Restriction map for
pFN21ASDA0587
(Linear) (Six-base) MAPSORT of: KIAA0587.seq Check: 4801 from: 1 to: 3402
KIAA0587.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AccI GT'mk_AC
Cuts at: 0 3213 3374 3402
Size: 3213 161 28
Fragments arranged by size:
3213 161 28
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1087 1397 2210 3402
Size: 1087 310 813 1192
Fragments arranged by size:
1192 1087 813 310
AflII C'TTAA_G
Cuts at: 0 2614 3402
Size: 2614 788
AflIII A'CryG_T
Cuts at: 0 1269 3402
Size: 1269 2133
AlwNI CAG_nnn'CTG
Cuts at: 0 36 3402
Size: 36 3366
ApaLI G'TGCA_C
Cuts at: 0 719 3402
Size: 719 2683
ApoI r'AATT_y
Cuts at: 0 190 205 322 601 3187 3402
Size: 190 15 117 279 2586 215
Fragments arranged by size:
2586 279 215 190 117 15
AseI AT'TA_AT
Cuts at: 0 980 1240 3402
Size: 980 260 2162
Fragments arranged by size:
2162 980 260
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 442 475 3402
Size: 442 33 2927
Fragments arranged by size:
2927 442 33
BamHI G'GATC_C
Cuts at: 0 830 3402
Size: 830 2572
BbsI GAAGACnn'nnnn_
Cuts at: 0 366 947 3179 3402
Size: 366 581 2232 223
Fragments arranged by size:
2232 581 366 223
BclI T'GATC_A
Cuts at: 0 1092 3402
Size: 1092 2310
BfrBI ATG'CAT
Cuts at: 0 3361 3402
Size: 3361 41
BglII A'GATC_T
Cuts at: 0 1057 1629 3402
Size: 1057 572 1773
Fragments arranged by size:
1773 1057 572
Bme1580I G_kGCm'C
Cuts at: 0 723 3402
Size: 723 2679
BmrI ACTGGGnnnn_n'
Cuts at: 0 17 693 3402
Size: 17 676 2709
Fragments arranged by size:
2709 676 17
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1713 3402
Size: 1713 1689
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2251 3192 3402
Size: 2251 941 210
Fragments arranged by size:
2251 941 210
BsaI GGTCTCn'nnnn_
Cuts at: 0 128 3402
Size: 128 3274
BsaAI yAC'GTr
Cuts at: 0 1308 3402
Size: 1308 2094
BsaBI GATnn'nnATC
Cuts at: 0 2709 3402
Size: 2709 693
BsaHI Gr'CG_yC
Cuts at: 0 71 3402
Size: 71 3331
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1046 1226 1330 1375 2042 2308 2675 3402
Size: 1046 180 104 45 667 266 367 727
Fragments arranged by size:
1046 727 667 367 266 180 104 45
BsiEI CG_ry'CG
Cuts at: 0 66 3402
Size: 66 3336
BsiHKAI G_wGCw'C
Cuts at: 0 723 1269 3402
Size: 723 546 2133
Fragments arranged by size:
2133 723 546
BsmI GAATG_Cn'
Cuts at: 0 1000 3402
Size: 1000 2402
Bsp1286I G_dGCh'C
Cuts at: 0 723 1269 3402
Size: 723 546 2133
Fragments arranged by size:
2133 723 546
BsrDI GCAATG_nn'
Cuts at: 0 794 1229 2427 2453 2888 3090 3105 3402
Size: 794 435 1198 26 435 202 15 297
Fragments arranged by size:
1198 794 435 435 297 202 26 15
BsrGI T'GTAC_A
Cuts at: 0 3291 3402
Size: 3291 111
BssSI C'ACGA_G
Cuts at: 0 1351 2129 3402
Size: 1351 778 1273
Fragments arranged by size:
1351 1273 778
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3367 3402
Size: 3367 35
BstYI r'GATC_y
Cuts at: 0 830 1057 1629 3318 3402
Size: 830 227 572 1689 84
Fragments arranged by size:
1689 830 572 227 84
BtgI C'CryG_G
Cuts at: 0 825 3402
Size: 825 2577
BtsI GCAGTG_nn'
Cuts at: 0 1943 2054 3402
Size: 1943 111 1348
Fragments arranged by size:
1943 1348 111
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2084 2119 3402
Size: 2084 35 1283
Fragments arranged by size:
2084 1283 35
DraI TTT'AAA
Cuts at: 0 584 2700 3402
Size: 584 2116 702
Fragments arranged by size:
2116 702 584
DrdI GACnn_nn'nnGTC
Cuts at: 0 70 3402
Size: 70 3332
EaeI y'GGCC_r
Cuts at: 0 552 1835 2424 3047 3113 3225 3402
Size: 552 1283 589 623 66 112 177
Fragments arranged by size:
1283 623 589 552 177 112 66
EarI CTCTTCn'nnn_
Cuts at: 0 650 881 1195 1769 2725 2984 3402
Size: 650 231 314 574 956 259 418
Fragments arranged by size:
956 650 574 418 314 259 231
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 55 3402
Size: 55 3347
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1087 1397 2210 2251 3192 3402
Size: 1087 310 813 41 941 210
Fragments arranged by size:
1087 941 813 310 210 41
EcoO109I rG'GnC_Cy
Cuts at: 0 1111 3402
Size: 1111 2291
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 432 905 2803 3196 3402
Size: 432 473 1898 393 206
Fragments arranged by size:
1898 473 432 393 206
HaeII r_GCGC'y
Cuts at: 0 3199 3402
Size: 3199 203
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2133 2165 2785 2817 2887 2919 3402
Size: 2133 32 620 32 70 32 483
Fragments arranged by size:
2133 620 483 70 32 32 32
HincII GTy'rAC
Cuts at: 0 1410 2648 2718 3402
Size: 1410 1238 70 684
Fragments arranged by size:
1410 1238 684 70
HpaI GTT'AAC
Cuts at: 0 1410 2648 3402
Size: 1410 1238 754
Fragments arranged by size:
1410 1238 754
MfeI C'AATT_G
Cuts at: 0 1715 2174 2831 3402
Size: 1715 459 657 571
Fragments arranged by size:
1715 657 571 459
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 776 3402
Size: 776 2626
MscI TGG'CCA
Cuts at: 0 554 1837 2426 3049 3115 3227 3402
Size: 554 1283 589 623 66 112 175
Fragments arranged by size:
1283 623 589 554 175 112 66
MslI CAynn'nnrTG
Cuts at: 0 338 3402
Size: 338 3064
MspA1I CmG'CkG
Cuts at: 0 679 2908 3402
Size: 679 2229 494
Fragments arranged by size:
2229 679 494
NcoI C'CATG_G
Cuts at: 0 825 3402
Size: 825 2577
NdeI CA'TA_TG
Cuts at: 0 2553 3402
Size: 2553 849
NsiI A_TGCA'T
Cuts at: 0 3363 3402
Size: 3363 39
NspI r_CATG'y
Cuts at: 0 81 131 1021 1194 1273 3402
Size: 81 50 890 173 79 2129
Fragments arranged by size:
2129 890 173 81 79 50
PciI A'CATG_T
Cuts at: 0 1269 3402
Size: 1269 2133
PflMI CCAn_nnn'nTGG
Cuts at: 0 550 3402
Size: 550 2852
PpuMI rG'GwC_Cy
Cuts at: 0 1111 3402
Size: 1111 2291
PsiI TTA'TAA
Cuts at: 0 430 3402
Size: 430 2972
PstI C_TGCA'G
Cuts at: 0 20 939 3141 3402
Size: 20 919 2202 261
Fragments arranged by size:
2202 919 261 20
PvuII CAG'CTG
Cuts at: 0 679 2908 3402
Size: 679 2229 494
Fragments arranged by size:
2229 679 494
SapI GCTCTTCn'nnn_
Cuts at: 0 881 1769 3402
Size: 881 888 1633
Fragments arranged by size:
1633 888 881
SexAI A'CCwGG_T
Cuts at: 0 1995 3402
Size: 1995 1407
SfcI C'TryA_G
Cuts at: 0 16 215 253 848 935 2471 3080 3137
Size: 16 199 38 595 87 1536 609 57
Cuts at: 3137 3402
Size: 265
Fragments arranged by size:
1536 609 595 265 199 87 57 38
16
SmlI C'TyrA_G
Cuts at: 0 2614 3402
Size: 2614 788
SpeI A'CTAG_T
Cuts at: 0 1507 3402
Size: 1507 1895
SphI G_CATG'C
Cuts at: 0 81 3402
Size: 81 3321
SspI AAT'ATT
Cuts at: 0 1244 2137 2506 3402
Size: 1244 893 369 896
Fragments arranged by size:
1244 896 893 369
StyI C'CwwG_G
Cuts at: 0 140 549 825 1115 1601 2857 3402
Size: 140 409 276 290 486 1256 545
Fragments arranged by size:
1256 545 486 409 290 276 140
TatI w'GTAC_w
Cuts at: 0 727 3068 3291 3402
Size: 727 2341 223 111
Fragments arranged by size:
2341 727 223 111
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 114 146 3402
Size: 114 32 3256
Fragments arranged by size:
3256 114 32
Tth111I GACn'n_nGTC
Cuts at: 0 2722 3402
Size: 2722 680
Enzymes that do cut:
AccI AcuI AflII AflIII AlwNI ApaLI ApoI AseI
BaeI BamHI BbsI BclI BfrBI BglII Bme1580I BmrI
BplI BpmI BsaI BsaAI BsaBI BsaHI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BsrDI BsrGI BssSI BstAPI BstYI
BtgI BtsI CspCI DraI DrdI EaeI EarI EciI
Eco57MI EcoO109I FalI HaeII Hin4I HincII HpaI MfeI
MmeI MscI MslI MspA1I NcoI NdeI NsiI NspI
PciI PflMI PpuMI PsiI PstI PvuII SapI SexAI
SfcI SmlI SpeI SphI SspI StyI TatI TstI
Tth111I
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AleI
AlfI AloI ApaI AscI AsiSI AvaI AvrII BanI
BanII BbeI BbvCI BcgI BciVI BglI BlpI BmgBI
BmtI Bpu10I BpuEI BsaWI BsaXI BseRI BseYI BsiWI
BsmBI BspEI BspHI BspMI BsrBI BsrFI BssHII BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI ClaI DraIII EagI
EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HindIII
KasI KpnI MluI NaeI NarI NgoMIV NheI NotI
NruI PacI PasI PfoI PmeI PmlI PpiI PshAI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SrfI StuI SwaI TaqII XbaI XcmI XhoI
XmaI XmnI ZraI