Restriction map for
pFN21AB5702
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflII
AgeI AhdI Alw44I ApaLI AscI
Asp718I AspEI AspHI AspI AviII
AvrII BbeI BcgI BciVI BfrI
BglII BlnI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI Bsp1407I BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstEII BstPI BstXI BstZ17I
Cfr10I CpoI DraIII DrdI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI Hin1I
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MroI MunI
NaeI NarI NgoMIV NheI NotI
NruI NsiI PacI PflFI PinAI
PmeI PvuI RcaI RsrII SacI
SacII SalI ScaI SexAI SfiI
SfoI SgrAI SnaBI SpeI SspBI
SspI SwaI Tth111I XcmI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 974 CGCG CG^CG
AclI 649 AACGTT AA^CGTT
AcsI 210 GAATTT [GA]^AATT[CT]
AfaI 1050 GTAC GT^AC
AflIII 973 ACGCGT A^C[GA][CT]GT
Aor51HI 896 AGCGCT AGC^GCT
ApaI 136 GGGCCC GGGCC^C
ApoI 210 GAATTT [GA]^AATT[CT]
AseI 1393 ATTAAT AT^TAAT
AsnI 1393 ATTAAT AT^TAAT
Asp700I 283 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 2586 GGCACC G^G[CT][GA]CC
BanII 136 GGGCCC G[GA]GC[CT]^C
BbrPI 2668 CACGTG CAC^GTG
BclI 612 TGATCA T^GATCA
BlpI 2020 GCTCAGC GC^T[ACGT]AGC
BpmI 913 CTGGAG CTGGAG(16/14)
Bpu1102I 2020 GCTCAGC GC^T[ACGT]AGC
BspDI 1834 ATCGAT AT^CGAT
BstUI 974 CGCG CG^CG
CelII 2020 GCTCAGC GC^T[ACGT]AGC
ClaI 1834 ATCGAT AT^CGAT
DraI 1459 TTTAAA TTT^AAA
Eco47III 896 AGCGCT AGC^GCT
EcoNI 2451 CCTGATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1814 CCATGG C^C[AT][AT]GG
FbaI 612 TGATCA T^GATCA
MluI 973 ACGCGT A^CGCGT
MvnI 974 CGCG CG^CG
NcoI 1814 CCATGG C^CATGG
NdeI 836 CATATG CA^TATG
PaeR7I 383 CTCGAG C^TCGAG
PmaCI 2668 CACGTG CAC^GTG
PmlI 2668 CACGTG CAC^GTG
PshBI 1393 ATTAAT AT^TAAT
Psp1406I 649 AACGTT AA^CGTT
RsaI 1050 GTAC GT^AC
SapI 347 GCTCTTC GCTCTTC(1/4)
SbfI 1029 CCTGCAGG CCTGCA^GG
SphI 679 GCATGC GCATG^C
Sse8387I 1029 CCTGCAGG CCTGCA^GG
StuI 904 AGGCCT AGG^CCT
StyI 1814 CCATGG C^C[AT][AT]GG
XbaI 51 TCTAGA T^CTAGA
XhoI 383 CTCGAG C^TCGAG
XmnI 283 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AocI 1384 CCTCAGG CC^T[ACGT]AGG
1903 CCTGAGG
BamHI 39 GGATCC G^GATCC
2257 GGATCC
BbsI 1119 GAAGAC GAAGAC(2/6)
1975 GAAGAC
BfaI 52 CTAG C^TAG
629 CTAG
BmyI 136 GGGCCC G[AGT]GC[ACT]^C
2003 GTGCCC
BpuAI 1119 GAAGAC GAAGAC(2/6)
1975 GAAGAC
BsaAI 1660 CACGTA [CT]AC^GT[GA]
2668 CACGTG
BsmI 495 GAATGC GAATGC(1/-1)
2478 GAATGC
Bsp1286I 136 GGGCCC G[AGT]GC[ACT]^C
2003 GTGCCC
BstAPI 1032 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2319 GCAGGACCTGC
BstBI 290 TTCGAA TT^CGAA
1349 TTCGAA
Bsu36I 1384 CCTCAGG CC^T[ACGT]AGG
1903 CCTGAGG
CfoI 897 GCGC GCG^C
2441 GCGC
DsaI 1781 CCGTGG C^C[GA][CT]GG
1814 CCATGG
Eco81I 1384 CCTCAGG CC^T[ACGT]AGG
1903 CCTGAGG
FauI 1844 CCCGC CCCGC(4/6)
2411 GCGGG
HaeII 896 AGCGCT [GA]GCGC^[CT]
2440 AGCGCC
HgaI 921 GCGTC GACGC(5/10)
1964 GCGTC
HhaI 897 GCGC GCG^C
2441 GCGC
HinP1I 897 GCGC G^CGC
2441 GCGC
HindIII 1342 AAGCTT A^AGCTT
2272 AAGCTT
MaeI 52 CTAG C^TAG
629 CTAG
NspI 679 GCATGC [GA]CATG^[CT]
1275 ACATGC
NspV 290 TTCGAA TT^CGAA
1349 TTCGAA
PflMI 174 CCATGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1809 CCAGCCCATGG
PshAI 3 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1959 GACGAGCGTC
SfuI 290 TTCGAA TT^CGAA
1349 TTCGAA
SmaI 1649 CCCGGG CCC^GGG
2006 CCCGGG
Van91I 174 CCATGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1809 CCAGCCCATGG
XmaCI 1649 CCCGGG C^CCGGG
2006 CCCGGG
XmaI 1649 CCCGGG C^CCGGG
2006 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccI AciI AluI AlwI AlwNI
AvaI AvaII BalI BbvCI BbvI
BcnI BglI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsoBI
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII