Restriction map for pFN21AB5702
 (Linear) (Six-base) MAPSORT of: KIBB5702.seq  Check: 5833  from: 1  to: 2718



KIBB5702.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:28  ..

AccI GT'mk_AC

Cuts at:      0     440    1137    1700    2718 
   Size:        440     697     563    1018

  Fragments arranged by size: 

                1018     697     563     440
 
AclI AA'CG_TT

Cuts at:      0     650    2718 
   Size:        650    2068
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     566     870    2120    2718 
   Size:        566     304    1250     598

  Fragments arranged by size: 

                1250     598     566     304
 
AfeI AGC'GCT

Cuts at:      0     898    2718 
   Size:        898    1820
 
AflIII A'CryG_T

Cuts at:      0     973    2718 
   Size:        973    1745
 
AleI CACnn'nnGTG

Cuts at:      0     812    2718 
   Size:        812    1906
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2313    2718 
   Size:       2313     405
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1029    1038    1501    2325    2718 
   Size:       1029       9     463     824     393

  Fragments arranged by size: 

                1029     824     463     393       9
 
ApaI G_GGCC'C

Cuts at:      0     140    2718 
   Size:        140    2578
 
ApoI r'AATT_y

Cuts at:      0     210    2718 
   Size:        210    2508
 
AseI AT'TA_AT

Cuts at:      0    1394    2718 
   Size:       1394    1324
 
AvaI C'yCGr_G

Cuts at:      0     383    1649    2006    2189    2718 
   Size:        383    1266     357     183     529

  Fragments arranged by size: 

                1266     529     383     357     183
 
BamHI G'GATC_C

Cuts at:      0      39    2257    2718 
   Size:         39    2218     461

  Fragments arranged by size: 

                2218     461      39
 
BanI G'GyrC_C

Cuts at:      0    2586    2718 
   Size:       2586     132
 
BanII G_rGCy'C

Cuts at:      0     140    2718 
   Size:        140    2578
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1126    1982    2718 
   Size:       1126     856     736

  Fragments arranged by size: 

                1126     856     736
 
BbvCI CC'TCA_GC

Cuts at:      0    1792    2249    2689    2718 
   Size:       1792     457     440      29

  Fragments arranged by size: 

                1792     457     440      29
 
BclI T'GATC_A

Cuts at:      0     612    2718 
   Size:        612    2106
 
BglI GCCn_nnn'nGGC

Cuts at:      0     712     955    1306    2718 
   Size:        712     243     351    1412

  Fragments arranged by size: 

                1412     712     351     243
 
BlpI GC'TnA_GC

Cuts at:      0    2021    2718 
   Size:       2021     697
 
Bme1580I G_kGCm'C

Cuts at:      0     140    2007    2718 
   Size:        140    1867     711

  Fragments arranged by size: 

                1867     711     140
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     409    2718 
   Size:        409    2309
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     934    2718 
   Size:        934    1784
 
Bpu10I CC'TnA_GC

Cuts at:      0    1792    2249    2689    2718 
   Size:       1792     457     440      29

  Fragments arranged by size: 

                1792     457     440      29
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     241     900    2718 
   Size:        241     659    1818

  Fragments arranged by size: 

                1818     659     241
 
BsaAI yAC'GTr

Cuts at:      0    1662    2670    2718 
   Size:       1662    1008      48

  Fragments arranged by size: 

                1662    1008      48
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     235     265    2718 
   Size:        235      30    2453

  Fragments arranged by size: 

                2453     235      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     277     833     836     839     907     931    1921    2718 
   Size:        277     556       3       3      68      24     990     797

  Fragments arranged by size: 

                 990     797     556     277      68      24       3       3
            
 
BseYI C'CCAG_C

Cuts at:      0    1808    2718 
   Size:       1808     910
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     954    1385    2419    2509    2701    2718 
   Size:        954     431    1034      90     192      17

  Fragments arranged by size: 

                1034     954     431     192      90      17
 
BsmI GAATG_Cn'

Cuts at:      0     501    2484    2718 
   Size:        501    1983     234

  Fragments arranged by size: 

                1983     501     234
 
Bsp1286I G_dGCh'C

Cuts at:      0     140    2007    2718 
   Size:        140    1867     711

  Fragments arranged by size: 

                1867     711     140
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1046    2333    2345    2718 
   Size:       1046    1287      12     373

  Fragments arranged by size: 

                1287    1046     373      12
 
BsrDI GCAATG_nn'

Cuts at:      0     510    1065    1580    2133    2285    2295    2718 
   Size:        510     555     515     553     152      10     423

  Fragments arranged by size: 

                 555     553     515     510     423     152      10
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1038    2325    2718 
   Size:       1038    1287     393

  Fragments arranged by size: 

                1287    1038     393
 
BstBI TT'CG_AA

Cuts at:      0     291    1350    2718 
   Size:        291    1059    1368

  Fragments arranged by size: 

                1368    1059     291
 
BstYI r'GATC_y

Cuts at:      0      39    1452    2257    2718 
   Size:         39    1413     805     461

  Fragments arranged by size: 

                1413     805     461      39
 
Bsu36I CC'TnA_GG

Cuts at:      0    1385    1904    2718 
   Size:       1385     519     814

  Fragments arranged by size: 

                1385     814     519
 
BtgI C'CryG_G

Cuts at:      0    1781    1814    2718 
   Size:       1781      33     904

  Fragments arranged by size: 

                1781     904      33
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2331    2718 
   Size:       2331     387
 
BtsI GCAGTG_nn'

Cuts at:      0     782    1889    2718 
   Size:        782    1107     829

  Fragments arranged by size: 

                1107     829     782
 
ClaI AT'CG_AT

Cuts at:      0    1835    2718 
   Size:       1835     883
 
DraI TTT'AAA

Cuts at:      0    1461    2718 
   Size:       1461    1257
 
EaeI y'GGCC_r

Cuts at:      0     603     956    1995    2063    2144    2277    2718 
   Size:        603     353    1039      68      81     133     441

  Fragments arranged by size: 

                1039     603     441     353     133      81      68
 
EarI CTCTTCn'nnn_

Cuts at:      0     145     354    2605    2718 
   Size:        145     209    2251     113

  Fragments arranged by size: 

                2251     209     145     113
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1009    2718 
   Size:       1009    1709
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     566     870     934    2120    2718 
   Size:        566     304      64    1186     598

  Fragments arranged by size: 

                1186     598     566     304      64
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2455    2718 
   Size:       2455     263
 
EcoO109I rG'GnC_Cy

Cuts at:      0     136     137     723    1026    1035    1898    2322    2685 
   Size:        136       1     586     303       9     863     424     363

Cuts at:   2685    2718 
   Size:         33

  Fragments arranged by size: 

                 863     586     424     363     303     136      33       9
                   1
 
HaeII r_GCGC'y

Cuts at:      0     900    2444    2718 
   Size:        900    1544     274

  Fragments arranged by size: 

                1544     900     274
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      48      80     331     363     784     816    2004    2036 
   Size:         48      32     251      32     421      32    1188      32

Cuts at:   2036    2242    2274    2718 
   Size:        206      32     444

  Fragments arranged by size: 

                1188     444     421     251     206      48      32      32
                  32      32      32
 
HindIII A'AGCT_T

Cuts at:      0    1342    2272    2718 
   Size:       1342     930     446

  Fragments arranged by size: 

                1342     930     446
 
MluI A'CGCG_T

Cuts at:      0     973    2718 
   Size:        973    1745
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      65    1763    1892    2718 
   Size:         65    1698     129     826

  Fragments arranged by size: 

                1698     826     129      65
 
MscI TGG'CCA

Cuts at:      0     605    1997    2065    2146    2718 
   Size:        605    1392      68      81     572

  Fragments arranged by size: 

                1392     605     572      81      68
 
MslI CAynn'nnrTG

Cuts at:      0     695     812    1747    2137    2164    2718 
   Size:        695     117     935     390      27     554

  Fragments arranged by size: 

                 935     695     554     390     117      27
 
MspA1I CmG'CkG

Cuts at:      0     459     741    1624    1986    2367    2718 
   Size:        459     282     883     362     381     351

  Fragments arranged by size: 

                 883     459     381     362     351     282
 
NcoI C'CATG_G

Cuts at:      0    1814    2718 
   Size:       1814     904
 
NdeI CA'TA_TG

Cuts at:      0     837    2718 
   Size:        837    1881
 
NspI r_CATG'y

Cuts at:      0     683    1279    2718 
   Size:        683     596    1439

  Fragments arranged by size: 

                1439     683     596
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     180    1815    2718 
   Size:        180    1635     903

  Fragments arranged by size: 

                1635     903     180
 
PfoI T'CCnGG_A

Cuts at:      0    1181    1865    2072    2260    2718 
   Size:       1181     684     207     188     458

  Fragments arranged by size: 

                1181     684     458     207     188
 
PmlI CAC'GTG

Cuts at:      0    2670    2718 
   Size:       2670      48
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     144     176    2718 
   Size:        144      32    2542

  Fragments arranged by size: 

                2542     144      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     723    1035    1898    2322    2718 
   Size:        723     312     863     424     396

  Fragments arranged by size: 

                 863     723     424     396     312
 
PshAI GACnn'nnGTC

Cuts at:      0       7    1963    2718 
   Size:          7    1956     755

  Fragments arranged by size: 

                1956     755       7
 
PspOMI G'GGCC_C

Cuts at:      0     136    2718 
   Size:        136    2582
 
PspXI vC'TCGA_Gb

Cuts at:      0     383    2718 
   Size:        383    2335
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     272     304    1331    1363    2718 
   Size:        272      32    1027      32    1355

  Fragments arranged by size: 

                1355    1027     272      32      32
 
PstI C_TGCA'G

Cuts at:      0     324     627     644     746    1034    2380    2718 
   Size:        324     303      17     102     288    1346     338

  Fragments arranged by size: 

                1346     338     324     303     288     102      17
 
PvuII CAG'CTG

Cuts at:      0     459     741    1624    1986    2367    2718 
   Size:        459     282     883     362     381     351

  Fragments arranged by size: 

                 883     459     381     362     351     282
 
SapI GCTCTTCn'nnn_

Cuts at:      0     354    2718 
   Size:        354    2364
 
SbfI CC_TGCA'GG

Cuts at:      0    1034    2718 
   Size:       1034    1684
 
SfcI C'TryA_G

Cuts at:      0     278     320     623     640     685     742    1030    2376 
   Size:        278      42     303      17      45      57     288    1346

Cuts at:   2376    2718 
   Size:        342

  Fragments arranged by size: 

                1346     342     303     288     278      57      45      42
                  17
 
SmaI CCC'GGG

Cuts at:      0    1651    2008    2718 
   Size:       1651     357     710

  Fragments arranged by size: 

                1651     710     357
 
SmlI C'TyrA_G

Cuts at:      0     256     383     879    2718 
   Size:        256     127     496    1839

  Fragments arranged by size: 

                1839     496     256     127
 
SphI G_CATG'C

Cuts at:      0     683    2718 
   Size:        683    2035
 
SrfI GCCC'GGGC

Cuts at:      0    2008    2718 
   Size:       2008     710
 
StuI AGG'CCT

Cuts at:      0     906    2718 
   Size:        906    1812
 
StyI C'CwwG_G

Cuts at:      0    1814    2718 
   Size:       1814     904
 
TatI w'GTAC_w

Cuts at:      0    1049    2718 
   Size:       1049    1669
 
XbaI T'CTAG_A

Cuts at:      0      51    2718 
   Size:         51    2667
 
XhoI C'TCGA_G

Cuts at:      0     383    2718 
   Size:        383    2335
 
XmaI C'CCGG_G

Cuts at:      0    1649    2006    2718 
   Size:       1649     357     712

  Fragments arranged by size: 

                1649     712     357
 
XmnI GAAnn'nnTTC

Cuts at:      0     287    2718 
   Size:        287    2431
 

 Enzymes that do cut:

     AccI     AclI     AcuI     AfeI   AflIII     AleI     AlfI    AlwNI
     ApaI     ApoI     AseI     AvaI    BamHI     BanI    BanII     BbsI
    BbvCI     BclI     BglI     BlpI Bme1580I     BplI     BpmI   Bpu10I
    BpuEI    BsaAI    BsaXI    BseRI    BseYI     BsgI     BsmI Bsp1286I
    BspMI    BsrDI   BstAPI    BstBI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     ClaI     DraI     EaeI     EarI     EciI  Eco57MI    EcoNI
 EcoO109I    HaeII    Hin4I  HindIII     MluI     MmeI     MscI     MslI
   MspA1I     NcoI     NdeI     NspI    PflMI     PfoI     PmlI     PpiI
    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI    PvuII     SapI
     SbfI     SfcI     SmaI     SmlI     SphI     SrfI     StuI     StyI
     TatI     XbaI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I    AflII     AgeI     AhdI     AloI    ApaLI
     AscI    AsiSI    AvrII     BaeI     BbeI     BcgI    BciVI    BfrBI
    BglII    BmgBI     BmrI     BmtI     BsaI    BsaBI    BsaHI    BsaWI
    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstEII    BstXI  BstZ17I    CspCI   DraIII
     DrdI     EagI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI   HincII     HpaI     KasI     KpnI     MfeI     NaeI     NarI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
     PmeI     PsiI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI
     SspI     SwaI    TaqII     TstI  Tth111I     XcmI     ZraI
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