Restriction map for
pFN21AB9397
(Linear) (Six-base) MAPSORT of: KIBB9397.seq Check: 4327 from: 1 to: 1950
KIBB9397.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 819 1950
Size: 819 1131
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 227 291 382 1950
Size: 203 24 64 91 1568
Fragments arranged by size:
1568 203 91 64 24
AflIII A'CryG_T
Cuts at: 0 1163 1950
Size: 1163 787
AlwNI CAG_nnn'CTG
Cuts at: 0 190 1950
Size: 190 1760
ApoI r'AATT_y
Cuts at: 0 901 1218 1950
Size: 901 317 732
Fragments arranged by size:
901 732 317
AvaI C'yCGr_G
Cuts at: 0 821 1106 1950
Size: 821 285 844
Fragments arranged by size:
844 821 285
AvrII C'CTAG_G
Cuts at: 0 1858 1950
Size: 1858 92
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 394 427 1950
Size: 394 33 1523
Fragments arranged by size:
1523 394 33
BamHI G'GATC_C
Cuts at: 0 1730 1950
Size: 1730 220
BanI G'GyrC_C
Cuts at: 0 550 1950
Size: 550 1400
BanII G_rGCy'C
Cuts at: 0 767 1950
Size: 767 1183
BbsI GAAGACnn'nnnn_
Cuts at: 0 356 442 458 1950
Size: 356 86 16 1492
Fragments arranged by size:
1492 356 86 16
BciVI GTATCCnnnnn_n'
Cuts at: 0 697 1950
Size: 697 1253
BclI T'GATC_A
Cuts at: 0 587 1950
Size: 587 1363
BglI GCCn_nnn'nGGC
Cuts at: 0 270 1950
Size: 270 1680
BglII A'GATC_T
Cuts at: 0 802 1454 1950
Size: 802 652 496
Fragments arranged by size:
802 652 496
BlpI GC'TnA_GC
Cuts at: 0 1709 1950
Size: 1709 241
Bme1580I G_kGCm'C
Cuts at: 0 555 1950
Size: 555 1395
BmrI ACTGGGnnnn_n'
Cuts at: 0 1736 1950
Size: 1736 214
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 974 1598 1950
Size: 974 624 352
Fragments arranged by size:
974 624 352
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1771 1950
Size: 1771 179
Bpu10I CC'TnA_GC
Cuts at: 0 1688 1950
Size: 1688 262
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 969 1576 1904 1950
Size: 969 607 328 46
Fragments arranged by size:
969 607 328 46
BsaI GGTCTCn'nnnn_
Cuts at: 0 169 1950
Size: 169 1781
BsaBI GATnn'nnATC
Cuts at: 0 1043 1950
Size: 1043 907
BsaHI Gr'CG_yC
Cuts at: 0 4 937 1950
Size: 4 933 1013
Fragments arranged by size:
1013 933 4
BsaWI w'CCGG_w
Cuts at: 0 1733 1950
Size: 1733 217
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1894 1924 1950
Size: 1894 30 26
Fragments arranged by size:
1894 30 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 797 1950
Size: 797 1153
BsiHKAI G_wGCw'C
Cuts at: 0 1852 1950
Size: 1852 98
BsmI GAATG_Cn'
Cuts at: 0 149 1950
Size: 149 1801
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1859 1950
Size: 1859 91
Bsp1286I G_dGCh'C
Cuts at: 0 555 767 1852 1950
Size: 555 212 1085 98
Fragments arranged by size:
1085 555 212 98
BspEI T'CCGG_A
Cuts at: 0 1733 1950
Size: 1733 217
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 198 819 1950
Size: 198 621 1131
Fragments arranged by size:
1131 621 198
BsrBI CCG'CTC
Cuts at: 0 1761 1950
Size: 1761 189
BsrDI GCAATG_nn'
Cuts at: 0 82 1454 1950
Size: 82 1372 496
Fragments arranged by size:
1372 496 82
BssSI C'ACGA_G
Cuts at: 0 1585 1950
Size: 1585 365
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1680 1950
Size: 1680 270
BstYI r'GATC_y
Cuts at: 0 802 1239 1454 1730 1950
Size: 802 437 215 276 220
Fragments arranged by size:
802 437 276 220 215
BtgI C'CryG_G
Cuts at: 0 1825 1950
Size: 1825 125
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 887 1950
Size: 887 1063
BtsI GCAGTG_nn'
Cuts at: 0 1305 1950
Size: 1305 645
DraIII CAC_nnn'GTG
Cuts at: 0 1280 1950
Size: 1280 670
DrdI GACnn_nn'nnGTC
Cuts at: 0 460 1950
Size: 460 1490
EaeI y'GGCC_r
Cuts at: 0 718 1772 1950
Size: 718 1054 178
