(Circular) (Six-base) MAPSORT of: pFN21AB7804.seq Check: 9135 from: 1 to: 6478 pFN21AB7804 6478 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3725 3725 Size: 6478 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4288 278 Size: 53 83 186 936 2752 2468 Fragments arranged by size: 2752 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2651 3821 1937 Size: 714 1170 4594 Fragments arranged by size: 4594 1170 714 Acc65I G'GTAC_C Cuts at: 2891 3799 2891 Size: 908 5570 AclI AA'CG_TT Cuts at: 1563 4605 4978 1563 Size: 3042 373 3063 Fragments arranged by size: 3063 3042 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2324 3227 4541 5925 6193 1632 Size: 692 903 1314 1384 268 1917 Fragments arranged by size: 1917 1384 1314 903 692 268 AfeI AGC'GCT Cuts at: 3042 3042 Size: 6478 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6281 AflIII A'CryG_T Cuts at: 1203 2697 5377 6354 1203 Size: 1494 2680 977 1327 Fragments arranged by size: 2680 1494 1327 977 AgeI A'CCGG_T Cuts at: 5309 5309 Size: 6478 AhdI GACnn_n'nnGTC Cuts at: 5207 5207 Size: 6478 AleI CACnn'nnGTG Cuts at: 1166 3105 1166 Size: 1939 4539 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6478 AlwNI CAG_nnn'CTG Cuts at: 2493 2690 4221 5793 2493 Size: 197 1531 1572 3178 Fragments arranged by size: 3178 1572 1531 197 ApaLI G'TGCA_C Cuts at: 4535 5691 4535 Size: 1156 5322 ApoI r'AATT_y Cuts at: 1493 2325 2570 3313 3787 4013 4165 6274 1493 Size: 832 245 743 474 226 152 2109 1697 Fragments arranged by size: 2109 1697 832 743 474 245 226 152 AseI AT'TA_AT Cuts at: 160 1032 5032 160 Size: 872 4000 1606 Fragments arranged by size: 4000 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6478 AvaI C'yCGr_G Cuts at: 1870 1943 2154 2809 3616 3803 1870 Size: 73 211 655 807 187 4545 Fragments arranged by size: 4545 807 655 211 187 73 BamHI G'GATC_C Cuts at: 3808 4175 3808 Size: 367 6111 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2102 2891 3099 3799 5254 Size: 325 217 627 315 789 208 700 1455 Cuts at: 5254 618 Size: 1842 Fragments arranged by size: 1842 1455 789 700 627 325 315 217 208 BanII G_rGCy'C Cuts at: 1391 2161 2737 3797 1391 Size: 770 576 1060 4072 Fragments arranged by size: 4072 1060 770 576 BbeI G_GCGC'C Cuts at: 2106 2106 Size: 6478 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5507 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4669 4703 1903 Size: 34 2732 34 3678 Fragments arranged by size: 3678 2732 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3467 4370 5580 3467 Size: 903 1210 4365 Fragments arranged by size: 4365 1210 903 BclI T'GATC_A Cuts at: 1548 2844 2916 1548 Size: 1296 72 5110 Fragments arranged by size: 5110 1296 72 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2126 5089 136 Size: 107 122 71 631 605 454 2963 1525 Fragments arranged by size: 2963 1525 631 605 454 122 107 71 BglII A'GATC_T Cuts at: 6473 6473 Size: 6478 BlpI GC'TnA_GC Cuts at: 3529 3895 3529 Size: 366 6112 Bme1580I G_kGCm'C Cuts at: 1790 4539 5695 1790 Size: 2749 1156 2573 Fragments arranged by size: 2749 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2091 1318 Size: 773 5705 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4209 5167 442 Size: 416 53 83 399 24 2792 958 1753 Fragments arranged by size: 2792 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6478 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2483 2551 3444 2483 Size: 68 893 5517 Fragments arranged by size: 5517 893 68 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2926 5138 1239 Size: 1687 2212 2579 Fragments arranged by size: 2579 2212 1687 Bpu10I CC'TnA_GC Cuts at: 3114 3114 Size: 6478 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3373 4603 5468 5766 3373 Size: 1230 865 298 4085 Fragments arranged by size: 4085 1230 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3655 5141 882 Size: 617 2156 1486 2219 Fragments arranged by size: 2219 2156 1486 617 BsaAI yAC'GTr Cuts at: 493 1204 2386 2621 3415 4269 493 Size: 711 1182 235 794 854 2702 Fragments arranged by size: 2702 1182 854 794 711 235 BsaBI GATnn'nnATC Cuts at: 6472 6472 Size: 6478 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2103 2118 4285 4667 Size: 53 83 186 936 570 15 2167 382 Cuts at: 4667 5302 275 Size: 635 1451 Fragments arranged by size: 2167 1451 936 635 570 382 186 83 53 15 BsaWI w'CCGG_w Cuts at: 4911 5309 5583 5730 6141 4911 Size: 398 274 147 411 5248 Fragments arranged by size: 5248 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3434 3464 1178 Size: 30 165 30 2031 30 4192 Fragments arranged by size: 4192 2031 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2913 2940 3571 3604 1186 Size: 1727 27 631 33 4060 Fragments arranged by size: 4060 1727 631 33 27 BseYI C'CCAG_C Cuts at: 2062 3432 5681 6177 2062 Size: 1370 2249 496 2363 Fragments arranged by size: 2363 2249 1370 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2506 2960 3703 1163 Size: 573 770 454 743 3938 Fragments arranged by size: 3938 770 743 573 454 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3219 3928 4689 5717 1540 Size: 275 186 1218 709 761 1028 2301 Fragments arranged by size: 2301 1218 1028 761 709 275 186 BsiHKAI G_wGCw'C Cuts at: 3425 4539 4624 5695 3425 Size: 1114 85 1071 4208 Fragments arranged by size: 4208 1114 1071 85 BsmI GAATG_Cn' Cuts at: 2611 3989 4082 2611 Size: 1378 93 5007 Fragments arranged by size: 5007 1378 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2161 2737 3425 3797 4539 4624 5695 Size: 399 371 576 688 372 742 85 1071 Cuts at: 5695 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 688 576 399 372 371 85 BspEI T'CCGG_A Cuts at: 6141 6141 Size: 6478 BspHI T'CATG_A Cuts at: 4367 6097 4367 Size: 1730 4748 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3725 3833 844 Size: 2881 108 3489 Fragments arranged by size: 3489 2881 108 BsrBI CCG'CTC Cuts at: 1394 1823 2139 3215 4365 1394 Size: 429 316 1076 1150 3507 Fragments arranged by size: 3507 1150 1076 429 316 BsrDI GCAATG_nn' Cuts at: 65 1248 2362 3222 3495 4973 5147 65 Size: 1183 1114 860 273 1478 174 1396 Fragments arranged by size: 1478 1396 1183 1114 860 273 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2334 5122 5309 1700 Size: 110 524 2788 187 2869 Fragments arranged by size: 2869 2788 524 187 110 BsrGI T'GTAC_A Cuts at: 96 3237 96 Size: 3141 3337 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6478 BssSI C'ACGA_G Cuts at: 3460 4538 5550 3460 Size: 1078 1012 4388 Fragments arranged by size: 4388 1078 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6478 BstBI TT'CG_AA Cuts at: 5282 5282 Size: 6478 BstEII G'GTnAC_C Cuts at: 1184 3107 1184 Size: 1923 4555 BstXI CCAn_nnnn'nTGG Cuts at: 2036 2036 Size: 6478 BstYI r'GATC_y Cuts at: 1972 2539 3154 3808 4175 4560 4577 6029 6115 Size: 567 615 654 367 385 17 1452 86 Cuts at: 6115 6127 6473 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 