Restriction map for
pFN21AB7804
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Alw44I AocI ApaI ApaLI
AscI AseI AsnI AspEI AspI
AviII AvrII BamHI BbsI BbvCI
BcgI BfaI BfrI BglII BlnI
BpuAI BsaBI BsaWI BseAI BsiWI
BsmBI BspEI BspHI BssHII Bst1107I
BstAPI BstBI BstZ17I Bsu36I CpoI
DraI DraIII DrdI Eam1105I Eco81I
EcoNI EcoT22I FseI FspI HincII
HindII HindIII HpaI KspI MaeI
MamI MfeI MluI MroI MseI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tru9I Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 65 enzymes) #
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Acc65I 886 GGTACC G^GTACC
AccI 645 GTCTAC GT^[AC][GT]AC
AccII 75 CGCG CG^CG
AflIII 692 ACATGT A^C[GA][CT]GT
Aor51HI 1035 AGCGCT AGC^GCT
Asp718I 886 GGTACC G^GTACC
AspHI 1416 GAGCAC G[AT]GC[AT]^C
AvaII 1284 GGTCC G^G[AT]CC
BalI 666 TGGCCA TGG^CCA
BbeI 97 GGCGCC GGCGC^C
BbrPI 614 CACGTG CAC^GTG
BciVI 1468 GGATAC GTATCC(6/5)
BglI 115 GCCAACACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1523 GCTCAGC GC^T[ACGT]AGC
BpmI 900 CTGGAG CTGGAG(16/14)
Bpu1102I 1523 GCTCAGC GC^T[ACGT]AGC
BsaI 1644 GGTCTC GGTCTC(1/5)
BsiEI 1211 CGGCCG CG[GA][CT]^CG
BsiHKAI 1416 GAGCAC G[AT]GC[AT]^C
BsmFI 851 GGGAC GGGAC(10/14)
BsmI 600 GAATGC GAATGC(1/-1)
Bsp1407I 1232 TGTACA T^GTACA
BspDI 1054 ATCGAT AT^CGAT
BspLU11I 692 ACATGT A^CATGT
BspMI 1711 ACCTGC ACCTGC(4/8)
BsrFI 329 ACCGGC [GA]^CCGG[CT]
BsrGI 1232 TGTACA T^GTACA
BssSI 1455 CACGAG CACGAG(-5/-1)
BstEII 1102 GGTGACC G^GT[ACGT]ACC
BstPI 1102 GGTGACC G^GT[ACGT]ACC
BstUI 75 CGCG CG^CG
BstXI 24 CCAACGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1523 GCTCAGC GC^T[ACGT]AGC
Cfr10I 329 ACCGGC [GA]^CCGG[CT]
ClaI 1054 ATCGAT AT^CGAT
DraII 1226 AGGCCCT [GA]G^G[ACGT]CC[CT]
EagI 1211 CGGCCG C^GGCCG
EclXI 1211 CGGCCG C^GGCCG
Eco47III 1035 AGCGCT AGC^GCT
Eco52I 1211 CGGCCG C^GGCCG
EcoO109I 1226 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1102 GGTGACC G^GT[ACGT]ACC
EcoRV 1746 GATATC GAT^ATC
EcoT14I 1568 CCATGG C^C[AT][AT]GG
HgaI 112 GACGC GACGC(5/10)
KasI 97 GGCGCC G^GCGCC
KpnI 886 GGTACC GGTAC^C
MluNI 666 TGGCCA TGG^CCA
MscI 666 TGGCCA TGG^CCA
MvnI 75 CGCG CG^CG
NarI 97 GGCGCC GG^CGCC
NcoI 1568 CCATGG C^CATGG
PmaCI 614 CACGTG CAC^GTG
PmlI 614 CACGTG CAC^GTG
PstI 1599 CTGCAG CTGCA^G
PvuII 1526 CAGCTG CAG^CTG
SapI 49 GAAGAGC GCTCTTC(1/4)
SfoI 97 GGCGCC GGC^GCC
SmaI 149 CCCGGG CCC^GGG
SmlI 1383 CTCAAG C^T[CT][GA]AG
SspBI 1232 TGTACA T^GTACA
StyI 1568 CCATGG C^C[AT][AT]GG
XcmI 1559 CCAGCCCCACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 149 CCCGGG C^CCGGG
XmaI 149 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcyI 97 GGCGCC G[GA]^CG[CT]C
112 GACGCC
AlwI 535 GATCC GGATC(4/5)
1149 GGATC
AlwNI 483 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
680 CAGTCTCTG
BanII 152 GGGCTC G[GA]GC[CT]^C
728 GGGCTC
BclI 839 TGATCA T^GATCA
911 TGATCA
BsaHI 97 GGCGCC G[GA]^CG[CT]C
112 GACGCC
BsrBI 132 GAGCGG CCGCTC(-3/-3)
1208 GAGCGG
BstYI 534 AGATCC [GA]^GATC[CT]
1149 GGATCT
DsaI 242 CCGTGG C^C[GA][CT]GG
1568 CCATGG
EcoRI 320 GAATTC G^AATTC
1308 GAATTC
FbaI 839 TGATCA T^GATCA
911 TGATCA
HaeII 97 GGCGCC [GA]GCGC^[CT]
1035 AGCGCT
Hin1I 97 GGCGCC G[GA]^CG[CT]C
112 GACGCC
MflI 534 AGATCC [GA]^GATC[CT]
1149 GGATCT
SfcI 345 CTACAG C^T[GA][CT]AG
1599 CTGCAG
TaqI 251 TCGA T^CGA
1055 TCGA
TthHB8I 251 TCGA T^CGA
1055 TCGA
XhoII 534 AGATCC [GA]^GATC[CT]
1149 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
Asp700I AvaI BanI BbvI BcnI
BmyI BsaAI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsoBI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
EaeI EarI EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI TfiI TseI Tsp45I
Tsp509I TspRI Van91I XmnI