Fragments arranged by size:
1054 718 178
EarI CTCTTCn'nnn_
Cuts at: 0 240 1516 1634 1950
Size: 240 1276 118 316
Fragments arranged by size:
1276 316 240 118
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 49 1950
Size: 49 1901
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 227 291 382 1771 1950
Size: 203 24 64 91 1389 179
Fragments arranged by size:
1389 203 179 91 64 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1857 1950
Size: 1857 93
EcoO109I rG'GnC_Cy
Cuts at: 0 187 1950
Size: 187 1763
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 821 1945 1950
Size: 821 1124 5
Fragments arranged by size:
1124 821 5
HincII GTy'rAC
Cuts at: 0 1157 1950
Size: 1157 793
HindIII A'AGCT_T
Cuts at: 0 1117 1950
Size: 1117 833
HpaI GTT'AAC
Cuts at: 0 1157 1950
Size: 1157 793
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 345 812 1280 1950
Size: 32 313 467 468 670
Fragments arranged by size:
670 468 467 313 32
MscI TGG'CCA
Cuts at: 0 720 1774 1950
Size: 720 1054 176
Fragments arranged by size:
1054 720 176
MslI CAynn'nnrTG
Cuts at: 0 548 1142 1950
Size: 548 594 808
Fragments arranged by size:
808 594 548
MspA1I CmG'CkG
Cuts at: 0 612 1950
Size: 612 1338
NcoI C'CATG_G
Cuts at: 0 1825 1950
Size: 1825 125
NdeI CA'TA_TG
Cuts at: 0 857 1950
Size: 857 1093
NspI r_CATG'y
Cuts at: 0 50 1950
Size: 50 1900
PpuMI rG'GwC_Cy
Cuts at: 0 187 1950
Size: 187 1763
PsiI TTA'TAA
Cuts at: 0 1225 1950
Size: 1225 725
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 386 418 1950
Size: 386 32 1532
Fragments arranged by size:
1532 386 32
PstI C_TGCA'G
Cuts at: 0 560 1950
Size: 560 1390
SbfI CC_TGCA'GG
Cuts at: 0 560 1950
Size: 560 1390
ScaI AGT'ACT
Cuts at: 0 645 1950
Size: 645 1305
SfcI C'TryA_G
Cuts at: 0 556 731 896 1950
Size: 556 175 165 1054
Fragments arranged by size:
1054 556 175 165
SmaI CCC'GGG
Cuts at: 0 823 1950
Size: 823 1127
SmlI C'TyrA_G
Cuts at: 0 948 1555 1919 1950
Size: 948 607 364 31
Fragments arranged by size:
948 607 364 31
StuI AGG'CCT
Cuts at: 0 1090 1540 1950
Size: 1090 450 410
Fragments arranged by size:
1090 450 410
StyI C'CwwG_G
Cuts at: 0 1825 1858 1950
Size: 1825 33 92
Fragments arranged by size:
1825 92 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 610 1950
Size: 610 1340
TatI w'GTAC_w
Cuts at: 0 49 358 617 643 1835 1950
Size: 49 309 259 26 1192 115
Fragments arranged by size:
1192 309 259 115 49 26
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1728 1760 1950
Size: 1728 32 190
Fragments arranged by size:
1728 190 32
XbaI T'CTAG_A
Cuts at: 0 384 1950
Size: 384 1566
XmaI C'CCGG_G
Cuts at: 0 821 1950
Size: 821 1129
XmnI GAAnn'nnTTC
Cuts at: 0 804 1607 1950
Size: 804 803 343
Fragments arranged by size:
804 803 343
Enzymes that do cut:
AarI AcuI AflIII AlwNI ApoI AvaI AvrII BaeI
BamHI BanI BanII BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaWI BsaXI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrDI BssSI BstAPI BstYI
BtgI BtgZI BtsI DraIII DrdI EaeI EarI EciI
Eco57MI EcoNI EcoO109I FalI HincII HindIII HpaI MmeI
MscI MslI MspA1I NcoI NdeI NspI PpuMI PsiI
PsrI PstI SbfI ScaI SfcI SmaI SmlI StuI
StyI TaqII TatI TstI XbaI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
BbeI BbvCI BcgI BfrBI BmgBI BmtI BsaAI BseRI
BseYI BsiEI BsiWI BspHI BsrFI BsrGI BssHII BstBI
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraI EagI
EcoICRI EcoRI EcoRV FseI FspI FspAI HaeII Hin4I
KasI KpnI MfeI MluI NaeI NarI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PspOMI PspXI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI Tth111I XcmI XhoI ZraI