(Circular) (Six-base) MAPSORT of: pFN21ASDA0827.seq Check: 5780 from: 1 to: 9322 pFN21ASDA0827 9322 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7132 278 Size: 53 83 186 936 5596 2468 Fragments arranged by size: 5596 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4850 6665 1937 Size: 2913 1815 4594 Fragments arranged by size: 4594 2913 1815 Acc65I G'GTAC_C Cuts at: 2611 2907 5132 6643 2611 Size: 296 2225 1511 5290 Fragments arranged by size: 5290 2225 1511 296 AclI AA'CG_TT Cuts at: 1563 7449 7822 1563 Size: 5886 373 3063 Fragments arranged by size: 5886 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2214 2554 2888 4319 4434 4683 4767 4784 Size: 582 340 334 1431 115 249 84 17 Cuts at: 4784 5394 5456 5516 6014 7385 8769 9037 1632 Size: 610 62 60 498 1371 1384 268 1917 Fragments arranged by size: 1917 1431 1384 1371 610 582 498 340 334 268 249 115 84 62 60 17 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9125 AflIII A'CryG_T Cuts at: 1203 4644 8221 9198 1203 Size: 3441 3577 977 1327 Fragments arranged by size: 3577 3441 1327 977 AgeI A'CCGG_T Cuts at: 8153 8153 Size: 9322 AhdI GACnn_n'nnGTC Cuts at: 5428 8051 5428 Size: 2623 6699 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9322 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2345 3733 4518 1184 Size: 1161 1388 785 5988 Fragments arranged by size: 5988 1388 1161 785 AlwNI CAG_nnn'CTG Cuts at: 3497 3665 4100 4354 4907 7065 8637 3497 Size: 168 435 254 553 2158 1572 4182 Fragments arranged by size: 4182 2158 1572 553 435 254 168 ApaLI G'TGCA_C Cuts at: 7379 8535 7379 Size: 1156 8166 ApoI r'AATT_y Cuts at: 1493 4530 5197 6243 6325 6514 6631 6857 7009 Size: 3037 667 1046 82 189 117 226 152 Cuts at: 7009 9118 1493 Size: 2109 1697 Fragments arranged by size: 3037 2109 1697 1046 667 226 189 152 117 82 AseI AT'TA_AT Cuts at: 160 1032 7876 160 Size: 872 6844 1606 Fragments arranged by size: 6844 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9322 AvaI C'yCGr_G Cuts at: 1870 1943 6647 1870 Size: 73 4704 4545 Fragments arranged by size: 4704 4545 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2595 2628 4191 4224 6095 6128 2595 Size: 33 1563 33 1871 33 5789 Fragments arranged by size: 5789 1871 1563 33 33 33 BamHI G'GATC_C Cuts at: 6652 7019 6652 Size: 367 8955 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2611 2907 5132 6643 8098 Size: 325 217 627 824 296 2225 1511 1455 Cuts at: 8098 618 Size: 1842 Fragments arranged by size: 2225 1842 1511 1455 824 627 325 296 217 BanII G_rGCy'C Cuts at: 1391 2822 2905 5408 5672 6641 1391 Size: 1431 83 2503 264 969 4072 Fragments arranged by size: 4072 2503 1431 969 264 83 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 5205 928 Size: 971 3306 5045 Fragments arranged by size: 5045 3306 971 BbvCI CC'TCA_GC Cuts at: 2350 2350 Size: 9322 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2885 2919 7513 7547 1903 Size: 34 948 34 4594 34 3678 Fragments arranged by size: 4594 3678 948 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7214 8424 7214 Size: 1210 8112 BclI T'GATC_A Cuts at: 1548 6424 1548 Size: 4876 4446 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7933 136 Size: 107 122 71 631 605 6261 1525 Fragments arranged by size: 6261 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3617 3872 9317 3617 Size: 255 5445 3622 Fragments arranged by size: 5445 3622 255 BlpI GC'TnA_GC Cuts at: 2048 6739 2048 Size: 4691 4631 Bme1580I G_kGCm'C Cuts at: 1790 7383 8539 1790 Size: 5593 1156 2573 Fragments arranged by size: 5593 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9322 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 6043 7053 8011 Size: 416 53 83 399 24 4626 1010 958 Cuts at: 8011 442 Size: 1753 Fragments arranged by size: 4626 1753 1010 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9322 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2209 2362 5772 2209 Size: 153 3410 5759 Fragments arranged by size: 5759 3410 153 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2187 2826 3633 4452 6138 7982 1239 Size: 948 639 807 819 1686 1844 2579 Fragments arranged by size: 2579 1844 1686 948 819 807 639 Bpu10I CC'TnA_GC Cuts at: 2350 2350 Size: 9322 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5064 5271 5403 6108 7447 8312 8610 5064 Size: 207 132 705 1339 865 298 5776 Fragments arranged by size: 5776 1339 865 705 298 207 132 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4034 7985 882 Size: 617 2535 3951 2219 Fragments arranged by size: 3951 2535 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 6619 7113 493 Size: 711 5415 494 2702 Fragments arranged by size: 5415 2702 711 494 BsaBI GATnn'nnATC Cuts at: 2521 9316 2521 Size: 6795 2527 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5861 7129 7511 8146 Size: 53 83 186 936 4328 1268 382 635 Cuts at: 8146 275 Size: 1451 Fragments arranged by size: 4328 1451 1268 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 7755 8153 8427 8574 8985 7755 Size: 398 274 147 411 8092 Fragments arranged by size: 8092 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2114 2144 3631 3661 1178 Size: 30 165 30 711 30 1487 30 6839 Fragments arranged by size: 6839 1487 711 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2190 2230 2338 4884 5335 6078 6201 1186 Size: 1004 40 108 2546 451 743 123 4307 Fragments arranged by size: 4307 2546 1004 743 451 123 108 40 BseYI C'CCAG_C Cuts at: 3655 8525 9021 3655 Size: 4870 496 3956 Fragments arranged by size: 4870 3956 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4524 1163 Size: 573 2788 5961 Fragments arranged by size: 5961 2788 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6772 7533 8561 1540 Size: 275 186 4771 761 1028 2301 Fragments arranged by size: 4771 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2585 2905 7383 7468 8539 2585 Size: 320 4478 85 1071 3368 Fragments arranged by size: 4478 3368 1071 320 85 BsmI GAATG_Cn' Cuts at: 3202 6372 6833 6926 3202 Size: 3170 461 93 5598 Fragments arranged by size: 5598 3170 461 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5868 5868 Size: 9322 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2585 2822 2905 5408 5672 6641 7383 Size: 399 795 237 83 2503 264 969 742 Cuts at: 7383 7468 8539 1391 Size: 85 1071 2174 Fragments arranged by size: 2503 2174 1071 969 795 742 399 264 237 85 83 BspEI T'CCGG_A Cuts at: 8985 8985 Size: 9322 BspHI T'CATG_A Cuts at: 3279 3556 3622 7211 8941 3279 Size: 277 66 3589 1730 3660 Fragments arranged by size: 3660 3589 1730 277 66 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4930 6677 844 Size: 4086 1747 3489 Fragments arranged by size: 4086 3489 1747 BsrBI CCG'CTC Cuts at: 1394 1823 7209 1394 Size: 429 5386 3507 Fragments arranged by size: 5386 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2042 3750 4713 5388 6416 6578 7817 Size: 1183 794 1708 963 675 1028 162 1239 Cuts at: 7817 7991 65 Size: 174 1396 Fragments arranged by size: 1708 1396 1239 1183 1028 963 794 675 174 162 BsrFI r'CCGG_y Cuts at: 1700 1810 7966 8153 1700 Size: 110 6156 187 2869 Fragments arranged by size: 6156 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2448 2878 96 Size: 2352 430 6540 Fragments arranged by size: 6540 2352 430 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9322 BssSI C'ACGA_G Cuts at: 3249 7382 8394 3249 Size: 4133 1012 4177 Fragments arranged by size: 4177 4133 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3668 4100 5504 794 Size: 2874 432 1404 4612 Fragments arranged by size: 4612 2874 1404 432 BstBI TT'CG_AA Cuts at: 2007 8126 2007 Size: 6119 3203 BstEII G'GTnAC_C Cuts at: 1184 6111 1184 Size: 4927 4395 BstYI r'GATC_y Cuts at: 1972 3617 3872 6652 7019 7404 7421 8873 8959 Size: 1645 255 2780 367 385 17 1452 86 Cuts at: 8959 8971 9317 1972 Size: 12 346 1977 Fragments arranged by size: 2780 1977 1645 1452 385 367 346 255 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2466 5655 2466 Size: 3189 6133 BtgI C'CryG_G Cuts at: 513 1065 2250 2355 2745 2926 5043 5847 513 Size: 552 1185 105 390 181 2117 804 3988 Fragments arranged by size: 3988 2117 1185 804 552 390 181 105 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4140 487 Size: 683 56 2914 5669 Fragments arranged by size: 5669 2914 683 56 BtsI GCAGTG_nn' Cuts at: 3305 4402 4880 6838 7632 7652 3305 Size: 1097 478 1958 794 20 4975 Fragments arranged by size: 4975 1958 1097 794 478 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9322 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9287 DraI TTT'AAA Cuts at: 6626 6626 Size: 9322 DraIII CAC_nnn'GTG Cuts at: 2728 2728 Size: 9322 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3898 8329 809 Size: 507 2582 4431 1802 Fragments arranged by size: 4431 2582 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 6435 6769 7658 Size: 54 1425 50 275 18 4605 334 889 Cuts at: 7658 9287 8 Size: 1629 43 Fragments arranged by size: 4605 1629 1425 889 334 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6769 1537 Size: 275 4957 4090 Fragments arranged by size: 4957 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2248 2287 2426 2810 3325 3723 4606 5413 Size: 895 39 139 384 515 398 883 807 Cuts at: 5413 6149 7252 1353 Size: 736 1103 3423 Fragments arranged by size: 3423 1103 895 883 807 736 515 398 384 139 39 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7909 8283 8429 387 Size: 1064 138 6320 374 146 1280 Fragments arranged by size: 6320 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2903 2903 Size: 9322 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2187 2214 2554 2826 2888 3633 4319 Size: 393 555 27 340 272 62 745 686 Cuts at: 4319 4434 4452 4683 4767 4784 5394 5456 5516 Size: 115 18 231 84 17 610 62 60 Cuts at: 5516 6014 6138 7385 7982 8769 9037 1239 Size: 498 124 1247 597 787 268 1524 Fragments arranged by size: 1524 1247 787 745 686 610 597 555 498 393 340 272 268 231 124 115 84 62 62 60 27 18 17 EcoNI CCTnn'n_nnAGG Cuts at: 2915 5276 2915 Size: 2361 6961 EcoO109I rG'GnC_Cy Cuts at: 6041 6041 Size: 9322 EcoRI G'AATT_C Cuts at: 6243 6325 6514 6631 7009 6243 Size: 82 189 117 378 8556 Fragments arranged by size: 8556 378 189 117 82 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3152 3391 4514 5105 3152 Size: 239 1123 591 7369 Fragments arranged by size: 7369 1123 591 239 FspI TGC'GCA Cuts at: 7828 7828 Size: 9322 HaeII r_GCGC'y Cuts at: 1678 8469 1678 Size: 6791 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2024 2054 2056 2085 2086 Size: 32 135 32 452 30 2 29 1 Cuts at: 2086 2117 2546 2578 3261 3293 3464 3496 5165 Size: 31 429 32 683 32 171 32 1669 Cuts at: 5165 5197 5414 5446 7963 7995 8037 8069 1373 Size: 32 217 32 2517 32 42 32 2626 Fragments arranged by size: 2626 2517 1669 683 452 429 217 171 135 42 32 32 32 32 32 32 32 32 32 31 30 29 2 1 HincII GTy'rAC Cuts at: 669 1657 1938 2394 4408 5086 5433 5538 6666 Size: 988 281 456 2014 678 347 105 1128 Cuts at: 6666 6912 669 Size: 246 3079 Fragments arranged by size: 3079 2014 1128 988 678 456 347 281 246 105 HindIII A'AGCT_T Cuts at: 748 3373 748 Size: 2625 6697 HpaI GTT'AAC Cuts at: 2394 5086 6912 2394 Size: 2692 1826 4804 Fragments arranged by size: 4804 2692 1826 KpnI G_GTAC'C Cuts at: 2615 2911 5136 6647 2615 Size: 296 2225 1511 5290 Fragments arranged by size: 5290 2225 1511 296 MfeI C'AATT_G Cuts at: 6921 6921 Size: 9322 MluI A'CGCG_T Cuts at: 9198 9198 Size: 9322 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2468 2589 3886 4441 5593 6033 8436 8620 Size: 1163 121 1297 555 1152 440 2403 184 Cuts at: 8620 1305 Size: 2007 Fragments arranged by size: 2403 2007 1297 1163 1152 555 440 184 121 MscI TGG'CCA Cuts at: 10 64 1489 1832 6437 10 Size: 54 1425 343 4605 2895 Fragments arranged by size: 4605 2895 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4961 5393 7280 7639 7798 518 Size: 648 3795 432 1887 359 159 2042 Fragments arranged by size: 3795 2042 1887 648 432 359 159 MspA1I CmG'CkG Cuts at: 1606 2207 3665 4114 4354 4745 6738 7415 8563 Size: 601 1458 449 240 391 1993 677 1148 Cuts at: 8563 8808 1606 Size: 245 2120 Fragments arranged by size: 2120 1993 1458 1148 677 601 449 391 245 240 NcoI C'CATG_G Cuts at: 513 1065 2250 5043 5847 513 Size: 552 1185 2793 804 3988 Fragments arranged by size: 3988 2793 