Restriction map for
pFN21AB9359
(Linear) (Six-base) MAPSORT of: KIBB9359.seq Check: 4147 from: 1 to: 876
KIBB9359.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 725 876
Size: 725 151
AfeI AGC'GCT
Cuts at: 0 109 214 876
Size: 109 105 662
Fragments arranged by size:
662 109 105
AleI CACnn'nnGTG
Cuts at: 0 706 876
Size: 706 170
AlwNI CAG_nnn'CTG
Cuts at: 0 90 303 876
Size: 90 213 573
Fragments arranged by size:
573 213 90
ApaI G_GGCC'C
Cuts at: 0 833 876
Size: 833 43
AvaI C'yCGr_G
Cuts at: 0 38 809 876
Size: 38 771 67
Fragments arranged by size:
771 67 38
AvrII C'CTAG_G
Cuts at: 0 432 876
Size: 432 444
BanI G'GyrC_C
Cuts at: 0 22 646 876
Size: 22 624 230
Fragments arranged by size:
624 230 22
BanII G_rGCy'C
Cuts at: 0 674 817 833 876
Size: 674 143 16 43
Fragments arranged by size:
674 143 43 16
BbsI GAAGACnn'nnnn_
Cuts at: 0 222 876
Size: 222 654
Bme1580I G_kGCm'C
Cuts at: 0 833 876
Size: 833 43
BmrI ACTGGGnnnn_n'
Cuts at: 0 426 876
Size: 426 450
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 214 771 801 876
Size: 121 93 557 30 75
Fragments arranged by size:
557 121 93 75 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 876
Size: 427 449
BsaI GGTCTCn'nnnn_
Cuts at: 0 337 876
Size: 337 539
BsaAI yAC'GTr
Cuts at: 0 114 876
Size: 114 762
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 25 831 861 876
Size: 25 806 30 15
Fragments arranged by size:
806 30 25 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 118 220 235 876
Size: 118 102 15 641
Fragments arranged by size:
641 118 102 15
BseYI C'CCAG_C
Cuts at: 0 63 792 832 876
Size: 63 729 40 44
Fragments arranged by size:
729 63 44 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 478 731 876
Size: 163 315 253 145
Fragments arranged by size:
315 253 163 145
BsiHKAI G_wGCw'C
Cuts at: 0 674 876
Size: 674 202
Bsp1286I G_dGCh'C
Cuts at: 0 674 817 833 876
Size: 674 143 16 43
Fragments arranged by size:
674 143 43 16
BsrBI CCG'CTC
Cuts at: 0 775 876
Size: 775 101
BsrFI r'CCGG_y
Cuts at: 0 497 876
Size: 497 379
BstAPI GCAn_nnn'nTGC
Cuts at: 0 90 876
Size: 90 786
Bsu36I CC'TnA_GG
Cuts at: 0 763 876
Size: 763 113
BtgI C'CryG_G
Cuts at: 0 55 611 876
Size: 55 556 265
Fragments arranged by size:
556 265 55
BtsI GCAGTG_nn'
Cuts at: 0 638 876
Size: 638 238
DrdI GACnn_nn'nnGTC
Cuts at: 0 76 391 876
Size: 76 315 485
Fragments arranged by size:
485 315 76
EaeI y'GGCC_r
Cuts at: 0 239 876
Size: 239 637
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 85 876
Size: 85 791
EcoICRI GAG'CTC
Cuts at: 0 672 876
Size: 672 204
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 214 725 771 801 876
Size: 121 93 511 46 30 75
Fragments arranged by size:
511 121 93 75 46 30
EcoO109I rG'GnC_Cy
Cuts at: 0 450 829 876
Size: 450 379 47
Fragments arranged by size:
450 379 47
HaeII r_GCGC'y
Cuts at: 0 111 216 799 876
Size: 111 105 583 77
Fragments arranged by size:
583 111 105 77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 831 863 876
Size: 831 32 13
Fragments arranged by size:
831 32 13
MscI TGG'CCA
Cuts at: 0 241 876
Size: 241 635
MslI CAynn'nnrTG
Cuts at: 0 706 876
Size: 706 170
MspA1I CmG'CkG
Cuts at: 0 57 147 876
Size: 57 90 729
Fragments arranged by size:
729 90 57
NspI r_CATG'y
Cuts at: 0 168 321 544 876
Size: 168 153 223 332
Fragments arranged by size:
332 223 168 153
PpuMI rG'GwC_Cy
Cuts at: 0 450 876
Size: 450 426
PspOMI G'GGCC_C
Cuts at: 0 829 876
Size: 829 47
PstI C_TGCA'G
Cuts at: 0 86 633 876
Size: 86 547 243
Fragments arranged by size:
547 243 86
SacI G_AGCT'C
Cuts at: 0 674 876
Size: 674 202
SacII CC_GC'GG
Cuts at: 0 58 876
Size: 58 818
SfcI C'TryA_G
Cuts at: 0 82 569 629 851 876
Size: 82 487 60 222 25
Fragments arranged by size:
487 222 82 60 25
SmaI CCC'GGG
Cuts at: 0 40 811 876
Size: 40 771 65
Fragments arranged by size:
771 65 40
SmlI C'TyrA_G
Cuts at: 0 442 876
Size: 442 434
SnaBI TAC'GTA
Cuts at: 0 114 876
Size: 114 762
SphI G_CATG'C
Cuts at: 0 321 544 876
Size: 321 223 332
Fragments arranged by size:
332 321 223
StuI AGG'CCT
Cuts at: 0 526 641 876
Size: 526 115 235
Fragments arranged by size:
526 235 115
StyI C'CwwG_G
Cuts at: 0 432 527 825 876
Size: 432 95 298 51
Fragments arranged by size:
432 298 95 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 295 533 634 876
Size: 295 238 101 242
Fragments arranged by size:
295 242 238 101
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 27 461 493 876
Size: 27 434 32 383
Fragments arranged by size:
434 383 32 27
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 249 876
Size: 249 627
XmaI C'CCGG_G
Cuts at: 0 38 809 876
Size: 38 771 67
Fragments arranged by size:
771 67 38
Enzymes that do cut:
AcuI AfeI AleI AlwNI ApaI AvaI AvrII BanI
BanII BbsI Bme1580I BmrI BpmI BpuEI BsaI BsaAI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BsrBI BsrFI
BstAPI Bsu36I BtgI BtsI DrdI EaeI EciI EcoICRI
Eco57MI EcoO109I HaeII Hin4I MscI MslI MspA1I NspI
PpuMI PspOMI PstI SacI SacII SfcI SmaI SmlI
SnaBI SphI StuI StyI TaqII TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AlfI AloI ApaLI ApoI AscI AseI AsiSI
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmgBI BmtI BplI Bpu10I BsaBI
BsaHI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrDI BsrGI BssHII BssSI BstBI BstEII BstXI
BstYI BstZ17I BtgZI ClaI CspCI DraI DraIII EagI
EarI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI PvuII RsrII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SpeI SrfI SspI SwaI TatI Tth111I
XbaI XhoI XmnI ZraI