Restriction map for
pFN21AB5676
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII Acc65I AclI AcyI AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI BamHI BanII BbeI
BbrPI BbvCI BcgI BcnI BglI
BglII BlpI Bpu1102I BsaAI BsaBI
BsaHI BsgI BsiEI BsiWI Bsp1407I
BspDI BsrGI BstAPI BstBI Bsu36I
CelII ClaI CpoI DraI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRV EcoT22I FseI Hin1I HindIII
HpaI KasI KpnI KspI MamI
MluI MspA1I NaeI NarI NciI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Van91I XbaI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 2228 CGCG CG^CG
AccIII 437 TCCGGA T^CCGGA
AflII 403 CTTAAG C^TTAAG
AflIII 1017 ACATGT A^C[GA][CT]GT
AspI 1792 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 764 CTCGAG C^[CT]CG[GA]G
AviII 1982 TGCGCA TGC^GCA
AvrII 285 CCTAGG C^CTAGG
BanI 1410 GGTGCC G^G[CT][GA]CC
BfrI 403 CTTAAG C^TTAAG
BlnI 285 CCTAGG C^CTAGG
BsaI 1303 GAGACC GGTCTC(1/5)
BseAI 437 TCCGGA T^CCGGA
BseRI 871 GAGGAG GAGGAG(10/8)
BsmBI 189 GAGACG CGTCTC(1/5)
BsoBI 764 CTCGAG C^[CT]CG[GA]G
BspEI 437 TCCGGA T^CCGGA
BspLU11I 1017 ACATGT A^CATGT
BspMI 1979 ACCTGC ACCTGC(4/8)
BsrBI 961 GAGCGG CCGCTC(-3/-3)
BsrDI 1590 CATTGC GCAATG(2/0)
BsrFI 1871 ACCGGC [GA]^CCGG[CT]
BssHII 2227 GCGCGC G^CGCGC
BssSI 1754 CTCGTG CACGAG(-5/-1)
BstEII 1419 GGTAACC G^GT[ACGT]ACC
BstPI 1419 GGTAACC G^GT[ACGT]ACC
BstUI 2228 CGCG CG^CG
BstXI 2000 CCAAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1871 ACCGGC [GA]^CCGG[CT]
DraIII 1644 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1904 GACCTGGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1419 GGTAACC G^GT[ACGT]ACC
EcoRI 1109 GAATTC G^AATTC
FspI 1982 TGCGCA TGC^GCA
HaeII 1861 GGCGCT [GA]GCGC^[CT]
HgaI 1127 GACGC GACGC(5/10)
HincII 984 GTTGAC GT[CT]^[GA]AC
HindII 984 GTTGAC GT[CT]^[GA]AC
MfeI 695 CAATTG C^AATTG
MroI 437 TCCGGA T^CCGGA
MunI 695 CAATTG C^AATTG
MvnI 2228 CGCG CG^CG
NcoI 2156 CCATGG C^CATGG
NheI 636 GCTAGC G^CTAGC
PaeR7I 764 CTCGAG C^TCGAG
PflFI 1792 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1629 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 624 CTGCAG CTGCA^G
Tth111I 1792 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 764 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 397 GTATAC GT^[AC][GT]AC
2383 GTATAC
AcsI 1109 GAATTC [GA]^AATT[CT]
1504 AAATTC
ApoI 1109 GAATTC [GA]^AATT[CT]
1504 AAATTC
AspHI 1242 GTGCTC G[AT]GC[AT]^C
1492 GAGCAC
BalI 1580 TGGCCA TGG^CCA
2090 TGGCCA
BbsI 1429 GAAGAC GAAGAC(2/6)
2392 GAAGAC
BciVI 338 GTATCC GTATCC(6/5)
1482 GTATCC
BclI 803 TGATCA T^GATCA
1917 TGATCA
BpuAI 1429 GAAGAC GAAGAC(2/6)
2392 GAAGAC
BsaWI 437 TCCGGA [AT]^CCGG[AT]
1355 TCCGGT
BsiHKAI 1242 GTGCTC G[AT]GC[AT]^C
1492 GAGCAC
BsmI 885 GAATGC GAATGC(1/-1)
1600 GAATGC
BspHI 821 TCATGA T^CATGA
1322 TCATGA
Bst1107I 397 GTATAC GTA^TAC
2383 GTATAC
BstZ17I 397 GTATAC GTA^TAC
2383 GTATAC
DraII 1439 GGGGCCT [GA]G^G[ACGT]CC[CT]
1629 GGGTCCT
EcoO109I 1439 GGGGCCT [GA]G^G[ACGT]CC[CT]
1629 GGGTCCT
FauI 1697 CCCGC CCCGC(4/6)
1392 GCGGG
FbaI 803 TGATCA T^GATCA
1917 TGATCA
MaeII 336 ACGT A^CGT
1458 ACGT
MluNI 1580 TGGCCA TGG^CCA
2090 TGGCCA
MscI 1580 TGGCCA TGG^CCA
2090 TGGCCA
MslI 1210 CACGGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1216 CATGTGGGTG
RcaI 821 TCATGA T^CATGA
1322 TCATGA
Tsp45I 161 GTGAC ^GT[GC]AC
2243 GTGAC
XcmI 1414 CCACAGGTAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2331 CCAGGGTGGAGCTGG
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# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BbvI BfaI BmyI BpmI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EaeI EarI EcoNI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII