(Circular) (Six-base) MAPSORT of: pFN21AB9397.seq Check: 7232 from: 1 to: 6655 pFN21AB9397 6655 bp With 182 enzymes: SgfI * October 20, 2009 10:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2824 2824 Size: 6655 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4465 278 Size: 53 83 186 936 2929 2468 Fragments arranged by size: 2929 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3998 1937 Size: 2061 4594 Acc65I G'GTAC_C Cuts at: 3976 3976 Size: 6655 AclI AA'CG_TT Cuts at: 1563 4782 5155 1563 Size: 3219 373 3063 Fragments arranged by size: 3219 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2208 2232 2296 2387 4718 6102 6370 1632 Size: 576 24 64 91 2331 1384 268 1917 Fragments arranged by size: 2331 1917 1384 576 268 91 64 24 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6458 AflIII A'CryG_T Cuts at: 1203 3168 5554 6531 1203 Size: 1965 2386 977 1327 Fragments arranged by size: 2386 1965 1327 977 AgeI A'CCGG_T Cuts at: 5486 5486 Size: 6655 AhdI GACnn_n'nnGTC Cuts at: 5384 5384 Size: 6655 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6655 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6655 AlwNI CAG_nnn'CTG Cuts at: 2195 4398 5970 2195 Size: 2203 1572 2880 Fragments arranged by size: 2880 2203 1572 ApaLI G'TGCA_C Cuts at: 4712 5868 4712 Size: 1156 5499 ApoI r'AATT_y Cuts at: 1493 2906 3223 3964 4190 4342 6451 1493 Size: 1413 317 741 226 152 2109 1697 Fragments arranged by size: 2109 1697 1413 741 317 226 152 AseI AT'TA_AT Cuts at: 160 1032 5209 160 Size: 872 4177 1606 Fragments arranged by size: 4177 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6655 AvaI C'yCGr_G Cuts at: 1870 1943 2826 3111 3980 1870 Size: 73 883 285 869 4545 Fragments arranged by size: 4545 883 869 285 73 AvrII C'CTAG_G Cuts at: 3863 3863 Size: 6655 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2399 2432 2399 Size: 33 6622 BamHI G'GATC_C Cuts at: 3735 3985 4352 3735 Size: 250 367 6038 Fragments arranged by size: 6038 367 250 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2555 3976 5431 618 Size: 325 217 627 768 1421 1455 1842 Fragments arranged by size: 1842 1455 1421 768 627 325 217 BanII G_rGCy'C Cuts at: 1391 2772 3974 1391 Size: 1381 1202 4072 Fragments arranged by size: 4072 1381 1202 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2361 2447 2463 928 Size: 971 462 86 16 5120 Fragments arranged by size: 5120 971 462 86 16 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4846 4880 1903 Size: 34 2909 34 3678 Fragments arranged by size: 3678 2909 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2702 4547 5757 2702 Size: 1845 1210 3600 Fragments arranged by size: 3600 1845 1210 BclI T'GATC_A Cuts at: 1548 2592 1548 Size: 1044 5611 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2275 5266 136 Size: 107 122 71 631 605 603 2991 1525 Fragments arranged by size: 2991 1525 631 605 603 122 107 71 BglII A'GATC_T Cuts at: 2807 3459 6650 2807 Size: 652 3191 2812 Fragments arranged by size: 3191 2812 652 BlpI GC'TnA_GC Cuts at: 3714 4072 3714 Size: 358 6297 Bme1580I G_kGCm'C Cuts at: 1790 2560 4716 5872 1790 Size: 770 2156 1156 2573 Fragments arranged by size: 2573 2156 1156 770 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6655 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3741 4386 5344 Size: 416 53 83 399 24 2324 645 958 Cuts at: 5344 442 Size: 1753 Fragments arranged by size: 2324 1753 958 645 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6655 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2979 3603 2979 Size: 624 6031 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3776 5315 1239 Size: 2537 1539 2579 Fragments arranged by size: 2579 2537 1539 Bpu10I CC'TnA_GC Cuts at: 3693 3693 Size: 6655 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2974 3581 3909 4780 5645 5943 2974 Size: 607 328 871 865 298 3686 Fragments arranged by size: 3686 871 865 607 328 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2174 5318 882 Size: 617 675 3144 2219 Fragments arranged by size: 3144 2219 675 617 BsaAI yAC'GTr Cuts at: 493 1204 4446 493 Size: 711 3242 2702 Fragments arranged by size: 3242 2702 711 BsaBI GATnn'nnATC Cuts at: 3048 6649 3048 Size: 3601 3054 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 2942 4462 4844 Size: 53 83 186 936 476 933 1520 382 Cuts at: 4844 5479 275 Size: 635 1451 Fragments arranged by size: 1520 1451 936 933 635 476 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3738 5088 5486 5760 5907 6318 3738 Size: 1350 398 274 147 411 4075 Fragments arranged by size: 4075 1350 