdI GACnn_nn'nnGTC
Cuts at: 0 520 1804 3171 3876
Size: 520 1284 1367 705
Fragments arranged by size:
1367 1284 705 520
EaeI y'GGCC_r
Cuts at: 0 1145 1839 2366 2384 2612 3078 3540 3876
Size: 1145 694 527 18 228 466 462 336
Fragments arranged by size:
1145 694 527 466 462 336 228 18
EagI C'GGCC_G
Cuts at: 0 1839 3876
Size: 1839 2037
EarI CTCTTCn'nnn_
Cuts at: 0 760 884 1723 1901 2327 2956 3736 3778
Size: 760 124 839 178 426 629 780 42
Cuts at: 3778 3876
Size: 98
Fragments arranged by size:
839 780 760 629 426 178 124 98
42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 3876
Size: 19 3857
EcoICRI GAG'CTC
Cuts at: 0 293 3876
Size: 293 3583
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 23 41 685 804 1778 2308 2482 2815
Size: 23 18 644 119 974 530 174 333
Cuts at: 2815 2828 2847 3169 3475 3876
Size: 13 19 322 306 401
Fragments arranged by size:
974 644 530 401 333 322 306 174
119 23 19 18 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3184 3841 3876
Size: 3184 657 35
Fragments arranged by size:
3184 657 35
EcoO109I rG'GnC_Cy
Cuts at: 0 48 570 2970 2971 3876
Size: 48 522 2400 1 905
Fragments arranged by size:
2400 905 522 48 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2877 3876
Size: 2877 999
HaeII r_GCGC'y
Cuts at: 0 106 112 174 683 713 1056 1164 2771
Size: 106 6 62 509 30 343 108 1607
Cuts at: 2771 2890 3313 3876
Size: 119 423 563
Fragments arranged by size:
1607 563 509 423 343 119 108 106
62 30 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 112 144 181 213 247 279 440 472
Size: 112 32 37 32 34 32 161 32
Cuts at: 472 924 956 2011 2043 2309 2341 2668 2700
Size: 452 32 1055 32 266 32 327 32
Cuts at: 2700 2938 2970 3876
Size: 238 32 906
Fragments arranged by size:
1055 906 452 327 266 238 161 112
37 34 32 32 32 32 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 507 561 3876
Size: 507 54 3315
Fragments arranged by size:
3315 507 54
KasI G'GCGC_C
Cuts at: 0 102 108 679 709 3876
Size: 102 6 571 30 3167
Fragments arranged by size:
3167 571 102 30 6
KpnI G_GTAC'C
Cuts at: 0 381 3876
Size: 381 3495
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 698 1733 2542 3876
Size: 698 1035 809 1334
Fragments arranged by size:
1334 1035 809 698
MscI TGG'CCA
Cuts at: 0 1147 2368 2614 3876
Size: 1147 1221 246 1262
Fragments arranged by size:
1262 1221 1147 246
MslI CAynn'nnrTG
Cuts at: 0 2482 3876
Size: 2482 1394
MspA1I CmG'CkG
Cuts at: 0 287 356 1038 1377 1523 2180 2219 2347
Size: 287 69 682 339 146 657 39 128
Cuts at: 2347 2493 3342 3517 3876
Size: 146 849 175 359
Fragments arranged by size:
849 682 657 359 339 287 175 146
146 128 69 39
NaeI GCC'GGC
Cuts at: 0 107 162 3246 3876
Size: 107 55 3084 630
Fragments arranged by size:
3084 630 107 55
NarI GG'CG_CC
Cuts at: 0 103 109 680 710 3876
Size: 103 6 571 30 3166
Fragments arranged by size:
3166 571 103 30 6
NcoI C'CATG_G
Cuts at: 0 1232 2369 2381 3876
Size: 1232 1137 12 1495
Fragments arranged by size:
1495 1232 1137 12
NgoMIV G'CCGG_C
Cuts at: 0 105 160 3244 3876
Size: 105 55 3084 632
Fragments arranged by size:
3084 632 105 55
NheI G'CTAG_C
Cuts at: 0 3631 3876
Size: 3631 245
NspI r_CATG'y
Cuts at: 0 403 468 3855 3876
Size: 403 65 3387 21
Fragments arranged by size:
3387 403 65 21
PasI CC'CwG_GG
Cuts at: 0 1128 3588 3876
Size: 1128 2460 288
Fragments arranged by size:
2460 1128 288
PflMI CCAn_nnn'nTGG
Cuts at: 0 1233 3621 3876
Size: 1233 2388 255
Fragments arranged by size:
2388 1233 255
PfoI T'CCnGG_A
Cuts at: 0 2432 2459 3876
Size: 2432 27 1417
Fragments arranged by size:
2432 1417 27
PpuMI rG'GwC_Cy
Cuts at: 0 570 3876
Size: 570 3306
PshAI GACnn'nnGTC
Cuts at: 0 749 3876
Size: 749 3127
PspOMI G'GGCC_C
Cuts at: 0 2970 3876
Size: 2970 906
PstI C_TGCA'G
Cuts at: 0 578 3272 3876
Size: 578 2694 604
Fragments arranged by size:
2694 604 578
PvuII CAG'CTG
Cuts at: 0 1377 2219 2347 2493 3342 3517 3876
Size: 1377 842 128 146 849 175 359
Fragments arranged by size:
1377 849 842 359 175 146 128
SacI G_AGCT'C
Cuts at: 0 295 3876
Size: 295 3581
SacII CC_GC'GG
Cuts at: 0 288 357 3876
Size: 288 69 3519
Fragments arranged by size:
3519 288 69
SbfI CC_TGCA'GG
Cuts at: 0 3272 3876
Size: 3272 604
SexAI A'CCwGG_T
Cuts at: 0 191 3876
Size: 191 3685
SfcI C'TryA_G
Cuts at: 0 574 2578 3268 3518 3876
Size: 574 2004 690 250 358
Fragments arranged by size:
2004 690 574 358 250
SfoI GGC'GCC
Cuts at: 0 104 110 681 711 3876
Size: 104 6 571 30 3165
Fragments arranged by size:
3165 571 104 30 6
SgrAI Cr'CCGG_yG
Cuts at: 0 105 3876
Size: 105 3771
SmaI CCC'GGG
Cuts at: 0 1222 3876
Size: 1222 2654
SmlI C'TyrA_G
Cuts at: 0 1604 2470 2590 3876
Size: 1604 866 120 1286
Fragments arranged by size:
1604 1286 866 120
SphI G_CATG'C
Cuts at: 0 3855 3876
Size: 3855 21
StuI AGG'CCT
Cuts at: 0 3266 3583 3876
Size: 3266 317 293
Fragments arranged by size:
3266 317 293
StyI C'CwwG_G
Cuts at: 0 996 1076 1232 2369 2381 2771 3204 3488
Size: 996 80 156 1137 12 390 433 284
Cuts at: 3488 3785 3876
Size: 297 91
Fragments arranged by size:
1137 996 433 390 297 284 156 91
80 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1000 1225 2436 3275 3702 3876
Size: 1000 225 1211 839 427 174
Fragments arranged by size:
1211 1000 839 427 225 174
TatI w'GTAC_w
Cuts at: 0 2120 3876
Size: 2120 1756
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 454 486 1751 1783 2062 2094 3102 3134
Size: 454 32 1265 32 279 32 1008 32
Cuts at: 3134 3876
Size: 742
Fragments arranged by size:
1265 1008 742 454 279 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 958 1972 3876
Size: 958 1014 1904
Fragments arranged by size:
1904 1014 958
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 796 2283 2457 3339 3876
Size: 796 1487 174 882 537
Fragments arranged by size:
1487 882 796 537 174
XhoI C'TCGA_G
Cuts at: 0 1604 3876
Size: 1604 2272
XmaI C'CCGG_G
Cuts at: 0 1220 3876
Size: 1220 2656
XmnI GAAnn'nnTTC
Cuts at: 0 1861 2860 3876
Size: 1861 999 1016
Fragments arranged by size:
1861 1016 999
ZraI GAC'GTC
Cuts at: 0 504 726 2055 3876
Size: 504 222 1329 1821
Fragments arranged by size:
1821 1329 504 222
Enzymes that do cut:
AatII AccI Acc65I AcuI AfeI AhdI AlfI AlwNI
ApaI ApaLI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII KasI
KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NspI PasI PflMI PfoI PpuMI PshAI
PspOMI PstI PvuII SacI SacII SbfI SexAI SfcI
SfoI SgrAI SmaI SmlI SphI StuI StyI TaqII
TatI TstI Tth111I XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AclI AflII AflIII AgeI AleI AloI ApoI
AscI AseI AsiSI BaeI BciVI BfrBI BsaBI BsiWI
BspEI BspHI BssHII BstBI BstZ17I BtgZI ClaI CspCI
DraI EcoRI EcoRV FseI FspI FspAI HindIII HpaI
MfeI MluI NdeI NotI NruI NsiI PacI PciI
PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII
SalI SanDI SapI ScaI SfiI SgfI SnaBI SpeI
SrfI SspI SwaI XbaI