1185 804 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9322 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9322 NotI GC'GGCC_GC Cuts at: 6769 6769 Size: 9322 NruI TCG'CGA Cuts at: 2092 2092 Size: 9322 NspI r_CATG'y Cuts at: 1132 1229 6238 6680 8225 1132 Size: 97 5009 442 1545 2229 Fragments arranged by size: 5009 2229 1545 442 97 PasI CC'CwG_GG Cuts at: 1348 5665 1348 Size: 4317 5005 PciI A'CATG_T Cuts at: 8221 8221 Size: 9322 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 9322 PmeI GTTT'AAAC Cuts at: 6626 6626 Size: 9322 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2572 2604 7391 7423 8904 8936 2572 Size: 32 4787 32 1481 32 2958 Fragments arranged by size: 4787 2958 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 6041 6041 Size: 9322 PshAI GACnn'nnGTC Cuts at: 1613 5045 1613 Size: 3432 5890 PsiI TTA'TAA Cuts at: 6892 6892 Size: 9322 PstI C_TGCA'G Cuts at: 830 2540 3065 4903 6324 6674 830 Size: 1710 525 1838 1421 350 3478 Fragments arranged by size: 3478 1838 1710 1421 525 350 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9322 PvuII CAG'CTG Cuts at: 2207 3665 4114 4354 4745 2207 Size: 1458 449 240 391 6784 Fragments arranged by size: 6784 1458 449 391 240 SacI G_AGCT'C Cuts at: 2905 2905 Size: 9322 SalI G'TCGA_C Cuts at: 1936 6664 1936 Size: 4728 4594 SapI GCTCTTCn'nnn_ Cuts at: 2810 4606 5413 2810 Size: 1796 807 6719 Fragments arranged by size: 6719 1796 807 SbfI CC_TGCA'GG Cuts at: 6674 6674 Size: 9322 ScaI AGT'ACT Cuts at: 4381 7570 9282 4381 Size: 3189 1712 4421 Fragments arranged by size: 4421 3189 1712 SfcI C'TryA_G Cuts at: 826 1045 2154 2536 2964 2994 3061 4899 6320 Size: 219 1109 382 428 30 67 1838 1421 Cuts at: 6320 6385 6670 7805 8486 8677 826 Size: 65 285 1135 681 191 1471 Fragments arranged by size: 1838 1471 1421 1135 1109 681 428 382 285 219 191 67 65 30 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9322 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9322 SmaI CCC'GGG Cuts at: 1872 6649 1872 Size: 4777 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5079 5286 5418 6123 7426 8327 Size: 197 926 3136 207 132 705 1303 901 Cuts at: 8327 8589 820 Size: 262 1553 Fragments arranged by size: 3136 1553 1303 926 901 705 262 207 197 132 SnaBI TAC'GTA Cuts at: 493 6619 7113 493 Size: 6126 494 2702 Fragments arranged by size: 6126 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 9322 SphI G_CATG'C Cuts at: 1132 6680 1132 Size: 5548 3774 SspI AAT'ATT Cuts at: 5 52 3781 4303 7246 5 Size: 47 3729 522 2943 2081 Fragments arranged by size: 3729 2943 2081 522 47 StuI AGG'CCT Cuts at: 1517 3059 1517 Size: 1542 7780 StyI C'CwwG_G Cuts at: 513 1065 2250 4023 5043 5847 6589 6761 513 Size: 552 1185 1773 1020 804 742 172 3074 Fragments arranged by size: 3074 1773 1185 1020 804 742 552 172 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 7349 7366 7519 7704 8149 325 Size: 1255 5769 17 153 185 445 1498 Fragments arranged by size: 5769 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2448 2878 3335 Size: 274 80 33 51 1443 471 430 457 Cuts at: 3335 4379 7568 9280 96 Size: 1044 3189 1712 138 Fragments arranged by size: 3189 1712 1443 1044 471 457 430 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2495 2527 3631 3663 9282 9314 2495 Size: 32 1104 32 5619 32 2503 Fragments arranged by size: 5619 2503 1104 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4412 1316 Size: 3096 6226 XbaI T'CTAG_A Cuts at: 2141 4175 6658 2141 Size: 2034 2483 4805 Fragments arranged by size: 4805 2483 2034 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2801 5854 2801 Size: 3053 6269 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9322 XmaI C'CCGG_G Cuts at: 1870 6647 1870 Size: 4777 4545 XmnI GAAnn'nnTTC Cuts at: 3516 4558 4623 7451 3516 Size: 1042 65 2828 5387 Fragments arranged by size: 5387 2828 1042 65 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7130 276 Size: 53 83 186 936 5596 2468 Fragments arranged by size: 5596 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BsiWI BstXI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI