Restriction map for
pFN21ASDA0742
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AcyI AgeI AhdI
Alw44I Aor51HI ApaLI AscI Asp718I
AspEI AspI AviII AvrII BanI
BbeI BbrPI BbvCI BcgI BciVI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsiEI BsiWI BsmBI
BspDI BsrBI BsrDI BssHII BssSI
Bst1107I BstAPI BstBI BstZ17I CelII
ClaI CpoI DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoT22I
FseI FspI HaeII Hin1I HpaI
KasI KpnI KspI MamI MluI
NarI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 109 CGCG CG^CG
AccIII 2654 TCCGGA T^CCGGA
AclI 1825 AACGTT AA^CGTT
AflIII 748 ACATGT A^C[GA][CT]GT
AlwNI 2384 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1240 CCTAAGG CC^T[ACGT]AGG
ApaI 3288 GGGCCC GGGCC^C
AseI 3315 ATTAAT AT^TAAT
AsnI 3315 ATTAAT AT^TAAT
Asp700I 3082 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 3214 CCCGAG C^[CT]CG[GA]G
BamHI 376 GGATCC G^GATCC
BglII 3282 AGATCT A^GATCT
BsaI 1877 GAGACC GGTCTC(1/5)
BseAI 2654 TCCGGA T^CCGGA
BsoBI 3214 CCCGAG C^[CT]CG[GA]G
BspEI 2654 TCCGGA T^CCGGA
BspHI 3621 TCATGA T^CATGA
BspLU11I 748 ACATGT A^CATGT
BstEII 1808 GGTTACC G^GT[ACGT]ACC
BstPI 1808 GGTTACC G^GT[ACGT]ACC
BstUI 109 CGCG CG^CG
BstXI 2801 CCAGCATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1240 CCTAAGG CC^T[ACGT]AGG
CfoI 108 GCGC GCG^C
DraIII 2017 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1240 CCTAAGG CC^T[ACGT]AGG
EcoNI 1236 CCTTCCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1808 GGTTACC G^GT[ACGT]ACC
EcoRV 3842 GATATC GAT^ATC
EcoT14I 984 CCTTGG C^C[AT][AT]GG
HgaI 110 GCGTC GACGC(5/10)
HhaI 108 GCGC GCG^C
HinP1I 108 GCGC G^CGC
HindIII 512 AAGCTT A^AGCTT
MfeI 1796 CAATTG C^AATTG
MroI 2654 TCCGGA T^CCGGA
MunI 1796 CAATTG C^AATTG
MvnI 109 CGCG CG^CG
NaeI 2419 GCCGGC GCC^GGC
NgoMIV 2419 GCCGGC G^CCGGC
PpuMI 935 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 3315 ATTAAT AT^TAAT
Psp1406I 1825 AACGTT AA^CGTT
RcaI 3621 TCATGA T^CATGA
SacI 3737 GAGCTC GAGCT^C
SapI 3580 GAAGAGC GCTCTTC(1/4)
ScaI 952 AGTACT AGT^ACT
StyI 984 CCTTGG C^C[AT][AT]GG
XmnI 3082 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AccI 238 GTCTAC GT^[AC][GT]AC
3010 GTAGAC
AflII 3133 CTTAAG C^TTAAG
3439 CTTAAG
BanII 3288 GGGCCC G[GA]GC[CT]^C
3737 GAGCTC
BclI 1191 TGATCA T^GATCA
3624 TGATCA
BcnI 134 CCGGG CC^[GC]GG
3729 CCCGG
BfrI 3133 CTTAAG C^TTAAG
3439 CTTAAG
BsaWI 2063 ACCGGA [AT]^CCGG[AT]
2654 TCCGGA
BsmFI 98 GGGAC GGGAC(10/14)
136 GGGAC
BsmI 1515 GAATGC GAATGC(1/-1)
2804 GCATTC
BspMI 2012 ACCTGC ACCTGC(4/8)
1369 GCAGGT
BsrFI 27 GCCGGT [GA]^CCGG[CT]
2419 GCCGGC
Cfr10I 27 GCCGGT [GA]^CCGG[CT]
2419 GCCGGC
DraII 935 AGGACCC [GA]G^G[ACGT]CC[CT]
3288 GGGCCCC
EcoO109I 935 AGGACCC [GA]G^G[ACGT]CC[CT]
3288 GGGCCCC
FauI 1752 GCGGG CCCGC(4/6)
2486 GCGGG
FbaI 1191 TGATCA T^GATCA
3624 TGATCA
NciI 134 CCGGG CC^[GC]GG
3729 CCCGG
PstI 914 CTGCAG CTGCA^G
1306 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BalI BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI Bsp1286I Bsp1407I BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
EaeI EarI EcoRI EcoRII Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI StuI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII