Restriction map for
pFN21AB9359
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# NO CUT ENZYMES ( 143 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlpI Bpu1102I BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstYI BstZ17I CelII
ClaI CpoI DraI DraIII EagI
Eam1105I EarI EclXI Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI Hin1I HincII HindII
HindIII HinfI HpaI HphI KasI
KpnI Ksp632I MamI MfeI MflI
MluI MroI MseI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SpeI
Sse8387I SspBI SspI SwaI TfiI
Tru9I Tsp509I Tth111I Van91I XbaI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AocI 762 CCTCAGG CC^T[ACGT]AGG
ApaI 829 GGGCCC GGGCC^C
AspHI 670 GAGCTC G[AT]GC[AT]^C
AvrII 432 CCTAGG C^CTAGG
BalI 239 TGGCCA TGG^CCA
BbsI 229 GTCTTC GAAGAC(2/6)
BfaI 433 CTAG C^TAG
BlnI 432 CCTAGG C^CTAGG
BpuAI 229 GTCTTC GAAGAC(2/6)
BsaAI 112 TACGTA [CT]AC^GT[GA]
BsaI 343 GAGACC GGTCTC(1/5)
BsiHKAI 670 GAGCTC G[AT]GC[AT]^C
BsrBI 773 CCGCTC CCGCTC(-3/-3)
BsrFI 497 ACCGGC [GA]^CCGG[CT]
BstAPI 84 GCAGAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 762 CCTCAGG CC^T[ACGT]AGG
Cfr10I 497 ACCGGC [GA]^CCGG[CT]
EaeI 239 TGGCCA [CT]^GGCC[GA]
Eco81I 762 CCTCAGG CC^T[ACGT]AGG
HgaI 272 GCGTC GACGC(5/10)
KspI 55 CCGCGG CCGC^GG
MaeI 433 CTAG C^TAG
MaeII 113 ACGT A^CGT
MluNI 239 TGGCCA TGG^CCA
MscI 239 TGGCCA TGG^CCA
MslI 702 CACTGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PpuMI 449 GGGACCT [GA]G^G[AT]CC[CT]
SacI 670 GAGCTC GAGCT^C
SacII 55 CCGCGG CCGC^GG
SmlI 442 CTCAAG C^T[CT][GA]AG
SnaBI 112 TACGTA TAC^GTA
XcmI 242 CCATGAACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
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AfaI 115 GTAC GT^AC
591 GTAC
AlwI 255 GGATC GGATC(4/5)
692 GGATC
AlwNI 85 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
298 CAGCTCCTG
Aor51HI 107 AGCGCT AGC^GCT
212 AGCGCT
AvaI 38 CCCGGG C^[CT]CG[GA]G
809 CCCGGG
BanI 22 GGCACC G^G[CT][GA]CC
646 GGTGCC
BsmAI 343 GAGAC GTCTC(1/5)
394 GTCTC
BsoBI 38 CCCGGG C^[CT]CG[GA]G
809 CCCGGG
DraII 449 GGGACCT [GA]G^G[ACGT]CC[CT]
828 AGGGCCC
DrdI 70 GACCAGCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
385 GACCACGCTGTC
DsaI 55 CCGCGG C^C[GA][CT]GG
611 CCACGG
Eco47III 107 AGCGCT AGC^GCT
212 AGCGCT
EcoO109I 449 GGGACCT [GA]G^G[ACGT]CC[CT]
828 AGGGCCC
MaeIII 515 GTGAC ^GT[ACGT]AC
859 GTCAC
MboII 171 GAAGA GAAGA(8/7)
230 TCTTC
MspA1I 55 CCGCGG C[AC]G^C[GT]G
145 CAGCGG
PstI 82 CTGCAG CTGCA^G
629 CTGCAG
RsaI 115 GTAC GT^AC
591 GTAC
SmaI 38 CCCGGG CCC^GGG
809 CCCGGG
SphI 317 GCATGC GCATG^C
540 GCATGC
StuI 524 AGGCCT AGG^CCT
639 AGGCCT
TaqI 362 TCGA T^CGA
740 TCGA
Tsp45I 515 GTGAC ^GT[GC]AC
859 GTCAC
TthHB8I 362 TCGA T^CGA
740 TCGA
XmaCI 38 CCCGGG C^CCGGG
809 CCCGGG
XmaI 38 CCCGGG C^CCGGG
809 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AccII AciI AluI AvaII BanII
BbvI BcnI BmyI BpmI BsaJI
BseRI BsgI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HpaII ItaI MboI MnlI
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TseI TspRI