Restriction map for
pFN21ASDA1027
(Linear) (Six-base) MAPSORT of: KIAA1027.seq Check: 2543 from: 1 to: 7623
KIAA1027.orf
With 182 enzymes: SgfI *
February 28, 2007 11:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3364 6504 7623
Size: 3364 3140 1119
Fragments arranged by size:
3364 3140 1119
AatII G_ACGT'C
Cuts at: 0 3427 7623
Size: 3427 4196
AccI GT'mk_AC
Cuts at: 0 64 1249 5013 6887 7623
Size: 64 1185 3764 1874 736
Fragments arranged by size:
3764 1874 1185 736 64
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 283 805 1169 1219 1451 2077 3029
Size: 37 246 522 364 50 232 626 952
Cuts at: 3029 3816 5186 6079 6814 6881 6997 7351 7586
Size: 787 1370 893 735 67 116 354 235
Cuts at: 7586 7623
Size: 37
Fragments arranged by size:
1370 952 893 787 735 626 522 364
354 246 235 232 116 67 50 37
37
AfeI AGC'GCT
Cuts at: 0 2943 5949 7623
Size: 2943 3006 1674
Fragments arranged by size:
3006 2943 1674
AflII C'TTAA_G
Cuts at: 0 432 2568 6385 7623
Size: 432 2136 3817 1238
Fragments arranged by size:
3817 2136 1238 432
AflIII A'CryG_T
Cuts at: 0 4157 7623
Size: 4157 3466
AhdI GACnn_n'nnGTC
Cuts at: 0 1269 7623
Size: 1269 6354
AleI CACnn'nnGTG
Cuts at: 0 4489 7623
Size: 4489 3134
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3569 7310 7623
Size: 3569 3741 313
Fragments arranged by size:
3741 3569 313
AlwNI CAG_nnn'CTG
Cuts at: 0 1908 2374 2667 2748 2850 3270 3315 3433
Size: 1908 466 293 81 102 420 45 118
Cuts at: 3433 3585 3798 4027 5311 5766 6468 7623
Size: 152 213 229 1284 455 702 1155
Fragments arranged by size:
1908 1284 1155 702 466 455 420 293
229 213 152 118 102 81 45
ApaI G_GGCC'C
Cuts at: 0 746 4857 5650 6435 7623
Size: 746 4111 793 785 1188
Fragments arranged by size:
4111 1188 793 785 746
ApaLI G'TGCA_C
Cuts at: 0 4056 7070 7623
Size: 4056 3014 553
Fragments arranged by size:
4056 3014 553
ApoI r'AATT_y
Cuts at: 0 1383 7623
Size: 1383 6240
AvaI C'yCGr_G
Cuts at: 0 617 2085 3212 3362 3779 3803 4850 4871
Size: 617 1468 1127 150 417 24 1047 21
Cuts at: 4871 4881 5159 6428 6983 7623
Size: 10 278 1269 555 640
Fragments arranged by size:
1468 1269 1127 1047 640 617 555 417
278 150 24 21 10
BamHI G'GATC_C
Cuts at: 0 104 7623
Size: 104 7519
BanI G'GyrC_C
Cuts at: 0 2169 3082 4068 5803 5920 7129 7623
Size: 2169 913 986 1735 117 1209 494
Fragments arranged by size:
2169 1735 1209 986 913 494 117
BanII G_rGCy'C
Cuts at: 0 146 746 1898 3298 3897 4391 4793 4857
Size: 146 600 1152 1400 599 494 402 64
Cuts at: 4857 5650 5849 6435 6708 6848 6926 7623
Size: 793 199 586 273 140 78 697
Fragments arranged by size:
1400 1152 793 697 600 599 586 494
402 273 199 146 140 78 64
BbeI G_GCGC'C
Cuts at: 0 5807 7623
Size: 5807 1816
BbsI GAAGACnn'nnnn_
Cuts at: 0 49 298 1033 3923 6067 6524 7623
Size: 49 249 735 2890 2144 457 1099
Fragments arranged by size:
2890 2144 1099 735 457 249 49
BbvCI CC'TCA_GC
Cuts at: 0 2531 2681 2778 6212 7623
Size: 2531 150 97 3434 1411
Fragments arranged by size:
3434 2531 1411 150 97
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3760 3794 4300 4334 7623
Size: 3760 34 506 34 3289
Fragments arranged by size:
3760 3289 506 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1477 1519 1585 2263 2371 2521 2767 3118
Size: 1477 42 66 678 108 150 246 351
Cuts at: 3118 3562 3611 3640 4291 5341 5695 6439 6949
Size: 444 49 29 651 1050 354 744 510
Cuts at: 6949 7623
Size: 674
Fragments arranged by size:
1477 1050 744 678 674 651 510 444
354 351 246 150 108 66 49 42
29
BglII A'GATC_T
Cuts at: 0 833 3186 5123 7623
Size: 833 2353 1937 2500
Fragments arranged by size:
2500 2353 1937 833
BlpI GC'TnA_GC
Cuts at: 0 1892 2804 2945 3297 7623
Size: 1892 912 141 352 4326
Fragments arranged by size:
4326 1892 912 352 141
Bme1580I G_kGCm'C
Cuts at: 0 351 746 980 1878 2130 3085 4060 4668
Size: 351 395 234 898 252 955 975 608
Cuts at: 4668 4857 5650 5862 6435 7074 7623
Size: 189 793 212 573 639 549
Fragments arranged by size:
975 955 898 793 639 608 573 549
395 351 252 234 212 189
BmgBI CAC'GTC
Cuts at: 0 5889 7623
Size: 5889 1734
BmrI ACTGGGnnnn_n'
Cuts at: 0 3325 3704 4132 4662 5816 6138 7623
Size: 3325 379 428 530 1154 322 1485
Fragments arranged by size:
3325 1485 1154 530 428 379 322
BmtI G_CTAG'C
Cuts at: 0 904 7623
Size: 904 6719
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1350 1647 5094 5898 7623
Size: 1350 297 3447 804 1725
Fragments arranged by size:
3447 1725 1350 804 297
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 1279 1385 1485 1813 2569 2770 4150
Size: 205 1074 106 100 328 756 201 1380
Cuts at: 4150 4717 4728 4949 4996 5407 5887 6457 7623
Size: 567 11 221 47 411 480 570 1166
Fragments arranged by size:
1380 1166 1074 756 570 567 480 411
328 221 205 201 106 100 47 11
Bpu10I CC'TnA_GC
Cuts at: 0 2531 2681 2778 3122 5257 6212 7623
Size: 2531 150 97 344 2135 955 1411
Fragments arranged by size:
2531 2135 1411 955 344 150 97
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 898 2035 3976 5066 7623
Size: 898 1137 1941 1090 2557
Fragments arranged by size:
2557 1941 1137 1090 898
BsaI GGTCTCn'nnnn_
Cuts at: 0 249 5886 6217 6269 7623
Size: 249 5637 331 52 1354
Fragments arranged by size:
5637 1354 331 249 52
BsaAI yAC'GTr
Cuts at: 0 894 7623
Size: 894 6729
BsaHI Gr'CG_yC
Cuts at: 0 80 2906 3424 5804 7623
Size: 80 2826 518 2380 1819
Fragments arranged by size:
2826 2380 1819 518 80
BsaWI w'CCGG_w
Cuts at: 0 3101 7623
Size: 3101 4522
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 673 703 913 943 7623
Size: 673 30 210 30 6680
Fragments arranged by size:
6680 673 210 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 571 1258 1448 1731 2665 3514 4669 5404
Size: 571 687 190 283 934 849 1155 735
Cuts at: 5404 5653 7039 7623
Size: 249 1386 584
Fragments arranged by size:
1386 1155 934 849 735 687 584 571
283 249 190
BseYI C'CCAG_C
Cuts at: 0 117 130 190 1073 1412 1472 1921 2069
Size: 117 13 60 883 339 60 449 148
Cuts at: 2069 2374 2455 2843 2924 2938 3308 3739 4061
Size: 305 81 388 81 14 370 431 322
Cuts at: 4061 4206 5174 5178 5461 5643 5766 5908 6112
Size: 145 968 4 283 182 123 142 204
Cuts at: 6112 6189 7307 7623
Size: 77 1118 316
Fragments arranged by size:
1118 968 883 449 431 388 370 339
322 316 305 283 204 182 148 145
142 123 117 81 81 77 60 60
14 13 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 670 1537 1745 2761 2878 2920 3140 3401
Size: 670 867 208 1016 117 42 220 261
Cuts at: 3401 3460 3790 4304 4493 5419 5835 6215 6694
Size: 59 330 514 189 926 416 380 479
Cuts at: 6694 7623
Size: 929
Fragments arranged by size:
1016 929 926 867 670 514 479 416
380 330 261 220 208 189 117 59
42
BsiHKAI G_wGCw'C
Cuts at: 0 563 3298 4060 4475 5849 6848 6926 7074
Size: 563 2735 762 415 1374 999 78 148
Cuts at: 7074 7622 7623
Size: 548 1
Fragments arranged by size:
2735 1374 999 762 563 548 415 148
78 1
BsmI GAATG_Cn'
Cuts at: 0 2724 4748 7623
Size: 2724 2024 2875
Fragments arranged by size:
2875 2724 2024
BsmBI CGTCTCn'nnnn_
Cuts at: 0 559 1088 4105 7623
Size: 559 529 3017 3518
Fragments arranged by size:
3518 3017 559 529
Bsp1286I G_dGCh'C
Cuts at: 0 146 351 563 746 980 1878 1898 2130
Size: 146 205 212 183 234 898 20 232
Cuts at: 2130 3085 3298 3897 4060 4391 4475 4668 4793
Size: 955 213 599 163 331 84 193 125
Cuts at: 4793 4857 5650 5849 5862 6435 6708 6848 6926
Size: 64 793 199 13 573 273 140 78
Cuts at: 6926 7074 7622 7623
Size: 148 548 1
Fragments arranged by size:
955 898 793 599 573 548 331 273
234 232 213 212 205 199 193 183
163 148 146 140 125 84 78 64
20 13 1
BspEI T'CCGG_A
Cuts at: 0 3101 7623
Size: 3101 4522
BspHI T'CATG_A
Cuts at: 0 335 369 7623
Size: 335 34 7254
Fragments arranged by size:
7254 335 34
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1916 3340 3364 4262 6504 7278 7623
Size: 1916 1424 24 898 2242 774 345
Fragments arranged by size:
2242 1916 1424 898 774 345 24
BsrBI CCG'CTC
Cuts at: 0 102 5220 5785 7623
Size: 102 5118 565 1838
Fragments arranged by size:
5118 1838 565 102
BsrDI GCAATG_nn'
Cuts at: 0 448 1172 2957 3342 4991 5588 5963 6379
Size: 448 724 1785 385 1649 597 375 416
Cuts at: 6379 6602 6908 7052 7361 7490 7623
Size: 223 306 144 309 129 133
Fragments arranged by size:
1785 1649 724 597 448 416 385 375
309 306 223 144 133 129
BsrFI r'CCGG_y
Cuts at: 0 1177 2836 3719 4900 7623
Size: 1177 1659 883 1181 2723
Fragments arranged by size:
2723 1659 1181 1177 883
BsrGI T'GTAC_A
Cuts at: 0 627 4575 7242 7623
Size: 627 3948 2667 381
Fragments arranged by size:
3948 2667 627 381
BssSI C'ACGA_G
Cuts at: 0 562 656 7623
Size: 562 94 6967
Fragments arranged by size:
6967 562 94
BstAPI GCAn_nnn'nTGC
Cuts at: 0 492 1515 2032 2289 2667 3355 4327 5239
Size: 492 1023 517 257 378 688 972 912
Cuts at: 5239 5311 5455 7234 7623
Size: 72 144 1779 389
Fragments arranged by size:
1779 1023 972 912 688 517 492 389
378 257 144 72
BstEII G'GTnAC_C
Cuts at: 0 5350 5613 5790 6363 7623
Size: 5350 263 177 573 1260
Fragments arranged by size:
5350 1260 573 263 177
BstXI CCAn_nnnn'nTGG
Cuts at: 0 987 1942 2022 2064 2238 3078 3765 4491
Size: 987 955 80 42 174 840 687 726
Cuts at: 4491 4593 6096 6270 6360 7074 7149 7623
Size: 102 1503 174 90 714 75 474
Fragments arranged by size:
1503 987 955 840 726 714 687 474
174 174 102 90 80 75 42
BstYI r'GATC_y
Cuts at: 0 104 266 731 833 1625 2582 3186 3429
Size: 104 162 465 102 792 957 604 243
Cuts at: 3429 5123 7571 7623
Size: 1694 2448 52
Fragments arranged by size:
2448 1694 957 792 604 465 243 162
104 102 52
Bsu36I CC'TnA_GG
Cuts at: 0 3637 3791 3810 4769 7623
Size: 3637 154 19 959 2854
Fragments arranged by size:
3637 2854 959 154 19
BtgI C'CryG_G
Cuts at: 0 68 525 1764 2237 2444 3968 4784 5772
Size: 68 457 1239 473 207 1524 816 988
Cuts at: 5772 6152 6401 6548 7623
Size: 380 249 147 1075
Fragments arranged by size:
1524 1239 1075 988 816 473 457 380
249 207 147 68
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2778 3288 3635 7488 7623
Size: 2778 510 347 3853 135
Fragments arranged by size:
3853 2778 510 347 135
BtsI GCAGTG_nn'
Cuts at: 0 1721 1815 1859 1878 2879 2890 3443 3578
Size: 1721 94 44 19 1001 11 553 135
Cuts at: 3578 5108 5240 5330 5600 5818 5996 6254 7168
Size: 1530 132 90 270 218 178 258 914
Cuts at: 7168 7391 7623
Size: 223 232
Fragments arranged by size:
1721 1530 1001 914 553 270 258 232
223 218 178 135 132 94 90 44
19 11
ClaI AT'CG_AT
Cuts at: 0 1187 7623
Size: 1187 6436
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 837 872 4380 4415 6806 6841 6891 6926
Size: 837 35 3508 35 2391 35 50 35
Cuts at: 6926 7623
Size: 697
Fragments arranged by size:
3508 2391 837 697 50 35 35 35
35
DraIII CAC_nnn'GTG
Cuts at: 0 2125 3163 4055 4300 5190 7623
Size: 2125 1038 892 245 890 2433
Fragments arranged by size:
2433 2125 1038 892 890 245
DrdI GACnn_nn'nnGTC
Cuts at: 0 1758 6091 6904 7623
Size: 1758 4333 813 719
Fragments arranged by size:
4333 1758 813 719
EaeI y'GGCC_r
Cuts at: 0 1431 1566 2385 2696 3461 3519 3612 4074
Size: 1431 135 819 311 765 58 93 462
Cuts at: 4074 4595 4898 4902 4988 5147 5679 6717 7205
Size: 521 303 4 86 159 532 1038 488
Cuts at: 7205 7623
Size: 418
Fragments arranged by size:
1431 1038 819 765 532 521 488 462
418 311 303 159 135 93 86 58
4
EarI CTCTTCn'nnn_
Cuts at: 0 398 5723 7436 7541 7623
Size: 398 5325 1713 105 82
Fragments arranged by size:
5325 1713 398 105 82
EcoICRI GAG'CTC
Cuts at: 0 3296 5847 6846 6924 7623
Size: 3296 2551 999 78 699
Fragments arranged by size:
3296 2551 999 699 78
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 205 283 805 1169 1219 1279 1385
Size: 37 168 78 522 364 50 60 106
Cuts at: 1385 1451 1485 1813 2077 2569 2770 3029 3816
Size: 66 34 328 264 492 201 259 787
Cuts at: 3816 4150 4717 4728 4949 4996 5186 5407 5887
Size: 334 567 11 221 47 190 221 480
Cuts at: 5887 6079 6457 6814 6881 6997 7351 7586 7623
Size: 192 378 357 67 116 354 235 37
Fragments arranged by size:
787 567 522 492 480 378 364 357
354 334 328 264 259 235 221 221
201 192 190 168 116 106 78 67
66 60 50 47 37 37 34 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 799 943 1825 3496 5026 5389 5443 7623
Size: 799 144 882 1671 1530 363 54 2180
Fragments arranged by size:
2180 1671 1530 882 799 363 144 54
EcoO109I rG'GnC_Cy
Cuts at: 0 114 428 621 743 1689 1839 2328 3267
Size: 114 314 193 122 946 150 489 939
Cuts at: 3267 3531 3795 3960 4643 4854 4875 5019 5424
Size: 264 264 165 683 211 21 144 405
Cuts at: 5424 5647 6093 6216 6432 6897 7173 7623
Size: 223 446 123 216 465 276 450
