################################## # NO CUT ENZYMES ( 66 enzymes) # ################################## AccI AclI AflII ApaI AscI AseI AsnI AspI BamHI BciVI BfrI BlpI Bpu1102I BsaBI BsiWI BsmBI BsmI BspHI BspLU11I Bst1107I BstBI BstEII BstPI BstZ17I CelII CpoI DrdI EcoO65I EcoRI EcoT22I FseI HincII HindII HpaI MamI MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PmeI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 65 enzymes) # ################################### Acc65I 1532 GGTACC G^GTACC AccIII 13 TCCGGA T^CCGGA AflIII 652 ACGTGT A^C[GA][CT]GT AgeI 1529 ACCGGT A^CCGGT AhdI 3577 GACGTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 669 GTGCAC G^TGCAC Aor51HI 2941 AGCGCT AGC^GCT ApaLI 669 GTGCAC G^TGCAC Asp718I 1532 GGTACC G^GTACC AspEI 3577 GACGTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 669 GTGCAC G[AT]GC[AT]^C AviII 3731 TGCGCA TGC^GCA AvrII 1224 CCTAGG C^CTAGG BbeI 1409 GGCGCC GGCGC^C BbrPI 651 CACGTG CAC^GTG BbvCI 855 CCTCAGC CCTCAGC(-5/-2) BclI 2804 TGATCA T^GATCA BglII 1799 AGATCT A^GATCT BlnI 1224 CCTAGG C^CTAGG BseAI 13 TCCGGA T^CCGGA BsiEI 2273 CGGCCG CG[GA][CT]^CG BsiHKAI 669 GTGCAC G[AT]GC[AT]^C Bsp1407I 1805 TGTACA T^GTACA BspDI 2401 ATCGAT AT^CGAT BspEI 13 TCCGGA T^CCGGA BspMI 4093 ACCTGC ACCTGC(4/8) BsrBI 4063 GAGCGG CCGCTC(-3/-3) BsrDI 1107 CATTGC GCAATG(2/0) BsrGI 1805 TGTACA T^GTACA BssHII 719 GCGCGC G^CGCGC BssSI 2422 CACGAG CACGAG(-5/-1) BstAPI 953 GCATGTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC ClaI 2401 ATCGAT AT^CGAT DraI 2547 TTTAAA TTT^AAA DraIII 3175 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 2273 CGGCCG C^GGCCG Eam1105I 3577 GACGTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 2273 CGGCCG C^GGCCG Eco47III 2941 AGCGCT AGC^GCT Eco52I 2273 CGGCCG C^GGCCG FbaI 2804 TGATCA T^GATCA FspI 3731 TGCGCA TGC^GCA HindIII 3146 AAGCTT A^AGCTT KasI 1409 GGCGCC G^GCGCC KpnI 1532 GGTACC GGTAC^C KspI 318 CCGCGG CCGC^GG MfeI 3168 CAATTG C^AATTG MroI 13 TCCGGA T^CCGGA MunI 3168 CAATTG C^AATTG NarI 1409 GGCGCC GG^CGCC PaeR7I 277 CTCGAG C^TCGAG PflMI 1637 CCAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 1529 ACCGGT A^CCGGT PmaCI 651 CACGTG CAC^GTG PmlI 651 CACGTG CAC^GTG SacII 318 CCGCGG CCGC^GG SapI 2305 GAAGAGC GCTCTTC(1/4) SfoI 1409 GGCGCC GGC^GCC SmaI 2691 CCCGGG CCC^GGG SspBI 1805 TGTACA T^GTACA Van91I 1637 CCAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1673 CCAACAAGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 277 CTCGAG C^TCGAG XmaCI 2691 CCCGGG C^CCGGG XmaI 2691 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AatII 1471 GACGTC GACGT^C 3577 GACGTC AcsI 1373 AAATTT [GA]^AATT[CT] 2169 GAATTT AocI 396 CCTGAGG CC^T[ACGT]AGG 1954 CCTCAGG ApoI 1373 AAATTT [GA]^AATT[CT] 2169 GAATTT Asp700I 327 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3159 GAAGGAGTTC BalI 2234 TGGCCA TGG^CCA 3086 TGGCCA BcgI 155 GCACGGTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3654 GCAAGTCCTTCG BglI 920 GCCCCTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3613 GCCACACTGGC BsaAI 651 CACGTG [CT]AC^GT[GA] 1159 TACGTG BsgI 153 CTGCAC GTGCAG(16/14) 2176 CTGCAC Bsu36I 396 CCTGAGG CC^T[ACGT]AGG 1954 CCTCAGG Eco81I 396 CCTGAGG CC^T[ACGT]AGG 1954 CCTCAGG EcoNI 967 CCTTGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1224 CCTAGGAGAGG EcoRV 2404 GATATC GAT^ATC 3472 GATATC HgaI 3465 GACGC GACGC(5/10) 3902 GCGTC MluNI 2234 TGGCCA TGG^CCA 3086 TGGCCA MscI 2234 TGGCCA TGG^CCA 3086 TGGCCA NcoI 554 CCATGG C^CATGG 1760 CCATGG PshAI 538 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3700 GACAACTGTC PstI 714 CTGCAG CTGCA^G 2442 CTGCAG ScaI 248 AGTACT AGT^ACT 2933 AGTACT SphI 908 GCATGC GCATG^C 3116 GCATGC StuI 2527 AGGCCT AGG^CCT 3763 AGGCCT XmnI 327 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3159 GAAGGAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 103 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaI BsaJI BsaWI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII