Restriction map for
pFN21ASDA0587
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII Acc65I AccIII AclI AgeI
AhdI AocI Aor51HI ApaI AscI
Asp700I Asp718I AspEI AvaI AviII
AvrII BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BglI BlnI
BlpI Bpu1102I BsaWI BseAI BseRI
BsiWI BsmBI BsoBI BspDI BspEI
BspHI BspMI BsrBI BsrFI BssHII
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraIII EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV FseI FspI HindIII
KasI KpnI KspI MluI MroI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI Sse8387I
StuI SwaI XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 5 CGCG CG^CG
AcyI 70 GGCGTC G[GA]^CG[CT]C
AflII 2614 CTTAAG C^TTAAG
AflIII 1269 ACATGT A^C[GA][CT]GT
Alw44I 719 GTGCAC G^TGCAC
AlwNI 31 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 719 GTGCAC G^TGCAC
AspI 2719 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 830 GGATCC G^GATCC
BclI 1092 TGATCA T^GATCA
BcnI 66 CCGGG CC^[GC]GG
BfrI 2614 CTTAAG C^TTAAG
BsaAI 1306 TACGTG [CT]AC^GT[GA]
BsaBI 2705 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 70 GGCGTC G[GA]^CG[CT]C
BsaI 134 GAGACC GGTCTC(1/5)
BsiEI 63 CGACCG CG[GA][CT]^CG
BsmFI 1112 GTCCC GGGAC(10/14)
BsmI 994 GAATGC GAATGC(1/-1)
Bsp1407I 3291 TGTACA T^GTACA
BspLU11I 1269 ACATGT A^CATGT
BsrGI 3291 TGTACA T^GTACA
BstAPI 3361 GCATACCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 5 CGCG CG^CG
DraII 1110 AGGTCCC [GA]G^G[ACGT]CC[CT]
DrdI 64 GACCGGGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 825 CCATGG C^C[GA][CT]GG
EcoO109I 1110 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoT22I 3359 ATGCAT ATGCA^T
FbaI 1092 TGATCA T^GATCA
HaeII 3195 GGCGCT [GA]GCGC^[CT]
Hin1I 70 GGCGTC G[GA]^CG[CT]C
MamI 2705 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 334 CATGTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 5 CGCG CG^CG
NciI 66 CCGGG CC^[GC]GG
NcoI 825 CCATGG C^CATGG
NdeI 2552 CATATG CA^TATG
NsiI 3359 ATGCAT ATGCA^T
PflFI 2719 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 544 CCACGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 516 GACTC GAGTC(4/5)
PpuMI 1110 AGGTCCC [GA]G^G[AT]CC[CT]
SexAI 1995 ACCAGGT A^CC[AT]GGT
SmlI 2614 CTTAAG C^T[CT][GA]AG
SpeI 1507 ACTAGT A^CTAGT
SphI 77 GCATGC GCATG^C
SspBI 3291 TGTACA T^GTACA
Tth111I 2719 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 544 CCACGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccI 3212 GTCTAC GT^[AC][GT]AC
3373 GTCTAC
AseI 979 ATTAAT AT^TAAT
1239 ATTAAT
AsnI 979 ATTAAT AT^TAAT
1239 ATTAAT
AspHI 719 GTGCAC G[AT]GC[AT]^C
1265 GAGCAC
BglII 1057 AGATCT A^GATCT
1629 AGATCT
BmyI 719 GTGCAC G[AGT]GC[ACT]^C
1265 GAGCAC
BpmI 2266 CTCCAG CTGGAG(16/14)
3207 CTCCAG
BsiHKAI 719 GTGCAC G[AT]GC[AT]^C
1265 GAGCAC
Bsp1286I 719 GTGCAC G[AGT]GC[ACT]^C
1265 GAGCAC
BssSI 2129 CACGAG CACGAG(-5/-1)
1351 CTCGTG
CfoI 6 GCGC GCG^C
3196 GCGC
DraI 582 TTTAAA TTT^AAA
2698 TTTAAA
FauI 86 CCCGC CCCGC(4/6)
224 CCCGC
HapII 66 CCGG C^CGG
2912 CCGG
HhaI 6 GCGC GCG^C
3196 GCGC
HinP1I 6 GCGC G^CGC
3196 GCGC
HpaI 1408 GTTAAC GTT^AAC
2646 GTTAAC
HpaII 66 CCGG C^CGG
2912 CCGG
HphI 50 TCACC GGTGA(8/7)
83 TCACC
MspA1I 677 CAGCTG C[AC]G^C[GT]G
2906 CAGCTG
MspI 66 CCGG C^CGG
2912 CCGG
PshBI 979 ATTAAT AT^TAAT
1239 ATTAAT
PvuII 677 CAGCTG CAG^CTG
2906 CAGCTG
SapI 1774 GAAGAGC GCTCTTC(1/4)
874 GCTCTTC
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# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BalI BbsI BbvI
BfaI BpuAI BsaJI BsgI BsiYI
BslI BsmAI BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII EaeI EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HgaI
HincII HindII HinfI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MfeI MflI MluNI MnlI MscI
MseI MunI MvaI MwoI NdeII
NlaIII NlaIV NspI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII