Restriction map for
pFN21AB7804
(Linear) (Six-base) MAPSORT of: KIBB7804.seq Check: 9410 from: 1 to: 1773
KIBB7804.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1720 1773
Size: 1720 53
AccI GT'mk_AC
Cuts at: 0 646 1773
Size: 646 1127
Acc65I G'GTAC_C
Cuts at: 0 886 1773
Size: 886 887
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 1222 1773
Size: 319 903 551
Fragments arranged by size:
903 551 319
AfeI AGC'GCT
Cuts at: 0 1037 1773
Size: 1037 736
AflIII A'CryG_T
Cuts at: 0 692 1773
Size: 692 1081
AleI CACnn'nnGTG
Cuts at: 0 1100 1773
Size: 1100 673
AlwNI CAG_nnn'CTG
Cuts at: 0 488 685 1773
Size: 488 197 1088
Fragments arranged by size:
1088 488 197
ApoI r'AATT_y
Cuts at: 0 320 565 1308 1773
Size: 320 245 743 465
Fragments arranged by size:
743 465 320 245
AvaI C'yCGr_G
Cuts at: 0 149 804 1611 1773
Size: 149 655 807 162
Fragments arranged by size:
807 655 162 149
BanI G'GyrC_C
Cuts at: 0 97 886 1094 1773
Size: 97 789 208 679
Fragments arranged by size:
789 679 208 97
BanII G_rGCy'C
Cuts at: 0 156 732 1773
Size: 156 576 1041
Fragments arranged by size:
1041 576 156
BbeI G_GCGC'C
Cuts at: 0 101 1773
Size: 101 1672
BciVI GTATCCnnnnn_n'
Cuts at: 0 1462 1773
Size: 1462 311
BclI T'GATC_A
Cuts at: 0 839 911 1773
Size: 839 72 862
Fragments arranged by size:
862 839 72
BglI GCCn_nnn'nGGC
Cuts at: 0 121 1773
Size: 121 1652
BlpI GC'TnA_GC
Cuts at: 0 1524 1773
Size: 1524 249
BmgBI CAC'GTC
Cuts at: 0 86 1773
Size: 86 1687
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 478 546 1439 1773
Size: 478 68 893 334
Fragments arranged by size:
893 478 334 68
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 921 1773
Size: 921 852
Bpu10I CC'TnA_GC
Cuts at: 0 1109 1773
Size: 1109 664
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1368 1773
Size: 1368 405
BsaI GGTCTCn'nnnn_
Cuts at: 0 1650 1773
Size: 1650 123
BsaAI yAC'GTr
Cuts at: 0 381 616 1410 1773
Size: 381 235 794 363
Fragments arranged by size:
794 381 363 235
BsaHI Gr'CG_yC
Cuts at: 0 98 113 1773
Size: 98 15 1660
Fragments arranged by size:
1660 98 15
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1429 1459 1773
Size: 1429 30 314
Fragments arranged by size:
1429 314 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 908 935 1566 1599 1773
Size: 908 27 631 33 174
Fragments arranged by size:
908 631 174 33 27
BseYI C'CCAG_C
Cuts at: 0 57 1427 1773
Size: 57 1370 346
Fragments arranged by size:
1370 346 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 501 955 1698 1773
Size: 501 454 743 75
Fragments arranged by size:
743 501 454 75
BsiEI CG_ry'CG
Cuts at: 0 1214 1773
Size: 1214 559
BsiHKAI G_wGCw'C
Cuts at: 0 1420 1773
Size: 1420 353
BsmI GAATG_Cn'
Cuts at: 0 606 1773
Size: 606 1167
Bsp1286I G_dGCh'C
Cuts at: 0 156 732 1420 1773
Size: 156 576 688 353
Fragments arranged by size:
688 576 353 156
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1720 1773
Size: 1720 53
BsrBI CCG'CTC
Cuts at: 0 134 1210 1773
Size: 134 1076 563
Fragments arranged by size:
1076 563 134
BsrDI GCAATG_nn'
Cuts at: 0 357 1217 1490 1773
Size: 357 860 273 283
Fragments arranged by size:
860 357 283 273
BsrFI r'CCGG_y
Cuts at: 0 329 1773
Size: 329 1444
BsrGI T'GTAC_A
Cuts at: 0 1232 1773
Size: 1232 541
BssSI C'ACGA_G
Cuts at: 0 1455 1773
Size: 1455 318
BstEII G'GTnAC_C
Cuts at: 0 1102 1773
Size: 1102 671
BstXI CCAn_nnnn'nTGG
Cuts at: 0 31 1773
Size: 31 1742
BstYI r'GATC_y
Cuts at: 0 534 1149 1773
Size: 534 615 624
Fragments arranged by size:
624 615 534
BtgI C'CryG_G
Cuts at: 0 242 1568 1773
Size: 242 1326 205
Fragments arranged by size:
1326 242 205
ClaI AT'CG_AT
Cuts at: 0 1055 1773
Size: 1055 718
EaeI y'GGCC_r
Cuts at: 0 666 1211 1328 1773
Size: 666 545 117 445
Fragments arranged by size:
666 545 445 117
EagI C'GGCC_G
Cuts at: 0 1211 1773
Size: 1211 562
EarI CTCTTCn'nnn_
Cuts at: 0 44 1198 1657 1773
Size: 44 1154 459 116
Fragments arranged by size:
1154 459 116 44
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 738 1773
Size: 738 1035
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 921 1222 1773
Size: 319 602 301 551
Fragments arranged by size:
602 551 319 301
EcoO109I rG'GnC_Cy
Cuts at: 0 1227 1773
Size: 1227 546
EcoRI G'AATT_C
Cuts at: 0 320 1308 1773
Size: 320 988 465
Fragments arranged by size:
988 465 320
EcoRV GAT'ATC
Cuts at: 0 1748 1773
Size: 1748 25
HaeII r_GCGC'y
Cuts at: 0 101 1039 1773
Size: 101 938 734
Fragments arranged by size:
938 734 101
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 620 652 903 930 935 962 1749 1773
Size: 620 32 251 27 5 27 787 24
Fragments arranged by size:
787 620 251 32 27 27 24 5
KasI G'GCGC_C
Cuts at: 0 97 1773
Size: 97 1676
KpnI G_GTAC'C
Cuts at: 0 890 1773
Size: 890 883
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 218 1619 1773
Size: 48 170 1401 154
Fragments arranged by size:
1401 170 154 48
MscI TGG'CCA
Cuts at: 0 668 1773
Size: 668 1105
MslI CAynn'nnrTG
Cuts at: 0 931 1100 1459 1773
Size: 931 169 359 314
Fragments arranged by size:
931 359 314 169
MspA1I CmG'CkG
Cuts at: 0 57 65 488 1528 1773
Size: 57 8 423 1040 245
Fragments arranged by size:
1040 423 245 57 8
NarI GG'CG_CC
Cuts at: 0 98 1773
Size: 98 1675
NcoI C'CATG_G
Cuts at: 0 1568 1773
Size: 1568 205
NspI r_CATG'y
Cuts at: 0 624 696 778 1773
Size: 624 72 82 995
Fragments arranged by size:
995 624 82 72
PciI A'CATG_T
Cuts at: 0 692 1773
Size: 692 1081
PflMI CCAn_nnn'nTGG
Cuts at: 0 243 639 675 1773
Size: 243 396 36 1098
Fragments arranged by size:
1098 396 243 36
PmlI CAC'GTG
Cuts at: 0 616 1773
Size: 616 1157
PstI C_TGCA'G
Cuts at: 0 1603 1773
Size: 1603 170
PvuII CAG'CTG
Cuts at: 0 1528 1773
Size: 1528 245
SapI GCTCTTCn'nnn_
Cuts at: 0 44 1773
Size: 44 1729
SfcI C'TryA_G
Cuts at: 0 345 1599 1773
Size: 345 1254 174
Fragments arranged by size:
1254 345 174
SfoI GGC'GCC
Cuts at: 0 99 1773
Size: 99 1674
SmaI CCC'GGG
Cuts at: 0 151 1773
Size: 151 1622
SmlI C'TyrA_G
Cuts at: 0 1383 1773
Size: 1383 390
SrfI GCCC'GGGC
Cuts at: 0 151 1773
Size: 151 1622
StyI C'CwwG_G
Cuts at: 0 1568 1773
Size: 1568 205
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1272 1773
Size: 1272 501
TatI w'GTAC_w
Cuts at: 0 1232 1773
Size: 1232 541
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1566 1773
Size: 1566 207
XmaI C'CCGG_G
Cuts at: 0 149 1773
Size: 149 1624
XmnI GAAnn'nnTTC
Cuts at: 0 568 1196 1312 1773
Size: 568 628 116 461
Fragments arranged by size:
628 568 461 116
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AleI AlwNI
ApoI AvaI BanI BanII BbeI BciVI BclI BglI
BlpI BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstEII
BstXI BstYI BtgI ClaI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI EcoRV HaeII Hin4I KasI KpnI
MmeI MscI MslI MspA1I NarI NcoI NspI PciI
PflMI PmlI PstI PvuII SapI SfcI SfoI SmaI
SmlI SrfI StyI TaqII TatI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AflII AgeI AhdI AlfI AloI ApaI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbsI
BbvCI BcgI BfrBI BglII Bme1580I BmrI BmtI BsaBI
BsaWI BsiWI BsmBI BspEI BspHI BssHII BstAPI BstBI
BstZ17I Bsu36I BtgZI BtsI CspCI DraI DraIII DrdI
EcoICRI EcoNI FalI FseI FspI FspAI HincII HindIII
HpaI MfeI MluI NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI
SgrAI SnaBI SpeI SphI SspI StuI SwaI TstI
Tth111I XbaI XhoI ZraI