Fragments arranged by size:
946 939 683 489 465 450 446 405
314 276 264 264 223 216 211 193
165 150 144 123 122 114 21
EcoRV GAT'ATC
Cuts at: 0 1191 7623
Size: 1191 6432
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 789 1239 7540 7623
Size: 789 450 6301 83
Fragments arranged by size:
6301 789 450 83
FseI GG_CCGG'CC
Cuts at: 0 4904 7623
Size: 4904 2719
FspI TGC'GCA
Cuts at: 0 1870 2692 2714 7623
Size: 1870 822 22 4909
Fragments arranged by size:
4909 1870 822 22
HaeII r_GCGC'y
Cuts at: 0 2748 2945 4569 4636 5807 5951 7623
Size: 2748 197 1624 67 1171 144 1672
Fragments arranged by size:
2748 1672 1624 1171 197 144 67
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 650 682 1231 1255 1263 1287 1564 1596
Size: 650 32 549 24 8 24 277 32
Cuts at: 1596 1617 1649 3414 3446 3567 3599 5115 5147
Size: 21 32 1765 32 121 32 1516 32
Cuts at: 5147 6266 6298 7330 7362 7623
Size: 1119 32 1032 32 261
Fragments arranged by size:
1765 1516 1119 1032 650 549 277 261
121 32 32 32 32 32 32 32
32 24 24 21 8
HincII GTy'rAC
Cuts at: 0 1286 6806 7623
Size: 1286 5520 817
Fragments arranged by size:
5520 1286 817
HindIII A'AGCT_T
Cuts at: 0 197 1092 3223 7623
Size: 197 895 2131 4400
Fragments arranged by size:
4400 2131 895 197
KasI G'GCGC_C
Cuts at: 0 5803 7623
Size: 5803 1820
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 1769 3627 3932 4754 6294 7623
Size: 511 1258 1858 305 822 1540 1329
Fragments arranged by size:
1858 1540 1329 1258 822 511 305
MscI TGG'CCA
Cuts at: 0 1568 2698 3521 3614 4597 5149 7623
Size: 1568 1130 823 93 983 552 2474
Fragments arranged by size:
2474 1568 1130 983 823 552 93
MslI CAynn'nnrTG
Cuts at: 0 328 334 500 3925 4051 4489 5101 5593
Size: 328 6 166 3425 126 438 612 492
Cuts at: 5593 5777 5986 6529 7623
Size: 184 209 543 1094
Fragments arranged by size:
3425 1094 612 543 492 438 328 209
184 166 126 6
MspA1I CmG'CkG
Cuts at: 0 121 633 1430 1654 1921 2038 2073 2317
Size: 121 512 797 224 267 117 35 244
Cuts at: 2317 2632 2664 2680 2710 3007 3057 3517 3549
Size: 315 32 16 30 297 50 460 32
Cuts at: 3549 3593 3602 4002 4012 4318 4523 4974 5145
Size: 44 9 400 10 306 205 451 171
Cuts at: 5145 5178 5664 5710 6112 6304 6975 7240 7623
Size: 33 486 46 402 192 671 265 383
Fragments arranged by size:
797 671 512 486 460 451 402 400
383 315 306 297 267 265 244 224
205 192 171 121 117 50 46 44
35 33 32 32 30 16 10 9
NaeI GCC'GGC
Cuts at: 0 1179 2838 4902 7623
Size: 1179 1659 2064 2721
Fragments arranged by size:
2721 2064 1659 1179
NarI GG'CG_CC
Cuts at: 0 5804 7623
Size: 5804 1819
NcoI C'CATG_G
Cuts at: 0 68 525 2444 4784 5772 6548 7623
Size: 68 457 1919 2340 988 776 1075
Fragments arranged by size:
2340 1919 1075 988 776 457 68
NgoMIV G'CCGG_C
Cuts at: 0 1177 2836 4900 7623
Size: 1177 1659 2064 2723
Fragments arranged by size:
2723 2064 1659 1177
NheI G'CTAG_C
Cuts at: 0 900 7623
Size: 900 6723
NspI r_CATG'y
Cuts at: 0 216 333 1404 1455 1509 2353 2758 3134
Size: 216 117 1071 51 54 844 405 376
Cuts at: 3134 4161 7623
Size: 1027 3462
Fragments arranged by size:
3462 1071 1027 844 405 376 216 117
54 51
PasI CC'CwG_GG
Cuts at: 0 3330 6270 7170 7623
Size: 3330 2940 900 453
Fragments arranged by size:
3330 2940 900 453
PciI A'CATG_T
Cuts at: 0 4157 7623
Size: 4157 3466
PflMI CCAn_nnn'nTGG
Cuts at: 0 609 2148 3270 3315 4264 4440 5892 7623
Size: 609 1539 1122 45 949 176 1452 1731
Fragments arranged by size:
1731 1539 1452 1122 949 609 176 45
PfoI T'CCnGG_A
Cuts at: 0 1043 1991 3842 4118 4127 5282 7623
Size: 1043 948 1851 276 9 1155 2341
Fragments arranged by size:
2341 1851 1155 1043 948 276 9
PpuMI rG'GwC_Cy
Cuts at: 0 428 621 1689 3267 3531 3795 4875 5019
Size: 428 193 1068 1578 264 264 1080 144
Cuts at: 5019 5424 6093 6216 6897 7623
Size: 405 669 123 681 726
Fragments arranged by size:
1578 1080 1068 726 681 669 428 405
264 264 193 144 123
PshAI GACnn'nnGTC
Cuts at: 0 527 7623
Size: 527 7096
PspOMI G'GGCC_C
Cuts at: 0 742 4853 5646 6431 7623
Size: 742 4111 793 785 1192
Fragments arranged by size:
4111 1192 793 785 742
PspXI vC'TCGA_Gb
Cuts at: 0 3803 7623
Size: 3803 3820
PstI C_TGCA'G
Cuts at: 0 1298 2037 2115 2348 2775 2802 2984 3438
Size: 1298 739 78 233 427 27 182 454
Cuts at: 3438 4011 4482 5046 5115 5235 5321 5709 5813
Size: 573 471 564 69 120 86 388 104
Cuts at: 5813 6303 6776 6939 6957 7434 7623
Size: 490 473 163 18 477 189
Fragments arranged by size:
1298 739 573 564 490 477 473 471
454 427 388 233 189 182 163 120
104 86 78 69 27 18
PvuII CAG'CTG
Cuts at: 0 121 633 1654 1921 2038 2317 2632 2680
Size: 121 512 1021 267 117 279 315 48
Cuts at: 2680 2710 3007 3057 3517 3602 4002 4012 4318
Size: 30 297 50 460 85 400 10 306
Cuts at: 4318 4523 4974 5178 5664 6112 6304 6975 7240
Size: 205 451 204 486 448 192 671 265
Cuts at: 7240 7623
Size: 383
Fragments arranged by size:
1021 671 512 486 460 451 448 400
383 315 306 297 279 267 265 205
204 192 121 117 85 50 48 30
10
RsrII CG'GwC_CG
Cuts at: 0 4116 7623
Size: 4116 3507
SacI G_AGCT'C
Cuts at: 0 3298 5849 6848 6926 7623
Size: 3298 2551 999 78 697
Fragments arranged by size:
3298 2551 999 697 78
SanDI GG'GwC_CC
Cuts at: 0 621 1689 3531 4875 7623
Size: 621 1068 1842 1344 2748
Fragments arranged by size:
2748 1842 1344 1068 621
SapI GCTCTTCn'nnn_
Cuts at: 0 7436 7623
Size: 7436 187
ScaI AGT'ACT
Cuts at: 0 5209 7623
Size: 5209 2414
SexAI A'CCwGG_T
Cuts at: 0 2504 7623
Size: 2504 5119
SfcI C'TryA_G
Cuts at: 0 1294 2033 2111 2270 2344 2771 2798 2980
Size: 1294 739 78 159 74 427 27 182
Cuts at: 2980 3190 3434 4007 4478 5042 5111 5231 5317
Size: 210 244 573 471 564 69 120 86
Cuts at: 5317 5705 5809 6299 6772 6935 6953 7430 7623
Size: 388 104 490 473 163 18 477 193
Fragments arranged by size:
1294 739 573 564 490 477 473 471
427 388 244 210 193 182 163 159
120 104 86 78 74 69 27 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 5695 7623
Size: 5695 1928
SfoI GGC'GCC
Cuts at: 0 5805 7623
Size: 5805 1818
SmaI CCC'GGG
Cuts at: 0 619 4852 4873 5161 6985 7623
Size: 619 4233 21 288 1824 638
Fragments arranged by size:
4233 1824 638 619 288 21
SmlI C'TyrA_G
Cuts at: 0 432 913 2050 2568 3212 3803 3991 5081
Size: 432 481 1137 518 644 591 188 1090
Cuts at: 5081 6385 7623
Size: 1304 1238
Fragments arranged by size:
1304 1238 1137 1090 644 591 518 481
432 188
SpeI A'CTAG_T
Cuts at: 0 2145 7107 7623
Size: 2145 4962 516
Fragments arranged by size:
4962 2145 516
SphI G_CATG'C
Cuts at: 0 1404 1509 2758 7623
Size: 1404 105 1249 4865
Fragments arranged by size:
4865 1404 1249 105
SrfI GCCC'GGGC
Cuts at: 0 4852 5161 7623
Size: 4852 309 2462
Fragments arranged by size:
4852 2462 309
SspI AAT'ATT
Cuts at: 0 379 7623
Size: 379 7244
StuI AGG'CCT
Cuts at: 0 703 1588 2830 3016 3775 5089 5929 7623
Size: 703 885 1242 186 759 1314 840 1694
Fragments arranged by size:
1694 1314 1242 885 840 759 703 186
StyI C'CwwG_G
Cuts at: 0 68 525 800 881 998 1031 2444 2621
Size: 68 457 275 81 117 33 1413 177
Cuts at: 2621 2825 3956 4586 4784 5336 5582 5772 5795
Size: 204 1131 630 198 552 246 190 23
Cuts at: 5795 6089 6353 6536 6548 6560 6995 7116 7623
Size: 294 264 183 12 12 435 121 507
Fragments arranged by size:
1413 1131 630 552 507 457 435 294
275 264 246 204 198 190 183 177
121 117 81 68 33 23 12 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2338 2437 3905 4075 5227 5389 5425 5461
Size: 2338 99 1468 170 1152 162 36 36
Cuts at: 5461 5509 5518 5938 6181 6689 7117 7623
Size: 48 9 420 243 508 428 506
Fragments arranged by size:
2338 1468 1152 508 506 428 420 243
170 162 99 48 36 36 9
TatI w'GTAC_w
Cuts at: 0 239 627 1137 2155 3450 4575 5207 6759
Size: 239 388 510 1018 1295 1125 632 1552
Cuts at: 6759 7242 7586 7623
Size: 483 344 37
Fragments arranged by size:
1552 1295 1125 1018 632 510 483 388
344 239 37
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 673 705 5174 5176 5206 5208 7113 7145
Size: 673 32 4469 2 30 2 1905 32
Cuts at: 7145 7623
Size: 478
Fragments arranged by size:
4469 1905 673 478 32 32 30 2
2
Tth111I GACn'n_nGTC
Cuts at: 0 2506 7623
Size: 2506 5117
XbaI T'CTAG_A
Cuts at: 0 3177 7623
Size: 3177 4446
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 736 2502 2604 3471 6738 7071 7623
Size: 736 1766 102 867 3267 333 552
Fragments arranged by size:
3267 1766 867 736 552 333 102
XhoI C'TCGA_G
Cuts at: 0 3212 3803 7623
Size: 3212 591 3820
Fragments arranged by size:
3820 3212 591
XmaI C'CCGG_G
Cuts at: 0 617 4850 4871 5159 6983 7623
Size: 617 4233 21 288 1824 640
Fragments arranged by size:
4233 1824 640 617 288 21
XmnI GAAnn'nnTTC
Cuts at: 0 790 7523 7623
Size: 790 6733 100
Fragments arranged by size:
6733 790 100
ZraI GAC'GTC
Cuts at: 0 3425 7623
Size: 3425 4198
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflII AflIII AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraIII
DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV
FalI FseI FspI HaeII Hin4I HincII HindIII KasI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PasI PciI PflMI PfoI PpuMI PshAI
PspOMI PspXI PstI PvuII RsrII SacI SanDI SapI
ScaI SexAI SfcI SfiI SfoI SmaI SmlI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AgeI AloI AscI AseI AsiSI AvrII
BaeI BciVI BclI BfrBI BsaBI BsiEI BsiWI BssHII
BstBI BstZ17I DraI EagI EciI EcoRI FspAI HpaI
KpnI MfeI MluI NdeI NotI NruI NsiI PacI
PmeI PmlI PpiI PsiI PsrI PvuI SacII SalI
SbfI SgfI SgrAI SnaBI SwaI