654 615 567 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2247 3573 513 Size: 552 1182 1326 3418 Fragments arranged by size: 3418 1326 1182 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5739 Fragments arranged by size: 5739 683 56 BtsI GCAGTG_nn' Cuts at: 3994 4788 4808 3994 Size: 794 20 5664 Fragments arranged by size: 5664 794 20 ClaI AT'CG_AT Cuts at: 1554 3060 1554 Size: 1506 4972 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6443 DraI TTT'AAA Cuts at: 3782 3782 Size: 6478 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5485 809 Size: 507 4169 1802 Fragments arranged by size: 4169 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2671 3216 3333 Size: 54 1425 50 275 18 841 545 117 Cuts at: 3333 3925 4814 6443 8 Size: 592 889 1629 43 Fragments arranged by size: 1629 1425 889 841 592 545 275 117 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3216 3925 1537 Size: 275 1404 709 4090 Fragments arranged by size: 4090 1404 709 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2049 3203 3662 4408 1353 Size: 696 1154 459 746 3423 Fragments arranged by size: 3423 1154 746 696 459 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2743 5065 5439 5585 387 Size: 1064 138 1154 2322 374 146 1280 Fragments arranged by size: 2322 1280 1154 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2324 2926 3227 4541 5138 5925 6193 Size: 393 692 602 301 1314 597 787 268 Cuts at: 6193 1239 Size: 1524 Fragments arranged by size: 1524 1314 787 692 602 597 393 301 268 EcoO109I rG'GnC_Cy Cuts at: 3232 3232 Size: 6478 EcoRI G'AATT_C Cuts at: 2325 3313 3787 4165 2325 Size: 988 474 378 4638 Fragments arranged by size: 4638 988 474 378 EcoRV GAT'ATC Cuts at: 3753 3753 Size: 6478 FspI TGC'GCA Cuts at: 4984 4984 Size: 6478 HaeII r_GCGC'y Cuts at: 1678 2106 3044 5625 1678 Size: 428 938 2581 2531 Fragments arranged by size: 2581 2531 938 428 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2625 2657 2908 2935 2940 Size: 32 135 32 1053 32 251 27 5 Cuts at: 2940 2967 3754 3786 5119 5151 5193 5225 1373 Size: 27 787 32 1333 32 42 32 2626 Fragments arranged by size: 2626 1333 1053 787 251 135 42 32 32 32 32 32 32 27 27 5 HincII GTy'rAC Cuts at: 669 1657 1938 3822 4068 669 Size: 988 281 1884 246 3079 Fragments arranged by size: 3079 1884 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6478 HpaI GTT'AAC Cuts at: 4068 4068 Size: 6478 KasI G'GCGC_C Cuts at: 2102 2102 Size: 6478 KpnI G_GTAC'C Cuts at: 2895 3803 2895 Size: 908 5570 MfeI C'AATT_G Cuts at: 4077 4077 Size: 6478 MluI A'CGCG_T Cuts at: 6354 6354 Size: 6478 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2053 2223 3624 5592 5776 1305 Size: 748 170 1401 1968 184 2007 Fragments arranged by size: 2007 1968 1401 748 184 170 MscI TGG'CCA Cuts at: 10 64 1489 1832 2673 10 Size: 54 1425 343 841 3815 Fragments arranged by size: 3815 1425 841 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2936 3105 3464 4436 4795 4954 518 Size: 648 1770 169 359 972 359 159 2042 Fragments arranged by size: 2042 1770 972 648 359 359 169 159 MspA1I CmG'CkG Cuts at: 1606 2062 2070 2493 3533 3894 4571 5719 5964 Size: 456 8 423 1040 361 677 1148 245 Cuts at: 5964 1606 Size: 2120 Fragments arranged by size: 2120 1148 1040 677 456 423 361 245 8 NarI GG'CG_CC Cuts at: 2103 2103 Size: 6478 NcoI C'CATG_G Cuts at: 513 1065 3573 513 Size: 552 2508 3418 Fragments arranged by size: 3418 2508 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6478 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6478 NotI GC'GGCC_GC Cuts at: 3925 3925 Size: 6478 NspI r_CATG'y Cuts at: 1132 1229 2629 2701 2783 3836 5381 1132 Size: 97 1400 72 82 1053 1545 2229 Fragments arranged by size: 2229 1545 1400 1053 97 82 72 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6478 PciI A'CATG_T Cuts at: 2697 5377 2697 Size: 2680 3798 PflMI CCAn_nnn'nTGG Cuts at: 1108 2248 2644 2680 1108 Size: 1140 396 36 4906 Fragments arranged by size: 4906 1140 396 36 PmeI GTTT'AAAC Cuts at: 3782 3782 Size: 6478 PmlI CAC'GTG Cuts at: 2621 2621 Size: 6478 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4547 4579 6060 6092 4547 Size: 32 1481 32 4933 Fragments arranged by size: 4933 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6478 PsiI TTA'TAA Cuts at: 4048 4048 Size: 6478 PstI C_TGCA'G Cuts at: 830 3608 3830 830 Size: 2778 222 3478 Fragments arranged by size: 3478 2778 222 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6478 PvuII CAG'CTG Cuts at: 3533 3533 Size: 6478 SalI G'TCGA_C Cuts at: 1936 3820 1936 Size: 1884 4594 SapI GCTCTTCn'nnn_ Cuts at: 2049 2049 Size: 6478 SbfI CC_TGCA'GG Cuts at: 3830 3830 Size: 6478 ScaI AGT'ACT Cuts at: 4726 6438 4726 Size: 1712 4766 SfcI C'TryA_G Cuts at: 826 1045 2350 3604 3826 4961 5642 5833 826 Size: 219 1305 1254 222 1135 681 191 1471 Fragments arranged by size: 1471 1305 1254 1135 681 222 219 191 SfoI GGC'GCC Cuts at: 2104 2104 Size: 6478 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6478 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6478 SmaI CCC'GGG Cuts at: 1872 2156 3805 1872 Size: 284 1649 4545 Fragments arranged by size: 4545 1649 284 SmlI C'TyrA_G Cuts at: 820 1017 1943 3388 4582 5483 5745 820 Size: 197 926 1445 1194 901 262 1553 Fragments arranged by size: 1553 1445 1194 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4269 493 Size: 3776 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6478 SphI G_CATG'C Cuts at: 1132 3836 1132 Size: 2704 3774 SrfI GCCC'GGGC Cuts at: 2156 2156 Size: 6478 SspI AAT'ATT Cuts at: 5 52 4402 5 Size: 47 4350 2081 Fragments arranged by size: 4350 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6478 StyI C'CwwG_G Cuts at: 513 1065 3573 3917 513 Size: 552 2508 344 3074 Fragments arranged by size: 3074 2508 552 344 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3277 4505 4522 4675 4860 5305 325 Size: 1255 1697 1228 17 153 185 445 1498 Fragments arranged by size: 1697 1498 1255 1228 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3237 4724 6436 Size: 274 80 33 51 1443 1260 1487 1712 Cuts at: 6436 96 Size: 138 Fragments arranged by size: 1712 1487 1443 1260 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6438 6470 6438 Size: 32 6446 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6478 XbaI T'CTAG_A Cuts at: 3814 3814 Size: 6478 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3571 3571 Size: 6478 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6478 XmaI C'CCGG_G Cuts at: 1870 2154 3803 1870 Size: 284 1649 4545 Fragments arranged by size: 4545 1649 284 XmnI GAAnn'nnTTC Cuts at: 2573 3201 3317 4607 2573 Size: 628 116 1290 4444 Fragments arranged by size: 4444 1290 628 116 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4286 276 Size: 53 83 186 936 2752 2468 Fragments arranged by size: 2752 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SwaI