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3899 3929 1178 Size: 30 165 30 2496 30 3904 Fragments arranged by size: 3904 2496 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6655 BseYI C'CCAG_C Cuts at: 5858 6354 5858 Size: 496 6159 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2802 1163 Size: 573 1066 5016 Fragments arranged by size: 5016 1066 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4105 4866 5894 1540 Size: 275 186 2104 761 1028 2301 Fragments arranged by size: 2301 2104 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3857 4716 4801 5872 3857 Size: 859 85 1071 4640 Fragments arranged by size: 4640 1071 859 85 BsmI GAATG_Cn' Cuts at: 2154 4166 4259 2154 Size: 2012 93 4550 Fragments arranged by size: 4550 2012 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3864 3864 Size: 6655 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2560 2772 3857 3974 4716 4801 5872 Size: 399 770 212 1085 117 742 85 1071 Cuts at: 5872 1391 Size: 2174 Fragments arranged by size: 2174 1085 1071 770 742 399 212 117 85 BspEI T'CCGG_A Cuts at: 3738 6318 3738 Size: 2580 4075 BspHI T'CATG_A Cuts at: 4544 6274 4544 Size: 1730 4925 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2203 2824 4010 844 Size: 1359 621 1186 3489 Fragments arranged by size: 3489 1359 1186 621 BsrBI CCG'CTC Cuts at: 1394 1823 3766 4542 1394 Size: 429 1943 776 3507 Fragments arranged by size: 3507 1943 776 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2087 3459 5150 5324 65 Size: 1183 839 1372 1691 174 1396 Fragments arranged by size: 1691 1396 1372 1183 839 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5299 5486 1700 Size: 110 3489 187 2869 Fragments arranged by size: 3489 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6655 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6655 BssSI C'ACGA_G Cuts at: 3590 4715 5727 3590 Size: 1125 1012 4518 Fragments arranged by size: 4518 1125 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3685 794 Size: 2891 3764 BstBI TT'CG_AA Cuts at: 5459 5459 Size: 6655 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6655 BstYI r'GATC_y Cuts at: 1972 2807 3244 3459 3735 3985 4352 4737 4754 Size: 835 437 215 276 250 367 385 17 Cuts at: 4754 6206 6292 6304 6650 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 835 437 385 367 346 276 250 215 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3830 513 Size: 552 939 1826 3338 Fragments arranged by size: 3338 1826 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2892 487 Size: 683 56 1666 4250 Fragments arranged by size: 4250 1666 683 56 BtsI GCAGTG_nn' Cuts at: 3310 4171 4965 4985 3310 Size: 861 794 20 4980 Fragments arranged by size: 4980 861 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6655 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6620 DraI TTT'AAA Cuts at: 3959 3959 Size: 6655 DraIII CAC_nnn'GTG Cuts at: 3285 3285 Size: 6655 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2465 5662 809 Size: 507 1149 3197 1802 Fragments arranged by size: 3197 1802 1149 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2723 3777 4102 Size: 54 1425 50 275 18 893 1054 325 Cuts at: 4102 4991 6620 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1054 893 889 325 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4102 1537 Size: 275 2290 4090 Fragments arranged by size: 4090 2290 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2245 3521 3639 4585 1353 Size: 892 1276 118 946 3423 Fragments arranged by size: 3423 1276 946 892 118 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2054 5242 5616 5762 387 Size: 1064 138 465 3188 374 146 1280 Fragments arranged by size: 3188 1280 1064 465 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2208 2232 2296 2387 3776 4718 5315 Size: 393 576 24 64 91 1389 942 597 Cuts at: 5315 6102 6370 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1389 942 787 597 576 393 268 91 64 24 EcoNI CCTnn'n_nnAGG Cuts at: 3862 3862 Size: 6655 EcoO109I rG'GnC_Cy Cuts at: 2192 2192 Size: 6655 EcoRI G'AATT_C Cuts at: 3964 4342 3964 Size: 378 6277 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2826 3950 2826 Size: 1124 5531 FspI TGC'GCA Cuts at: 5161 5161 Size: 6655 HaeII r_GCGC'y Cuts at: 1678 5802 1678 Size: 4124 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5296 5328 5370 5402 1373 Size: 32 135 32 3724 32 42 32 2626 Fragments arranged by size: 3724 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3162 3999 4245 669 Size: 988 281 1224 837 246 3079 Fragments arranged by size: 3079 1224 988 837 281 246 HindIII A'AGCT_T Cuts at: 748 3122 748 Size: 2374 4281 HpaI GTT'AAC Cuts at: 3162 4245 3162 Size: 1083 5572 KpnI G_GTAC'C Cuts at: 3980 3980 Size: 6655 MfeI C'AATT_G Cuts at: 4254 4254 Size: 6655 MluI A'CGCG_T Cuts at: 6531 6531 Size: 6655 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2037 2350 2817 3285 5769 5953 1305 Size: 732 313 467 468 2484 184 2007 Fragments arranged by size: 2484 2007 732 468 467 313 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2725 3779 10 Size: 54 1425 343 893 1054 2886 Fragments arranged by size: 2886 1425 1054 893 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2553 3147 4613 4972 5131 518 Size: 648 1387 594 1466 359 159 2042 Fragments arranged by size: 2042 1466 1387 648 594 359 159 MspA1I CmG'CkG Cuts at: 1606 2617 4071 4748 5896 6141 1606 Size: 1011 1454 677 1148 245 2120 Fragments arranged by size: 2120 1454 1148 1011 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3830 513 Size: 552 939 1826 3338 Fragments arranged by size: 3338 1826 939 552 NdeI CA'TA_TG Cuts at: 387 2862 387 Size: 2475 4180 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6655 NotI GC'GGCC_GC Cuts at: 4102 4102 Size: 6655 NspI r_CATG'y Cuts at: 1132 1229 2055 4013 5558 1132 Size: 97 826 1958 1545 2229 Fragments arranged by size: 2229 1958 1545 826 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6655 PciI A'CATG_T Cuts at: 5554 5554 Size: 6655 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6655 PmeI GTTT'AAAC Cuts at: 3959 3959 Size: 6655 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4724 4756 6237 6269 4724 Size: 32 1481 32 5110 Fragments arranged by size: 5110 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2192 2192 Size: 6655 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6655 PsiI TTA'TAA Cuts at: 3230 4225 3230 Size: 995 5660 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2391 2423 2391 Size: 32 6623 PstI C_TGCA'G Cuts at: 830 2565 4007 830 Size: 1735 1442 3478 Fragments arranged by size: 3478 1735 1442 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6655 SalI G'TCGA_C Cuts at: 1936 3997 1936 Size: 2061 4594 SbfI CC_TGCA'GG Cuts at: 2565 4007 2565 Size: 1442 5213 ScaI AGT'ACT Cuts at: 2650 4903 6615 2650 Size: 2253 1712 2690 Fragments arranged by size: 2690 2253 1712 SfcI C'TryA_G Cuts at: 826 1045 2561 2736 2901 4003 5138 5819 6010 Size: 219 1516 175 165 1102 1135 681 191 Cuts at: 6010 826 Size: 1471 Fragments arranged by size: 1516 1471 1135 1102 681 219 191 175 165 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6655 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6655 SmaI CCC'GGG Cuts at: 1872 2828 3982 1872 Size: 956 1154 4545 Fragments arranged by size: 4545 1154 956 SmlI C'TyrA_G Cuts at: 820 1017 1943 2953 3560 3924 4759 5660 5922 Size: 197 926 1010 607 364 835 901 262 Cuts at: 5922 820 Size: 1553 Fragments arranged by size: 1553 1010 926 901 835 607 364 262 197 SnaBI TAC'GTA Cuts at: 493 4446 493 Size: 3953 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6655 SphI G_CATG'C Cuts at: 1132 4013 1132 Size: 2881 3774 SspI AAT'ATT Cuts at: 5 52 4579 5 Size: 47 4527 2081 Fragments arranged by size: 4527 2081 47 StuI AGG'CCT Cuts at: 1517 3095 3545 1517 Size: 1578 450 4627 Fragments arranged by size: 4627 1578 450 StyI C'CwwG_G Cuts at: 513 1065 2004 3830 3863 4094 513 Size: 552 939 1826 33 231 3074 Fragments arranged by size: 3074 1826 939 552 231 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2615 4682 4699 4852 5037 5482 325 Size: 1255 1035 2067 17 153 185 445 1498 Fragments arranged by size: 2067 1498 1255 1035 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2054 2363 2622 Size: 274 80 33 51 1443 77 309 259 Cuts at: 2622 2648 3840 4901 6613 96 Size: 26 1192 1061 1712 138 Fragments arranged by size: 1712 1443 1192 1061 309 274 259 138 80 77 51 33 26 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3733 3765 6615 6647 3733 Size: 32 2850 32 3741 Fragments arranged by size: 3741 2850 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6655 XbaI T'CTAG_A Cuts at: 2389 3991 2389 Size: 1602 5053 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6655 XmaI C'CCGG_G Cuts at: 1870 2826 3980 1870 Size: 956 1154 4545 Fragments arranged by size: 4545 1154 956 XmnI GAAnn'nnTTC Cuts at: 2809 3612 4784 2809 Size: 803 1172 4680 Fragments arranged by size: 4680 1172 803 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4463 276 Size: 53 83 186 936 2929 2468 Fragments arranged by size: 2929 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BbeI BbvCI BfrBI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI