(Circular) (Six-base) MAPSORT of: pFN21ASDA0587.seq Check: 2283 from: 1 to: 8131 pFN21ASDA0587 8131 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5941 278 Size: 53 83 186 936 4405 2468 Fragments arranged by size: 4405 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5236 5397 5474 1937 Size: 3299 161 77 4594 Fragments arranged by size: 4594 3299 161 77 Acc65I G'GTAC_C Cuts at: 5452 5452 Size: 8131 AclI AA'CG_TT Cuts at: 1563 6258 6631 1563 Size: 4695 373 3063 Fragments arranged by size: 4695 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3110 3420 4233 6194 7578 7846 1632 Size: 1478 310 813 1961 1384 268 1917 Fragments arranged by size: 1961 1917 1478 1384 813 310 268 AflII C'TTAA_G Cuts at: 820 1017 4637 820 Size: 197 3620 4314 Fragments arranged by size: 4314 3620 197 AflIII A'CryG_T Cuts at: 1203 3292 7030 8007 1203 Size: 2089 3738 977 1327 Fragments arranged by size: 3738 2089 1327 977 AgeI A'CCGG_T Cuts at: 6962 6962 Size: 8131 AhdI GACnn_n'nnGTC Cuts at: 6860 6860 Size: 8131 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8131 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8131 AlwNI CAG_nnn'CTG Cuts at: 2059 5874 7446 2059 Size: 3815 1572 2744 Fragments arranged by size: 3815 2744 1572 ApaLI G'TGCA_C Cuts at: 2742 6188 7344 2742 Size: 3446 1156 3529 Fragments arranged by size: 3529 3446 1156 ApoI r'AATT_y Cuts at: 1493 2213 2228 2345 2624 5210 5440 5666 5818 Size: 720 15 117 279 2586 230 226 152 Cuts at: 5818 7927 1493 Size: 2109 1697 Fragments arranged by size: 2586 2109 1697 720 279 230 226 152 117 15 AseI AT'TA_AT Cuts at: 160 1032 3003 3263 6685 160 Size: 872 1971 260 3422 1606 Fragments arranged by size: 3422 1971 1606 872 260 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8131 AvaI C'yCGr_G Cuts at: 1870 1943 5456 1870 Size: 73 3513 4545 Fragments arranged by size: 4545 3513 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2465 2498 2465 Size: 33 8098 BamHI G'GATC_C Cuts at: 2853 5461 5828 2853 Size: 2608 367 5156 Fragments arranged by size: 5156 2608 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5452 6907 618 Size: 325 217 627 3665 1455 1842 Fragments arranged by size: 3665 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 5450 1391 Size: 4059 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2389 2970 5202 928 Size: 971 490 581 2232 3857 Fragments arranged by size: 3857 2232 971 581 490 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6322 6356 1903 Size: 34 4385 34 3678 Fragments arranged by size: 4385 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6023 7233 6023 Size: 1210 6921 BclI T'GATC_A Cuts at: 1548 3115 1548 Size: 1567 6564 BfrBI ATG'CAT Cuts at: 5384 5384 Size: 8131 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6742 136 Size: 107 122 71 631 605 5070 1525 Fragments arranged by size: 5070 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3080 3652 8126 3080 Size: 572 4474 3085 Fragments arranged by size: 4474 3085 572 BlpI GC'TnA_GC Cuts at: 5548 5548 Size: 8131 Bme1580I G_kGCm'C Cuts at: 1790 2746 6192 7348 1790 Size: 956 3446 1156 2573 Fragments arranged by size: 3446 2573 1156 956 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8131 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2040 2716 5862 Size: 416 53 83 399 24 623 676 3146 Cuts at: 5862 6820 442 Size: 958 1753 Fragments arranged by size: 3146 1753 958 676 623 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8131 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3736 3736 Size: 8131 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4274 5215 6791 1239 Size: 3035 941 1576 2579 Fragments arranged by size: 3035 2579 1576 941 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6256 7121 7419 6256 Size: 865 298 6968 Fragments arranged by size: 6968 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2151 6794 882 Size: 617 652 4643 2219 Fragments arranged by size: 4643 2219 652 617 BsaAI yAC'GTr Cuts at: 493 1204 3331 5428 5922 493 Size: 711 2127 2097 494 2702 Fragments arranged by size: 2702 2127 2097 711 494 BsaBI GATnn'nnATC Cuts at: 4732 8125 4732 Size: 3393 4738 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2094 5938 6320 6955 Size: 53 83 186 936 561 3844 382 635 Cuts at: 6955 275 Size: 1451 Fragments arranged by size: 3844 1451 936 635 561 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6564 6962 7236 7383 7794 6564 Size: 398 274 147 411 6901 Fragments arranged by size: 6901 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7906 Fragments arranged by size: 7906 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 8131 BseYI C'CCAG_C Cuts at: 7334 7830 7334 Size: 496 7635 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3069 3249 3353 3398 4065 4331 4698 Size: 573 1333 180 104 45 667 266 367 Cuts at: 4698 1163 Size: 4596 Fragments arranged by size: 4596 1333 667 573 367 266 180 104 45 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2089 5581 6342 7370 1540 Size: 275 186 88 3492 761 1028 2301 Fragments arranged by size: 3492 2301 1028 761 275 186 88 BsiHKAI G_wGCw'C Cuts at: 2746 3292 6192 6277 7348 2746 Size: 546 2900 85 1071 3529 Fragments arranged by size: 3529 2900 1071 546 85 BsmI GAATG_Cn' Cuts at: 3023 5642 5735 3023 Size: 2619 93 5419 Fragments arranged by size: 5419 2619 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2746 3292 5450 6192 6277 7348 1391 Size: 399 956 546 2158 742 85 1071 2174 Fragments arranged by size: 2174 2158 1071 956 742 546 399 85 BspEI T'CCGG_A Cuts at: 7794 7794 Size: 8131 BspHI T'CATG_A Cuts at: 6020 7750 6020 Size: 1730 6401 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5486 844 Size: 4642 3489 BsrBI CCG'CTC Cuts at: 1394 1823 6018 1394 Size: 429 4195 3507 Fragments arranged by size: 4195 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2817 3252 4450 4476 4911 5113 5128 Size: 1183 1569 435 1198 26 435 202 15 Cuts at: 5128 6626 6800 65 Size: 1498 174 1396 Fragments arranged by size: 1569 1498 1396 1198 1183 435 435 202 174 26 15 BsrFI r'CCGG_y Cuts at: 1700 1810 6775 6962 1700 Size: 110 4965 187 2869 Fragments arranged by size: 4965 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 5314 96 Size: 5218 2913 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8131 BssSI C'ACGA_G Cuts at: 3374 4152 6191 7203 3374 Size: 778 2039 1012 4302 Fragments arranged by size: 4302 2039 1012 778 BstAPI GCAn_nnn'nTGC Cuts at: 794 5390 794 Size: 4596 3535 BstBI TT'CG_AA Cuts at: 2007 6935 2007 Size: 4928 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8131 BstYI r'GATC_y Cuts at: 1972 2853 3080 3652 5341 5461 5828 6213 6230 Size: 881 227 572 1689 120 367 385 17 Cuts at: 6230 7682 7768 7780 8126 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1689 1452 881 572 385 367 346 227 120 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2848 513 Size: 552 1783 5796 Fragments arranged by size: 5796 1783 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7392 Fragments arranged by size: 7392 683 56 BtsI GCAGTG_nn' Cuts at: 3966 4077 5647 6441 6461 3966 Size: 111 1570 794 20 5636 Fragments arranged by size: 5636 1570 794 111 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8131 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4107 4142 529 Size: 35 3543 35 4518 Fragments arranged by size: 4518 3543 35 35 DraI TTT'AAA Cuts at: 2607 4723 5435 2607 Size: 2116 712 5303 Fragments arranged by size: 5303 2116 712 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2093 7138 809 Size: 507 777 5045 1802 Fragments arranged by size: 5045 1802 777 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2575 3858 4447 Size: 54 1425 50 275 18 745 1283 589 Cuts at: 4447 5070 5136 5248 5578 6467 8096 8 Size: 623 66 112 330 889 1629 43 Fragments arranged by size: 1629 1425 1283 889 745 623 589 330 275 112 66 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5578 1537 Size: 275 3766 4090 Fragments arranged by size: 4090 3766 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2673 2904 3218 3792 4748 5007 6061 1353 Size: 1320 231 314 574 956 259 1054 3423 Fragments arranged by size: 3423 1320 1054 956 574 314 259 231 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2078 6718 7092 7238 387 Size: 1064 138 489 4640 374 146 1280 Fragments arranged by size: 4640 1280 1064 489 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3110 3420 4233 4274 5215 6194 6791 Size: 393 1478 310 813 41 941 979 597 Cuts at: 6791 7578 7846 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1478 979 941 813 787 597 393 310 268 41 EcoO109I rG'GnC_Cy Cuts at: 3134 3134 Size: 8131 EcoRI G'AATT_C Cuts at: 5440 5818 5440 Size: 378 7753 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2455 2928 4826 5219 2455 Size: 473 1898 393 5367 Fragments arranged by size: 5367 1898 473 393 FspI TGC'GCA Cuts at: 6637 6637 Size: 8131 HaeII r_GCGC'y Cuts at: 1678 5222 7278 1678 Size: 3544 2056 2531 Fragments arranged by size: 3544 2531 2056 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4156 4188 4808 4840 4910 Size: 32 135 32 2584 32 620 32 70 Cuts at: 4910 4942 6772 6804 6846 6878 1373 Size: 32 1830 32 42 32 2626 Fragments arranged by size: 2626 2584 1830 620 135 70 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3433 4671 4741 5475 5721 669 Size: 988 281 1495 1238 70 734 246 3079 Fragments arranged by size: 3079 1495 1238 988 734 281 246 70 HindIII A'AGCT_T Cuts at: 748 748 Size: 8131 HpaI GTT'AAC Cuts at: 3433 4671 5721 3433 Size: 1238 1050 5843 Fragments arranged by size: 5843 1238 1050 KpnI G_GTAC'C Cuts at: 5456 5456 Size: 8131 MfeI C'AATT_G Cuts at: 3738 4197 4854 5730 3738 Size: 459 657 876 6139 Fragments arranged by size: 6139 876 657 459 MluI A'CGCG_T Cuts at: 8007 8007 Size: 8131 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2799 7245 7429 1305 Size: 1494 4446 184 2007 Fragments arranged by size: 4446 2007 1494 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2577 3860 4449 5072 5138 Size: 54 1425 343 745 1283 589 623 66 Cuts at: 5138 5250 10 Size: 112 2891 Fragments arranged by size: 2891 1425 1283 745 623 589 343 112 66 54 MslI CAynn'nnrTG Cuts at: 518 1166 2361 6089 6448 6607 518 Size: 648 1195 3728 359 159 2042 Fragments arranged by size: 3728 2042 1195 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2702 4931 5547 6224 7372 7617 1606 Size: 1096 2229 616 677 1148 245 2120 Fragments arranged by size: 2229 2120 1148 1096 677 616 245 NcoI C'CATG_G Cuts at: 513 1065 2848 513 Size: 552 1783 5796 Fragments arranged by size: 5796 1783 552 NdeI CA'TA_TG Cuts at: 387 4576 387 Size: 4189 3942 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8131 NotI GC'GGCC_GC Cuts at: 5578 5578 Size: 8131 NsiI A_TGCA'T Cuts at: 5386 5386 Size: 8131 NspI r_CATG'y Cuts at: 1132 1229 2104 2154 3044 3217 3296 5489 7034 Size: 97 875 50 890 173 79 2193 1545 Cuts at: 7034 1132 Size: 2229 Fragments arranged by size: 2229 2193 1545 890 875 173 97 79 50 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8131 PciI A'CATG_T Cuts at: 3292 7030 3292 Size: 3738 4393 PflMI CCAn_nnn'nTGG Cuts at: 1108 2573 1108 Size: 1465 6666 PmeI GTTT'AAAC Cuts at: 5435 5435 Size: 8131 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6200 6232 7713 7745 6200 Size: 32 1481 32 6586 Fragments arranged by size: 6586 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3134 3134 Size: 8131 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8131 PsiI TTA'TAA Cuts at: 2453 5701 2453 Size: 3248 4883 PstI C_TGCA'G Cuts at: 830 2043 2962 5164 5483 830 Size: 1213 919 2202 319 3478 Fragments arranged by size: 3478 2202 1213 919 319 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8131 PvuII CAG'CTG Cuts at: 2702 4931 2702 Size: 2229 5902 SalI G'TCGA_C Cuts at: 1936 5473 1936 Size: 3537 4594 SapI GCTCTTCn'nnn_ Cuts at: 2904 3792 2904 Size: 888 7243 SbfI CC_TGCA'GG Cuts at: 5483 5483 Size: 8131 ScaI AGT'ACT Cuts at: 6379 8091 6379 Size: 1712 6419 SexAI A'CCwGG_T Cuts at: 4018 4018 Size: 8131 SfcI C'TryA_G Cuts at: 826 1045 2039 2238 2276 2871 2958 4494 5103 Size: 219 994 199 38 595 87 1536 609 Cuts at: 5103 5160 5479 6614 7295 7486 826 Size: 57 319 1135 681 191 1471 Fragments arranged by size: 1536 1471 1135 994 681 609 595 319 219 199 191 87 57 38 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8131 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8131 SmaI CCC'GGG Cuts at: 1872 5458 1872 Size: 3586 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4637 6235 7136 7398 820 Size: 197 926 2694 1598 901 262 1553 Fragments arranged by size: 2694 1598 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5428 5922 493 Size: 4935 494 2702 Fragments arranged by size: 4935 2702 494 SpeI A'CTAG_T Cuts at: 152 3530 152 Size: 3378 4753 SphI G_CATG'C Cuts at: 1132 2104 5489 1132 Size: 972 3385 3774 Fragments arranged by size: 3774 3385 972 SspI AAT'ATT Cuts at: 5 52 3267 4160 4529 6055 5 Size: 47 3215 893 369 1526 2081 Fragments arranged by size: 3215 2081 1526 893 369 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8131 StyI C'CwwG_G Cuts at: 513 1065 2163 2572 2848 3138 3624 4880 5570 Size: 552 1098 409 276 290 486 1256 690 Cuts at: 5570 513 Size: 3074 Fragments arranged by size: 3074 1256 1098 690 552 486 409 290 276 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 6158 6175 6328 6513 6958 325 Size: 1255 4578 17 153 185 445 1498 Fragments arranged by size: 4578 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2750 5091 5314 Size: 274 80 33 51 1443 773 2341 223 Cuts at: 5314 6377 8089 96 Size: 1063 1712 138 Fragments arranged by size: 2341 1712 1443 1063 773 274 223 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2137 2169 8091 8123 2137 Size: 32 5922 32 2145 Fragments arranged by size: 5922 2145 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4745 1316 Size: 3429 4702 XbaI T'CTAG_A Cuts at: 5467 5467 Size: 8131 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8131 XmaI C'CCGG_G Cuts at: 1870 5456 1870 Size: 3586 4545 XmnI GAAnn'nnTTC Cuts at: 6260 6260 Size: 8131 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5939 276 Size: 53 83 186 936 4405 2468 Fragments arranged by size: 4405 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI